Synthesis and protein tyrosine phosphatase 1B inhibition activities of two new synthetic bromophenols and their methoxy derivatives

3-bromo-4,5-bis(2,3-dibromo-4,5-dihydroxybenzyl)-1,2-benzenediol (1) is a natural bromophenol isolated from the red algae Rhodomela confervoides that exhibits significant inhibition against protein tyrosine phosphatase 1B (PTP1B). Based on its activity, we synthesized two new synthetic bromophenols and their methoxy derivatives from vanillin using the structure of natural bromophenol 1 as a scaffold. The structures of these bromophenols were elucidated from 1H NMR, 13C NMR, and high resolution electron ionization mass spectrometry as 2,3-dibromo-1-(2p-bromo-6p-(3q,4q-dimethoxybenzyl)- 3p,4p-dimethoxybenzyl)-4,5-dimethoxybenzene(2),2,3-dibromo-1-(2p-bromo-6p-(2q-bromo-4q,5q-dimethoxy-benzyl)-3p,4p-dimethoxybenzyl)-4,5-dimethoxybenzene(3),3,4-dibromo-5-(2p-bromo-6p-(2q-bromo-4q,5q-dihydroxybenzyl)-3p,4p-dihydroxybenzyl)pyrocatechol(4)and 3,4-dibromo-5-(2p-bromo-6p-(3q,4q-dihydroxybenzyl)-3p,4p-dihydroxybenzyl)pyrocatechol (5).PTP1B inhibition activities of these compounds were evaluated using a colorimetric assay,and compounds 3 and 4 demonstrated interesting activity against PTP1B.     

Properties and organic activation of ocean clay in the East Pacific

In order to make use of ocean clay reasonably and excavate its potential applications the properties of ocean clay in Chinese investigating area of the East Pacific have been tested with the chemical analysis, XRFS, ICP/MAS, granulometry and SSA, etc. The results show that the clay sediments of this area are rich in clay minerals, fine granularity, bigger SSA, bigger water absorbability and lower ion changing ability etc. To improve the adaptability of ocean clay and macromolecule materials, using the dry milling method with silane, aluminate, stearic acid coupling agents etc the properties of ocean clay are improved. The effect has been evaluated by testing the water absorbing of the modified ocean clay and their dispersing in paraffin liquid. The result is that KH550 is better than any other active reagents, and the best activation technics is that 1.5% (volume) of KH550 quantity, 20min of milling time. The DTA, XRD, SEM and IR analyses and SSA testing show that the organic activation can not change the mineral structure but can densify the structure of clay, and activation mechanism is chemical covered mainly and chemical combined secondly.     

Marine bacterium strain screening and pyrethroid insecticide-degrading efficiency analysis

A pyrethroid insecticide-degrading bacterium, strain HS-24, was isolated from an offshore seawater environment. The strain, which can degrade cypermethrin(CYP) and deltamethrin(DEL), was identified as Methylophaga sp. The optimal culture and degradation conditions for CYP and DEL by strain HS-24 is pH 7 at 28°C. Under optimum culture conditions, strain HS-24 exhibited a broad degradation concentration range of 100, 200, 400, 600, and 800 mg/L for CYP and DEL. The metabolic intermediates were analyzed by NMR, which provided strong evidence that CYP and DEL removal occurred mainly because of a biological process. The toxicity of the degradation products of strain HS-24 was studied simultaneously by measuring the light output of the luminescence bacterium. This demonstrated that the biodegradation ability of strain HS-24 significantly decreased the toxicity of CYP- and DEL-contaminated aquaculture seawater. Finally, the findings of this paper indicate that strain HS-24 is thus revealed as a biological agent for the remediation of marine aquatic environments.     

Study on deformation law of circular foundation under combined loading

The foundations of some ocean engineering structures are built to withstand not only the vertical gravity load V, but also the horizontal load H induced by sea waves and current. The horizontal load includes the concentrated force load, the moment load M, and the torque load T termed also as combined loading. It is of academic and engineering significance to study the deformation law of submarine seabed due to combined loading. On the basis of the three-dimensional elastic mechanics solution of circular foundation, numerical methods are used to analyze the deformation law of submarine soil under circular foundation with six degrees of freedom. The finite element analysis results give the elastic deformation law of soil in three dimensional spaces, modify the theoretical elasticity solution, and presents nonlinear soil deformation mechanism under the circular foundation with six degrees of freedom.     

