Pathological reactions and recovery of hepatopancreatic digestive cells from the marine snail Littorina littorea following exposure to a polycyclic aromatic hydrocarbon

The aim of this study was to investigate the cellular pathological responses of hepatopancreatic digestive cells from the periwinkle Littorina littorea exposed to the polycyclic aromatic hydrocarbon (PAH) fluoranthene and to ascertain whether any injurious effects were reversible within the experimental time scale. A secondary objective was to establish the relationship of the various reactions to animal health status, using lysosomal stability as an index of well-being. Exposure of snails to a concentration of 335 microgl(-1) (1.7 microM) fluoranthene (seawater renewed and spiked daily with fluoranthene) for 5 days resulted in a reduction in lysosomal stability (neutral red retention) and endocytosis; and an increase in smooth endoplasmic reticulum (ER) and 7-ethoxycoumarin-o-deethylase (ECOD; measured as cyano-ECOD) activity measured in isolated live digestive cells. Exposed snails treated with clean seawater for a further 8 days resulted in a return to control levels of lysosomal stability, ECOD and ER; endocytosis showed only a partial recovery. Multi-variate and uni-variate analysis showed that there were strong correlations between the various cellular biomarker responses. These findings are interpretable within the current framework of molluscan biomarker responses to PAHs. Principal component analysis was used to derive the first principal component for endocytosis, ER and ECOD reactions and these were plotted against lysosomal stability as a measure of cellular well-being. The resulting significant regression represents the mapping of the individual biomarkers within health status space for a gradient of fluoranthene toxicity. From this analysis, we concluded that endocytosis is an indicator of healthy snails while proliferation of ER and to a lesser extent induced ECOD are indicative of dysfunction and reduced health. Finally, the results indicate that stress induced by chronic exposure to a PAH is reversible.     

Impact of the Sea Empress oil spill on lysosomal stability in mussel blood cells.

Coastal zones are among the most productive and vulnerable areas on the planet. An example of impact on these fragile environments was shown in the case of the "Sea Empress" oil tanker, which ran aground in the Bristol Channel in 1996, spilling 72,000 tons of "Forties" crude oil. The objective was to investigate the sub-lethal cellular pathology and tissue hydrocarbon contamination in marine mussel populations, 4 months after the initial spill, using the neutral red retention (NRR) assay for lysosomal stability in blood cells. NRR was reduced in mussels, and indicative of cell injury, from the two sites closest to the spill in comparison with more distant and reference sites. Lysosomal stability was inversely correlated with polycyclic aromatic hydrocarbon concentrations in mussel tissues. Reduced lysosomal stability has previously been shown to contribute to impaired immunocompetence and to autophagic loss of body tissues. The use of this type of technique is discussed in the context of cost-effective, ecotoxicological tools for Integrated Coastal Zone Management.     

Stratigraphic evolution of a submarine channel-lobe complex system in a narrow fairway within the Magallanes foreland basin, Cerro Toro Formation, southern Chile

This study integrates newly acquired stratigraphic data, geologic mapping, and paleocurrent data to constrain the stratigraphic evolution of the oldest channel-lobe complex in the Upper Cretaceous Cerro Toro Formation in the Silla Syncline area of the Magallanes Basin, termed the Pehoe member. The Pehoe member ranges in thickness from 60 m in the north to at least 410 m farther down system and comprises three separate divisions (A, B, and C). A lower conglomerate unit and an upper one, termed Pehoe A and C divisions respectively, represent the fill of major incised submarine channels or channel complexes. These are separated by stratified sandstone of the Pehoe B division, representing a weakly confined lobe complex, either transient or terminal.The integration of new data with observations from previous studies reveal that the three main coarse-grained conglomerate and sandstone members in the Cerro Toro Formation in the Silla Syncline include at least seven distinct submarine channels or channel complexes and two major lobe complexes. The thinning and disappearance of these units along the eastern limb of the syncline reflect confinement of the flows to a narrow trough or mini-basin bounded to the east by a topographic high. This confinement resulted in unidirectional paleocurrents to the south and southeast in all deposits. Changes in depositional geometries are interpreted as reflecting changes in sediment supply and relative confinement. Submarine channels were from 700 m to 3.5 km wide and occupied a fairway that was 4-5 km wide. Flows moving south and southeast in this mini-basin probably crossed the eastern topographic high south of the present exposures and joined those moving southward along the axis of the foreland basin at least 16 km to the east.     

