Correlative changes in metabolism and DNA damage in turbot (Scophthalmus maximus) exposed to benzo[a]pyrene

Juvenile turbot (Scophthalmus maximus) were injected intraperitoneally with either corn oil or 5 mg/kg benzo[a]pyrene (BaP) dissolved in corn oil and sampled I and 3 days after injection. After 1 day, no elevation of 7-ethoxyresorufin O-deethylase (EROD) activity was observed, however bile metabolites (BaP-7,8 dihydrodiol representing 70% of the total metabolites) and a single hepatic DNA adduct spot (0.47 adducts/10(8) nucleotides) identified by 32P-postlabelling were formed. No BaP metabolites or DNA adducts were observed in either control or carrier control fish. Fish sampled after 3 days reported 5-fold higher (P < 0.05) levels of EROD activity, a shift in the bile metabolite profile towards BaP phenol formation (1OH and 30H BaP comprising up to 60% of total metabolites detected) and the formation of two adduct spots (0.86 and 0.71 adducts/10(8) nucleotides). These results show that BaP can be metabolised and form hydrophobic DNA adducts in turbot without EROD elevation. Following EROD elevation, a shift in the profile of both BaP metabolites and BaP metabolite-DNA interactions occurs indicative of other oxidative processes.     

Upper temperature tolerances of exotic brackish-water mussel, Mytilopsis leucophaeata (Conrad): an experimental study

The dark false mussel, Mytilopsis leucophaeata is an important mussel colonising the brackish-water systems of temperate and subtropical regions. Of late it has earned notoriety as a biofouling species in industrial cooling water systems. However, there are no published data on the temperature tolerance of this species. This paper presents data on the upper temperature tolerance of this mussel from the view point of biofouling control using thermal methods. In addition to mortality, response of physiological activities such as oxygen consumption, filtration rate, foot activity and byssus thread production were also studied at temperatures varying from 5 to 35 degrees C. Experiments were also carried out to understand the effect of mussel size, breeding condition, nutritional status and acclimation conditions (temperature and salinity) on the mortality pattern. The physiological activities were significantly reduced at temperatures beyond 27.5 degrees C and ceased at 35 degrees C. In 20 mm size group mussels exposed to 37 degrees C, 50% mortality was observed after 85 min and 100% mortality after 113 min. The effect of mussel size on mortality at different temperatures was significant, with the larger size group mussels showing greater resistance. M. leucophaeata collected during the non-breeding season (December-April) were more tolerant to temperature than those collected during the breeding season (June-October). Nutritional status of the mussel had no significant influence on the thermal tolerance of the mussel: fed and starved (non-fed) mussels succumbed to temperature at comparable rates. The effect of acclimation temperature and acclimation salinity on M. leucophaeata mortality at different temperatures was significant. Survival time increased with increasing acclimation temperature and decreased with increasing salinity. In comparison with other co-occurring species such as Mytilus edulis and Dreissena polymorpha, M. leucophaeata appears to be more tolerant to high temperature stress.     

Impact of grazing by benthic eukaryotic organisms on the nitrogen sediment–water exchange in the North Sea

The metabolic inhibitor cycloheximide was used to estimate the influence of primarily unicellular eukaryotes (heterotrophic protozoa) on nutrient recycling in different types of sediments in the North Sea. Fluxes of dissolved inorganic nitrogen across the sediment–water interface were measured in undisturbed sediment cores (controls) and compared to fluxes in sediment cores with cycloheximide added. If eukaryotes play an important role in nutrient recycling, one would expect to find lower nitrogen sediment–water effluxes in cores with cycloheximide due to the inactivation of eukaryotes. This important role hypothesised for eukaryotes was not generally observed: Only at four of the nineteen stations were ammonium effluxes significantly higher in controls than in cores with cycloheximide, and at five stations nitrate effluxes were significantly higher in the controls than in the cores with cycloheximide. Eukaryotic activity apparently contributed to the sediment–water exchange of ammonium through mineralisation of organic matter, nitrification and the subsequent release of ammonium and nitrate at these stations. At most other stations no differences were obtained between controls and cores with cycloheximide. This suggests that bacteria were the most important nutrient mineralisers at these stations at the time of the cruises.     

