Application of the Genetic Algorithm to the Problem of Reconstruction of Missing Data

We study the problem of reconstruction (interpolation and extrapolation) of the vertical profiles of hydrochemical and hydrobiological elements according to incomplete sets of data with simultaneous filtration of short-period components based on expansions in empirical orthogonal functions. The genetic algorithm is used to compute the coefficients of expansion of profiles with missing data. We present the results of processing the data arrays on oxygen, chlorophyll A, and biogenic elements collected in the Black Sea in 1982–1993. The mean error of reconstruction of the profiles enables us to conclude that the proposed method has considerable advantages over the conventional approaches.     

Release into the environment of metals by two vascular salt marsh plants

Metals in contaminated salt marshes are mainly locked in the anaerobic layer of sediments, where they are tightly bound as sulfides and organic complexes. Vascular plants survive in saturated soils in part by pumping O2 into their root zones, changing their microenvironment to an oxic one. This, along with chelating exudates, mobilizes metals, allowing uptake by the roots. We compared the common reed Phragmites australis and cordgrass Spartina alterniflora in lab and field studies for ways in which they handle trace metals. Both plants store most of their metal burden in their roots, but some is transported to aboveground tissues. Spartina leaves contain approximately 2-3 x more Cr, Pb, and Hg than Phragmites leaves, but equivalent Cu and Zn. Furthermore, Spartina leaves have salt glands, so leaf excretion of all metals is twice that of Phragmites. In-depth studies with Hg indicate that Hg excretion correlates with Na release but not with transpiration, which is 2.2 x higher in Phragmites; and that more Hg accumulates in early-appearing leaves than in upper (i.e. later) leaves in both species. Spartina thus makes more metals available to salt marsh ecosystems than Phragmites by direct excretion and via dead leaves which will enter the food web as detritus.     

The expression of CYP1A,vitellogenin and zona radiata proteins in Atlantic salmon (Salmo salar) after oral dosing with two commercial PBDE flame retardant mixtures: absence of short-term responses

The short-term effects of the commercial PBDE flame retardant mixtures Penta-BDE and cta-BDE on the expression of cytochrome P450 1A (CYP1A), vitellogenin (Vtg) and zona radiata proteins (Zrp) were investigated in juvenile salmon (Salmo salar). For this purpose, groups of fish were dosed twice (oral intake at days I and 4) with 10 and 50 mg/kg body weight of both commercial mixtures. The fishes were sacrificed at day 7 (n = 5 for each group) and 14 (n = 6 for each group), and blood, liver, fillet, and brain were collected. Blanks and positive controls were also part of the experiment. The expressions of Vtg, Zrp, and CYPIA were measured with several techniques (EROD, ELISA, Western, Northern and Slot Blot). The values in the groups of fish treated with Penta-BDE or Octa-BDE did not significantly differ from the reference group for any of the parameters tested. In contrast, the positive control groups treated with estradiol-17beta for Vtg and Zrp expression, and beta-naphthoflavone for CYP1A expression did show a significant response, indicating the potential sensitivity of the fishes for the parameters measured. Since the results of the chemical analyses showed concentrations of a number of PBDE congeners in liver, fillet, and brain that were about three orders of magnitude above those of fish from the North Sea, it is concluded that the short-term toxicity of both commercial PBDE mixtures for these endpoints was low.     

Evaluation of acetylcholinesterase activity in several zooplanktonic crustaceans

Zooplankton are an essential component of the marine and estuarine food chains. The ecotoxicological risk to zooplanktonic communities, estimated through the use of the biomarkers, can be used as an early warning signal of a potential alteration of the ecosystem health. The aim of this project was to evaluate the potential use of several biomarkers (esterases, mixed function oxidases, porphyrins) in zooplanktonic organisms. The acetylcholinesterase activity (AChE) was determined in homogenates of whole organisms of 10 different zooplanktonic crustaceans. Mean activity of AChE was 10.05 micromol/min/g for Acartia margalefi; 3.30 for Acartia latisetosa; 79.70 for Siriella clausi; 49.97 for Diamysis bahirensis; 7.48 for Siriella armata; 14.20 for Mysidopsis gibbosa; 4.49 for Euphausia crystallorophias; 1.66 for Euphausia superba; 2.74 for Streetsia challengeri; 13.26 for Meganycthiphanes norvegica. The species moreover showed a linear increase in enzyme activity in relation to the increase in sample concentration. The key result of this study concerns the different AChE activity basal values of the crustaceans' different species.     

