A fungal epizootic in mussels at a deep-sea hydrothermal vent

Mass mortalities due to disease are important determinants of population and community structure in marine ecosystems, but the speed at which an epizootic may sweep through a population, combined with rapid selection for disease‐resistant stocks, can mask the ecological impact of disease in all but the most closely monitored populations. We document an emergent epizootic event in the deep sea that is occurring in mussels (Bathymodiolus brevior) at the Mussel Hill hydrothermal vent in Fiji Basin and we identify the causal agent as a black yeast (order Chaetothyriales) that elicits a pronounced host immune response and is associated with tissue deterioration. The yeast was not observed in other invertebrate taxa (the gastropods Ifremeria nautilei, Alviniconcha aff. hessleri; the limpets Lepetodrilus schrolli, Symmetromphalus aff. hageni; the polychaetes Branchipolynoe pettiboneae, Amphisamytha cf. galapagensis) associated with the mussel bed, nor in mussels (Bathymodiolus brevior) collected from adjacent Lau Basin mussel beds. Massive mussel mortality resulting from the fungal infection is anticipated at the Mussel Hill site in Fiji Basin; we expect that epizootic outbreaks in dense invertebrate communities have the potential to be major determinants of community structure in deep‐sea chemosynthetic ecosystems. The possibility that submersible assets may serve as vectors for transport of the fungus warrants further attention.     

Characterization of the proteinaceous matter in marine aerosols

Marine aerosols play a dominant role in the transfer of oceanic material to the atmosphere. Most marine aerosol originates when air bubbles burst at the sea surface ejecting material from the sea surface microlayer and bubble surface layers into the air. Concentrations of chemical compounds in these surface layers often differ from their concentrations in bulk water. We examined the enrichment of aerosols with proteinaceous matter and attempted to characterize the physical nature and sources of this matter. We measured concentrations of dissolved free (DFAA), dissolved combined (DCAA), and particulate (PAA) amino acids, transparent stainable particles (TSP), and bacteria and virus-like particles as carriers of protein, in natural and simulated aerosols. We also evaluated D/L ratios certain amino acids in all amino acid fractions.DFAA and DCAA enriched the aerosols we sampled by 1.2–20 times compared to bulk seawater; PAA enrichment was usually higher (up to 50-fold). Aerosols contained particles typical of seawater, e.g., microorganisms, organic debris, inorganic particles with adsorbed organic matter, but also a large number of semitransparent gel-like particles, which all contained amino acids. Some of these particles were probably scavenged from bulk water, but new particles produced as bubbles burst at the surface comprised at least 10% of total proteinaceous matter in the aerosol. D/L ratios of certain amino acid suggested that the particles were most likely made from dissolved polymers secreted by phytoplankton that were concentrated on bubble surfaces and in the microlayer. Examination with Alcian Blue (a dye that targets carbohydrates) and Coomassie Blue (a dye that targets proteins) showed that most TSP in the aerosols contained both proteins and polysaccharides. Microorganisms enriched the aerosols by up to two orders of magnitude, but contributed less than 4% to the total protein pool.     

The Effect of Heavy Tamping on Structured Residual Clay Site

This paper examines the effect of heavy tamping (dynamic compaction) on highly porous structured residual clayey soil. The aim of this study is to analyse the feasibility of this technique when applied on lightly bonded residual soil sites, which are commonly found in tropical and subtropical regions. This soil has some interesting characteristics, such as high fine grain soil percentages (56% clay and 22% silt), a plastic index of 11%, high porosity (initial void ratio of 1.21), high hydraulic conductivity (about 10^?5 m/s) and a high stiffness at small strains (E?=?49.2-MPa). The research involves field [Cone Penetration Test (CPT) and the dynamic compaction] and laboratory (triaxial tests, characterization and hydraulic conductivity) investigation. According to laboratory tests, the void ratio decreased to 0.96, hydraulic conductivity decreased to 2.8?×?10^?7 m/s, the effective peak friction angle (?′) increased from 30.5° (in natural conditions) to about 35.5°, and the triaxial stiffness at small strains decreased to E?=?20-MPa due to dynamic compaction. CPT results have shown an improved depth in which CPT tip strength (q_t) increased from nearly 650-kPa to an average of 1700-kPa and CPT sleeve friction (f_s) increased from approximately 50-kPa to about 130-kPa. Horizontal displacements were observed up to about 4.0 m of depth (approximately the same depth at which CPT results showed soil improvement). It was concluded that heavy tamping reduces soil voids and substantially increases soil strength, but also breaks soil structure and decreases soil stiffness. It is thus not a suitable ground improvement solution for highly porous structured residual clayey soil.

