Implementation of microbial processes in the performance assessment of spent nuclear fuel repositories

Present strategies for the long-term disposal of high-level nuclear wastes are based on the construction of repositories hundreds of meters below the earth surface. Although the surrounding host-rocks are relatively isolated from the light at the earth surface they are by no means lifeless. Microorganisms rule the deep part of the biosphere and it is well established that their activity can alter chemical and physical properties of these environments. Microbial processes can directly and indirectly affect radionuclide migration in multiple ways. Within 6th FP IP FUNMIG the interplay between microbial biofilms and radionuclides and the effect of microbially induced redox transformations of Fe on radionuclide mobility have been investigated. For the first time, formation of U(V) as a consequence of microbial U(VI) reduction in a multi-species biofilm was detected in vivo by combining laser fluorescence spectroscopy and confocal laser scanning microscopy. Furthermore, it was demonstrated that addition of U(VI) can lead to increased respiratory activity in a biofilm. Increased respiration in a biofilm can create microenvironments with lower redox potential, and hence induce reduction of radionuclides. Transient mobilization of U was observed in experiments with Fe oxides containing adsorbed U(VI) in which the activity of SO₄-reducing organisms was mimicked by sulfide addition. Faster reaction of sulfide with Fe oxides compared to U(VI) reduction, and decreasing U(VI) adsorption due to the transformation of Fe oxides into FeS can explain the observed intermittent U mobilization. The presented research on microbe-radionuclide interactions performed within FUNMIG addresses only a few aspects of the potential role of microorganisms in the performance assessment of nuclear waste repositories. For this reason, additionally, this article provides a cursory overview of microbial processes which were not studied within the FUNMIG project but are relevant in the context of performance assessment. The following aspects are presented: (a) the occurrence and metabolic activity of microorganisms of several proposed types of host-rocks, (b) the potential importance of microorganisms in the near-field of nuclear waste repositories, (c) indirect effects of microbial processes on radionuclide mobility in the repository far-field, (d) binding of radionuclides to microbial biomass, (e) microbial redox transformations of radionuclides, and (f) the implementation of microbial processes in reactive transport models for radionuclide migration.     

Cadastralizing or coordinating the clam commons: Can competing community and government visions of wild and farmed fisheries be reconciled?

This paper considers socio-political, ecological, and economic dimensions of local efforts to negotiate local control over state-sponsored development of clam aquaculture in one region of British Columbia, Canada. Aquaculture is conceptualized as a type of cadastralization, following James Scott's characterization of state efforts to make the productivity of landscapes more measurable for purposes of rent generation and taxation. The discussion invites a rethinking of community resistance to development as less about the notion of development itself than about the terms under which it occurs, since the community engages in its own form of cadastralization as a negotiating strategy. The concept of cadastralization enriches common property theory approaches to ownership and management by highlighting the key role of technological innovations in allowing global market forces to more easily penetrate local property relationships. At the same time it enables new forms of resistance and assertion of local visions of development.     

Reviewing the Guayana project: Lifetime migration to and from Bolivar state,Venezuela, 1950–2001

By the mid‐twentieth century the population and economic resources in many developing economies had become concentrated in the capital city. Planned industrial cities became an important mechanism to encourage more balanced urban systems as well as development in peripheralized regions. Venezuela boasts one of the most impressive planned industrial cities, Ciudad Guayana, constructed in 1959 in the resource rich but inaccessible Bolivar state in the southeast. This paper summarizes the lifetime migration to and from Bolivar state for the years 1950, 1971 and 2001. Whereas the majority of lifetime migrants originated from the neighbouring northeast region in 1950 and 1971, by 2001 more migrants arrived from the capital region. For lifetime outmigrants, the destination states became more diverse and less focused on the capital region. Gender ratios of lifetime migrants to and from Bolivar became more equitable as women became more prominent in migration flows. The level of urban primacy in Venezuela declined substantially after 1971 as the country became more internally integrated, although this more equitable distribution of the country's population may not have been solely a result of the creation of the growth pole, but a result of wider economic development.     

