Effects of polychlorinated biphenyls on liver function and sexual characteristics in Japanese medaka (Oryzias latipes)

The effects of polychlorinated biphenyls (PCBs) on liver function and their differences between sexes were analyzed in Japanese medaka (Oryzias latipes) exposed to PCB126 or Kanechlor-400 (KC-400) using microarray. PCB exposure induced vitellogenin 1 expression in female medaka while suppressing choriogenin genes, which suggests that the effects of PCBs on estrogen-responsive genes do not occur directly through an estrogen receptor-mediated pathway. Reduction of androgen receptor alpha expression was also observed, and the gene expression pattern in PCB-exposed males changed to become more similar to that of females. Furthermore, changes in glycolysis-related genes indicate that PCB exposure might enhance glucose production via gluconeogenesis in the liver of medaka. Taken together, our results suggest that PCBs disrupt the endocrine system, especially androgen function, and may have the potential to cause demasculinizing effects. Additionally, induction of gluconeogenesis might be a response to maintain glucose levels consumed as a result of PCB exposures.     

Vertical and horizontal distribution of fluorescent dissolved organic matter in the Southern Ocean

The vertical and horizontal distribution of fluorescent dissolved organic matter (FDOM), determined by fluorescence intensity at 320 nm excitation and 420 nm emission, were clarified in nine stations on two transects at the Southern Ocean, including a subtropical, subantarctic, polar frontal and Antarctic zone. All vertical profiles of fluorescence intensity showed that levels were lowest in the surface waters, increased with increasing the depth in mid-depth waters ( 2000 m), and then stayed within a relatively narrow range from there to the bottom. Such vertical profiles of FDOM were similar to those of nutrients, but were adverse to dissolved oxygen. In water columns below the temperature-minimum subsurface water (dichothermal waters) in the Antarctic zone and below the winter mixed layer in the other zones, we determined the relationships of fluorescence intensity to concentrations of nutrients and apparent oxygen utilization (AOU) over the entire area of the present study, and found significant linear correlations between the levels of fluorescence intensity and nutrient concentrations (r =  0.70 and 0.71 for phosphate and nitrate + nitrite, respectively) and AOU (r = 0.91). From the strong correlation coefficient between fluorescence intensity and AOU, we concluded that FDOM in the Southern Ocean is formed in situ via the biological oxidation of organic matter. The regeneration of the nutrients/consumption of the oxygen/formation of FDOM was active in mid-depth waters. However, the correlations between fluorescence intensities and nutrients and AOU were different in the mid-depth water masses, Subantarctic Mode Water (SAMW), and Antarctic Intermediate Water (AAIW), indicating that the sources of organic matter responsible for FDOM formation were different. A considerable amount of FDOM in the SAMW is thought to be produced by the remineralization of DOM in addition to sinking particulate organic matter, while DOM is less responsible for FDOM formation in the AAIW.     

Distributional characteristics of DOC in the central Equatorial Pacific

Distributions of dissolved organic carbon (DOC) as determined by high temperature catalytic oxidation (HTCO) method, are reported for 5 stations located from 15°N to 5°S along the transect of 180° in the central Equatorial Pacific. Vertical profiles of DOC suggest that levels of DOC in the photic layer at a given station are dependent on the vertical stability of the water column and meridional currents in the central Equatorial Pacific. Concentrations of DOC determined by the HTCO method in the northern North to Equatorial Pacific and those of total organic carbon (TOC) determined by Wet Chemical Oxidation (WCO) method during the GEOSECS Pacific cruises indicated that DOC determined by both methods, taken together with the levels of DOC in the surface water, showed clear meridional differences in the areas from north of 40°N to the equatorial region in the Pacific: values were low at high latitudes, namely, in productive areas, but high at low latitudes, namely, in oligotrophic areas, even though the values of DOC determined by the two methods were different.     

