Microencapsulation of okadaic acid as a tool for studying the accumulation of DSP toxins in mussels

The possibility and effectiveness of microencapsulation of okadaic acid (OA) in gelatin-acacia microcapsules has been studied. The encapsulation efficiency was higher than 33%. The microcapsules were shown to be very stable, not leaching more than 9% of the encapsulated OA in a 20-h period. OA from the microcapsules was absorbed by the mussels very efficiently, accumulating--after 3 days of feeding and one of depuration--65% of the OA in microcapsules and 22% of the total OA used at the beginning of the microencapsulation process. These efficiencies and the possibility of encapsulating single DSP toxins and derivatives constitute a valuable tool for the study of the accumulation and biotransformation of DSP toxins in bivalves.     

Morphogenesis of the SW Balearic continental slope and adjacent abyssal plain,Western Mediterranean Sea

We present the seafloor morphology and shallow seismic structure of the continental slope south-east of the Balearic promontory and of the adjacent Algero-Balearic abyssal plain from multibeam and chirp sonar data. The main purpose of this research was to identify the sediment pathways from the Balearic promontory to the Algero-Balearic deep basin from the Early Pliocene to the Present. The morphology of the southern Balearic margin is controlled by a SW–NE structural trend, whose main expressions are the Emile Baudot Escarpment transform fault, and a newly discovered WSW–ENE trend that affects the SW end of the escarpment and the abyssal plain. We relate the two structural trends to right-lateral simple shear as a consequence of the Miocene westward migration of the Gibraltar Arc. Newly discovered steep and narrow volcanic ridges were probably enabled to grow by local transtension along the transform margin. Abyssal plain knolls and seahills relate to the subsurface deformation of early stage halokinetic structures such as salt rollers, salt anticlines, and salt pillows. The limited thickness of the overburden and the limited amount of deformation in the deep basin prevent the formation of more mature halokinetic structures such as diapirs, salt walls, bulbs, and salt extrusions. The uppermost sediment cover is affected by a dense pattern of sub-vertical small throw normal faults resulting from extensional stress induced in the overburden by subsurface salt deformation structures. Shallow gas seismic character and the possible presence of an active polygonal fault system suggest upward fluid migration and fluid and sediment expulsion at the seafloor through a probable mud volcano and other piercement structures. One large debris flow deposit, named Formentera Debris Flow, has been identified on the lower slope and rise of the south Formentera margin. Based on current observations, we hypothesize that the landslide originating the Formentera Debris Flow occurred in the Holocene, perhaps in historical times.

ADM1‐Based Robust Interval Observer for Anaerobic Digestion Processes

A robust state estimation scheme is proposed for anaerobic digestion (AD) processes to estimate key variables under the most uncertain scenarios (namely, uncertainties on the process inputs and unknown reaction and specific growth rates). This scheme combines the use of the IWA Anaerobic Digestion Model No. 1 (ADM1), the interval observer theory and a minimum number of measurements to reconstruct the unmeasured process variables within guaranteed lower and upper bounds in which they evolve. The performance of this robust estimation scheme is evaluated via numerical simulations that are carried out under actual operating conditions. It is shown that under some structural and operational conditions, the proposed robust interval observer (RIO) has the property of remaining stable in the face of uncertain process inputs, badly known kinetics and load disturbances. It is also shown that the RIO is indeed a powerful tool for the estimation of biomass (composed of seven different species) from a minimum number of measurements in a system with a total of 32 variables from which 24 correspond to state variables.     

Trace metals in tissues of gray whale (Eschrichtius robustus) carcasses from the Northern Pacific Mexican Coast

Samples of liver, lung, heart, muscle, and blubber tissue from the carcasses of juvenile gray whales (Eschrichtius robustus) found stranded on the coast off the Sea of Cortez, México were analyzed for a range of trace metals (Cu, Fe, Zn, Mn, Ni, Pb, and Cd). The highest concentrations of copper, iron, zinc, and manganese were found in liver; nickel and lead in heart, and cadmium in kidney. In all tissues analyzed, iron, zinc and copper were present in the highest concentrations; however, some whales also showed high levels of cadmium in the kidney which could be related to their diet. Elevated concentrations of copper were found only in the liver of one whale. In general, where low levels of iron were found in the liver, they were associated with poor nutrition. Lead, nickel, manganese and zinc levels in liver were within the normal range, indicating that these whales had not been exposed to high levels of these metals.     

