土地开发整理项目管理所存在的问题与解决方案

近年来,国家高度重视土地整治工作,在云南省启动了?“兴地睦边”农田整治重大工程项目。云南省委、省政府专门成立了中低产田改造领导机构,计划用十年时间,完成全省2000万亩中低产田的改造任务。     

Environmental Isotopic Characterization of Groundwater and Surface Water in Northeast Missan Province,South Iraq

The present work studies the environmental isotopes assess groundwater characteristics of the different parts of the main aquifer in the northeast Missan Province in south of Iraq.Water samples of groundwater and surface water were collected for two dry and wet seasons during the water year of 2011–2012.The study shows that most of the groundwater in the aquifer falls above the global meteoric water line,and all the samples fall below the Mediterranean meteoric water line,indicating that these samples are a mixture of two water types.The tritium content of these samples supports this conclusion.The overall conclusion of this study indicates that there are two sources of groundwater recharge in the studied area:the ephemeral streams(Teeb and Dewerge) and major precipitation sources.According to the tritium levels at or below one tritium unit(TU) obtained from the water,supply wells are highly confined or "not vulnerable".Overall,the 3H results imply that recent recharge has taken place during the last four to five decades.In the recharge area,the high tritium content in the southern part of the Teeb area suggests that the recharge originates from rapid infiltration of surface runoff.Therefore,the groundwater resources in the study area should be protected from contamination,because it will influence the aquifer in a relatively short period of time if any contamination enters the recharge areas of the aquifer.     

长江口水域春季鱼卵仔鱼分布及其与温度盐度的关系

利用2003年春季在长江口水域进行调查的数据,对该水域的鱼卵仔鱼的种类组成和数量分布、表层水温、盐度的分布特征以及它们之间的关系进行了研究。结果表明:共捕获鱼卵仔鱼13种,出现率为81.25%。鱼卵出现站位资源密度(CPUE)为1～760粒/网,平均每网采获鱼卵63.6粒;仔稚鱼出现站位CPUE为1～174尾/网,平均每网捕获仔稚鱼18.75尾。按照表层温度、盐度的变化,该水域可分为高温低盐、低温高盐、低温低盐三个区。鱼卵出现水域主要集中在高温低盐区;而仔稚鱼在长江口水域广有分布,但主要出现水域是高温低盐和低温低盐区控制的近岸水域,在远离海岸的低温高盐区仅有少量的分布。     

坛紫菜ISSR反应体系的建立与优化

为了利用ISSR分子标记技术对坛紫菜(Porphyra haitanensis)种质资源进行遗传分析及种质鉴定,获得清晰稳定、重复性好、多态性高的扩增结果,对影响ISSR-PCR扩增的多个因素,包括DNA模板含量、Mg2+浓度、TaqDNA聚合酶含量、引物浓度、dNTP浓度以及复性温度进行了全面比较和优化,建立了坛紫菜种质资源ISSR-PCR扩增的最佳反应体系:25μL的反应体系中含2.5μL 10×PCR缓冲液,5 ng模板DNA,2.5 mmol/L Mg2+,1.5 UTaqDNA聚合酶,200 nmol/L引物,200μmol/L dNTP。最佳PCR扩增程序:94℃预变性7 min;每个循环94℃变性1 min,48℃复性45 s,72℃延伸2 min,共进行35个循环;循环结束后72℃再延伸7 min。