秋茄和榕树叶片中正构烷烃分布和单体化合物δ13C值及其光合作用

对深圳福田、阳江、海南新竹红树林秋茄Kandelia candel及海口榕树Ficusm icrocarpa叶中类脂物进行萃取,分离出正构烷烃,通过气相色谱(GC)及色谱-质谱(GC MS)分析,测出了正构烷烃的碳数分布和相对含量.气相色谱-稳定同位素比质谱仪(GC-IRMS)测定了正构烷烃单体化合物的稳定同位素组成δ13C.秋茄叶和榕树叶正构烷烃的碳数分布均为C17C33,主要集中在C23-C31(＞90%).化合物含量最高为C27的有深圳福田秋茄、海南新竹秋茄、海口榕树,而阳江秋茄为C25.单体化合物的稳定同位素δ13C深圳福田秋茄为-31.42‰-34.48‰,阳江秋茄为-31.69‰-33.62‰,海南新竹秋茄为-30.30‰-33.04‰,海口榕树为-29.92‰33.56‰;平均值分别为-32.95‰、32.67‰、31.67‰、-31.74‰.结果表明,秋茄和陆地植物榕树的光合作用都是通过C3途径,是典型C3植物.     

Dinoflagellate cysts in recent marine sediment from Guangxi, China

Total of 33 species of dinoflagellate cysts were discovered from surface sediment in the searegion of Guangxi, among them 12 cyst types (Diplopsalopsis sp.1, D. sp. 2, D. sp. 3, Cochlodinium sp., Protoperidinium sp. 1, P. sp. 2, P. compressum , Scrippsiella sp. 1, S. sp. 2, Alexandrium sp. 1, A. sp. 2, A. sp. 3) were first reported from the South China Sea. And one cyst type (Cochlodinium sp.) was first reported in the world. Scrippsiella trochoidea is the dominant species in this area, accounting for 45% of all the cysts. There are 2 cysts of toxic dinoflagellate (Alexandrium tamarensis and Gymnodinium catenatum). But there is no relationship between cyst number and grain size distribution.     

褐藻胶裂解酶产生菌Vibro sp.QY102的发酵条件优化

对海洋来源的Vibro sp.QY102的产褐藻胶裂解酶的发酵条件进行研究。结果表明,该菌株最适液体培养基成分为(w/v):0.5%褐藻酸钠;0.4%蛋白胨;0.3%KH2PO4;0.7%K2HPO4.3H2O;2%NaCl;0.01%MgSO4.7H2O,pH=6.0。按3%的接种量接入培养基,30℃150 r/min振荡培养120 h,产酶达到10.2 U/mL,为优化前的4.5倍。Mg2 是该菌株产酶所必需的,这在其他褐藻胶裂解酶生产菌株中未见报道。该菌株产酶发酵条件的研究,为褐藻胶裂解酶的大规模制备及应用奠定了基础。     

不同氮源对微小亚历山大藻生长和毒素产生的影响

通过尿素、氯化铵、酵母浸出粉和硝酸钠等氮源对微小亚历山大藻(Alexandrium minutum)生长及毒素产生的影响研究,分析了微小亚历山大藻对不同氮源利用状况的差异.结果表明,在氮饥饿条件下,加入硝酸钠和酵母浸出粉能显著促进微小亚历山大藻的生长;高浓度的氯化铵在加入后对微小亚历山大藻有一定的毒性效应,表现为生长停滞,但毒性效应在5 d后消失,并得到与添加硝酸钠及酵母浸出粉相似的增长速率0.21 d-1;添加尿素对微小亚历山大藻的生长没有显著促进作用.在四种氮源中,尿素对微小亚历山大藻毒素产生的刺激作用也最弱,在稳定期每个细胞藻细胞毒素含量维持在6.00~8.00 fmol;添加硝酸钠、氯化铵和酵母浸出粉的藻细胞在稳定期毒素含量分别达到11.85,12.86和14.64 fmol.硝酸钠和氯化铵刺激藻毒素产生的效果比酵母浸出粉更为直接.四种含氮营养盐对微小亚历山大藻毒素组成的影响都很小.     

