斑马鱼体细胞核移植-头肾细胞和尾鳍细胞核移入成熟具核卵子发育能力的初步研究

将经直接消化的斑马鱼头肾细胞或经短暂培养的尾鳍细胞核移植到同种成熟具核卵子中 ,移核卵可发育到原肠晚期 ;将移核卵发育成的囊胚细胞核作供体进行第 2次核移植 ,受体卵可发育到尾芽期或肌肉感应期。流式细胞仪测定 DNA含量表明 ,受体卵发育形成的囊胚细胞 DNA含量与正常囊胚细胞 DNA含量相同 ,由此可知 ,移核卵中的雌原核没有参与移核胚胎的发育。     

IN VITRO STUDY ON CYTOTOXIC EFFECTS OF THE ORGANOPHOSPHOROUS PESTICIDE PROFENOFOS ON THE GILL CELL LINE,FG-9307, OF THE FLOUNDER （PARALICHIHYS OLIVACEUS）

The FG-9307 cell line derived from the gill of flounder Paralichthys olivaceus was used in the present study to determine the acute cytotoxic effects of the organophosphorous pesticide, profenofos. It was found that the cell growth rate was markedly reduced by profenofos at the concentrations of 2.5, 5 and 7.5 mg/L tested; and that the fine structures of the cells were also altered by profenofos, as evidenced by dilation of nuclear membranes and mitochondria cristae, and presence of enlarged lysoscrneswith engulfed organelles and numerous vacuoles in the cytoplama. Probably, mitochondria, the cell ener-gy-generating sites, are the most prcrninent sites of profenofos cytotoxity in the cells. This seems to be the first report of the use of marine fish cell line for evaluation of the acute in vitro cytotoxicity of organophos-phorus pesticide.     

IN VITRO STUDY ON CYTOTOXIC EFFECTS OF THE ORGANOPHO SPHOROUS PESTICIDE PROFENOFOS ON THE GILL CELL LINE, FG-9307, OF THE FLOUNDER (PARALICHTHYS OLIVACEUS)

ＩＮＴＲＯＤＵＣＴＩＯＮＰｏｌｌｕｔｉｏｎｏｆｍａｒｉｎｅｅｃｏｓｙｓｔｅｍｐｏｓｅｓａｓｅｒｉｏｕｓｔｈｒｅａｔｔｏｍａｒｉｎｅｏｒｇａｎｉｓｍｓａｎｄｕｌｔｉｍａｔｅｌｙｔｈｅｅｎｔｉｒｅｅｃｏｓｙｓｔｅｍ.Ｉｎｒｅｃｅｎｔｙｅａｒｓ,ｏｒｇａｎｏｐｈｏｓｐｈｏｒｏｕｓ(ＯＰ)ｐｅｓｔｉｃｉｄｅｓｈａｖｅｌａｒｇｅｌｙｒｅｐｌａｃｅｄｏｒｇａｎｏｃｈｌｏｒｉｎｅｐｅｓｔｉｃｉｄｅｓｂｅｃａｕｓｅｏｆｔｈｅｉｒｒａｐｉｄｂｒｅａｋｄｏｗｎｉｎｗａｔｅｒａｎｄｔｈｅｉｒｌｏｗｅｎｖｉｒｏｎｍｅｎｔａ…     

调节因子X蛋白家族的结构与功能

调节因子X(Regulatory Factor X, RFX)蛋白家族是一类包含高度保守的有翼螺旋型DNA结合域的转录因子,因特异性结合基因启动子区的X盒而得名。该蛋白家族广泛分布于动物和真菌中,参与纤毛和精子发生、免疫反应以及细胞周期调控等多种生物过程。RFX家族的一些成员还对肿瘤细胞的存活及增殖起到促进或抑制作用,有望成为治疗肿瘤的新靶点。本文将对RFX蛋白家族的发现、各成员的结构特征、表达模式和生物学功能等进行综述,以期待为后续研究提供参考。     

In vitro acute cytotoxicity of neonicotinoid insecticide imidacloprid to gill cell line of flounder Paralichthy olivaceus

