Establishment and characterization of a testicular Sertoli cell line from olive flounder <Emphasis Type="Italic">Paralichthys olivaceus</Emphasis>

The culture of Sertoli cells has become an indispensable resource in studying spermatogenesis. A new Sertoli cell line (POSC) that consisted predominantly of fibroblast-like cells was derived from the testis of the olive flounder Paralichthys olivaceus and sub-cultured for 48 passages. Analysis of the mtDNA COI gene partial sequence confirmed that the cell line was from P. olivaceus. Cells were optimally maintained at 25°C in DMEM/F12 medium supplemented with fetal bovine serum, basic fibroblast growth factor, and epidermal growth factor. The growth curve of POSC showed a typical “S” shape. Chromosome analysis revealed that the cell line possessed the normal P. olivaceus diploid karyotype of 2n=48t. POSC expressed dmrt1 but not vasa, which was detected using RT-PCR and sequencing. Immunocytochemistry revealed that the cells exhibited the testicular Sertoli cell marker FasL. Therefore, POSC appeared to consist of testicular Sertoli cells. Bright fluorescent signals were observed after the cells were transfected with pEGFP-N3 plasmid, with the transfection efficiency reaching 10%. This research not only offers an ideal model for further gene expression and regulation studies on P. olivaceus, but also serves as valuable material in studying fish spermatogenesis, Sertoli cell-germ cell interactions, and the mechanism of growth and development of testis.     

华南埃迪卡拉(震旦)系顶部地层划分及与寒武系界线FAD分子的选择

"雪球事件"以来,早期生命呈现多层次、大规模的加速演化,到寒武纪前夕,生命大爆发达到高潮;在生命剧烈演替的同时,地球环境标志也发生着响应,碳同位素剧烈漂移,并且与各个生命演化节点相对应。但是在划分最重要的地质界线,埃迪卡拉(震旦)系——寒武系界线及界线层型和点位("金钉子",GSSP)确立的问题上迄今仍存在着较大的分歧,以遗迹化石Treptichnus pedum作为该GSSP的标志化石,因其跨系、跨阶产出,生物延限长,因而在全球的生物地层划分对比中(特别在西伯利亚和中国华南地区)一直质疑声不断,而且以遗迹化石作为标志化石本身也具有一定的缺陷。中国华南地区两类古老的宏体化石——矿化管状的Cloudina和条带状的Shaanxilithes在全球范围内可以很好的对比,并且这两种实体化石的出现都对应着碳同位素的明显正漂移。本文通过研究华南震旦系顶部化石带的地层分布和地理分布,综合分析了其时空分布特点和系统演化意义,深入探讨了Cloudina和Shaanxilithes作为寒武系底界标志化石所具备的潜力,对埃迪卡拉(震旦)纪—寒武纪地层界线划分和层型选择提出了更为理想的标准。     

Theoretical calculation of equilibrium Mg isotope fractionation between silicate melt and its vapor

