Development of a coral cDNA array to examine gene expression profiles in Montastraea faveolata exposed to environmental stress

The development of a cDNA array of coral genes and its application to investigate changes in coral gene expression associated with stressful conditions is described. The array includes both well-characterized and previously unidentified coral genes from Acropora cervicornis and Montastraea faveolata. Corals were exposed to either natural or anthropogenic stressors to elicit the expression of stress genes for isolation and incorporation onto the array. A total of 32 genes involved in protein synthesis, apoptosis, cell signaling, metabolism, cellular defense and inflammation were included on the array. Labeled cDNA from coral (Montastraea faveolata) exposed to elevated seawater temperature, salinity and ultraviolet light was tested against the microarray to determine patterns of gene expression associated with each stressor. Carbonic anhydrase, thioredoxin, a urokinase plasminogen activator receptor (uPAR) and three ribosomal genes demonstrated differential expression across all replicates on the array and between replicate colonies. Specific gene expression patterns produced in response to different stressors demonstrate the potential for gene expression profiling in characterizing the coral stress response.     

The northwestern Argentina Tarija Basin: Stratigraphy, depositional systems, and controlling factors in a glaciated basin

The Tarija Basin, shared by Bolivia and Argentina, was subjected to glacial conditions during the Late Carboniferous and Early Permian. The Macharetí and Mandiyutí groups deposited during these times record in their facies advances and retreats of the Gondwanan ice cap. The lithostratigraphic subdivision of these groups presents stages with minor glacial influence in the basal formations of each group (Tupambi and Escarpment formations), whereas in the upper units, glacially related deposition prevails (Tarija and San Telmo formations). Typical facies deposited in relation to glacial settings are diamictites and mudstones mainly related to proglacial, lacustrine environments. During the stages of main ice retreat, deposition was dominated by fluvial and deltaic sandstones. Significant erosion and deep valley incision characterize the basal surfaces of both groups. Conversely, the stratigraphic surface that separates the sandy formations from the overlying diamictites tends to be rather flat. The dynamics of the glacial cap are not only reflected in the facies distribution but also were a key factor in creating accommodation space; the changes in the glacial-driven subsidence linked to the advance and retreat of the ice were its main control.     

Controls on herbaceous litter decomposition in the estuarine ecotones of the Florida Everglades

The effects of nutrient availability and litter quality on litter decomposition were measured in two oligotrophic phosphorus (P)-limited Florida Everglades esturies, United States. The two estuaries differ, in that one (Shark River estuary) is directly connected to the Gulf of Mexico and receives marine P, while the other (Taylor Slough estuary) does not receive marine P because Florida Bay separates it from the Gulf of Mexico. Decomposition of three macrophytes.Cladium jamaicense, Eleochaaris spp., andJuncus roemerianus, was studied using a litter bag technique over 18 mo. Litter was exposed to three treatments: soil surface+macroinvertebrates (=macro), soil surface without macroinvertebrates (=wet), and above the soil and water (=aerial). The third treatment replicated the decomposition of standing dead leaves. Decomposition rates showed that litter exposed to the wet and macro treatments decomposed significantly faster than the aerial treatment, where atmospheric deposition was the only source of nutrients. Macroinvertebrates had no influence on litter decompostion rates.C. jamaicense decomposed faster at sites, with higher P, andEleocharis spp. decomposed significantly faster at sites with higher nitrogen (N). Initial tissue C:N and C:P molar ratios revealed that the nutrient quality of litter of bothEleocharis spp. andJ. roemerianus was higher thanC. jamaicense, but onlyEleocharis spp. decomposed faster thanC. jamaicense. C. jamaicense litter tended to immobilize P, whileEleocharis spp. litter showed net remineralization of N and P. A comparison with other estuarine and wetland systems revealed the dependence of litter decomposition on nutrient availability and litter quality. The results from this experiment suggest that Everglades restoration may have an important effect on key ecosystem processes in the estuarine ecotone of this landscape.     

Lateral moisture flow beneath a sandy hillslope without an apparent impeding layer

Part of a small drainage basin on the Sevilleta National Wildlife Refuge (about 25 km north of Socorro, NM) was intensively instrumented with soil monitoring equipment to estimate natural ground-water recharge. Soil-moisture data were analysed with special attention to characterizing the influence of topography on the direction of vadose water flow paths in fine to medium aeolian sand. Moisture content data were obtained by the neutron scattering technique, and hydraulic head data were obtained using tensiometers. In addition, tracer experiments were conducted on a sandy hillslope to delineate the flow paths of vadose water. The results indicate that there is a strong lateral component to unsaturated flow on a hillslope, even in the absence of apparent sublayers of much lower permeability. Darcian calculations estimate the long-term, steady, deep flux beneath a concave location to be about 4 per cent of an assumed mean annual precipitation of 20 cm. The deep soil water flux downward varied by several orders of magnitude during the 17 month period of record.     

Far-infrared brightness temperature of Saturn's disk and rings

We present far-infrared observations of Saturn in the wavelength band 76–116 μm, using a balloon-borne 75-cm telescope launched on 10 December 1980 from Hyderabad, India, when B′, the Saturnicentric latitude of the Sun, was 4°.3. Normalizing with respect to Jupiter, we find the average brightness temperature of the disk-ring system to be 90 ± 3° K. Correcting for the contribution from rings using experimental information on the brightness temperature of rings at 20 μm, we find T_D, the brightness temperature of the disk, to be 96.9 ± 3.5° K. The systematic errors and the correction for the ring contribution are small for our observations. We, therefore, make use of our estimate of T_D and earlier observations of Saturn when contribution from the rings was large and find that for wavelengths greater than 50 μm, there is a small reduction in the ring brightness temperature as compared to that at 20 μm.     

A search for energetic neutrons emitted during solar flares

On the basis of solar flare forecasts, balloon flights were made from Hyderabad, India (vertical geomagnetic threshold rigidity of 16.9 GV), to detect the possible emission of high energy neutrons during solar flares. The detector comprised of a central plastic scintillator, completely surrounded by an anticoincidence plastic scintillator shield. The instrument responds to neutrons of about 15–150 MeV and gamma rays of about 5–30 MeV with about the same efficiency. The detector was flown to an atmospheric depth of 25 g cm^-2 on February 26, 1969; while the balloon was at ceiling a flare of importance 2B and one of 1N occurred. No perceptible flare associated increase in the counting rate was observed. Using the observed counting rates, an upper limit of 1.2 × 10^-2 neutrons cm^-2 sec^-1 is obtained for the first time for a flare of importance 2B for neutrons of energy 15–150 MeV. The corresponding upper limit for gamma rays of energy 5–30 MeV is found to be 10^-2 photons cm^-2 sec^-1. The neutron flux limits are compared with the recent calculations of Lingenfelter.