Control over natural resources in ecological economical systems

We consider the problem of stable development of natural economical complexes from the viewpoint of basic balances in an ecological economical system. We construct a basic dynamical model of the complex capable of predicting various scenarios of the industrial development, consumption of natural resources, and pollution of the environment and propose a mechanism of control over the process of development by introducing rent payments for the use of natural resources and ecological fines for the pollution of the environment. The efficiency of the applied model is demonstrated by analysing the process of control over the concentration of biological resources in marine media as an example. We describe the results of numerical experiments performed to show the possibility of creation of the information technology of control over marine bioresources. Translated by Peter V. Malyshev and Dmitry V. Malyshev     

Burial and scour around short cylinder under progressive shoaling waves

The results of a laboratory experimental program aimed at better understanding the scour around and burial of heavy cylindrical objects under oscillating flow on a sandy bed are described. This study was motivated by its application to the dynamics of isolated cobbles/mines on a sandy floor under nonlinear progressive waves, such as that occur in shallow coastal waters beyond the wave-breaking region. In the experiments, nonlinear progressive waves were generated in a long wave tank of rectangular cross-section with a bottom slope. Model mines (short cylinders) were placed on the sandy bottom and the temporal evolution of the bed profile and the velocity field in the near field of the object were observed. Experiments were conducted at relatively high Reynolds numbers for a range of flow conditions, which can be characterized by the Keulegan–Carpenter number and Shields parameter. Depending on the values of these parameters, four different scour regimes around the cylinder including periodical burial of cylinder under migrating sand ripples were observed; they were classified as: (i) no scour/burial, (ii) initial scour, (iii) expanded scour, and (iv) periodic burial cases. A scour regime diagram was developed and the demarcation criteria between different regimes were deduced. Semi-empirical formulae that permit estimation of the scour depth with time, the equilibrium maximum scour depth and length, and conditions necessary for the burial of the cylinder as a function of main external parameters are also proposed.     

Modelling of flow around a near-bed pipeline with a spoiler

Flow around a pipeline with and without a spoiler near a smooth wall is simulated by solving the Navier–Stokes equations. Finite-difference formulation with a second-order upwind scheme in a curvilinear coordinate system is employed. The influences of the spoiler on hydrodynamic forces, pressure distribution, vortex shedding frequency, velocity profile under the pipe, as well as shear stress on the wall are investigated. The attachment of a spoiler significantly increases drag, root-mean-square (RMS) lift, flow through the gap between the pipe and the wall and shear stress on the seabed around the pipe. The spoiler also generates a non-zero mean downward force on the pipeline, which may enhance the self-burial of the pipeline.     

Upstream dams and downstream clams: growth rates of bivalve mollusks unveil impact of river management on estuarine ecosystems (Colorado River Delta, Mexico)

We studied how the extensive diversion of Colorado River water, induced by dams and agricultural activities of the last 70 years, affected the growth rates of two abundant bivalve mollusk species (Chione cortezi and Chione fluctifraga) in the northern Gulf of California. Shells alive on the delta today (‘Post-dam’ shells) grow 5.8–27.9% faster than shells alive prior to the construction of dams (‘Pre-dam’ shells). This increase in annual shell production is linked to the currently sharply reduced freshwater influx to the Colorado River estuary. Before the upstream river management, lower salinity retarded growth rates in these bivalves. Intra-annual growth rates were 50% lower during spring and early summer, when river flow was at its maximum. Growth rates in Chione today are largely controlled by temperature and nutrients; prior to the construction of dams and the diversion of the Colorado River flow, seasonal changes in salinity played an important role in regulating calcification rates.Our study employs sclerochronological (growth increment analysis) and geochemical techniques to assess the impact of reduced freshwater influx on bivalve growth rates in the Colorado River estuary. A combination of both techniques provides an excellent tool to evaluate the impact of river management in areas where no pre-impact studies were made.     

Esterases as biomarkers in Nereis (Hediste) diversicolor exposed to temephos and Bacillus thuringiensis var. israelensis used for mosquito control in coastal wetlands of Morbihan (Brittany,France)

Since 1998, a biomonitoring programme has been implemented to assess the potential impact of chemical mosquito control on macroinvertebrates of the coastal wetlands of Morbihan (Brittany, France). Acetylcholinesterase and carboxylesterases were used as biomarkers to assess the effects of Abate 500e (a.i. temephos) and Vectobac 12 AS (a.i. endotoxins of Bacillus thuringiensis var. israelensis, Bti) in Nereis (Hediste) diversicolor. Esterase inhibition revealed a marked impact of temephos, suggesting preferential contamination of the worms through the food. In Bti-exposed N. diversicolor, random variations of esterase activities were observed, that could not be attributed to the larvicide. However, esterases only reflected indirect physiological effects of Bti, and further investigations are needed to identify biomarkers more specific of Bti endotoxins.