     

The role of organic matter in the sorption capacity of marine sediments

Past studies have suggested that desiccation enhances hydrophobicity of salt marsh sediment, and that drying and rewetting sediment can be used to investigate sorption mechanisms of amino acids and other organic compounds [Liu, Z., Lee, C., 2006. Drying effects on sorption capacity of coastal sediment: The importance of architecture and polarity of organic matter. Geochim. Cosmochim. Acta 70, 3313–3324]. Here we further develop this technique to study sorption of hydrophobic and hydrophilic organic compounds in a wide range of marine sediments. Our results show that hydrophilic compounds sorb strongly to wet coastal sediments; in dried sediments, sorption of hydrophilic compounds decreases, while sorption of hydrophobic compounds is greatly enhanced. Small compounds with aromatic rings sorb more in dried than wet coastal sediments, suggesting that aromatic groups have a stronger effect on sorption than polar groups like amino and carboxyl moieties. Sorption of lysine, glutamic acid and putrescine decreases greatly when sediment is pretreated with KCl, indicating the importance of cation ion exchange. However, α-amino acids sorb much more than corresponding β- or γ-amino acids, and l-alanine sorbs more than d-alanine, suggesting that amino group location and chiral selectivity play an important role in sorption. Comparison of lysine and tyrosine sorption in different sediments indicates that source and diagenetic state of organic matter are important factors determining sorption capacity. Lysine sorbs much more to organic detritus from salt marsh sediment than to fresh Spartina root materials, marine particles, lignin or humic acids, indicating the importance of structural integrity in sorption. Desorption hysteresis of glutamic acid, putrescine and lysine (in dried sediment) suggests the presence of enzyme-type sorption sites of high sorption energy or multiple binding mechanisms. Taken together, these findings suggest that organic matter plays the major role in amino acid sorption in organic-rich sediments.     

High-resolution mean sea surface computed with altimeter data of Ers-1 (geodetic mission) and topex-poseidon

The remote-sensing satellite ERS-1, launched in 1991 to study the Earth's environment, was placed on a geodetic (168-day repeat) orbit between 1994 April and 1995 March to map, through altimetric measurements, the gravity field over the whole oceanic domain with a resolution of 8 km at the equator in both along-track and cross-track directions. We have analysed the precise altimeter data of the geodetic mission, and, by also using one year of Topex-Poseidon altimeter data, we have computed a global high-resolution mean sea surface. The various steps involved in pre-processing the ERS-1 data consisted of correcting the data for environmental factors, editing, and reducing, through crossover analyses, the radial orbit error, which directly affects sea-surface height measurements. For this purpose, we adjusted sinusoids at 1 and 2 cycle rev⁻¹ along the ERS-1 profiles in order to minimize crossover differences between ERS-1 and yearly averaged Topex-Poseidon profiles. In effect, the orbit of Topex-Poseidon is very accurately determined (within 2–3 cm for the radial component), so Topex-Poseidon altimeter profiles can serve as a reference to reduce the ERS-1 radial orbit error. The ERS-1 residual orbit error was further reduced through a second crossover analysis between all ascending and descending profiles of the geodetic mission. The along-track ERS-1 and Topex-Poseidon data were then interpolated over the whole oceanic domain on a regular grid of 1/16°× 1/16° size. The mapping of the gridded sea-surface heights reveals the very fine structure of the marine geoid, up until now unknown at a global scale. This new data set will be most useful for marine geophysical and tectonic investigations.     

Geochemistry of sterols in sediments from Black Sea and the southwest African shelf and slope

Surface sediment samples from the shelf and continental slope off southwest Africa and sediment cores from the deepest part of the Black Sea were analyzed for sterols. Because the organic matter in these anoxic sediments is relatively well-preserved, the input from source organisms in the water column is important in controlling sterol distribution patterns. The sterol distribution on the Namibian shelf is complex, probably because of the great spatial and temporal variability of biological productivity caused by seasonal upwelling and changes in oxygen concentration. The Black Sea, perhaps because of greater physical stability of the water column, has sterol distributions which can be explained by microbial activity or chemical processes acting on a constant input of organic carbon from surface production.     

