High matrix interference affecting detection of PAH metabolites in bile of Atlantic hagfish (Myxine glutinosa) used for biomonitoring of deep-water oil production

The characteristic biology and wide distribution of hagfish species makes them relevant for use in pollution biomonitoring at great water depths, particularly in regions where deep-water oil production may take place. The exposure of fish to petrogenic contaminants can normally be detected from the level of polycyclic aromatic hydrocarbon (PAH) metabolites in bile fluid. Some of these metabolites are strong fluorophores, allowing analytical detection by means of simple fluorometric techniques such as fixed wavelength fluorescence (FF) and synchronous fluorescence scanning (SFS). In the present study bile from Atlantic hagfish (Myxine glutinosa) collected in pristine areas (Barents Sea and southwestern Norway) displayed strong bile fluorescence levels, suggesting the presence of PAH contaminants. However, gas-chromatography-mass spectrometry (GC-MS) analyses ruled out PAHs as the origin for this fluorescence signal. Rather, the bile of Myxine contains components resulting in unusually strong background fluorescence interfering at the wavelength pairs used for detection of PAH metabolites. Possible background for the observed matrix interference and implications for detection of PAH metabolites in hagfish is discussed.     

The Middle Miocene to Recent Davis Strait Drift Complex: implications for Arctic�CAtlantic water exchange

A large-scale contourite drift complex has been recognised on multi-channel 2D reflection seismic data acquired in the south-eastern Davis Strait and adjacent Labrador Sea slope offshore West Greenland between 63°?C66°N. Based on well-tie data, the drift complex developed from the Middle Miocene to the Recent. It has been mapped in a wide variety of water depths ranging from about 700?m, at a NNW-ESE-elongated crest located above structural highs in the Davis Strait, to more than 2,000?m beyond the slope to the Labrador Sea. The overall drift geometry has been described by subdivision into two first-order seismic units, enabling the generation of time-isochore maps. The reflection patterns demonstrating current-related deposition are illustrated by seismic examples. The time-isochores of the two first-order seismic units show lateral changes in their depocentres: the lower unit is absent in a zone slightly displaced south-westwards of the present-day crest, indicating changes in the prevailing deepwater current system during the Early Pliocene. The observations can be explained by two alternative palaeoceanographic scenarios: (1) either the present-day oceanographic setting with Arctic?CAtlantic water exchange across the Davis Strait was largely established by the mid-Miocene, with only minor adjustments during the Early Pliocene caused by tectonic movements, or (2) it became established during the Early Pliocene as a consequence of enhanced northward flow across the Davis Strait due to lowering of the sill depth.     

Spatial variation in river runoff into a coastal area — An ecological approach

Carbon and nitrogen stable isotopes were used to investigate spatial variation in terrestrial particulate organic matter (POM) input to a coastal area off the Tagus river estuary. Isotopic variation in higher trophic level organisms was also examined, along the coast. This study was carried out in late summer, after a period of 3 months of low river flow. The overall aim was to determine if under such conditions the coastal area is enriched by the river plume and, particularly, if lower secondary productivity should be expected in some areas. Spatial variation was detected as a gradient of decreasing terrestrial input with increasing distance from the river. It was concluded that terrestrial carbon input was also incorporated into higher trophic levels and that organisms with lower mobility are more sensitive to the gradient in terrestrial input. Even in low flow conditions the whole fishing area remained under the influence of the river plume, which still accounted for 24% of the total POM 30 km from the river mouth. Additionally, δ¹⁵N values indicated pollution input from the river Tagus.     

A straightforward approach for using single time domain simulations to assess characteristic extreme responses

Short-term wave design approach of marine structures, using nonlinear time domain simulations, is a design procedure that is recognized by various modern standard codes. One of the most challenging points of this approach is the evaluation of the characteristic extreme values for response parameters used in the design check equations. The most straightforward and recommended way to evaluate a response characteristic value is by fitting an extreme value probability distribution to the N-sample of extreme values extracted from N independent time domain simulations with duration equal to the short-term period indicated by the code, which is usually taken as 3 h. However, this procedure would not be practical for some types of marine structures, such as risers and mooring lines, under numerous design load cases and demanding huge finite element models. A more feasible approach would be to assess the response extreme value distribution using only a single short-term time domain simulation with duration shorter than 3 h. But reduced time simulations always introduce some additional statistical uncertainty into the extreme values estimates. This paper discusses a workable way of properly taking into account the statistical uncertainty associated with the simulation length in the assessment of a characteristic short-term extreme response value based on a single time series.     