Isotope fractionation during the evaporation of silicate melt and condensation of vapor has been widely used to explain various isotope signals observed in lunar soils, cosmic spherules, calcium–aluminum-rich inclusions, and bulk compositions of planetary materials. During evaporation and condensation, the equilibrium isotope fractionation factor (α) between high-temperature silicate melt and vapor is a fundamental parameter that can constrain the melt’s isotopic compositions. However, equilibrium α is difficult to calibrate experimentally. Here we used Mg as an example and calculated equilibrium Mg isotope fractionation in MgSiO₃ and Mg₂SiO₄ melt–vapor systems based on first-principles molecular dynamics and the high-temperature approximation of the Bigeleisen–Mayer equation. We found that, at 2500 K, δ²⁵Mg values in the MgSiO₃ and Mg₂SiO₄ melts were 0.141?±?0.004 and 0.143?±?0.003‰ more positive than in their respective vapors. The corresponding δ²⁶Mg values were 0.270?±?0.008 and 0.274?±?0.006‰ more positive than in vapors, respectively. The general \(\alpha - T\) equations describing the equilibrium Mg α in MgSiO₃ and Mg₂SiO₄ melt–vapor systems were: \(\alpha_{{{\text{Mg}}\left( {\text{l}} \right) - {\text{Mg}}\left( {\text{g}} \right)}} = 1 + \frac{{5.264 \times 10^{5} }}{{T^{2} }}\left( {\frac{1}{m} - \frac{1}{{m^{\prime}}}} \right)\) and \(\alpha_{{{\text{Mg}}\left( {\text{l}} \right) - {\text{Mg}}\left( {\text{g}} \right)}} = 1 + \frac{{5.340 \times 10^{5} }}{{T^{2} }}\left( {\frac{1}{m} - \frac{1}{{m^{\prime}}}} \right)\), respectively, where m is the mass of light isotope ²⁴Mg and m′ is the mass of the heavier isotope, ²⁵Mg or ²⁶Mg. These results offer a necessary parameter for mechanistic understanding of Mg isotope fractionation during evaporation and condensation that commonly occurs during the early stages of planetary formation and evolution.     

Elemental characteristics of lacustrine oil shale and its controlling factors of palaeo-sedimentary environment on oil yield: a case from Chang 7 oil layer of Triassic Yanchang Formation in southern Ordos Basin

As an important unconventional resource, oil shale has received widespread attention. The oil shale of the Chang 7 oil layer from Triassic Yanchang Formation in Ordos Basin represents the typical lacustrine oil shale in China. Based on analyzing trace elements and oil yield from boreholes samples, characteristics and paleo-sedimentary environments of oil shale and relationship between paleo-sedimentary environment and oil yield were studied. With favorable quality, oil yield of oil shale varies from 1.4% to 9.1%. Geochemical data indicate that the paleo-redox condition of oil shale’s reducing condition from analyses of V/Cr, V/(V + Ni), U/Th, δU, and authigenic uranium. Equivalent Boron, Sp, and Sr/Ba illustrate that paleosalinity of oil shale is dominated by fresh water. The paleoclimate of oil shale is warm and humid by calculating the chemical index of alteration and Sr/Cu. Fe/Ti and (Fe + Mn)/Ti all explain that there were hot water activities during the sedimentary period of oil shale. In terms of Zr/Rb, paleohydrodynamics of oil shale is weak. By means of Co abundance and U/Th, paleo-water-depth of oil shale is from 17.30 to 157.26 m, reflecting sedimentary environment which is mainly in semi deep–deep lake facies. Correlation analyses between oil yield and six paleoenvironmental factors show that the oil yield of oil shale is mainly controlled by paleo-redox conditions, paleoclimate, hot water activities, and depth of water. Paleosalinity and paleohydrodynamics have an inconspicuous influence on oil yield.     

乌尉高速公路边坡稳定性综合评价

针对乌尉(乌鲁木齐至尉犁)高速公路地处高寒、高海拔、高地震烈度区,地质环境条件复杂的特点,提出一种基于多判据的边坡稳定性模糊综合评价方法。首先,分析研究三高地区公路沿线工程地质条件、边坡岩体结构特征及其变形破坏模式。在此基础上,以TSMR评价体系为框架,考虑地震荷载对边坡稳定性的影响,引入地震作用影响系数β,建立了适用于“三高”地区公路边坡岩体质量分级体系THRSMR;基于边坡可靠性分析理论,归纳总结Monte Carlo法的操作步骤,并借助GEO-SLOPE软件,给出各研究边坡的可靠度指标与失效概率;最后,将定性评价、极限平衡分析、可靠度分析和数值计算的结果分别作为基础指标,采用模糊综合评价方法对每一边坡的边坡稳定性,给出进一步量化结论。其结果对类似地区高速公路边坡稳定性评价具有一定的参考价值。     

