The impact of metals on the reproductive mechanisms of the ascidian Ciona intestinalis

Environmental pollution due to anthropogenic activities may exert an adverse impact on the reproductive mechanisms of animals. In particular, environmental chemicals introduced into seawater are able to disrupt the normal development and function of the reproductive system of marine animals. In this study, we have used the whole‐cell voltage clamp technique to examine the effects of four metals – lead, cadmium, mercury, and zinc – on reproductive mechanisms of the marine ascidian Ciona intestinalis (tunicates). In particular, we measured the effect of metals on plasma membrane electrical properties, the steady‐state conductance, the fertilization current in the mature oocyte, and larval development. Results show that oocyte voltage‐gated sodium currents are significantly reduced by all four metals, steady‐state conductance is affected only by zinc, and post‐fertilization contraction is inhibited only by lead. The fertilization current is suppressed in the presence of zinc and mercury. Embryo development up to larval stage is inhibited by zinc and mercury exposure with a reversible effect; however, a long‐term effect on larval morphology was observed. After exposure to cadmium, fertilization occurs but gives rise to abnormal larval development. These findings highlight the point that exposure to metals represents a significant risk factor for the physiology of reproduction of marine species and suggest a possible role of the C. intestinalis as a bioindicator for marine pollution monitoring.     

Mobility Patterns of Overseas Human Capital in Australia: the role of a ‘new’ graduate visa scheme and rural development policy

In 2007, the Australian federal government introduced a ‘new’ graduate visa scheme, the 485 visa sub-class. This visa facilitates the transition from student to worker for overseas graduates from Australian higher education institutions, with the potential to significantly increase their numbers in the country. At the same time, local governments have introduced various policies designed to attract and retain human capital in rural areas to address the shortfall of highly skilled individuals. This paper redresses the current lack of research into the spatial dynamics of overseas human capital in Australia and their relation to the new visa scheme in combination with shifts in rural development policies. Drawing on survey data of graduates describing the ‘before’ (2005) and ‘after’ (2008) periods of the introduction of the 485 visa scheme, this paper shows that the propensity of overseas graduates to move between Australia's regions has increased substantially. In particular, there have been an increasing proportion of overseas graduates moving to major cities despite rural development policy efforts to attract human capital to rural areas. However, these policy efforts appear to have attracted overseas graduates with particular qualifications (education and health) to work in such locales.     

Discovery of Late Jurassic fossils inside modern sediments at Gorringe Bank (Eastern Atlantic Ocean) and some geological implications.

During a recent expedition at the Gorringe Bank (eastern Atlantic, 150 miles SW off Portugal), one of the rare sites in the ocean where mantle rocks crop out at very shallow depths (? 30 m), the Gettysburg and Ormonde seamounts, the two summits on the Gorringe Bank, were surveyed in detail. At Gettysburg seamount, within the modern bioclastic material, which is continually produced on the summit and exported to deep water, several examples of Mesozoic cephalopods were found. These fossils, reworked ‘in situ’, gave an age spanning from Kimmeridgian–Tithonian to Hauterivian (145–155 Ma) and recall some condensed Jurassic sequences of the Thetyan region compatible with shallow water (< 200 m). The serendipitous discovery of such ancient faunas within modern sediments suggests that Gorringe Bank was a seamount at the early opening of the Atlantic Ocean and requires us to reassess the age of rifting along the Iberian margin and the importance of vertical tectonics for non‐volcanic, mantle‐rooted seamounts.     

An ecotoxicological protocol with caged mussels, Mytilus galloprovincialis, for monitoring the impact of an offshore platform in the Adriatic Sea

An ecotoxicological protocol with caged mussels, Mytilus galloprovincialis, was developed to evaluate the potential impact of an offshore gas platform in the central Adriatic Sea. Reference organisms were collected on a seasonal basis from an unpolluted site and transplanted for four weeks in both the sampling area and to the investigated platform. Chemical analyses of trace metals in mussel tissues were integrated with a multi-biomarker approach for the early detection of biological responses at several cellular targets. Induction of metallothioneins, peroxisomal proliferation and activity of acetylcholinesterase were measured as markers for specific classes of chemicals. Special attention was given to oxyradical metabolism and appearance of oxidative-mediated toxicity to reveal a more general onset of cellular disturbance. In addition to individual antioxidants (superoxide dismutase, catalase, glutathione S-transferases, glutathione reductase, Se-dependent and Se-independent glutathione peroxidases, and levels of total glutathione), the total oxyradical scavenging capacity (TOSC) allowed a quantification of the overall capability to neutralize specific forms of intracellular reactive oxygen species (ROS; i.e. peroxyl and hydroxyl radicals). Cellular damages were evaluated as lysosomal destabilization (membrane stability, accumulation of lipofuscin and neutral lipids), lipid peroxidation products (malondialdehyde) and DNA integrity (strand breaks and micronuclei); the air survival test was finally applied to evaluate the overall physiological condition of mussels. Concentration of trace metals (As, Ba, Cd, Cr, Cu, Fe, Hg, Mn, Ni, Pb, Zn) revealed only limited variations in transplanted mussels during various experimental periods and such changes appeared partly related to natural fluctuations. Among biological responses, variations of antioxidants and lysosomal stability were confirmed as sensitive early warning signals for biological disturbance of both natural and anthropogenic origin. The presented protocol with caged mussels allowed marked biological effects caused by the investigated platform to be excluded, and represented a useful approach that is easy to extend for monitoring the impact of offshore activities in the Adriatic sea.     