The current tectonic motion of the Northern Andes along the Algeciras Fault System in SW Colombia

Riedel, synthetic and antithetic type faults, principal displacement zones (PDZ), pull-apart basins (such as lazy-S shaped releasing bend, extensive and rhomboidal shaped and releasing sidestep basins) and minor folds located oblique to the main trace of the Algeciras Fault System (AFS) are interpreted from Landsat TM 5 images and geological mapping. These tectonic features are affecting Quaternary deposits and are related to major historical earthquakes and recent registered seismic events, indicating neotectonic activity of the structure.The AFS is classified as a right lateral wrench complex structure, with an important vertical component in which sedimentary cover and basement rocks are involved. In addition, the system represents a simple shear caused by the oblique convergence between the Nazca Plate and the northern Andes. The transpressive boundary in SW Colombia was previously located along the Eastern Frontal Fault System. However, this paper shows that the AFS constitutes the actual boundary of the current transpressive regime along the Northern Andes, which begins at the Gulf of Guayaquil in Ecuador and continues into Colombia and Venezuela.     

Spatial discordance in fish,coral, and sponge assemblages across a Caribbean atoll reef gradient

Certain biodiversity patterns on coral reefs are generally consistent but we still lack fundamental insight into how assemblages vary across spatially heterogeneous reef systems. We compared fish, coral, and sponge assemblages across a symmetrical physiographical gradient (windward forereef, lagoon patch reef, leeward forereef) of the Glover's Reef atoll, Belize. Species richness of fishes and corals was highest in the deep habitat (15 m) on the windward forereef. Sponges were diverse and abundant on both deep windward and leeward forereefs but not on the exposed shallow (5 m) windward forereef. Fish and benthic assemblages were relatively distinct in each reef zone, with the lagoon patch reef communities consisting of a combination of leeward and windward species. Nevertheless, there were no clear patterns in community similarity matrices of fish and benthic assemblages, suggesting that overall coral and sponge assemblages had weak or no direct association with patterns in fish assemblages. A closer examination of fish trophic groups indicated that planktivores and predators were predictably associated with depth, whereas herbivores were associated with shallow protected reefs. None was specifically associated with spatial location along the atoll gradient. These patterns of diversity distribution are important for identifying spatial conservation priorities. A Marine Protected Area (MPA) at Glover's Reef encompasses substantial windward forereef and patch reef habitats. A much lesser extent of protection is afforded the leeward forereef that supports faunal assemblages that are unique and productive, if not as diverse as the windward forereef. Isolated coral atolls can serve as ideal systems to study spatial heterogeneity and biodiversity patterns, but more experimental studies are needed to reveal the mechanistic processes underlying these patterns.     

Metals and metalloids in primary gold mining districts of Western Bolivia: anthropogenic and natural sources

Topsoil and subsoil samples located adjacent and distant from the mining operations sites were collected. Most total metals showed no significant differences between topsoil and subsoil or proximity, suggesting that they derive from endogenous parent material. There was an increment in Hg in topsoils adjacent to the mining operation sites, indicating a deposition of Hg from the amalgamation areas. Higher values of total, extractable and soluble As were observed adjacent to the mining operation sites, probably related to the presence of residues, rich in arsenopyrite. Organic matter and clay contents control the concentrations of EDTA-extractable Cd and Zn, while soil acidity was associated with the behaviour of As, Hg and Cu. In contrast the concentration of EDTA-extractable Pb was directly affected by its total concentration. In general, soluble metals were highly independent, without significant correlations with any soil physical and chemical properties.