Zinc speciation in the Northeastern Atlantic Ocean

Measurements of zinc and zinc complexation by natural organic ligands in the northeastern part of the Atlantic Ocean were made using cathodic stripping voltammetry with ligand competition. Total zinc concentrations ranged from 0.3 nM in surface waters to 2 nM at 2000 m for open-ocean waters, whilst nearer the English coast, zinc concentrations reached 1.5 nM in the upper water column. In open-ocean waters zinc speciation was dominated by complexation to a natural organic ligand with conditional stability constant (log K_ZnL′) ranging between 10.0 and 10.5 and with ligand concentrations ranging between 0.4 and 2.5 nM. The ligand was found to be uniformly distributed throughout the water column even though zinc concentrations increased with depth. Organic ligand concentrations measured in this study are similar to those published for the North Pacific. However the log K_ZnL′ values for the North Atlantic are almost and order of magnitude lower than those reported by Bruland [Bruland, K.W., 1989. Complexation of zinc by natural organic-ligands in the central North Pacific. Limnol. Oceanogr., 34, 269–285.] using anodic stripping voltammetry for the North Pacific. Free zinc ion concentrations were low in open-ocean waters (6–20 pM) but are not low enough to limit growth of a typical oceanic species of phytoplankton.     

Physiological and behavioral responses of Bathynerita naticoidea (Gastropoda: Neritidae) and Methanoaricia dendrobranchiata (Polychaeta: Orbiniidae) to hypersaline conditions at a brine pool cold seep

Bathynerita naticoidea (Gastropoda: Neritidae) and Methanoaricia dendrobranchiata (Polychaeta: Orbiniidae) are two of the most abundant invertebrates associated with cold‐seep mussel beds in the Gulf of Mexico. At the methane seep known as Brine Pool NR‐1 (27 °43.415 N, 91 °16.756 W; 650 m depth), which is surrounded by a broad band of mussels (Bathymodiolus childressi), these species have distinctly different patterns of abundance, with the gastropod being found mostly at the outer edge of the mussel bed (average density in November 2003: 817 individuals·m⁻² in outer zone, 20·m⁻² in inner zone) and the polychaete being found almost exclusively near the inner edge (average density in November 2003: 3155 individuals·m⁻² in inner zone, 0·m⁻² in outer zone), adjacent to the brine pool itself. The salinity of the brine pool exceeds 120, so we hypothesized that M. dendrobranchiata should be more tolerant of high salinities than B. naticoidea. The opposite proved to be true. The gastropods were capable of withstanding salinities at least as high as 85, whereas the polychaetes died at salinities higher than 75. Both species were osmoconformers over the range of salinities (35–75) tested. Behavioral responses of B. naticoidea to salinities of 50, 60, and 70 were investigated in inverted vertical haloclines. Gastropods generally did not enter water of salinity greater than 60, but tolerated short periods at 60. Behavioral avoidance of brine should limit the vertical distribution of B. naticoidea in the inner zone to the top 2.5–5 cm of the mussel bed. Behavior is also a likely (though unproven) mechanism for controlling horizontal distribution of this species across the mussel bed. Methanoaricia dendrobranchiata can tolerate short excursions into the brine, but probably avoids hypersaline conditions by aggregating on the tops of the mussels.     

Generalized polytropes: Stellar structure with a boltzmann factor

In order to study the structure of a chemically homogeneous star in equilibrium, a density profile of the form T ^N exp(–µm(–)/kT) is suggested. As for polytropes, qualitative aspects of the resulting stellar model can be discussed analytically. In particular it is shown that one reobtains forN=3 Eddington's standard model, whereas forN<3 nearly polytropic models result. WhenN>3, the effective polytropic index does vary appreciably over the star. Numerical results indicate that the proposed density profile is quite reasonable in view of the simplicity of the model. From a comparison of the degree of precision of a polytropic approximation with that of the newly proposed model it follows that the new approximation is definitely better than the polytropic one. It is suggested that the model may be useful to study the structure of stellar clouds, clusters and (spherical) galaxies.Now at Department of Applied Mathematics, Queen Mary College, University of London, England.     

