Bioenhancement of cadmium transfer along a multi-level food chain

Previous studies have shown that metal partitioned to a subcellular compartment containing trophically available metal (TAM) is readily available to predators and may be enhanced by increased binding of metal to heat-stable proteins (HSP - e.g., metallothioneins). The aim of the current investigation was to determine the influence of TAM on the trophic transfer of Cd along an experimental, three-level food chain: Artemia franciscana (brine shrimp)-->Palaemonetes pugio (grass shrimp)-->Fundulus heteroclitus (mummichog). P. pugio were fed for 7 days on A. franciscana exposed to Cd in solution (including (109)Cd as radiotracer) and subjected to subcellular fractionation or fed to F. heteroclitus. An HSP-driven increase in the percentage of Cd associated with TAM (TAM-Cd%) in A. franciscana exposed to 1 muM Cd resulted in a bioenhancement (i.e., a greater than linear increase with respect to A. franciscana exposure) of Cd trophic transfer to P. pugio. Increased dietary Cd exposure did not affect TAM-Cd% in P. pugio nor trophic transfer to F. heteroclitus.     

Spectrophotometric pH measurement in estuaries using thymol blue and m-cresol purple

The conditional acid dissociation constants (pK_a′) of two sulfonephthalein dyes, thymol blue (TB) and m-cresol purple (mCP), were assessed throughout the estuarine salinity range (0<S<40) using a tris/tris–HCl buffer and spectrophotometric measurement. The salinity dependence of the pK_a′ of both dyes was fitted to the equations (25 °C, total proton pH scale, mol kg soln⁻¹):

The estimated accuracy of pH measurements using these calculated pK_a′ values is considered to be comparable to that possible with careful use of a glass electrode (±0.01 pH unit) but spectrophotometric measurements in an estuary have the significant advantage that it is not necessary to calibrate an electrode at different salinities. pH was measured in an estuary over a tidal cycle with a precision of ±0.0005 pH unit at high (S>30) salinity, and ±0.002 pH unit at low (S<5) salinity. The pH increased rapidly in the lower salinity ranges (0<S<15) but less rapidly at higher salinities.     

Response of the blue mussel Mytilus edulis L. following exposure to PAHs or contaminated sediment

The polycyclic aromatic hydrocarbons fluoranthene and benzo(a)pyrene significantly reduced the feeding rate of mussels. For both compounds the tissue concentration resulting in a 50% reduction of the clearance rate (TEC₅₀) was calculated. At high tissue concentrations both aromatic compounds reduced the tolerance of mussels to aerial exposure, whereas at low tissue concentrations an improved response was noticed. The activities of the antioxidant enzymes Superoxide dismutase (SOD) and catalase were elevated only at low tissue concentrations of fluoranthene and benzo(a)pyrene. At the highest measured tissue concentrations the activity of both enzymes was reduced, possibly due to a narcotic effect. The reproductive success rate of mussels appeared to be affected negatively by the investigated hydrocarbons. The results of a pilot experiment indicate that mussels can be used also for the testing of contaminated sediments.     

Calculation of the nonlinear energy transfer through the wave spectrum at the sea surface covered with broken ice

The nonlinear energy transfer through the wave spectrum is studied on the basis of the previously obtained explicit equation for matrix elements of a four-wave kinetic integral. The equation describes the evolution of a system of gravity waves at the surface of a sea of finite depth with a uniform distribution of broken ice over the sea surface. Particular attention is paid to the analytical part of the algorithm of the calculation of the kinetic integral. This part differs from the standard algorithm by a set of prominent features of the dispersion relation for wave oscillations in the ice-covered water. The kinetic integral for the system under consideration is calculated, and the results are compared with the results obtained for the ice-free water.     

Age of the western Black Sea basin according to an analysis of the anomalous magnetic field and geological data

Until recently, the ideas about the age of the Black Sea deep-water basin have been based on land geological observations in the coastal areas at the interaction periphery, underwater observations from manned submersibles, and on the data of seismic reflection and refraction studies and drilling. Formerly, the scarcity of the information led to a wide scattering of the age determinations: from the Jurassic to the Eocene. Recently, with the appearance of reliable geological and geophysical data, the range of the age estimates has been considerably reduced during the last few years, although there is no commonly accepted opinion on this issue. Therefore, the first attempt to determine the age of the Western Black Sea basin using an analysis of the anomalous magnetic field is of certain interest. The following results were obtained: the basin probably opened between 71.338 and 71.587 My B.P. (subchron C32n.1r). During the interval 68.737–71.071 My B.P. (subchron C31r), extinction of the spreading axes took place. Thus, the total duration of the Campanian-Maestrichtian phase of the opening was about 3 My (interval from 71.587 to 68.737 My B.P.). This result does not agree with the geological and geophysical data available to date. To solve this problem, collection of new geological data and further studies of the structure of the anomalous magnetic field are required.     

Phytoremediation of small-scale oil spills in fresh marsh environments: a mesocosm simulation

Research was conducted to assess the impact of oiling on fresh-marsh plant communities and to test the efficacy of techniques that may be used to enhance the bioremediation of crude oil spills in these environments while minimizing secondary anthropogenic impacts. To emulate field conditions, a mesocosm facility was used that houses 120 mesocosm vessels, each of 200-1 capacity. A five-way factorial treatment arrangement was used that included two substrates (inorganic, organic), two nutrient regimes (fertilized, not fertilized), two aeration levels (substrate aeration, no aeration), three oiling concentrations (0-, 5-, 10-1 m(-2) of South Louisiana Sweet Crude oil), and four vascular plant species (Alternanthera philoxeroides, Panicum hemitomon, Phragmites australis, Sagittaria lancifolia, and an unplanted control). Under the 5- and 10-1 m(-2) oiling concentrations, S. lancifolia displayed a short-term response of increased productivity, whereas P. hemitomon had the highest biomass production and photosynthetic rates at the end of the 18-month experiment. Overall plant growth and productivity, as well as oil degradation, were significantly higher in the inorganic substrate, indicating that biodegradation of oil spills in organic substrates may require a longer time period. Time-released fertilizer also stimulated plant productivity and resulted in higher soil respiratory quotients, suggestive of greater microbial activity, particularly in aerated mesocosms. The amount of oil remaining after 18 months was lowest in aerated and fertilized mesocosms containing either P. hemitomon or S. lancifolia and a substrate of low organic matter content.     

Sediment Deposition and Resuspension in Mouth Bar Area of the Yangtze Estuary

—A comprehensive analysis is conducted based on observations on topography.tidal current.salinity.suspended sediment and bed load during the years of 1982.1983.1988.1989.1996 and 1997 in theYangtze Estuary.Results show that the deformation of tidal waves is distinct and the sand carrying capaci-ty is large within the mouth bar due to strong tidal currents and large volume of incoming water and sedi-ments.Owing to both temporal and spatial variation of tidal current.deposition and erosion are extremelyactive.In general a change of up to 0.1 m of bottom sediments takes place during a tidal period.The maxi-mum siltation and erosion are around 0.2 m in a spring to neap tides cycle.The riverbed is silted duringflood when there is heavy sediment load.eroded during dry season when sediment load is low.The annualaverage depth of crosion and siltation on the riverbed is around 0.6 m.In particular cases.it may increaseto 1.4 m to 2.4 m at some locations.