Depuration dynamics of oysters (Crassostrea gigas) artificially contaminated by Salmonella enterica serovar Typhimurium

The State of Santa Catarina produces the greatest quantity of edible mollusks in Brazil. To guarantee sanitary qualify, mollusk cultures should be monitored for contamination by pathogenic microorganisms. A self-purification or "depuration" system that eliminates Salmonella enterica serovar Typhimurium contamination from oysters has been developed and evaluated. The depuration process occurred within a closed system, in which 1000 L of water was recirculated for 24 h. The water was sterilized with ultraviolet (UV) light, chlorine, or both together. Oysters (Crassostrea gigas) artificially contaminated with S. typhimurium were harvested every 6 h. Samples of oyster tissue were excised and both the presence and numbers of bacteria were determined. Combined UV light and chlorine treatments resulted in total elimination of bacteria within 12 h. Polymerase chain reaction detected bacteria in water exposed to the three treatments. This pioneering study is the first of its kind in Brazil and represents a major contribution to commercial mollusk culture in this country.     

Using helicopter electromagnetic data to predict groundwater quality in fractured crystalline bedrock in a semi-arid region, Northeast Brazil

Geostatistical modeling, using airborne and borehole electromagnetic data, was used to estimate electrical conductivity in groundwater within fractured paragneisses and migmatites in a semi-arid climate in northeastern Brazil. Despite the geologic heterogeneity of crystalline aquifers, the use of high resolution helicopter electromagnetic (HEM) data enabled the characterization of groundwater electrical conductivity where data from drilled wells were insufficient. The tacit assumption is that HEM measurements can be used to relate rock electrical conductivity to groundwater electrical conductivity. In this study, the HEM data were used as an external drift variable in non-stationary estimation and stochastic simulation to identify the variability of groundwater electrical conductivity. Validation tests, comparing predicted values for groundwater conductivity with measurements in new wells, confirmed the success of these models in locating fresh groundwater sources in crystalline bedrock.     

Inorganic nitrogen dynamics in intertidal rocky biofilms and sediments of the Douro River estuary (Portugal)

In this study rates of oxygen, ammonium (NH₄ ⁺), nitrate (NO₃ ⁻), nitrite (NO₂ ⁻), and nitrous oxide (N₂O) fluxes, nitrogen (N) fixation, nitrification, and denitrification were compared between two intertidal sites for which there is an abundant global literature, muddy and sandy sediments, and two sites representing the rocky intertidal zone where biogeochemical processes have scarcely been investigated. In almost all sites oxygen production rates greatly exceeded oxygen consumption rates. During daylight, NH₄ ⁺ and NO₃ ⁻ uptake rates together with ammonification could supply the different N requirements of the primary producer communities at all four sites; N assimilation by benthic or epilithic primary producers was the major process of dissolved inorganic nitrogen (DIN) removal; N fixation, nitrification, and denitrification were minor processes in the overall light DIN cycle. At night, distinct DIN cycling processes took place in the four environments, denitrification rates ranged from 9 ± 2 to 360 ± 30 μmol N₂ m⁻² h⁻¹, accounting for 10–48% of the water column NO₃ ⁻ uptake; nitrification rates varied from 0 to 1712 ± 666 μmol NH₄ ⁺ m⁻² h⁻¹. A conceptual model of N cycle dynamics showed major differences between intertidal sediment and rocky sites in terms of the mean rates of DIN net fluxes and the processes involved, with rocky biofilm showing generally higher fluxes. Of particular significance, the intertidal rocky biofilms released 10 times the amount of N₂O produced in intertidal sediments (up to 17 ± 6 μmol N₂O m⁻² h⁻¹), representing the highest N₂O release rates ever recorded for marine systems. The biogeochemical contributions of intertidal rocky substrata to estuarine and coastal processes warrant future detailed investigation.     

