Potential Use of Biomarkers in Zooplankton as Early Warning Signals of Ecotoxicological Risk in the Marine Food Chain

Abstract. Zooplankton is an essential component of the marine and brackish food chains. The ecotoxicological risk of zooplanktonic communities, estimated by the modern methodological approach of biomarkers, can be used as an early warning signal of ecosystem health. The aim of this project is to estimate the potential use of several biomarkers (esterases, mixed function oxidases, porphyrins) in zooplanktonic organisms. Studies were carried out with different zooplanktonic crustaceans: the copepods Acartia margalefi and Acartia latisetosa collected in Ganzirri Lake (Messina); the mysid Siriella clausi collected in Faro Lake (Messina); the mysids Diamysis bahirensis, Siriella armata and Mysidopsis gibbosa collected in Stagnone di Marsala (Palermo); the Antarctic euphausiids Euphausia crystallorophias and Euphausia superba; the am-phipod Streetsia challengeri and the euphausiid Meganycthiphanes norvegica collected after a shore-stranding along Messina's Ionian coast. Moreover, experiments were carried out with the benthic decapods Eriphia verrucosa and Pachygrapsus marmoratus from a rocky shore of Messina's Ionian coast. Acetylcholinesterase activity (AChE) was determined in homogenates of whole organisms. The key result of this project concerns the different AChE activity basal values of different crustacean species. Particular attention should be paid to the difference in basal activity found between the Antarctic and the Mediterranean species.     

Evaluation of ecotoxicological effects of endocrine disrupters during a four-year survey of the Mediterranean population of swordfish (Xiphias gladius)

In this project we investigated the ecotoxicological effects of endocrine disrupters in a four-year survey of the Mediterranean population of swordfish (Xiphias gladius). In the Mediterranean environment, top predators, such as swordfish, accumulate high concentrations of polyhalogenated aromatic hydrocarbons (PHAHs) and toxic metals, potentially incurring high toxicological risk. The effects of organochlorines and trace elements (Hg, Cd and Pb) in 192 swordfish specimens, caught in the Strait of Messina, Sicily, Italy, were investigated using vitellogenin (Vtg), zona radiata proteins (Zrp) and CYP1A (BPMO, EROD) activities. Vtg and Zrp were found to be dramatically induced in some adult male specimens, suggesting that this species is highly exposed to estrogens in the Mediterranean Sea. A role of organochlorines in this induction phenomenon is suggested by the statistically significant correlations between Zrp in plasma and PCB concentrations in muscle (p<0.032) and Vtg in plasma and PCB concentrations in liver (p<0.034) of male specimens. Levels of trace elements in liver were in the following ranges: Hg 1–22, Cd 1–28 and Pb 0–1.6 ppm d.w. These data indicate potential reproductive alterations in large pelagic fish and suggest the need for continuous monitoring to avoid reductions in the population of this fish species of high commercial and ecological interest.     

The use of a non-lethal tool for evaluating toxicological hazard of organochlorine contaminants in Mediterranean cetaceans: new data 10 years after the first paper published in MPB

In the Mediterranean Sea, top predators, and particularly cetacean odontocetes, accumulate high concentrations of organochlorine contaminants and toxic metals, incurring high toxicological risk. In this paper we investigate the use of the skin biopsies as a non-lethal tool for evaluating toxicological hazard of organochlorines in Mediterranean cetaceans, presenting new data 10 years after the paper published by Fossi and co-workers [Mar. Poll. Bull. 24 (9) (1992) 459] in which this new methodology was first presented. Some organochlorine compounds, now with worldwide distribution, are known as endocrine disrupting chemicals (EDCs). Here the unexplored hypothesis that Mediterranean cetaceans are potentially at risk due to organochlorines with endocrine disrupting capacity is investigated. High concentrations of DDT metabolites and PCB congeners (known as EDCs) were found in the different Mediterranean species (Stenella coeruleoalba, Delphinus delphis, Tursiops truncatus and Balaenoptera physalus). In this paper we also propose benzo(a)pyrene monooxygenase (BPMO) activity in marine mammal skin biopsies (non-lethal biomarker) as a potential indicator of exposure to organochlorines, with special reference to the compounds with endocrine disrupting capacity. A statistically significant correlation was found between BPMO activity and organochlorine levels (DDTs, pp(')DDT, op(')DDT, PCBs and PCB99) in skin biopsies of males of B. physalus. Moreover a statistical correlation was also found between BPMO activity and DDT levels in skin biopsies of the endangered Mediterranean population of D. delphis. These results suggest that BPMO induction may be an early sign of exposure to organochlorine EDCs and can be used for periodic monitoring of Mediterranean marine mammal toxicological status.     

Possibilities of soil spectroscopy for the classification of contaminated areas in river floodplains

During the past decades, large amounts of diffuse contaminated soil material have been deposited in the floodplains of the river Rhine in the Netherlands. The dynamic character of this river causes a large spatial variability in the contamination level of its floodplain soils. Characterisation of the spatial variability exclusively based on soil sampling and analysis is often insufficient and expensive. Hyperspectral images can provide additional spatial information for a proper characterisation of the contamination situation of river floodplains. This paper describes the possible application of soil spectroscopy to estimate metal concentration levels in river floodplains. Soil reflectance spectra in the visible-near infrared region (VNIR) were measured in the laboratory for soil samples taken from two river floodplains along the river Waal, the main tributary of the river Rhine in the Netherlands. A multivariate calibration procedure using partial least squares (PLS) regression was applied to establish a relationship between reflectance spectra in the visible-near infrared (VNIR) region and spectrally active soil characteristics (organic matter and clay content) that are intercorrelated with concentration levels of Cd and Zn. Results of the analysis of two river floodplains are summarised and the influence of scale-level and sub soil material on the prediction capability is discussed.     

A New Octahedrite from South Africa

Abstract Examination of an iron meteorite found in South Africa discloses it to be a coarse octahedrite containing 6.90 weight-percent nickel. The meteorite body contains several inclusions of troilite, or troilite plus graphite, and at least one of pure graphite veined with kamacite. Kamacite, cohenite, and phosphides are the other major constituents of the structure.     

Thermal models of flaring region based on observations by the SOLRAD 10 satellite

A number of solar X-ray bursts, observed with the SOLRAD 10 satellite on 1971 September 14, 17, November 28 and 1972 August 1, have been analysed. From the differential emission measure distribution deduced from the observations the temporal and spatial distribution in a flaring region has been evaluated and compared with models of instantaneous or continuous energy injection and conductive redistribution. Assuming a power law for the differential emission measure in dependence of electron temperature the ratios of the counting rates observed in different spectral bands have been compared with theoretical predictions so as to test the validity of this particular class of models.     

中国西部铂矿床类型与找矿方向探讨

中国铂矿资源严重不足,主要分布在中国西部,根据原生矿床特征可划分为4类：①硫化物型;②铬铁矿型;③辉石岩型;④层控型。寻找铂矿新类型和富铂(岩石)资源,应成为解决中国铂矿资源紧缺的重要任务。铂矿找矿方向：地台与地槽接合部位,特别是围绕着基性、超基性岩内外接触带,铂的预富集岩带与层控型铂矿带,另外还有砂铂型铂矿发育区有望扩大找铂前景。