Crystal Structure and Geometry-Optimization Study of N-(2-hydroxy-1-naphthaldene)-4-aminoantipyrme

N-(2-hydroxy-1-naphthaldene)-4-aminoantipyrine has been synthesized. The structure is determined by X-ray diffraction method and elemental analysis. The crystal system belongs to orthorhombic space group P(2)2(1)2(1). The geometry has been ob-tained from the density functional theory (DFT) method and the B3LYP method employing the 6-31G* basis sets. The calculated results propose that the latter is close to the experimental data. The structural parameters from the theory are close to those of the crystal and the calculated total energy of coordination is -31677.172 eV. The energy of HOMO and LUMO and the energy gap are 5.179 eV, -1.603 eV and 3.577 eV, respectively.     

基于近景摄影测量法计算掘进隧洞中切穿顶拱的裂隙面产状

基于近景摄影测量原理,利用掘进隧洞掌子面上裂隙信息,提出了确定裂隙面产状的方法,并通过将被裂隙所切穿的顶拱曲面沿某一方向拉伸的”曲化平”方法,将弧形线转化成直线,然后应用矢量叉乘确定裂隙面产状,实现了对切穿顶拱的裂隙面产状的计算。最后,通过算例验证了该方法的可行性。     

传统海图和电子海图一体化数据模型的构建与应用研究

针对国内在一定时期内既要生产传统纸质海图保证传统导航,又要生产电子海图满足电子导航的需求,构建了一个能够包容S-57电子海图和传统海图信息全集的一体化海图数据模型,基于此模型分析了两种数据到一体化数据模型的转换机制,并应用此模型开发原型系统,实现了二者之间的一体化组织和切换显示,为探索传统海图和电子海图的数据融合提供了思路和参考。     

复合加载条件下吸力式沉箱基础承载特性数值分析

吸力式沉箱基础的承载特性是海洋工程结构设施建造与设计中的一个关键问题。这种新型的深水海洋基础型式,通常承受竖向上拔荷载与水平荷载的共同作用,其工作性能与设计理论远远不能满足工程实践的需要。本文采用有限元分析方法对吸力式沉箱基础的极限承载特性进行数值计算。以大型通用有限元分析软件ABAQUS为平台,通过二次开发,数值实现了Swipe试验加载方法和固定位移比分析方法,针对不同的沉箱长径比、土的强度折减系数,探讨了沉箱基础在垂直上拔荷载和水平荷载单调联合作用下的极限承载力,通过对不同荷载组合的数值计算构造了复合加载条件下沉箱基础破坏包络面。     

波浪作用下孔隙海床-管线动力相互作用分析

波浪作用下海床中的孔隙水压力与有效应力是影响海底管线稳定性的主要因素。然而,在目前的海床响应分析中一般将管线假定为刚性,并不能合理地考虑海床与管线之间的相互作用效应,同时也没有考虑土体和管线加速度对海床动力响应的惯性影响,从而无法确定由此所引起的管线内应力。为此考虑管线的柔性,分别采用饱和孔隙介质的Biot动力固结理论和弹性动力学理论列出了海床与管线的控制方程,进而采用摩擦接触理论考虑海床与管线之间的相互作用效应,基于有限元方法建立了海床-管线相互作用的计算模型及其数值算法。通过变动参数对比计算讨论了管线几何尺寸、海床土性参数对波浪所引起的管线周围海床孔隙水压力和管线内应力的影响。     

Application of Strontium Isotopic Stratigraphy to DatingMarine Sedimentary Units: A Case Study from the PermianStratotype Section in Southern China

The calibration of sedimentary rock absolute dates is one of the difficulties in sedimentological and stratigraphic research. Since strontium(Sr) resides in seawater much longer(≈106 a) than the seawater intermixing time(≈103 a), the Sr isotopic composition of global seawater is uniform at any time and results in a stable system throughout geological history,based on which a global Sr isotope composition dating database has been established for age-calibration of marine strata.The Permian stratigraphic sections in the northern part of the Upper Yangtze block, southern China, record continuous marine sediments with clear stratigraphic boundaries and is suitable for stratigraphic dating of Sr isotopes. Based on sampling and Sr isotopic compositions of Permian carbonate strata in the northern part of the Upper Yangtze, a Permian Sr isotope evolution curve was established. According to the basic principles of Sr isotope stratigraphy, the global Strontium isotope age database can be used to calibrate the Permian stratigraphic dates in the northern Upper Yangtze. The results show that the Sr isotope evolution curves for the marine carbonate rocks in the Permian stratigraphic section of the Upper Yangtze present a decreasing trend from the mid-Qixia stage(P2) to the mid-Wujiaping stage(P3), and then rise from the middle Wujiaping stage to the end of Changxing stage(P3). When the Permian Sr-isotope evolution curve is compared with the global Sr isotope evolution curve in the northern Upper Yangtze, the two are consistent in their long-term evolutionary trend, indicating that Permian global geological events are important controlling factors for the composition and evolution of Sr isotopes. The 87 Sr/86 Sr value decreased gradually in the background of large-scale regressions at the turn of middle to late Permian period, revealing that the Emeishan basalt eruption occurred near the Maokou/Wujiaping boundary(GLB). Srisotope stratigraphy dating was performed on the boundaries of the Qixia Formation/Maokou Formation, Maokou Formation/Wujiaping Formation(GLB), Wujiaping Formation/Changxing Formation(WCB) and the Permian/Triassic(PTB) using the Global Strontium Isotope Age Database. The results are 270.4 Ma, 261.2 Ma, 254.5 Ma and 249.7 Ma,respectively. Based on this, the eruption age of the Emeishan basalts is defined at about 261.2 Ma., which is more coincident with that acquired from other previous dating methods on the eruption age of the Emeishan basalts, and therefore proves that the application of Sr isotopic stratigraphy to dating marine sedimentary units is an effective method.     