Distribution and alteration of amino acids in bulk DOM along a transect from bay to oceanic waters

A systematic survey of the concentrations and composition of total hydrolyzable amino acids (THAA) in bulk dissolved organic matter (DOM) was conducted at 11 stations along the 137°E transect from bay to oceanic areas in the northwestern Pacific. Concentrations of THAA and their contributions to dissolved organic carbon were high in the bay and coastal areas, declined toward the oceanic area and decreased with depth in the water columns. From the distribution patterns of the relative abundances of amino acids along the transect, individual amino acids were divided into four groups. One group included tyrosine, valine, isoleucine, phenylalanine, leucine and tryptophan, and was considered to represent easily degradable THAA, while glycine and alanine belonged to a more biorefractory group of THAA. Principal components analysis (PCA) was conducted to quantitatively differentiate patterns of amino acid composition. Amino acid groups based on PCA agreed with the groups classified by distribution patterns, indicating that first principal component scores reflected the degree of degradation of THAA in DOM, and were defined as a degradation index (DI). Two amino acids, glycine and alanine, increased in relative abundance with increasing DI, while valine, isoleucine, phenylalanine and leucine decreased with decreasing DI. The agreement indicated that the degradation process was the key factor controlling the quantity and quality of THAA in bulk DOM.     

海底地震学

在海洋地区进行高质量的长期地震观测是全球地震观测的一个重要组成部分，DSDP／ODP是唯一能够钻穿软的沉积物、在坚硬的岩石里安置地震传感器这一目标的科学计划。介绍了ODP航次在井孔中设立地震台站、并获得一些有趣的结果的成功例子。ODP在日本外海布置了两个井中地震台站，与陆上台站一起来观测板块边界的活动性。此外，还介绍了西太平洋井中宽带台站、海上地震信号和噪音等问题。     

Seismological structure and implications of collision between the Ontong Java Plateau and Solomon Island Arc from ocean bottom seismometer–airgun data

A seismic refraction–reflection experiment using ocean bottom seismometers and a tuned airgun array was conducted around the Solomon Island Arc to investigate the fate of an oceanic plateau adjacent to a subduction zone. Here, the Ontong Java Plateau is converging from north with the Solomon Island Arc as part of the Pacific Plate. According to our two-dimensional P-wave velocity structure modeling, the thickness of the Ontong Java Plateau is about 33 km including a thick (15 km) high-velocity layer (7.2 km/s). The thick crust of the Ontong Java Plateau still persists below the Malaita Accreted Province. We interpreted that the shallow part of the Ontong Java Plateau is accreted in front of the Solomon Island Arc as the Malaita Accreted Province and the North Solomon Trench are not a subduction zone but a deformation front of accreted materials. The subduction of the India–Australia Plate from the south at the San Cristobal Trench is confirmed to a depth of about 20 km below sea level. Seismicity around our survey area shows shallow (about 50 km) hypocenters from the San Cristobal Trench and deep (about 200 km) hypocenters from the other side of the Solomon Island Arc. No earthquakes occurred around the North Solomon Trench. The deep seismicity and our velocity model suggest that the lower part of the Ontong Java Plateau is subducting. After the oceanic plateau closes in on the arc, the upper part of the oceanic plateau is accreted with the arc and the lower part is subducted below the arc. The estimation of crustal bulk composition from the velocity model indicates that the upper portion and the total of the Solomon Island Arc are SiO₂ 58% and 53%, respectively, which is almost same as that of the Izu–Bonin Arc. This means that the Solomon Island Arc can be a contributor to growing continental crust. The bulk composition of the Ontong Java Plateau is SiO₂ 49–50%, which is meaningfully lower than those of continents. The accreted province in front of the arc is growing with the convergence of the two plates, and this accretion of the upper part of the oceanic plateau may be another process of crustal growth, although the proportion of such contribution is not clear.     