A computer-aided framework for subsurface identification of white shark pigment patterns

Subsurface video footage can be used as a successful identification tool for various marine organisms; however, processing of such information has proven challenging. This study tests the use of automated software to assist with photo-identification of the great white shark Carcharodon carcharias in the region of Gansbaai, on the south coast of South Africa. A subsurface photo catalogue was created from underwater video footage. Single individuals were identified by using pigmentation patterns. From this catalogue, two images of the head for each individual were inserted into automated contour-recognition software (Interactive Individual Identification System Beta Contour 3.0). One image was used to search the database, the other served as a reference image. Identification was made by means of a contour, assigned using the software to the irregular border of grey and white on the shark's head. In total, 90 different contours were processed. The output provided ranks, where the first match would be a direct identification of the individual. The method proved to be accurate, in particular for high-quality images where 88.24% and 94.12%, respectively, were identified by two independent analysts as first match, and with all individuals identified within the top 10 matches. The inclusion of metadata improved accuracy and precision, allowing identification of even low-quality images.     

Ecosystem management bioindicators: the ECOMAN project – a multi-biomarker approach to ecosystem management

The ECOMAN project was initiated from an awareness of the complexity of the functioning of coastal marine systems and the clear need for more pragmatic environmental assessment techniques linking environmental degradation with its causes. The aim of the project is to develop a suite of easy to use, cost effective and environmentally valid biological responses (biomarkers) to assess the general health of coastal systems, including estuaries. To achieve this aim, various sublethal endpoints are being measured and evaluated from a range of common coastal organisms showing different feeding types (filter feeding, grazing and predation) and habitat requirements (estuary and rocky shore) and at different levels of biological response (cellular, physiological and behavioural). This holistic integrated approach is essential to identify the full impact of chemical contamination on organisms, and enables the sensitivity of organisms to be ranked and key sentinel species for specific habitats to be identified.     

Beyond the spectacle of property windfalls in Singapore: Lefebvrian spaces of home against profit-making

In Singapore, owners of private housing estates reaping windfalls through collective sales have been subject to the news media's sensational coverage of the topic. While collective sales will give rise to new developments that regenerate the country's landscape to reflect the image and identity of an entrepreneurial city, efforts to resist this wealth generating urbanism are subject to erasure. Instances of minority owners disinterested in profit-making using the courts to save their homes from the collective sale juggernaut are certainly emblematic of the inherent tension between one's home as housing and home as investment repository. Drawing upon ethnographical experiences from a housing estate built for public officials in Singapore, this article's use of the Lefebvrian theory of spatial production sheds light on how minority owners unmotivated by monetary windfalls (re)produce spaces of their homes that are overtly inimical to the impulses of majority owners to monetize their homes at high premiums.     

Subdividing the Holocene Series/Epoch: formalization of stages/ages and subseries/subepochs,and designation of GSSPs and auxiliary stratotypes

The Holocene, which currently spans ~11 700 years, is the shortest series/epoch within the geological time scale (GTS), yet it contains a rich archive of evidence in stratigraphical contexts that are frequently continuous and often preserved at high levels of resolution. On 14 June 2018, the Executive Committee of the International Union of Geological Sciences formally ratified a proposal to subdivide the Holocene into three stages/ages, along with their equivalent subseries/subepochs, each anchored by a Global boundary Stratotype Section and Point (GSSP). The new stages are the Greenlandian (Lower/Early Holocene Subseries/Subepoch) with its GSSP in the Greenland NGRIP2 ice core and dated at 11 700 a b2k (before 2000 CE); the Northgrippian (Middle Holocene Subseries/Subepoch) with its GSSP in the Greenland NGRIP1 ice core and dated at 8236 a b2k; and the Meghalayan (Upper/Late Holocene Subseries/Subepoch) with its GSSP in a speleothem from Mawmluh Cave, north‐eastern India, with a date of 4250 a b2k. We explain the nomenclature of the new divisions, describe the procedures involved in the ratification process, designate auxiliary stratotypes to support the GSSPs and consider the implications of the subdivision for defining the Anthropocene as a new unit within the GTS.