LOCALIZATION OF A PUTATIVE EPIBOLY-DETERMINING FACTOR IN OOCYTES OF THE GOLDFISH (CARASSIUS AURATUS)

It is unknown whether cytoplasmic determinants in goldfish eggs are asymmetrically localized beforematuration. In this study fully grown goldfish oocytes with infact germinal vesicles (GVs) were ligated withbaby hair loops along desired planes into two parts, mature in vitro, and inseminated. Embryos developedfrom the animal halves with GV of oocytes ligated along the equator un unable to undergoepiboly, while those developed from the GV-containing parts of oocytes ligated along the animal-vegetal axiswere able to. Also, embryos developed from the animal halves obtained from fertilized eggs could undergo epiboly. From there results, it was postulated that the goldfish's premature oocyte possessed a putativefactor in the vegetal hemisphere which was responsible for epiboly of the embryonic cells.     

毛蚶血清凝集素的凝集活性

以毛蚶为实验材料,对其血清中的凝集素进行研究.毛蚶血清凝集素对人的A,B,O血型红细胞、鸡和小鼠的红细胞均有凝集作用,凝集效价分别为256,256,8,16,64,同时它还能对白色念珠菌、啤酒酵母、溶藻胶弧菌等微生物具有凝集作用.毛蚶血清凝集素具有一定的pH值稳定性和热稳定性.在选用的12种单糖和寡糖中,L-鼠李糖、L-树胶醛糖、麦芽糖、D-半乳糖、蔗糖对毛蚶血清的凝集活性有明显的抑制作用.用弧菌对毛蚶进行免疫刺激时,其血清对鸡红细胞的凝集效价明显增加.     

cDNA代表性差异分析和抑制性减法杂交技术的比较

本文就cDNA代表性差异分析和抑制性减法杂交这两种技术的主要原理、基本操作过程和优缺点进行了介绍和比较，并展望了这两种技术在海洋生物技术领域的应用前景。     

为科学事业奋斗终生——深切悼念吴尚懃教授

1988年3月,吴尚懃教授在野外工作时,不幸车祸遇难,过早地离开了我们,离开了她所热爱的细胞分化的研究工作,至今已一年多。每当我们走进她原先的实验室时,总有一种说不出的情感,不由自主的使我们想起曾经为科研工作奋斗一生的老科学家——吴尚懃教授。     

斑马鱼体细胞核移植-头肾细胞和尾鳍细胞核移入成熟具核卵子发育能力的初步研究

将经直接消化的斑马鱼头肾细胞或经短暂培养的尾鳍细胞核移植到同种成熟具核卵子中 ,移核卵可发育到原肠晚期 ;将移核卵发育成的囊胚细胞核作供体进行第 2次核移植 ,受体卵可发育到尾芽期或肌肉感应期。流式细胞仪测定 DNA含量表明 ,受体卵发育形成的囊胚细胞 DNA含量与正常囊胚细胞 DNA含量相同 ,由此可知 ,移核卵中的雌原核没有参与移核胚胎的发育。     

IN VITRO STUDY ON CYTOTOXIC EFFECTS OF THE ORGANOPHOSPHOROUS PESTICIDE PROFENOFOS ON THE GILL CELL LINE,FG-9307, OF THE FLOUNDER （PARALICHIHYS OLIVACEUS）

The FG-9307 cell line derived from the gill of flounder Paralichthys olivaceus was used in the present study to determine the acute cytotoxic effects of the organophosphorous pesticide, profenofos. It was found that the cell growth rate was markedly reduced by profenofos at the concentrations of 2.5, 5 and 7.5 mg/L tested; and that the fine structures of the cells were also altered by profenofos, as evidenced by dilation of nuclear membranes and mitochondria cristae, and presence of enlarged lysoscrneswith engulfed organelles and numerous vacuoles in the cytoplama. Probably, mitochondria, the cell ener-gy-generating sites, are the most prcrninent sites of profenofos cytotoxity in the cells. This seems to be the first report of the use of marine fish cell line for evaluation of the acute in vitro cytotoxicity of organophos-phorus pesticide.     

IN VITRO STUDY ON CYTOTOXIC EFFECTS OF THE ORGANOPHO SPHOROUS PESTICIDE PROFENOFOS ON THE GILL CELL LINE, FG-9307, OF THE FLOUNDER (PARALICHTHYS OLIVACEUS)

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In vivo effects of 17-β-estradiol on plasma immunoglobulin levels and leukocyte density in zebrafish Danio rerio

Estradiol, or 17-β-estradiol (E2), the most potent naturally occurring estrogen, is involved in the hormone-immune system interaction in both mammals and fish. However, in vivo studies are largely limited, and little is known about whether E2 exerts similar effects on both female and male zebrafish (Danio rerio). Here, we show exposure of both sexes of D. rerio to 20 nmol/L E2 resulted in a significant increase in Vg1 expression, but caused little damage to the hepatocytes, suggesting that this is the optimum E2 concentration. Also, exposure to 20 nmol/L E2 for 20 days caused a marked increase in plasma IgM levels, but had little influence on the peripheral leukocyte density, providing the first evidence of a hormone-immune system interaction in this species.     

激素诱导欧氏六线鱼性腺发育的初步研究

随着鱼类成为人工养殖对象,人们越来越希望养殖鱼类能在人为控制下成熟和产卵,从而打破自然环境下的繁殖周期,为养殖生产提供更多的、适合生产需要的苗种,本研究采用LRH-a(促性腺释放激素类似物),17β-雌二醇及以生理盐水作为对照组,使用腹腔注射的方法,对欧氏六线鱼的性腺发育进行诱导实验,旨在探讨激素对鱼类性腺发育的影响。1　材料和方法1996年9～10月间,取网箱暂养的欧氏六线鱼39尾,随机取7尾以观察实验前六线鱼的性腺发育状况,逐尾称体重,解剖取出生殖腺和内脏,称量生殖腺重和净重,计算性腺指数,并求出其平均值,♀:0.64±0.10,♂:0.…     

PRODUCTION OF FERTILE EGGS AND SPERMS IN LABORATORY-REARED AMPHIOXUS BRAN- CHIOSTOMA BELCHERI TSINGTAUNESE

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