红海及亚丁湾间之海水交换

分析表观耗氧量、滴定碱度及总二氧化碳量等资料来研判红海及亚丁湾间之海水交换。结果显示,红海深层水的方解石及霰石饱和度均比亚丁湾和阿拉伯海深层水的饱和度高。红海全水柱之方解石和霰石都处於过饱和状态,亚丁湾和阿拉伯海中各深度之方解石亦呈过饱和状态,但霰石的饱和探度则大约在500m左右。分析深层水之生物体无机碳与有机碳的分解比值,可以发现此地区深层水中,大约有25％的总二氧化碳增加量是由无机碳酸钙溶解而来。     

Thermal structures in the Kuroshio extension measured with a towed thermistor chain

Two-dimensional temperature data observed by use of a 275 meter towed thermistor chain deployed from an oceanographic research vessel USS MARYSVILLE, which cruised with a speed of 6.2 knots in July 1966 across the Kuroshio Extension in the North Pacific, are investigated. Two-dimensional variations of the distribution of the isotherms along the ship's track are analyzed with special reference to their slope, wavelength and wave height. The results show that the slope and wave height of isotherms have a tendency to increase as the temperature decreases. Even if the contribution of wave heights smaller than 1.5 m is neglected, i.e., contribution of large scale slope with a horizontal scale of 5–30 km is subtracted, this tendency is still detected. In contrast to this, the wavelength evaluated by the crest to crest method has no dependency on the temperature. Power spectrum of the isotherm depth is proportional tok ^–1.87 for 13°C andk ^–2.13 for 27°C, wherek is the wave number. It is shown that the spectra of warmer isotherms are relatively well approximated by –2 power law (Garrett and Munk spectrum) for internal waves rather than the –5/3 power law (Kolmogorov spectrum) for three dimensional isotropic turbulence.     

Tank wall effects on broadside current forces on barge models

Three different barge sizes were tested in uniform current in a tank of limited width. One of the barges was tested with and without bilge keels. Tests were conducted in both deep and shallow water. The barges were placed broadside to the current flow and the lateral forces on the barges were measured. The effect of the tank walls on these forces is clearly shown.     

The effects of vehicular and pedestrian traffic on dune vegetation in South Africa

The effects of varying intensities of off-road vehicle and pedestrian traffic on vegetation height and percent-cover were investigated at two sites representing pioneer and climax dune shrubland communities in South African dune systems. In all cases, a decreasing trend in vegetation height and percentage cover subsequent to application of the treatments was evident, although this was more clear in the shrub than in the pioneer community. The impact on vegetation variables increased with increasing intensity of vehicle and pedestrian treatments. Vehicles driven along a curved path resulted in greater vegetation destruction than those driven along a straight path. High intensity (single application) treatments resulted in an initial decrease in vegetation height and cover immediately after the impact, which was followed by a further decline over the next 3 months. This two-step response was attributed to the subsequent gradual death of the vegetation which was not evident immediately following the impact. Due to their faster growth rate, pioneer dune communities appear to have the capacity to recover following impacts, while dune shrub communities tend to be more vulnerable, exhibiting signs of damage for longer periods. The results of this study have value for managers of coastal dune systems, particularly those impacted by human recreational activities.     

The importance of bioturbation to continental slope sediment structure and benthic processes off Cape Hatteras, North Carolina

Even though the continental slope off Cape Hatteras has sediment accumulation rates on the order of 1 cm/year, large areas of soft sediment are intensively reworked by infaunal organisms. Primary sedimentary structures have been completely replaced with biogenic structures. Surface sedimentary structures are dominated by the bioturbational activities of a deep burrowing infauna (to at least 30 cm). The layer actively mixed by the benthos, as evidenced by sediment profile and X-ray images, is estimated to range from 5 to 20 cm, with the residence time for particles within the surface mixed layer ranging from about 4.5 to 18 years. The biological mixing parameter (G) ranges from 0.4 to 5.5, which indicates moderate to strong biological mixing relative to accumulation and strata formation. Bioturbation contributes to the dynamic forces affecting the surface sediments by decreasing compaction of sediment layers and dilating sediment fabrics by sediment mixing, and introducing large water-filled burrows and voids to subsurface sediments. The sediment profile images captured numerous subsurface feeding voids, and worms in the process of making deep burrows, many of which extended below the 5 to 10 cm average depth of the apparent color redoxpotential discontinuity layer. High rates of accumulation of organic-rich sediment lead to high standing stocks of benthos and intensive feeding/burrowing activity that result in organic rich stratagraphic sequences that are thoroughly mixed. Cape Hatteras is a apparent focusing point for the transport of shallow water sediments to the deep sea. Sediments across other areas of the continental slope just 100's of kilometers south of Cape Hatteras are not as thoroughly mixed or biologically active.     

Effects of benthic biology on bottom boundary layer processes, Dry Tortugas Bank, Florida Keys

 An instrumented tetrapod was deployed for three weeks on the Dry Tortugas Bank at a depth of 26 m in February 1995. Bottom roughness was dominated by shrimp burrows and worm mounds with rms roughness amplitudes ranging from 0.47 to 1.75 cm. Logarithmic velocity profiles show apparent total roughness heights ranging from 0.30 to 1.45 cm, values consistent with observed biological roughness. The bed sediments were weakly bound by an algal crust at the sediment–water interface. When this bound layer was scraped away by a mooring that was accidentally dragged, sharp-crested wave-induced ripples appeared within the resulting swath. We conclude that physically induced roughness is biologically suppressed, but if dominant, would be significantly higher than the prevailing biological roughness.