Genetic relationship between Neobenedenia girellae and N. melleni inferred from 28S rRNA sequences

The fragments of 350 bp in 28S rRNA from the closely related monogenea of trematoda, Neobenedenia girellae and N. melleni are obtained by polymerase chain reaction (PCR) amplified using a couple of special primers and then sequenced. The results show that the comparison of 28S rRNA sequences, with only a base varying in 337 bp accounting for 0.3% genetic difference, from the relative species N. girellae and N. melleni parasitized on the different fishes in different farms displays that they possess a very high genetic similarity of 99.7%, higher than that of 99.41% for the single species N. melleni sampled in different areas, and the intraspecific divergence of N.melleni is 0.59%. Meanwhile, the interspecific differences between the two Neobenedenia and three Benedenia (i.e., B. lutjani, B. rohdei and B. seriolae) range from 2.08% tol 1.73%. In addition, UPGMA and MP molecular phylogenetic trees are constructed and proved to be consistent with each other. Though the morphological characteristics and the results of genetic diversity for the two Neobenedenia show a high similarity, whether they belong to a single species or not are still undefined, and the more genes of them should be further investigated, in combination with the systematical and detailed morphological study.     

南海表层沉积物中钙质超微化石分布特征

为系统描述钙质超微化石在南海表层沉积中的分布特征,对遍布南海的175个样品进行了实验分析。发现不同地区钙质超微化石绝对丰度相差很大,从0—3.8×1010个.g-1不等。平面上将钙质超微化石丰度分为3个区。共鉴定出钙质超微化石21属28种,以Emiliania huxleyi、Florisphaera profunda和Gephyrocapsa oceanica为优势种,其中Florisphaera profunda占据绝对优势。南海钙质超微化石分布具有两个明显特征:一是14°N线南北两边钙质超微化石的分布存在差异;二是南海钙质超微化石丰度以南沙群岛和西沙群岛两片海域为最高,并有东北-西南走向的分布趋势。对影响钙质超微化石分布的水深、上升流与营养盐、陆源物质稀释作用、碳酸盐溶解作用等因素作了讨论,并根据钙质超微化石随水深的变化推测南海碳酸盐补偿深度应在4 000m左右。     

胶州湾泛亚码头疏浚方案优选及悬浮物影响预测

采用有限元分步杂交方法,在已建潮流场的基础上,建立了胶州湾疏浚物悬浮泥沙的二维输运-扩散模型,并应用于胶州湾前湾港区泛亚码头工程疏浚区的疏浚物输运扩散的数值模拟预测。根据预测结果分析了泛亚码头疏浚区施工期间悬浮泥沙对附近海域水环境和生态环境的影响,并对不同的疏浚方案进行了优选。     

主动式声纳列阵拖曳系统姿态数值计算

主动式声纳列阵拖曳系统是用于探测潜艇的新型声纳系统，为了准确探测潜艇的位置，必须首先预报声纳列阵的瓷态，本文通过对其三维力学模型的分析，得到该系统的运动微分方程，其中缆索的力学方程是基于Ablow和Milinazzo的模型，而对于拖体则运用六自由度空间运动方程模拟，结合边界条件，用有限差分法求解，通过对拖船的不同运动状态如匀速，变速和回转的计算，证明本文的方法对于预报声纳列阵的姿态是有效的。     

The sudden disappearance of a dark filament observed on October 26, 1989

We present H monochromatic and spectroscopic observations of the sudden disappearance of a dark filament located near the center of the solar disk on October 26, 1989. The event was not associated with the flare activity. The dark filament first disintegrated into two loop-like components, and then each component successively showed ascending motion with a velocity greater than 30 km s^–1. Comparison of the H pictures taken before and after the start of this event suggests that the dark filament was originally composed of two magnetic flux loops.     

思林水电站通航建筑物高边坡稳定分析和评价

应用ANSYS结构分析软件,模拟了思林水电站通航建筑物高边坡的地形、地质条件以及开挖的过程,建立边坡开挖的有限元计算分析模型,分析边坡在自然状态、分级开挖以及锚杆支护加固等工况下的应力、变形及其整体稳定性.定义单元的局部安全系数,得到了边坡开挖加固后稳定安全系数的分布.根据计算成果,指出现设计加固措施可以保证边坡稳定,无需增加其他工程措施.