Organic biomarkers in the twilight zone—Time series and settling velocity sediment traps during MedFlux

The transfer of material through the twilight zone of the ocean is controlled by sinking particles that contain organic matter (OM) and mineral ballast. During the MedFlux field program in the northwestern Mediterranean Sea in 2003, sinking particulate matter was collected in time series (TS) and settling velocity (SV) traps and analyzed for amino acids, lipids, and pigments (along with ballast minerals) [Lee, C., Armstrong, R.A., Wakeham, S.G., Peterson, M.L., Miquel, J.C., Cochran, J.K., Fowler, S.W., Hirschberg, D., Beck, A. Xue, J., 2009b. Particulate matter fluxes in time series and settling velocity sediment traps in the northwestern Mediterranean Sea. Deep-Sea Research II, this volume [doi:10.1016/j.dsr2.2008.12.003]]. The goal was to identify how organic chemical compositions of sinking particles varied as a function of their in-situ settling velocity. The TS record was used to define the biogeochemical character and temporal pattern in flux during the period of SV trap deployment. Temporal variations in organic and mineral compositions are consistent with particle biogeochemistry being driven by the seasonal succession of phytoplankton. Spring diatom bloom conditions led to a high flux of rapidly sinking aggregates and zooplankton fecal matter; summer oligotrophy followed and was characterized by a higher proportion of slowly sinking phytoplankton cells. Bacterial degradation is particularly important during the low-flux summer period. Settling velocity traps show that a large proportion of particulate organic matter sinks at 200–500 m d⁻¹. Organic compositions of this fast-sinking material mirrors that of fecal pellets and aggregated material that sinks as the spring bloom terminates. More-slowly sinking OM bears a stronger signature of bacterial degradation than do the faster-sinking particles. The observation that compositions of SV-sorted fractions are different implies that the particle field is compositionally heterogeneous over a range of settling velocities. Thus physical and biological exchange between fast-sinking and slow-sinking particles as they pass down the water column must be incomplete.     

Effects of hydrostatic pressure on microbial alteration of sinking fecal pellets

We used a new experimental device called PASS (PArticle Sinking Simulator) during MedFlux to simulate changes in in situ hydrostatic pressure that particles experience sinking from mesopelagic to bathypelagic depths. Particles, largely fecal pellets, were collected at 200 m using a settling velocity NetTrap (SV NetTrap) in Ligurian Sea in April 2006 and incubated in high-pressure bottles (HPBs) of the PASS system under both atmospheric and continuously increasing pressure conditions, simulating the pressure change experienced at a sinking rate of 200 m d⁻¹. Chemical changes over time were evaluated by measuring particulate organic carbon (POC), carbohydrates, transparent exopolymer particles (TEP), amino acids, lipids, and chloropigments, as well as dissolved organic carbon (DOC) and dissolved carbohydrates. Microbial changes were evaluated microscopically, using diamidinophenylindole (DAPI) stain for total cell counts and catalyzed reporter deposition-fluorescence in situ hybridization (CARD-FISH) for phylogenetic distinctions. Concentrations (normalized to POC) of particulate chloropigments, carbohydrates and TEP decreased under both sets of incubation conditions, although less under the increasing pressure regime than under atmospheric conditions. By contrast, dissolved carbohydrates (normalized to DOC) were higher after incubation and significantly higher under atmospheric conditions, suggesting they were produced at the expense of the particulate fraction. POC-normalized particulate wax/steryl esters increased only under pressure, suggesting biochemical responses of prokaryotes to the increasing pressure regime. The prokaryotic community initially consisted of 43% Bacteria, 12% Crenarchaea and 11% Euryarchaea. After incubation, Bacteria dominated (90%) the prokaryote community in all cases, with γ-Proteobacteria comprising the greatest fraction, followed by the Cytophaga–Flavobacter cluster and α-Proteobacteria group. Using the PASS system, we obtained chemical and microbial evidence that degradation by prokaryotes associated with fecal pellets sinking through mesopelagic waters is limited by the increasing pressure they experience.