Tectonic evolution of the triple junction off central Honshu for the past 1 million years

We discuss several models of the evolution of the trench-trench-trench triple junction off central Honshu during the past 1 m.y. on the basis of plate kinematics, morphology, gravity and seismic reflection profile data available for the area. The study area is characterized by large basins, 7–8 km deep on the inner lower trench slope on the Philippine Sea side and the deep (9 km) Izu-Bonin Trench to the east. Between the basins and the trench, there are 6–7 km-deep basement highs. The triple junction is unstable due to the movement of the Philippine Sea plate at a velocity of 3 cm/yr in WNW direction with respect to Eurasia (Northeast Japan), subparallel to the strike of the Sagami Trough. Generally we can expect the boundary area between the Philippine Sea and Pacific plates to be extended because the Pacific plate is unlikely to follow the retreating Philippine Sea plate due to the obstruction of the southeastern corner of Eurasia. The above peculiar morphology of the junction area could have resulted from this lack of stability. However, there are several possible ways to explain the above morphology.

Our gravity model across the trench-basement high-basin area shows that the basement highs are made of low-density materials (1.8–2 g/cm³). Thus we reject the mantle diapir model which proposes that the basement highs have been formed by diapiric injection of serpentinites between the retreating Philippine Sea plate and the Pacific plate.

The stretched basin model proposes that the basins have been formed by stretching of the Philippine Sea plate wedge. We estimated the extension to be about 10 km at the largest basin. We reconstructed the morphology at 1 Ma by moving the Philippine Sea plate 20 km farther to the east after closing the basins, and thus obtained 8 km depth of the 1 Ma trench, which is similar to that of the present Japan Trench to the north. Although this stretched basin model can explain the formation of the basins and the deep trench, other models are equally possible. For instance, the eduction model explains the origin of the basin by the eduction of the Philippine Sea basement from beneath the basement high, while the accretion model explains the basement highs by the accretion of the Izu-Bonin trench wedge sediments. In both of these models we can reconstruct the 1 Ma trench depth as about 8 km, similar to that of the stretched basin model.

The deformation of the basement of the basins constitutes the best criterion to differentiate between these models. The multi-channel seismic reflection profiles show that the basement of the largest basin is cut by normal faults, in particular at its eastern edge. This suggests that the stretched basin model is most likely. However, the upper part of the sediments shows that the basement high to the east has been recently uplifted. This uplift is probably due to the recent (0.5 Ma) start of accretion of the trench wedge sediments beneath this basement high.     

A simulation of continental basin margin sedimentation in response to crustal movements, eustatic sea level change, and sediment accumulation rates

As eustasy, subsidence, and sediment accumulation vary, a 2D computer-based graphical simulation generates on-lapping and off-lapping geometries of both marine and near coastal alluvial deposits, reproducing timelines within sediment-bodies at basin margins. In the simulation, deposition is expressed by creation of new surfaces above previous ones. Thicknesses of layers are reduced by both erosion and compaction while their surfaces move vertically in response to tectonic change and loading. Simulation is divided into a series of equal time steps in which sediment is deposited as an array of en-echelon columns that mark the top of the previous depositional surface. The volume of sediment deposited in each time step is expressed as a 2D cross section and is derived from two right-angle triangles (sand and shale), whose areas are a 2D expression of the quantity of sediment deposited at that time step and whose length matches the width of the offshore sediment wedge seaward of the shoreline. Each column in the array is filled by both marine sediments up to sea level, and alluvial sediments to a surface determined by an alluvial angle that is projected landward from the shore to its intersection with the previous surface. Each time the area representing the sediment column is subtracted from the triangles, the triangle heights are reduced correspondingly. This process is repeated until the triangle heights match the position of sea level above the sediment surface, at which time the remaining area of the sediment triangle is deposited seaward as a single wedge of offshore sediments. This simulation is designed to aid interpretation of stratigraphic sequences. It can be used as a complement to seismic stratigraphy or can be used alone as an inexpensive test of stratigraphic models.