Isotopic analysis of belemnites and brachiopods from the Cretaceous (Albian) Hunstanton Red Chalk Formation (Hunstanton,Norfolk, UK)

Cretaceous brachiopods (Moutonithyris dutempleana) and belemnites (Neohibolites minimus) from the (Albian) Hunstanton Red Chalk Formation (Hunstanton, UK) were isotopically analysed with the aim of identifying palaeoecological and palaeotemperature trends. Shell preservation was assessed via thin section petrography and geochemical analyses. Oxygen isotopic compositions (and corresponding temperature interpretations) of well-preserved belemnites are similar in comparison to the brachiopod shells. Assuming calcite precipitation in isotopic equilibrium, they are interpreted to have occupied the same or similar warm (15–19 °C) shallow marine environment. Further, these findings indicate that the belemnites mineralised in relatively warm waters and not in deep and cool waters as suggested for some belemnite species. The isotope data are thus inconsistent with the belemnites being associated with a cool water pulse, contrasting with events associated with the Cenomanian chalks. A difference δ¹³C between the belemnites and brachiopods is interpreted to originate from differences in metabolic rates.     

Toxicity assessment of New Zealand and Pacific dinoflagellates Ostreopsis and Gambierdiscus (Dinophyceae) extracts using bioassays

The dinoflagellate genus Ostreopsis produces palytoxin-like compounds (PLTXs) and the genus Gambierdiscus produces ciguatoxins (CTXs) and maitotoxin (MTX). Human illness has been recorded following the consumption of CTX-contaminated finfish. Their effects on the flora and fauna in the environment are largely unexplored. Toxin profiles of extracts of Ostreopsis and Gambierdiscus isolates from the Pacific region were characterised using liquid chromatography tandem mass spectrometry. Sea urchin (Evechinus chloriticus) fertilisation was unaffected by any algal extracts, but extracts containing maitotoxin-3 (MTX-3, but not MTX or CTXs), or with high concentrations of PLTXs, were toxic to urchin larval development. Gambierdiscus extracts containing CTXs elicited toxic responses in zebrafish (Danio rerio) embryo toxicity assays; extracts containing only MTX-3 elicited a limited response. Ostreopsis extracts containing PLTXs caused no response. The results demonstrate potential impacts of toxic dinoflagellates on larval organisms and the potential of embryonic assays as toxicity screening tools.