The oblateness effect on the solar radiation incident at the top of the atmospheres of the outer planets

Calculations of the daily solar radiation incident at the top of the atmospheres of Jupiter, Saturn, Uranus, and Neptune, with and without the effect of the oblateness, are presented in a series of figures illustrating the seasonal and latitudinal variation of the ratio of both insolations. It is shown that for parts of the summer, the daily insolation of an oblate planet is increased, the zone of enhanced solar radiation being strongly dependent upon the obliquity, whereas the rate of increase is fixed by both the flattening and the obliquity. In winter, the oblateness effect results in a more extensive polar region, the daily solar radiation of an oblate planet always being reduced when compared to a spherical planet. In addition, we also numerically studied the mean daily solar radiation. As previously stated by A.W. Brinkman and J. McGregor (1979, Icarus, 38, 479–482), it is found that in summer the horizon plane is tilted toward the Sun for latitudes less than the subsolar point, but is titled away from the Sun beyond this latitude. It follows that the mean summer daily insolation is increased between the equator and the subsolar point, but decreased poleward of the above-mentioned limit. In winter, however, the horizon plane is always tilted away from the Sun, causing the mean winter daily insolation to be reduced. The partial gain of the mean summertime insolation being much smaller than the loss during winter season evidently yields a mean annual daily insolation which is decreased at all latitudes.     

The stability of coronal loops: Finite-length and pressure-profile limits

Solar Physics - Vertically propagating traveling waves have been observed in the solar photosphere. These waves have a period of 278 ± 41 seconds and a vertical phase velocity of about 2 km...     

Magnetic Helicity Budget of Solar-Active Regions from the Photosphere to Magnetic Clouds

We have analyzed the long-term evolution of two active regions (ARs) from their emergence through their decay using observations from several instruments on board SoHO (MDI, EIT and LASCO) and Yohkoh/SXT. We have computed the evolution of the relative coronal magnetic helicity combining data from MDI and SXT with a linear force-free model of the coronal magnetic field. Next, we have computed the injection of helicity by surface differential rotation using MDI magnetic maps. To estimate the depletion of helicity we have counted all the CMEs of which these ARs have been the source, and we have evaluated their magnetic helicity assuming a one to one correspondence with magnetic clouds with an average helicity contain. When these three values (variation of coronal magnetic helicity, injection by differential rotation and ejection via CMEs) are compared, we find that surface differential rotation is a minor contributor to the helicity budget since CMEs carry away at least 10 times more helicity than the one differential rotation can provide. Therefore, the magnetic helicity flux needed in the global balance should come from localized photospheric motions that, at least partially, reflect the emergence of twisted flux tubes. We estimate that the total helicity carried away in CMEs can be provided by the end-to-end helicity of the flux tubes forming these ARs. Therefore, we conclude that most of the helicity ejected in CMEs is generated below the photosphere and emerges with the magnetic flux.     

Time-dependent simulations of steady C-type shocks

Using a time-dependent multifluid, magnetohydrodynamic code, we calculated the structure of steady perpendicular and oblique C-type shocks in dusty plasmas. We included relevant processes to describe mass transfer between the different fluids, radiative cooling by emission lines and grain charging, and studied the effect of single- and multiple-sized grains on the shock structure. Our models are the first of oblique fast-mode molecular shocks in which such a rigorous treatment of the dust grain dynamics has been combined with a self-consistent calculation of the thermal and ionization structures including appropriate microphysics. At low densities, the grains do not play any significant rôle in the shock dynamics. At high densities, the ionization fraction is sufficiently low that dust grains are important charge and current carriers and, thus, determine the shock structure. We find that the magnetic field in the shock front has a significant rotation out of the initial upstream plane. This is most pronounced for single-sized grains and small angles of the shock normal with the magnetic field. Our results are similar to previous studies of steady C-type shocks showing that our method is efficient, rigorous and robust. Unlike the method employed in the previous most detailed treatment of dust in steady oblique fast-mode shocks, ours allow a reliable calculation even when chemical or other conditions deviate from local statistical equilibrium. We are also able to model transient phenomena.