Comparison of Nutrient and Microbial Dynamics over a Seasonal Cycle in a Mid-Atlantic Coastal Lagoon Prone to <Emphasis Type="Italic">Aureococcus anophagefferens</Emphasis> (Brown Tide) Blooms

To determine the roles of dissolved organic matter in the onset, duration, and decline of blooms of the “brown tide” pelagophyte, Aureococcus anophagefferens, nutrient and microbial dynamics, heterotrophic and autotrophic carbon (C) and nitrogen (N) uptake, and peptide hydrolysis were compared in natural populations: (1) seasonally, among physically similar sites in a mid-Atlantic coastal lagoon, Chincoteague Bay, (2) at an individual site as a bloom initiated, developed, and declined, and (3) in whole versus size-fractionated water. Throughout the year, urea was the dominant form of N taken up at both bloom and nonbloom sites. C acquisition in the A. anophagefferens (1.2–5.0 μm) size fraction was dominated by bicarbonate uptake during bloom initiation but organic C compounds were taken up later during and after the bloom. Bacterial productivity was enhanced during and just after the bloom and bacterial abundance was four times higher at the bloom versus nonbloom site.     

Predicting high-frequency variation in stream solute concentrations with water quality sensors and machine learning

Stream solute monitoring has produced many insights into ecosystem and Earth system functions. Although new sensors have provided novel information about the fine-scale temporal variation of some stream water solutes, we lack adequate sensor technology to gain the same insights for many other solutes. We used two machine learning algorithms – Support Vector Machine and Random Forest – to predict concentrations at 15-min resolution for 10 solutes, of which eight lack specific sensors. The algorithms were trained with data from intensive stream sensing and manual stream sampling (weekly) for four full years in a hydrologic reference stream within the Hubbard Brook Experimental Forest in New Hampshire, USA. The Random Forest algorithm was slightly better at predicting solute concentrations than the Support Vector Machine algorithm (Nash-Sutcliffe efficiencies ranged from 0.35 to 0.78 for Random Forest compared to 0.29 to 0.79 for Support Vector Machine). Solute predictions were most sensitive to the removal of fluorescent dissolved organic matter, pH and specific conductance as independent variables for both algorithms, and least sensitive to dissolved oxygen and turbidity. The predicted concentrations of calcium and monomeric aluminium were used to estimate catchment solute yield, which changed most dramatically for aluminium because it concentrates with stream discharge. These results show great promise for using a combined approach of stream sensing and intensive stream discrete sampling to build information about the high-frequency variation of solutes for which an appropriate sensor or proxy is not available.     

Absence of seasonal patterns in MBT-CBT indices in mid-latitude soils

The degree of methylation and cyclization of bacteria-derived branched glycerol dialkyl glycerol tetraether (GDGT) membrane lipids in soils depends on temperature and soil pH. Expressed in the methylation index of branched tetraethers (MBT) and cyclization ratio of branched tetraethers (CBT), these relationships are used to reconstruct past annual mean air temperature (MAT) based on the distribution of branched GDGTs in ancient sediments; the MBT-CBT proxy. Although it was shown that the best correlation of this proxy is with annual MAT, it remains unknown whether a seasonal bias in temperature reconstructions could occur, such as towards a seasonal period of ‘optimal growth’ of the, as yet, unidentified soil bacteria which produce branched GDGTs. To investigate this possibility, soils were sampled from eight different plots in the USA (Minnesota and Ohio), The Netherlands (Texel) and the UK (Devon) in time series over 1 year and analyzed for their branched GDGT content. Further analyses of the branched GDGTs present as core lipids (CLs; the presumed fossil pool) and intact polar lipids (IPLs; the presumed extant pool) were undertaken for two of the investigated soil plots. The amount of IPL-derived branched GDGTs is low relative to the branched GDGT CLs, i.e. only 6-9% of the total branched GDGT pool. In all soils, no clear change was apparent in the distribution of branched GDGT lipids (either core or IPL-derived) with seasonal temperature change; the MBT-CBT temperature proxy gave similar temperature estimates year-round, which generally matched the mean annual soil temperature. In addition to a lack of coherent changes in relative distributions, concentrations of the branched GDGTs did not show clear changes over the seasons. For IPL-derived GDGTs these results suggest that their turnover time in soils is in the order of 1 year or more. Thus, our study does not provide evidence for seasonal effects on the distribution of branched GDGTs in soils, at least at mid-latitudes, and therefore, no direct evidence for a bias of MBT-CBT reconstructed temperatures towards a certain season of optimal growth of the source bacteria. If, however, there is a slight seasonal preference of branched GDGT production, which can easily be obscured by natural variability due to the heterogeneity of soils, then a seasonal bias may potentially still develop over time due to the long turnover time of branched GDGTs.     

