Implications of spatial heterogeneity for management of marine protected areas (MPAs): examples from assemblages of rocky coasts in the northwest Mediterranean

Marine protected areas (MPAs) are increasingly used as a management tool to preserve species and habitats. Testing hypotheses about the effectiveness of MPAs is important for their implementation and to identify informative criteria to support management decisions. This study tested the general proposition that MPAs affected assemblages of algae and invertebrates between 0.0 and 0.5 m above the mean low water level of rocky coasts on two islands in the Tuscan Archipelago (northwest Mediterranean). Protection was concentrated mainly on the west coasts of the islands, raising the possibility that neither the full range of assemblages nor the relevant scales of variation were properly represented within MPAs. This motivated the comparison of assemblages on opposite sides of islands (habitats). The effects of MPAs and habitat were assessed with a multifactorial sampling design; hypotheses were tested about differences in structure of assemblages, in mean abundance of common taxa and in univariate and multivariate measures of spatial variation. The design consisted of three replicate shores for each condition of protected and reference areas on the west side of each island and three unprotected shores on the eastern side. Assemblages were sampled independently four times on each island between June 1999 and January 2001. At each time of sampling two sites were selected randomly at each of two tidal heights to represent midshore and lowshore assemblages on each shore. Estimates of abundance were obtained using non-destructive sampling methods from five replicate 20x20 cm quadrats at each site. Results indicated differences among habitats in structure of assemblages, in mean abundance of common taxa and in univariate and multivariate measures of spatial variation at the scale of shores. Most of these patterns were inconsistent with the predicted effect of management through MPAs. The data suggest that designation of MPAs in the Tuscan Archipelago should proceed through management of multiple shores and types of habitat selected to guarantee protection to a representative sample of assemblages and to the processes responsible for maintenance of spatial patchiness at different scales. This study also shows that considerations of spatial heterogeneity are important to underpin management decisions about the number, size and location of MPAs.     

荧光法遥感海面叶绿素浓度的波段选择研究

本文从荧光波段范围内的大气效应出发,提出了可能应用于荧光法遥感海面叶绿素浓度的波段。并且用同一高度的航空遥感资料建立了荧光线高度值随波段中心位置和带宽变化的定量线性模式。其次,通过对不同度的航空遥感资料分析,提出了最佳波段选择的判别式。最后得到了两组最佳波段,基本上能满足遥感海面叶绿素α(文中简称叶绿素浓度)的使用要求。     

Alteration of the biochemical properties of female gonads and vitellins in the clam Mya arenaria at contaminated sites in the Saguenay Fjord

Vitellins (Vn) are the major egg yolk proteins that constitute an important energy reserve for mollusc embryos. The purpose of the present study was to examine whether the relative levels of sugars, lipids, phosphates, and labile IIb metals and calcium normally associated with Vn would differ in clam populations living at contaminated sites. Softshell clams (Mya arenaria) were collected at three sites in the area of the Saguenay Fjord: a marina, a municipal sewer outfall zone, and a reference site. The condition factor (weight:length ratio), metallothionein-like proteins, cytochrome P450 1A1 activity and DNA damage were all determined in the clam's digestive gland. Levels of total sugars, lipids, alkali-labile phosphates, proteins, and labile zinc and calcium were determined in female gonad homogenates and in purified Vn. The results show that clam gonads at the contaminated site by a marina displayed a lower index of gonad activity than the reference site. In addition, the condition factor was significantly lower at the marina site as compared to the reference site, with a concomitant increase in DNA damage and metallothionein (MT) induction. In fact, the condition factor was significantly correlated with DNA damage (R = -0.413, P = 0.045) and MT levels (R = -0.622, P = 0.03). Homogenates of female gonads were found to contain higher levels of labile IIb metals and calcium, with lower lipid content at the marina site compared to the reference site, and much higher levels of alkali-labile phosphates (ALP) and calcium at the municipal outfall site. Vn from the marina site were significantly higher in labile IIb metals but lipid content appeared to be somewhat lower than at the reference site. Vn from the municipal site were found to be highly phosphorylated, with higher levels of lipids and calcium. These results suggest that the chemical composition of the gonads and Vn are altered in contaminated sites.     

Sublethal toxicity of orchard stormwater runoff in Sacramento splittail (Pogonichthys macrolepidotus) larvae

The sublethal effects of stormwater runoff from sections of a plum orchard treated with esfenvalerate or diazinon were evaluated in 7-day-old Sacramento splittail (Pogonichthys macrolepidotus) larvae. Fish were exposed to eight runoff samples using the USEPA standard static renewal method for 96 h acute toxicity testing, then transferred to clean water for three-month to assess the survival, growth, histopathological abnormalities, and heat stress proteins (hsp). No significant mortality was observed at 96 h in exposed fish. At one week, histopathological abnormalities included severe glycogen depletion, cytoplasmic protein droplets, vacuolar degeneration, and cell necroses in liver of all exposure groups. Pyknotic nerve cells were seen in brain of one exposure group. Significantly higher cumulative mortality, lower condition factor, and elevated hsp60 and hsp70 levels (p < 0.05) were occurred in several exposure groups. No histopathological abnormalities were observed after three months in any exposure group. This study confirms that standard acute toxicity tests have underestimated the toxicity of stormwater runoff, and although splittail larvae survived the 96 h exposure, they exhibited reduced survival and growth and showed signs of cellular stress even after a three month recovery period.     