Study of the macrophyte assemblages and application of phytobenthic indices to assess the Ecological Status of the Marano‐Grado Lagoon (Italy)

Benthic macrophytes from 19 sites within the Marano‐Grado Lagoon were sampled in July 2007 in order to update the available information on the flora and vegetation and to assess the Ecological Status (ES) within the European Water Framework Directive (WFD). Data on macrophytes were analysed using two phytobenthic indices (EEI and R‐MaQI) recently set up to evaluate the ecological status of transitional environments in the Mediterranean eco‐region. Notwithstanding the extension (c. 160 km²) that places this lagoon as the second largest Italian transitional environment, ecological studies are relatively few. The present research revealed a relatively poor flora, mainly characterized by the dominance of low‐diversity settlements of Ulvaceae. Moreover, the absence of intensive aquaculture activities and commercial big harbours, which account for the new species introductions recorded in other lagoons, limits the presence of non‐autochthonous species. The comparison with previous data ( Curiel et al. 1998 ) highlighted the reduction of macrophyte coverage and biomass, especially Ulvaceae stands, and an increase in species richness. In particular, there was evidence of a worsening of the area north of the Grado bridge. This area, which in the past was colonized by widespread angiosperm coverage, at present is almost lacking in vegetation. According to these observations, by applying both the phytobenthic indices available in the literature for the transitional environments, the Marano‐Grado Lagoon showed a ‘Good–High’ quality in the central part of the basin and near the lagoon inlets and a ‘Poor–Bad’ quality in the northern and southern areas. The evaluation of some physico‐chemical parameters, coupled with the distribution of the angiosperms, also allowed a first delineation of the main lagoon water bodies.     

Strike-slip faulting as a trigger mechanism for overpressure release through piercement structures. Implications for the Lusi mud volcano,Indonesia

Piercement structures such as hydrothermal vent complexes, pockmarks, and mud volcanoes, are found in various geological settings but are often associated with faults or other fluid-focussing features. This article aims to investigate and understand the mechanisms responsible for the formation of piercement structures in sedimentary basins and the role of strike-slip faulting as a triggering mechanism for fluidization. For this purpose four different approaches were combined: fieldwork, analogue experiments, and mathematical modeling for brittle and ductile rheologies. The results of this study may be applied to several geological settings, including the newly formed Lusi mud volcano in Indonesia (Mazzini et al., 2007).     

Environmental control on early life stages of flatfishes in the Lima Estuary (NW Portugal)

Several flatfishes spawn in oceanic waters and pelagic larvae are transported inshore to settle in the nursery areas, usually estuaries, where they remain during their juvenile life. Nursery areas appear as extremely important habitats, not only for juveniles but also for the earlier planktonic larval fish. Yet, the majority of nursery studies tend to focus only on one development stage, missing an integrative approach of the entire early life that fishes spent within a nursery ground. Thus, the present study assessed the influence of environmental parameters on the dynamics of the larval and juvenile flatfishes, throughout their nursery life in the Lima Estuary. Between April 2002 and April 2004, fortnightly subsurface ichthyoplankton samples were collected and juveniles were collected from October 2003 until September 2005. Larval assemblages comprised nine flatfish species, while only six were observed among the juvenile assemblages. Solea senegalensis and Platichthys flesus were the most abundant species of both fractions of the Lima Estuary flatfishes. Larval flatfish assemblages varied seasonally, without relevant differences between lower and middle estuary. Platichthys flesus dominated the spring samples and summer and autumn periods were characterized by an increase of overall abundance and diversity of larval flatfishes, mainly S. senegalensis, associated with temperature increase and reduced river flow. On the contrary, during the winter abundance sharply decreased, as a consequence of higher river run-off that might compromised the immigration of incompetent marine larvae. Juvenile flatfishes were more abundant in the middle and upper areas of the estuary, but the species richness was higher near the river mouth. Sediment type, distance from the river mouth, salinity, temperature and dissolved oxygen were identified as the main environmental factors structuring the juvenile flatfish assemblages. Juveniles were spatially discrete, with the most abundant species S. senegalensis and P. flesus associated with the middle and upper estuary, while the remaining species were associated with the lower estuarine areas. The larval fraction exhibited distinct dynamics from the juvenile estuarine flatfish community. Larval flatfishes showed a strong seasonal structure mainly regulated by biological features as the spawning season and also by seasonal variations of water characteristics. On the other hand, juvenile flatfishes were markedly controlled by site specific characteristics such as sediments structure, distance from the river mouth and salinity regime. The present study emphasized the idea that the environmental control varies throughout the ontogenetic development, stressing the importance of integrating all the early life of a species in flatfish nursery studies.     