Control sample design using a geodemographic discriminator: An application of Super Profiles

 The development and application of an innovative sampling framework for use in a British study of the early detection of gastric cancer are described. The Super Profiles geodemographic discriminator is used in the identification of geographically distinct control and contrast areas from which samples of cancer registry case records may be drawn for comparison with the records of patients participating in the gastric cancer intervention project. Preliminary results of the application of the framework are presented and confirm its effectiveness in satisfactorily reflecting known patterns of variation in cancer occurrence by age, gender and social class. The method works well for cancers with a known and clear social gradient, such as lung and breast cancer, moderately well for gastric cancer and somewhat less well for oesophageal cancer, where the social class gradient is less clear. Received: 1 January 2000 / Accepted: 18 September 2000     

The transport of xenoliths in magmas

Field measurements of the rheology of Hawaiian and Etnean lavas have shown that, on eruption, they behave as Bingham liquids with yield strengths in the range 70–450 N/m². Ultramafic nodules entrained in a Bingham liquid cannot settle unless the stress they impose on the liquid exceeds approximately 5–7 times its yield strength. Consequently magmas with yield strengths of 10–1000 N/m² can transport xenoliths up to 30 cm diameter without settling occurring. The size of nodules commonly observed rarely exceeds 30 cm. A review of experimental data shows that, when conditions are appropriate for settling, the terminal velocities of nodules in magmas are substantially slower if a Bingham rather than Newtonian Model is assumed.The view that large nodules imply fast rates of magma ascent is rejected. A case is presented for slow rates of ascent being more suitable for nodule transport as there is more opportunity for cooling, crystallisation and hence development of a yield strength. The relative abundance of nodules in the alkaline suite may be a consequence of their slow rates of ascent, whereas their absence in tholeiitic melts may be a consequence of rapid ascent rates. This interpretation is compatible with deductions on their relative rates of ascent based on other geological evidence.     

The evolution of lava flow-fields: observations of the 1981 and 1983 eruptions of Mount Etna,Sicily

The eruptions of Mount Etna in 1981 on the north flank and 1983 on the south flank of the volcano were of strikingly different character. The former was a short duration, high effusion rate eruption producing for the most part a simple flow-field; the latter was of relatively long duration and low effusion rate, producing a compound flow-field of overlapping flows.Despite the differences between the eruptive behaviour of these two events and the way in which the flow-field developed, both the flow-fields achieved about the same maximum length. This is considered fortuitous. The evidence suggests that the main 1981 flow stopped because the lava supply ceased and was thus volume controlled. The 1983 flow-field had a more complex history of branching, but in this case it appears that, for the longest individual flow, cooling played an important role in controlling the maximum extent of the flows.     

Oblique photogrammetry with visible and thermal images of active lava flows

Digital images from hand-held cameras are increasingly being acquired for scientific purposes, particularly where non-contact measurement is required. However, they frequently consist of oblique views with significant camera-to-object depth variations and occlusions that complicate quantitative analyses. Here, we report the use of oblique photogrammetric techniques to determine ground-based thermal camera orientations (position and pointing direction), and to generate scene information for lava flows at Mount Etna, Sicily. Multiple images from a consumer grade digital SLR camera are used to construct a topographic model and reference associated ground-based thermal imagery. We present data collected during the 2004–2005 eruption and use the derived surface model to apply viewing distance corrections (to account for atmospheric attenuation) to the thermal images on a pixel-by-pixel basis. For viewing distances of ~100 to 400 m, the corrections result in systematic changes in emissive power of up to ±3% with respect to values calculated assuming a uniform average viewing distance across an image.     

How Does the Species Used for Calibration Affect Chlorophyll <Emphasis Type="Italic">a</Emphasis> Measurements by In Situ Fluorometry?

We determined how the species used for calibration affects the accuracy of in situ chlorophyll a (chl a) measurements by fluorometry using single-species cultures and natural phytoplankton populations from Winyah Bay, South Carolina, USA. When a diatom was used for calibration, chl a in a dinoflagellate culture was overestimated by 66 ± 7%, whereas concentrations of a cryptophyte, chlorophyte, and cyanobacterium were underestimated by 16 ± 20%, 40 ± 7%, and 71 ± 33%, respectively. In natural populations, the combination of species-specific and environmentally induced variation in the ratio of fluorescence to chl a (F Chl⁻¹) led to an overestimate by the in situ fluorometer of 40–169% for an April experiment and an underestimate of 4–50% in July. Even when field samples were dominated by diatoms, environmental effects resulted in highly variable predictions of chl a. Thus, while a carefully selected calibration species can improve estimates of in vivo chl a in the laboratory, calibration of in situ fluorometers should be done with natural communities collected from the site of interest.     