Annual dynamics of the mesozooplankton communities in a highly variable ecosystem (North Adriatic Sea,Italy)

Mesozooplankton community composition at the species/taxa level was investigated from January to December 2001, at eight stations in the northern Adriatic Sea. Annual dynamics, taxonomic composition and spatial diversity in relation to different trophic conditions are discussed as related to previous studies and to different conditions, such as the presence of mucilage events. Zooplankton communities all over the northern sub‐basin were dominated by the cladoceran Penilia avirostris in summer, and by the calanoids Paracalanus parvus, Acartia clausi and the poecilostomatoids Oncaea spp. during the rest of the year. Whereas coastal communities were more variable with time and location, we were able to identify a group of offshore stations with a similar species/taxa composition and annual dynamics. Comparing the present results with previous studies, we observed significant changes in community composition in the time scale of 20 years, mostly due to a general decrease of A. clausi as the dominant species, being replaced by P. parvus. Also, P. avirostris swarms appear to have extended their temporal occurrence, and were present for longer periods of time when compared to past records. These changes might be related to the observed general increase of the average sea water temperature in the northern Adriatic Sea.     

X‐ray computed tomography: Morphological and porosity characterization of giant Antarctic micrometeorites

Giant micrometeorites (MMs; 400–2000 µm) are exceedingly rare and scientifically valuable. Three‐dimensional nondestructive characterization by X‐ray computed tomography (X‐CT) provides information on the petrography and thus petrogenesis of MMs and serves as a guide to maximize subsequent multi‐analytical studies on such precious planetary materials. Here, we discuss the results obtained by X‐CT on 22 giant MMs and the classification based on their 3‐D density contrast images. Scoriaceous and unmelted MMs have distinct porosity ranges (10–40 vol% versus 0–25 vol%, respectively). We observe a porosity variation inside scoriaceous MMs, which allows their atmospheric entry flight history to be resolved. For the first time, spinning entry is explicitly demonstrated for four partially melted MMs. Furthermore, we are able to resolve the thermal gradient in a single particle, based on porosity variation (seen as a progressive increase in pore abundance and size with higher peak temperatures). Moreover, we explore parent body alteration through the 3‐D analysis of pores distribution, showing that shock fabrics are either absent or weakly developed in our data set. Finally, owing to the detection of pseudomorphic chondrules, we estimate that the intensively aqueously altered C1 or CI‐like material could represent 18% of the MM flux at this size fraction (400–1000 µm).     

Seismic response prediction of reinforced concrete buildings through nonlinear combinations of intensity measures

A widespread approach for the prediction of the structural response as function of the ground motion intensity is based on the Cloud Analysis: once a set of points representing the engineering demand parameter (EDP) values is obtained as function of the selected seismic intensity measure (IM) for a collection of unscaled earthquake records, a regression analysis is performed by assuming a specific functional form to correlate these variables. Within this framework, many studies have been devoted so far to evaluate the effectiveness of several IMs in estimating the EDPs through intrinsically linear functional forms, but it is still unknown to what extent the use of the linear regression analysis affects the quality of the final results. This paper is intended to provide an answer to such question by means of the calibration of suitable nonlinear combinations of scalar IMs, whose statistical performances are compared with those obtained by using the functional form usually adopted for linear regression-based calibrations. Specifically, the Evolutionary Polynomial Regression technique is adopted to calibrate nonlinear regression models for the prediction of maximum inter-story drift ratio and maximum floor acceleration. The comparative analysis is performed for fixed-base and base-isolated reinforced concrete buildings subjected to ordinary or pulse-like ground motion taking into account accuracy, complexity, efficiency and sufficiency. Final results demonstrate that the linear regression analysis is suitable for fixed-base reinforced concrete buildings, but nonlinear regression models provide better estimates. On the other hand, the linear regression analysis can introduce a significant bias in the seismic response prediction of base-isolated buildings, and nonlinear regression models are deemed more appropriate.     

New coring study in Augusta Bay expands understanding of offshore tsunami deposits (Eastern Sicily,Italy)

Tsunami deposits present an important archive for understanding tsunami histories and dynamics. Most research in this field has focused on onshore preserved remains, while the offshore deposits have received less attention. In 2009, during a coring campaign with the Italian Navy Magnaghi, four 1 m long gravity cores (MG cores) were sampled from the northern part of Augusta Bay, along a transect in 60 to 110 m water depth. These cores were taken in the same area where a core (MS06) was collected in 2007 about 2·3 km offshore Augusta at a water depth of 72 m below sea level. Core MS06 consisted of a 6·7 m long sequence that included 12 anomalous intervals interpreted as the primary effect of tsunami backwash waves in the last 4500 years. In this study, tsunami deposits were identified, based on sedimentology and displaced benthic foraminifera (as for core MS06) reinforced by X-ray fluorescence data. Two erosional surfaces (L1 and L2) were recognized coupled with grain-size increase, abundant Posidonia oceanica seagrass remains and a significant amount of Nubecularia lucifuga, an epiphytic sessile benthic foraminifera considered to be transported from the inner shelf. The occurrence of Ti/Ca and Ti/Sr increments, coinciding with peaks in organic matter (Mo incoherent/coherent) suggests terrestrial run-off coupled with an input of organic matter. The L1 and L2 horizons were attributed to two distinct historical tsunamis (ad 1542 and ad 1693) by indirect age-estimation methods using ²¹⁰Pb profiles and the comparison of Volume Magnetic Susceptibility data between MG cores and MS06 cores. One most recent bioturbated horizon (Bh), despite not matching the above listed interpretative features, recorded an important palaeoenvironmental change that may correspond to the ad 1908 tsunami. These findings reinforce the value of offshore sediment records as an underutilized resource for the identification of past tsunamis.