In vitro acute cytotoxicity of neonicotinoid insecticide imidacloprid (IMI) to the gill cell line of flounder (FG) that collected in the gill of Paralichthys olivaceus, was examined by 3 widely used endpoint bioassays: NR (neutral red), MTT (3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) and TCP (total cell protein). The result shows that the IMI increased at concentrations ≥0.5 μg/ml. The IC50 value of NR, MTT, and TCP was 41.86, 38.46, and 39.08 μg/ml, respectively. The ultrastructural observation revealed that the mitochondria of the cells exposed to 60 μg/ml IMI for 48 h were severely damaged, swollen or disrupted, while their nuclei and rough endoplasmic reticulum (RER) remained normal. This would suggest that the mitochondria are probably the primary target of IMI.     

卵黄蛋白原研究及其进展

卵黄蛋白原是特异地存在于非哺乳类性成熟的卵生雌性动物血液中的一种蛋白 ,是几乎所有卵生动物卵黄蛋白的前体。Ｖｇ最初是Ｐａｎ等人［１５］１９６９年对雌性昆虫血液淋巴蛋白的特称 ,后来这一名称被广泛用于特指雌性动物血浆中卵黄蛋白原。Ｖｇ为正在发育的胚胎提供氨基酸、脂肪、碳水化合物、维生素、磷和硫等营养和功能性物质。随着研究的深入,人们发现 ,卵黄蛋白原不仅为胚胎发生时提供营养物质 ,它在生物体内还具有其他的生物学功能。１卵黄蛋白原的合成方式卵子发生过程中卵黄的形成对于胚胎发生时所需营养物质的提供具有至关重要…     

酚氧化酶研究概况Ⅰ——特性、功能、分布和在胚胎发育中的变化

酚氧化酶(phenoloxidase, PO)参与无脊椎动物的防御反应.在无脊椎动物中, PO一般以无活性的酶原形式--酚氧化酶原(prophenoloxidase, proPO)存在[1].     

硝化-膜生物反应器处理氨氮废水的研究

为充分考察膜生物反应器的硝化性能 ,采用活性污泥比消化速率 ,对膜生物反应器处理氨氮废水的硝化性能进行了研究。结果表明 :在容积负荷 1.0 96kgNH+ 4 -N/(m3 ·d)的条件下 ,硝化性能稳定 ,氨氮转化为硝酸盐氮的转化率可达 99% ;亚硝化菌和硝化菌在活性污泥中占优势 ,系统中出现了胞外多聚物和可溶性微生物产物的积累。     

Genes of the adaptive immune system are expressed early in zebrafish larval development following lipopolysaccharide stimulation

Information regarding immunocompetence of the adaptive immune system(AIS) in zebrafish Danio rerio remains limited.Here,we stimulated an immune response in fish embryos,larvae and adults using lipopolysaccharide(LPS) and measured the upregulation of a number of AIS-related genes(Rag2,AID,TCRAC,IgLC-1,mIg,sIg,IgZ and DAB) 3 and 18 h later.We found that all of the genes evaluated were strongly induced following LPS stimulation,with most of them responding at 8 d post fertilization.This confirms that a functional adaptive immune response is present in D.rerio larvae,and provides a window for further functional analyses.     

用ELISA检测中国对虾的白斑综合症病毒(WSSV)

随着对虾养殖业的发展,对虾疾病越来越受到人们的关注,尤其对虾病毒性疾病已成为各国学者研究的焦点之一。由于没有有效的抗病毒药物,早期预防就显得更为重要,而这依赖于快速、敏感的诊断方法。酶联免疫吸附试验(enzyme-linked immunosorbent assay,ELISA)是1972年由Engvall等在酶免疫试验(enzyme immunoassay,EIA)的基础上发展起来的。目前,在病毒等疾病有关的抗原抗体系统的定性或定量测定领域内已被广泛应用。本文通过病毒纯化,免疫新西兰兔,获得多克隆抗血清,并对抗体进行纯化、酶标记,建立了双抗体夹心直接ELISA的检测方法。