The Bioavailability of Effluent-derived Organic Nitrogen along an Estuarine Salinity Gradient

Total maximum daily loads for nitrogen (N) are currently being established for the Chesapeake Bay watershed. While we know inorganic N is bioavailable in the environment and therefore its input contributes to cultural eutrophication, the bioavailability of organic N is unclear. Using bioassay experiments, we examined the impact of effluent-derived organic nitrogen (EON) from wastewater treatment plants on natural water samples collected along an estuarine/salinity gradient within the lower Chesapeake Bay watershed. All of the inorganic N and between 31% and 96% of the EON was removed during biotic bioassays within the first 2 days. Further, there was substantial abiotic reactivity of effluent N when it was added to natural water samples. Results demonstrate that organic and inorganic N in effluent is removed to support the growth of microbial communities. These are the first results aimed at assessing the reactivity of EON in natural waters along an estuarine/salinity gradient.     

Compositional zoning of calcite in a high-pressure metamorphic calc-schist: clues to heterogeneous grain-scale fluid distribution during exhumation

Calcite in former aragonite–dolomite-bearing calc-schists from the ultrahigh-pressure metamorphic (UHPM) oceanic complex at Lago di Cignana, Valtournanche, Italy, preserved different kinds of zoning patterns at calcite grain and phase boundaries. These patterns are interpreted in terms of lattice diffusion and interfacial mass transport linked with a heterogeneous distribution of fluid and its response to a changing state of stress. The succession of events that occurred during exhumation is as follows: As the rocks entered the calcite stability field at T=530–550 °C, P ca. 1.2 GPa, aragonite occurring in the matrix and as inclusions in poikilitic garnet was completely transformed to calcite. Combined evidence from microstructures and digital element distribution maps (Mn-, Mg-, Fe- and Ca–Kα radiation intensity patterns) indicates that transformation rates have been much higher than rates of compositional equilibration of calcite (involving resorption of dolomite and grain boundary transport of Mg, Fe and Ca). This rendered the phase transformation an isochemical process. During subsequent cooling to T ca. 490 °C (where lattice diffusion effectively closed), grains of matrix calcite have developed diffusion-zoned rims, a few hundred micrometres thick, with Mg and Fe increasing and Ca decreasing towards the phase boundary. Composition profiles across concentrically zoned, large grains in geometrically simple surroundings can be successfully modelled with an error function describing diffusion into a semi-infinite medium from a source of constant composition. The diffusion rims in matrix calcite are continuous with quartz, phengite, paragonite and dolomite in the matrix. This points to an effective mass transport on phase boundaries over a distance of several hundred micrometres, if matrix dolomite has supplied the Mg and Fe needed for incorporation in calcite. In contrast, diffusion rims are lacking at calcite–calcite and most calcite–garnet boundaries, implying that only very minor mass transport has occurred on these interfaces over the same T–t interval. From available grain boundary diffusion data and experimentally determined fluid–solid grain boundary structures, inferred large differences in transport rates can be best explained by the discontinuous distribution of aqueous fluid along grain/phase boundaries. Observed patterns of diffusion zoning indicate that fluid was distributed not only along grain-edge channels, but spread out along most calcite–white mica and calcite–quartz two-grain junctions. On the other hand, the inferred non-wetting of calcite grain boundaries in carbonate-rich domains is compatible with fluid–calcite–calcite dihedral angles >60° determined by Holness and Graham (1995) for a wide range of fluid compositions under the P–T conditions of interest. Whereas differential stress has been very low at the stage of diffusion zoning (T > 490 °C), it increased as the rocks were cooling below 440 °C (at 0.3–0.5 GPa). Dislocation creep and the concomitant increase of strain energy in matrix calcite induced migration recrystallisation of high-angle grain boundaries. For that stage, the compositional microstructure of recrystallised calcite grain boundary domains indicates significant mass transport along calcite two-grain junctions, which at the established low temperatures is likely to have been accomplished by ionic diffusion within a hydrous grain boundary fluid film (“dynamic wetting” of migrating grain boundaries). Received: 10 January 2000 / Accepted: 10 April 2000     

A key-diagram for the optical determination of platinum-group minerals

Summary A key-diagram for optical routine investigation of platinum-group minerals has, for the first time, been developed on the basis of the most recent reflectance and microhardness data. The diagram contains 65 minerals, which occupy defined fields. The dominating platinum metal present in the respective minerals has been characterized by giving eachPGE a special colour. In addition, cubic (isotropic) minerals can be distinguished. The most important aspects of spectral reflectance have also been summarized.

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Ein Diagramm für die optische Bestimmung von Mineralen der Platingruppe

Zusammenfassung Ein Diagramm für die optische Routineuntersuchung von Mineralen der Platingruppe wurde erstmals auf Grund der neuesten Reflexions-und Mikrohärtedaten entwickelt. Das Diagramm enthält 65 Minerale, die imR-VHN-Feld definierte Bereiche besetzen, und je nach dem vorherrschenden Platinmetall durch bestimmte Farben charakterisiert sind. Zusätzlich werden auch kubische (isotrope) Minerale graphisch gekennzeichnet. Wichtige Aspekte der spektralen Reflexion sind ebenfalls dargestellt.