Study on the deformation and failure modes of filling slope in loess filling engineering: a case study at a loess mountain airport

Lvliang airport is a typical loess filling engineering located in 20.5 km north of Lvliang City in Shanxi Province, China. By the end of March 2012, 14 fissures extending more than 7.5 m were observed in a loess-filled slope, of which the longest fissure is up to 82 m. Field monitoring and laboratory tests have been performed to investigate the slope failure modes. The test program includes wetting tests on unsaturated compacted samples and stress path tests on saturated samples. Field monitoring and observations show that differential settlement caused by non-homogeneity in compacted loess density might lead to the formation of fissures in the loess-filled slope. It was founded that the wetting deformation contributed to the development of differential settlement. Fissures are the essential factor for the loess-filled slope failure. Four deformation stages exhibit in the loess-filled slope prior final failure including development of the fissures, softening of the compacted loess, creeping of the slope leading edge and fissuring of the trailing edge and formation of the through-sliding surface. Development of the sliding surface mainly includes upward and downward expansion of the fissures. Upward expansion is a wetting failure process in loading condition, while downward expansion is a load-off wetting process. In addition, development of the sliding surface is accelerated by softening of the compacted soils as a result of water infiltration. Therefore, the key for taking countermeasures against filling landslides is to monitor and control the development of differential settlement and fissures in the slope shoulders. Digging out and extra-filling the fissures are an effective way for preventing these landslides.     

Transport behavior of nZnO in geosynthetic clay liner used in municipal solid waste landfills under temperature effect

The transport process of nZnO in geosynthetic clay liner (GCL), an anti-seepage material used in municipal solid waste (MSW) landfills, under different seepage temperature conditions was conducted. The transport behavior of nZnO in GCL was analyzed. Results showed that the retardation performance of current GCL used in MSW landfills anti-seepage system against nZnO pollutants was poor. nZnO successfully permeated the GCL and entered external soil–groundwater environment, posing health threats to the life of organisms. Although seepage temperature exerted a small effect on nZnO suspension leakage volume, change in seepage temperature affects the mass of transported nZnO in GCL by redispersion of nZnO in suspension. As the seepage temperature increases, the mass of nZnO that permeated the GCL increases, reaching a maximum at 50 °C, and then decreases.     

Heavy metals status,transport mechanisms,sources, and factors affecting their mobility in Chinese agricultural soils

Heavy metals are the focus of much interest because of their environmental persistence, toxicity, and mobility in soils. Some of the Chinese soils have been contaminated with heavy metals due to expansion of mining industries, pesticides usage, and other anthropological activities, which made the agro-ecosystem to become contaminated. The objective of this review is to illuminate the current pollution status, sources of heavy metals, transport mechanisms, and the factors affecting their mobility in Chinese soils. The additional studies in the future will present on source identification and the heavy metal transport characteristics in soil.     

An experimental investigation into the compaction characteristic of granulated gangue backfilling materials modified with binders

Solid backfill mining has apparent technical advantages in extracting coal resource under sensitive surface structures such as buildings and roads; meanwhile, as an effective method of disposing mining wastes, it works well in solving some environmental problems caused by mining activities. In addition, the controlling effect of solid backfill mining is directly related to the compaction characteristic of backfilling materials. The present study aims to modify the backfilling materials by assessing the effect of binders (cement, fly ash, and lime) on the compaction characteristic of granulated gangue backfilling materials. The compaction test was performed with rock mechanic test system equipped with a self-made circular cylinder apparatus. From the results obtained, cement is not the suitable binder for modifying the gangues backfilling materials, while fly ash or lime, when the dosage is up to 20 wt%, is beneficial to the compaction characteristic of backfilling materials. The relationship between strain behavior and micro-structure of backfilling materials was investigated by SEM and the effect of fly ash or lime on the strain behavior of backfilling materials could be associated with its cementation and gap-filling effect.