Meiofauna as descriptor of tourism-induced changes at sandy beaches

Tourism has long been considered as a 'clean industry' with almost no negative effects on the environment. This study demonstrated, in two different coastal systems (Mediterranean and Baltic), that tourism related activities are particularly affecting the sandy beach meio- and nematofauna in the upper beach zone, the specific ecotone in which many meiofauna species from both the marine and the terrestrial environment congregate. Tourist upper beaches are characterized by a lower % total organic matter (%TOM), lower densities, lower diversities (absence of Insecta, Harpacticoida, Oligochaeta, terrestrial nematodes and marine Ironidae nematodes) and higher community stress compared to nearby non-tourist locations. The %TOM was found to be the single most important factor for the observed differences in meiofauna assemblage structure at tourist versus non-tourist beaches in both the Mediterranean and the Baltic region. The free-living nematode assemblages from tourist upper zones depart significantly from expectations based on random selections from the regional nematode species pool. Furthermore upper zone assemblages are characterised by a low species diversity consisting of taxonomically closely related nematode species with r-strategist features. Generally, faunal differences between tourist and non-tourist beaches are decreasing towards the lower beach zones.     

Paleomagnetism and paleoclimate change in the South China Sea since the late Pleistocene

Signal processing techniques of cross-correlation, power spectral analysis and cross-power spectral analysis are used to determine the relationships between paleomagnetic properties and the oxygen isotope record of the South China Sea during the late Pleistocene. On the basis of correlation tests between the δ18O time series of cores V3 and A15 with the paleomagnetic properties of core SCS01, five events of susceptibility and intensity of natural remanent magnetization are identified as glacial events corresponding to δ18O positive events. The magnetic properties of the sediments which are independent of the geomagnetic field are sensitive climatic indicators which have frequencies corresponding to the Milankovitch orbital parameters.     

Rhodamine exclusion activity in primary cultured turbot (Scophthalmus maximus) hepatocytes

Cellular detoxification by direct processes has been investigated in fish by studying the ability of hepatocytes prepared from juvenile aquarium-reared turbot (Scophthalmus maximus) to actively exclude the fluorescent dye rhodamine B (RB). Cell viability was studied by measurements of non-specific esterase activity using fluorescein diacetate. This revealed that turbot hepatocytes can be cultured for a few days with a viability decreasing to 38% after 24 h. The 24-h cultured cells have been used to study the rhodamine B exclusion activity using confocal laser microscopy. Hepatocytes accumulated the dye in a competitive manner with verapamil, thus suggesting that they express a transport system similar to the P-glycoprotein-mediated multixenobiotic resistance process. Incubation of cells with 1 microM RB and 20 microM verapamil led to a 26% increase of cellular fluorescence as compared to the accumulation in absence of competitor. Rhodamine B accumulated in the whole cytoplasm, with more concentrated areas that might correspond to the lysosomal compartment and the cell membrane.     

E24 profile slope stability analysis in Haizhou Opencast Coal Mine of Fuxin

The E24 profile slope analyzed belongs to a series of excavated slopes of the Haizhou Opencast Coal Mine. It seems to be divided into Downslope Part and Upslope Part. Its profile comprises two noticeable coal seams, called the 8# and 9# weak layers, considered as the potential failure surfaces. In consideration of the actual configuration as in the perspective of any modification, assessing the stability of this slope with various profile forms under given conditions, and assessing the risk of instability and quantifying the influence of earthworks or other modifications to the stability of this slope, have constituted the primordial objectives carried out. From assumed potential failure surfaces, any specific profiles and specified slip surfaces are defined. A factor of safety (FoS) is computed for each specified slip surface; the smallest FoS found corresponds to the least favorable slip surface. The safety factor values obtained are compared to the suggested safety factor. Limit equilibrium methods of vertical slices implemented in Slope/W, computer program for slope stability analyses, have been adopted to perform the E24 slope stability analysis. The safety factor values computed with 9# weak layer are lower than for 8#; the factors of safety obtained with Sarma's method are the smallest; more, without groundwater (long term) overall values are greater than those determined under groundwater condition (short term). The lowest safety factor value is found for a profile depending on an adopted earthwork sequence. The E24 profile slope stability analysis shows the instability risk for the deepest weak layer, and also shows the short and long term stability of this slope for the envisaged earth movements. However it demonstrates the existence of instability risk for any earthwork firstly affecting the downslope part.