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With 1 Figure and 1 pull-out in colour     

Chronology of the Last Climatic Cycle (Upper Pleistocene) of the Surduk loess sequence, Vojvodina, Serbia

The Surduk loess section in Serbia provides a 20 m thick pedosedimentary record of the last interglacial–glacial climatic cycle (Upper Pleistocene). Based on optical dating, a chronostratigraphy could be established for the last climatic cycle, yielding the first numerical ages of a loess record from the middle Danube basin. Infrared-stimulated luminescence (IR-OSL) dating has been applied to the polymineral silt fraction using a multiple aliquot additive-dose protocol to determine the equivalent dose ( D _E ). Within error limits, all age estimates are in stratigraphic order. Owing to the application of shine-plateau tests, the samples showed no evidence of insufficient bleaching. The Surduk loess section comprises three major periods of soil formation. Based on the IR-OSL chronostratigraphy, the lowermost pedocomplex is attributed to the Last Interglacial and to the Early Glacial (Marine Isotope Stage – MIS 5e to 5a). The middle part of the section exhibits a succession of weakly developed brown soils and a humic horizon, named 'Surduk soil', formed during MIS 3. On top of the section, recent soil formation is related to the Holocene. Thick loess deposits are preserved between these palaeosols and are attributed to the Lower and Upper Pleniglacials (MIS 4 and 2), respectively. Estimated mean sedimentation rates are 0.1–0.2 mm/yr for the last glacial cycle, with a strong increase to 0.6 mm/yr with onset of the Pleniglacial.     

Blooms of Dinoflagellate Mixotrophs in a Lower Chesapeake Bay Tributary: Carbon and Nitrogen Uptake over Diurnal,Seasonal, and Interannual Timescales

A multi-year study was conducted in the eutrophic Lafayette River, a sub-tributary of the lower Chesapeake Bay during which uptake of inorganic and organic nitrogen (N) and C compounds was measured during multiple seasons and years when different dinoflagellate species were dominant. Seasonal dinoflagellate blooms included a variety of mixotrophic dinoflagellates including Heterocapsa triquetra in the late winter, Prorocentrum minimum in the spring, Akashiwo sanguinea in the early summer, and Scrippsiella trochoidea and Cochlodinium polykrikoides in late summer and fall. Results showed that no single N source fueled algal growth, rather rates of N and C uptake varied on seasonal and diurnal timescales, and within blooms as they initiated and developed. Rates of photosynthetic C uptake were low yielding low assimilation numbers during much of the study period and the ability to assimilate dissolved organic carbon augmented photosynthetic C uptake during bloom and non-bloom periods. The ability to use dissolved organic C during the day and night may allow mixotrophic bloom organisms a competitive advantage over co-occurring phytoplankton that are restricted to photoautotrophic growth, obtaining N and C during the day and in well-lit surface waters.     

Hollandite–strontiomelane solid solutions coexisting with kanonaite and braunite in late quartz veins of the Stavelot Massif, Ardennes, Belgium

In a newly found type of quartz vein cross-cutting the famous "viridine"-bearing phyllites at Le Coreux, hollandite, ideally BaMn₈O₁₆, was discovered for the first time at this locality and in Belgium. Because the crystals contain up to 60 mol% of the Sr end member, this is also the second occurrence of strontiomelane. The coexisting "viridine" (= kanonaite) contains the highest amount (88 mol%) of the ideal end member MnAlSiO₅ ever found worldwide. The hollandite-type minerals are intimately intergrown with braunite containing appreciable Ca and Mg. Ba-bearing muscovite, Fe-poor excess-Al clinochlore (not quite trioctahedral), and albite are the remaining accessory minerals in the dominant quartz matrix. Microprobe analyses of all phases show rather extreme element fractionations: nearly all K is located in muscovite and none in the hollandite phase despite the existence of the end member KMn₈O₁₆ (cryptomelane). Similarly, nearly all Na is in albite and not in hollandite (no NaMn₈O₁₆=manjiroite component). Nearly all Mn resides in the two oxide phases and in kanonaite. Mg is strongly fractionated into chlorite. The small amounts of Fe and Ti present are predominantly partitioned into the hollandite phase, which also accommodates most of the Ba and Sr. Indeed, the hollandite phase is stabilized by the latter two elements relative to other Mn oxides. Kanonaite is stabilized by Al. Although no requisite sites are available in its crystal structure, braunite always contains small amounts of Ba and Sr. However, the Sr/(Sr+Ba) ratios in braunite are spurious and unrelated to those of the directly adjoining hollandite phases The conditions of formation of these veins may be well below 300 °C at low pressures (1-2 kbar), in agreement with the experimental results that the maximum Mn contents in kanonaite increase with falling temperatures.