养殖黄颡鱼(Pelteobagrus fulvidraco) 鮰爱德华氏菌(Edwardsiella ictaluri)的分离鉴定与生物学特性研究

从四川眉山与新津两地患红头病的养殖黄颡鱼体内分离到2株优势菌(CHNYC001与CHNYC002),以腹腔注射与浸泡的方式进行人工感染试验,证实其为养殖黄颡鱼红头病的病原菌。根据分离菌株的形态、生理生化特性,结合16SrDNA序列测定(GenBank登录号分别为FJ766524、FJ766525)与系统发育分析,将其鉴定为鮰爱德华氏菌(Edwardsie lla ictaluri)。分离菌株最适生长温度为20—30℃,最适生长pH为7.0,在含盐量2%以上的培养基上不生长,对氟苯尼考、强力霉素、洛美沙星等敏感,对乙酰螺旋霉素、磺胺甲基异噁唑和麦迪霉素等不敏感。临床病理特征分析发现,养殖黄颡鱼感染爱德华氏菌临床上分为急性与慢性两种类型,急性型主要表现为败血症的病变特征,慢性型表现为头顶出血,发红与溃疡的病变特征。     

广西涠洲岛珊瑚礁物种生物多样性研究

涠洲岛石珊瑚在属级组成中以角蜂巢珊瑚属(Favites)、滨珊瑚属(Porites)、蔷薇珊瑚属(Montipora)为优势类群,在科级的组成上,以蜂巢珊瑚科(Faviidae)、滨珊瑚科(Poritidae)、鹿角珊瑚科(Acroporidae)为优势类群。西南部的主剖面W2、东北部的W5的石珊瑚属种最多,说明了两个剖面海域石珊瑚生物多样性程度相对较高,这与涠洲岛历年来的珊瑚礁分布有关;涠洲岛6条主剖面的多样性指数均呈现一致的规律:W5W2W6W1W3W4,W2、W5多样性指数相对较高;珊瑚属种数量、优势属种数量、优势属种相对优势程度(最大的重要值百分比)、优势属重要值百分比之和分别与多样性指数的相关分析结果表明,珊瑚属种数量、优势属种数量对多样性指数有促进作用,珊瑚属种数量对多样性指数的贡献率更大;Simpson多样性指数(D)、Pielou均匀度指数(E)差异不显著,Shannon-Wienner多样性指数(H′)差异显著;整个岛区珊瑚属种分布比较均匀。     

欧洲鳗鲡肝肾病病原菌的分离与鉴定

患肝肾病的养殖欧洲鳗鲡(Anguilla anguilla)出现肝、肾肿大,且肝脏具白色溃疡灶的症状。从肝脏中分离、纯化到优势细菌,定名AL60306NA1。人工感染试验证实:向欧洲鳗鲡腹腔内注射1.0×108cfu/mL菌株悬液,实验鱼死亡率达100.0%;注射1.0×107cfu/mL,死亡率为60.0%;形态学观察、API20E细菌鉴定试剂盒鉴定实验证明菌株AL60306NA1有动力、拥有革兰氏反应阴性、氧化酶阴性、H2O2酶阳性、兼性厌氧、发酵葡萄糖产酸产气、不发酵蔗糖和蜜二糖及L一阿拉伯糖、赖氨酸脱羧酶阳性、柠檬酸盐弱阳性、产生硫化氢和吲哚、MR阳性和典型的β-溶血等特征;16S rRNA特异性基因分析鉴定实验说明克隆的AL60306NA1 16S rRNA基因部份序列为1507bp、与迟缓爱德华氏菌(AB050829.1)的同源性达99.7%。综合上述结果,确定该菌株为养殖欧洲鳗鲡肝肾病的病原菌,并鉴定为迟缓爱德华氏菌(Edwardsiella tarda)野生型。用菌株AL60306NA1感染小白鼠实验测定该菌对小白鼠的LD50为7.1×105cfu/mL,同时自该菌株PCR扩增到长度约1000bp的溶血素基因特异性片段,结果表明菌株AL60306NA1有一定的致病力。     

南海海域红斑后海螯虾(Metanephrops thomsoni)的分子识别与群体遗传分化

利用16S rRNA基因序列分子识别红斑后海螯虾,然后对海南附近海域(18°30′-19°00′N,111 °30′-112°30′ E)、北部湾口海域(15°41′ N,110°40′ E)和南沙群岛海域(5°20′-5°29′N,110°09′-111°26′E)三个地理群体进行ITS1的扩增测序.16S rRNA基因序列的结果表明所取样本准确;三个地理群体分别得到616-623bp、619bp和614bp的ITS1全长序列,A、G、T、C含量平均分别为22.3％、29.4％、17.8％和30.6％;其中562个保守位点,39个多态位点.ML和NJ聚类分析显示三个地理群体共聚为三个大支,其中海南群体有群体分化.表明红斑后海螯虾三个地理群体分化极其显著,且海南群体具有相对高的遗传多样性.