Recruitment of flatfish species to an estuarine nursery habitat (Lima estuary, NW Iberian Peninsula)

One of the present concerns of fish biologists involves defining and identifying nursery habitats in the context of conservation and resource management strategies. Fish nursery studies usually report upon nursery occupation during the latter juvenile stages, despite the fact that recruitment to nurseries can start early in life, during the larval phase. Here we investigated the use of a temperate estuarine nursery area, the Lima estuary (NW Portugal), by initial development stages of flatfish species before and after metamorphosis, integrating the larval and juvenile phases. The Lima estuarine flatfish community comprised twelve taxa, seven of which were present as pelagic larvae, six as juveniles and three as adults. There was a general trend of increasing spring–summer abundance of both larvae and juveniles, followed by a sharp winter decrease, mainly of larval flatfishes. The Lima estuary was used by Solea senegalensis, Platichthys flesus and Solea solea as a nursery area, with direct settlement for the two first species. In contrast, indirect settlement was suggested for S. solea, with metamorphosis occurring outside the estuarine area. Estuarine recruitment of S. senegalensis varied between years, with young larvae occurring in the estuary throughout a prolonged period that lasted 6–9 months, corroborating the protracted spawning season. P. flesus, the second most abundant species, exhibited a typical spring estuarine recruitment, without inter-annual variations. Developed larvae arrived in the estuary during spring, whereas the 0-group juveniles emerged in the following summer period. The present study contributes new insight to our understanding of the economically important S. senegalensis, and highlights the importance of integrating the planktonic larval phase into traditional flatfish nursery studies.     

Impact of projected climate change on hydrologic regime of the Upper Paraguay River basin

We present an assessment of climate change impacts on the hydrologic regime of the 600,000 km² Upper Paraguay River basin, located in central South America based on predictions of 20 Atmospheric/Ocean General Circulation Models (AOGCMs). We considered two climate change scenarios from the Intergovernmental Panel on Climate Change (IPCC) and two 30-years time intervals centered at 2030 and 2070. Projected temperature and precipitation anomalies estimated by the AOGCMs for the study site are spatially downscaled. Time series of projected temperature and precipitation were estimated using the delta change approach. These time series were used as input to a detailed coupled hydrologic-hydraulic model aiming to estimate projected streamflow in climate change scenarios at several control points in the basin. Results show that impacts on streamflow are highly dependent on the AOGCM used to obtain the climate predictions. Patterns of temperature increase persist over the entire year for almost all AOGCMs resulting in an increase in the evapotranspiration rate of the hydrological model. The precipitation anomalies show large dispersion, being projected as either an increase or decrease in precipitation rates. Based on these inputs, results from the coupled hydrologic-hydraulic model show nearly one half of projections as increasing river discharge, and other half as decreasing river discharge. If the mean or median of the predictions is considered, no discernible change in river discharge should be expected, despite the dispersion among results of the AOGCMs that reached +/?10 % in the short horizon and +/? 20 % in the long horizon, at several control points.     

L1 norm inversion method for deconvolution in attenuating media

In order to perform a good pulse compression, the conventional spike deconvolution method requires that the wavelet is stationary. However, this requirement is never reached since the seismic wave always suffers high‐frequency attenuation and dispersion as it propagates in real materials. Due to this issue, the data need to pass through some kind of inverse‐Q filter. Most methods attempt to correct the attenuation effect by applying greater gains for high‐frequency components of the signal. The problem with this procedure is that it generally boosts high‐frequency noise. In order to deal with this problem, we present a new inversion method designed to estimate the reflectivity function in attenuating media. The key feature of the proposed method is the use of the least absolute error (L1 norm) to define both the data and model error in the objective functional. The L1 norm is more immune to noise when compared to the usual L2 one, especially when the data are contaminated by discrepant sample values. It also favours sparse reflectivity when used to define the model error in regularization of the inverse problem and also increases the resolution, since an efficient pulse compression is attained. Tests on synthetic and real data demonstrate the efficacy of the method in raising the resolution of the seismic signal without boosting its noise component.