利用牙鲆鳃细胞系分离和培养淋巴囊肿病毒

本文利用牙鲆鳃细胞系进行了养殖牙鲆淋巴囊肿病毒的分离及培养 ,并通过电镜对培养细胞中淋巴囊肿病毒的形态及感染循环进行了初步研究。将病鱼的淋巴囊肿组织无菌滤液接种牙鲆细胞系 ,细胞出现了明显的细胞病变 ( Cytopathic effect,CPE)。电镜观察在培养细胞的胞质中有病毒的包涵体 ,胞质中散在 6角形、5角形或圆形的病毒粒子 ,大小为 10 0～ 140 nm之间。在感染细胞的线粒体中也存在大量的病毒颗粒。     

Diagnosis of iridovirus in large yellow croaker by PCR

A rapid and sensitive PCR-based method for the detection of the large yellow croaker iridovirus(LYCIV) is described,which involves the amplification of a 295 bp fragment of the LYCIV ATPase gene from DNA isolated from naturally infected fish spleen.Sequencing of LYCIV ATPase gene fragment showed it shared 100% nucleotide sequence homology with the corresponding region of the ATPase gene of red sea bream iridovirus(RSIV) and sea bass iridovirus(SBIV),suggesting that LYCIV was homologous with RSIV and SBIV at least in part of the gemone.The specificity and sensitivity of the PCR procedure were tested on the iridovirus-infected fishes,the expected fragment was detected from spleen DNA samples of infected fishes,whereas no fragments were amplified from healthy fish spleen DNA,white spot syndrome baculoviruses(WSBV) DNA and pseudorabies virus(PRV) DNA.Detection limit of this method was 10^-7 ng positive plasmid DNA containing target sequence,equal to about 100 virions.In the infected experiment,first positive detection(1/4) appeared at Day 3 post-infection,all fish(4/4) tested positive at Day 7,however obvious symptoms were observed at Day 8,so LYCIV infection could be detected prior to the appearance of obvious symptoms.These results indicate that this PCR method could be used for early,rapid and specific detection of LYCIV infection.     

冲绳海槽中西部边缘区沉积物成因和沉积环境

本文根据１９９６年“中－法合作东海计划”在冲绳海槽中西部边缘区所取柱状样岩芯沉积物粒度分析资料,对该区沉积物特征进行研究。结果表明,火山沉积和富含生物的半远洋深海软泥沉积是本区的主要特征。以４８１ｃｍ为界,上、下两层沉积物截然不同,可能是沉积物的来源不同或者是水动力条件的差异。该区主要受火山活动的影响,其次是黑潮、大洋风暴     

Tropospheric lapse rate and its relation to surface temperature from reanalysis data

Estimates of the tropospheric lapse rate γ and analysis of its relation to the surface temperature T _s in the annual cycle and interannual variability have been made using the global monthly mean data of the NCEP/NCAR reanalysis (1948–2001). The tropospheric lapse rate γ is about 6.1 K/km in the Northern Hemisphere (NH) as a whole and over the ocean and about 6.2 K/km over the continents. The value of γ decreases from 6.5 K/km at low latitudes to 4.5 K/km at polar latitudes. The values of dγ/dT _s, the parameter of sensitivity of γ to the variation of T _s for the NH in the interannual variability, are found to be about 0.04 km^?1 (0.041 km^?1 for the NH as a whole, 0.042 km^?1 over the ocean, and 0.038 km^?1 over the continents). This corresponds to an increase in γ of approximately 0.7% when the surface temperature of the NH is increased by 1 K. Estimates of dγ/dT _s vary from about 0.05 km^?1 in the subtropics to 0.10 km^?1 at polar latitudes. When dγ/dT _s is positive, the surface and tropospheric warming means a temperature decrease above a certain critical level H _cr. The height of the level H _cr with constant temperature, which is defined by the inverse value (dγ/dT _s)^?1, is about 25 km for the NH as a whole, i.e., above the tropopause. In the subtropics, H _cr is about 20 km. At polar latitudes, H _cr decreases to about 10 km. Positive values of dγ/dT _s characterize a positive climatic feedback through the lapse rate and indicate a general decrease in the static stability of the troposphere during global warming. Along with a general tendency of γ to increase with rising T _s, there are regional regimes with the opposite tendency, mainly over the ocean. The negative correlation of γ with T _s is found over the oceanic tropics and midlatitudes, in particular, over the oceanic belt around Antarctica.     

A finite element model of interaction between viscous free surface waves and submerged cylinders

This paper describes the simulation of the flow of a viscous incompressible Newtonian liquid with a free surface. The Navier–Stokes equations are formulated using a streamline upwind Petrov–Galerkin scheme, and solved on a Q-tree-based finite element mesh that adapts to the moving free surface of the liquid. Special attention is given to fitting the mesh correctly to the free surface and solid wall boundaries. Fully non-linear free surface boundary conditions are implemented. Test cases include sloshing free surface motions in a rectangular tank and progressive waves over submerged cylinders.     

影响萼花臂尾轮虫种群增长的生态学因子的研究：温度和饵料密度的影响

本文研究了在5个温度梯度和4个饵料密度的20个试验组合下，萼化臂尾轮虫（Brachionuscalyciflorus）的种群增长规律。结果表明，该轮虫实验种群的最适温度范围是28—32℃，临界高温是36－40℃；最适饵料密度是45－65×106个／ml。这两个生态因子的最佳组合是温度32℃，饵料密度为65×106个／ml，此时，种群达最大密度3．4×103个／ml。     

一个湍能封闭模型对风暴潮海底底应力的应用研究

将一个三维湍能封闭模型应用于开阔海区的风暴潮，通过数值计算探讨了Ｔａｙｌｏｒ底摩擦二次率的拖曳系数随空间的分布及拖曳系数与水深、海底粗糙度、风向和风速等因素的关系。本文对底摩擦二次率的可靠性做了评价。     

大生活用海水生化处理技术研究进展

采用海水作为大生活用水最结解沿海城市淡水资源紧缺局面的有效途径之一。该技术得以推广应用的技术关键是大生活用海水的后处理技术，文章综述了大生活用海水的生化处理技术的研究现状，并提出了今后的研究方向。