Higher species diversity occurs in more fertile habitats without fertilizer disturbance in an alpine natural grassland community

Are there some relationships among species diversity and soil chemical properties of high altitude natural grasslands？ Plant community composition and chemical properties of soil samples were compared to investigate the relationship between soil and species diversity, and the richness in Tibetan alpine grasslands. Results showed that species diversity was significantly positively related to soil organic matter （SOM）, total nitrogen （TN）, available nitrogen （AN）, total phosphorus （TP）, available phosphorus （AP）, and available potassium （AK） in the high alpine grasslands. Margalefs species richness index was also significantly positively related to SOM, TN, AN, and TP. Most soil chemical properties showed significantly positive correlation with species diversity and Margalef＇s richness index. Our results suggested that higher plant species richness index and diversity occurred in more fertile soil habitats in high altitude natural grassland community. In practice, fertilization management for the restoration of degraded grassland should be conducted with reference to the nutrient levels ofnatural grassland without the additional artificial fertilizer and with higher species-diversity and richness index.     

Dynamics and responses of vegetation to climatic variations in Ziya-Daqing basins,China

Examining the direct and indirect effects of climatic factors on vegetation growth is critical to understand the complex linkage between climate change and vegetation dynamics. Based on the Moderate Resolution Imaging Spectroradiometer(MODIS) Normalized Difference Vegetation Index(NDVI) data and meteorological data(temperature and precipitation) from 2001 to 2012, the trend of vegetation dynamics were examined in the Ziya-Daqing basins, China. The path analysis was used to obtain the information on the relationships among climatic factors and their effects on vegetation growth. It was found that the trends of growing season NDVI were insignificant in most plain dry land, while the upward trends were significant in forest, grass and dry land in Taihang Mountains. According to the path analysis, in 23% of the basins the inter-annual NDVI variation was dominated by the direct effect of precipitation, in 5% by the direct effects of precipitation and temperature, and in less than 1% by the direct effect of temperature or indirect effects of these two climatic factors. It indicated that precipitation significantly affected the vegetation growth in the whole basins, and this effect was not regulated by temperature. Precipitation increase(especially in July, August and September) was favorable to greenness enhancement. Summer temperature rising showed negative effect on plant productivity enhancement, but temperature rise in April was beneficial for the vegetation growth. When April temperature increases by 1℃, the onset date of greenness for natural vegetation will be 2 days in advance. There was a lag-time effect of precipitation or temperature on monthly NDVI for all land use types except grass.     

Climate–growth relationship stability of <Emphasis Type="Italic">Picea crassifolia</Emphasis> on an elevation gradient,Qilian Mountain,Northwest China

Climate affects Picea crassifolia growth and climate change will lead to changes in the climate–growth relationship (i.e., the “divergence” phenomenon). However, standardization methods can also change the understanding of such a relationship. We tested the stability of this relationship by considering several variables: 1) two periods (1952–1980 and 1981–2009), 2) three elevations (2700, 3000, and 3300 m), and 3) chronologies detrended using cubic splines with two different flexibilities. With increasing elevation, the climatic factor limiting the radial growth of Picea crassifolia shifted from precipitation to temperature. At the elevation of 2700 m, the relationship between radial growth and mean temperature of the previous December changed so that the more flexible spline had a greater precipitation signal. At the elevation of 3000 m, positive correlation of radial growth with mean temperature and precipitation in September of the previous year became more significant. At the elevation of 3300 m, positive correlation between radial growth and precipitation of the current summer and the previous spring and autumn was no longer significant, whereas the positive correlation between radial growth and temperature of the current spring and summer strengthened. The detrending with the most flexible spline enhanced the precipitation signal at 2700 m, while that with the least flexible spline enhanced the temperature signal at 3300 m. All results indicated that the divergence phenomenon was affected by the climatic signals in the chronologies and that it was most dependent on the detrending method. This suggests it is necessary to select a suitable spline bootstrap for studies of growth divergence phenomena.     

Extraction of basic trends of urban expansion in China over past 40 years from satellite images

If urban sprawl is to be avoided in China in the next ten years, it is not only crucial to understand the overall history, current status, and future trends of urban expansion there, but also these differences, and this is presently lacking. In this study, remotely sensed images with approximately 30 m spatial resolution were used to quantitatively assess the spatial and temporal patterns of urban expansion of 60 Chinese cities(1973–2013). Urban-expansion-process curves of the cities studied were drawn using annual expansion area as an indicator. Curve similarity analysis generated four basic process modes of urban expansion in China. These included cities that: 1) peaked around 2004 and then decelerated; 2) peaked around 2010 and then decelerated; 3) showed sustained acceleration, and 4) showed continued deceleration. Four basic process modes represented cities under different levels of development stage. Geographic location was found to be the most related characteristic to urban expansion process. Regional development policies at the national level in each region also showed highly temporal consistency with fluctuation characteristics of urban expansion process. Urban characteristic such as population size and administrative level were not found to be significantly related to urban expansion-process modes. Understanding the basic process-mode categories well is extremely important for future regional-balance planning and development of macroeconomic policies.