The SOLAS air-sea gas exchange experiment (SAGE) 2004

The SOLAS air-sea gas exchange experiment (SAGE) was a multiple-objective study investigating gas-transfer processes and the influence of iron fertilisation on biologically driven gas exchange in high-nitrate low-silicic acid low-chlorophyll (HNLSiLC) Sub-Antarctic waters characteristic of the expansive subpolar zone of the southern oceans. This paper provides a general introduction and summary of the main experimental findings. The release site was selected from a pre-voyage desktop study of environmental parameters to be in the south-west Bounty Trough (46.5°S 172.5°E) to the south-east of New Zealand and the experiment was conducted between mid-March and mid-April 2004. In common with other mesoscale iron addition experiments (FeAX’s), SAGE was designed as a Lagrangian study, quantifying key biological and physical drivers influencing the air-sea gas exchange processes of CO₂, DMS and other biogenic gases associated with an iron-induced phytoplankton bloom. A dual tracer SF₆/³He release enabled quantification of both the lateral evolution of a labelled volume (patch) of ocean and the air-sea tracer exchange at tenths of kilometer scale, in conjunction with the iron fertilisation. Estimates from the dual-tracer experiment found a quadratic dependency of the gas exchange coefficient on windspeed that is widely applicable and describe air-sea gas exchange in strong wind regimes. Within the patch, local and micrometeorological gas exchange process studies (100 m scale) and physical variables such as near-surface turbulence, temperature microstructure at the interface, wave properties and windspeed were quantified to further assist the development of gas exchange models for high-wind environments.There was a significant increase in the photosynthetic competence (F_v/F_m) of resident phytoplankton within the first day following iron addition, but in contrast to other FeAX’s, rates of net primary production and column-integrated chlorophyll a concentrations had only doubled relative to the unfertilised surrounding waters by the end of the experiment. After 15 days and four iron additions totalling 1.1 ton Fe²⁺, this was a very modest response compared to other mesoscale iron enrichment experiments. An investigation of the factors limiting bloom development considered co-limitation by light and other nutrients, the phytoplankton seed-stock and grazing regulation. Whilst incident light levels and the initial Si:N ratio were the lowest recorded in all FeAXs to date, there was only a small seed-stock of diatoms (less than 1% of biomass) and the main response to iron addition was by the picophytoplankton. A high rate of dilution of the fertilised patch relative to phytoplankton growth rate, the greater than expected depth of the surface mixed layer and microzooplankton grazing were all considered as factors that prevented significant biomass accumulation. In line with the limited response, the enhanced biological draw-down of pCO₂ was small and masked by a general increase in pCO₂ due to mixing with higher pCO₂ waters. The DMS precursor DMSP was kept in check through grazing activity and in contrast to most FeAX’s dissolved dimethylsulfide (DMS) concentration declined through the experiment. SAGE is an important low-end member in the range of responses to iron addition in FeAX’s. In the context of iron fertilisation as a geoengineering tool for atmospheric CO₂ removal, SAGE has clearly demonstrated that a significant proportion of the low iron ocean may not produce a phytoplankton bloom in response to iron addition.     

Multi-method assessment of connectivity between surface water and shallow groundwater: the case of Limarí River basin,north-central Chile

A study that tests the applicability and consistency of independent but complementary approaches in the assessment of interactions between surface water and shallow groundwater within a water-stressed basin is described. The mostly agricultural Limarí basin in arid north-central Chile was chosen as a suitable case study. The analyses involved: (1) a connectivity index method, (2) hydrochemistry, and (3) water isotopic geochemistry. Chemical and isotopic data were obtained from two sampling campaigns conducted in April (fall) and December (summer) of 2011 in 22 sampling locations, which included surface water and groundwater. The results obtained by each of the methodologies were mutually consistent and indicate high connectivity conditions. Additionally, the relative contribution by different sources was assessed through end-member mixing analysis, and for reaches of the river that showed gaining conditions, the contribution of groundwater inflow to stream discharge was estimated. It is suggested that this multi-method approach is useful for the characterization of surface-water–groundwater interactions, since it at least represents a suitable starting point for obtaining basic information on these relationships. Thus, it may become the base for further studies in arid and semi-arid basins facing water management challenges.