Expression of putative zinc-finger protein lcn61 gene in lymphocystis disease virus China （LCDV-cn） genome

An open reading frame (lcn61) of lymphocystis disease virus China (LCDV-cn), probably responsible for encoding putative zinc-finger proteins was amplified and inserted into pET24a (+) vector. Then it expressed in E. coli BL21 (DE3), and His-tag fusion protein of high yield was obtained. It was found that the fusion protein existed in E. coli mainly as inclusion bodies. The bioinformatics analysis indicates that LCN61 is C2H2 type zinc-finger protein containing four C2H2 zinc-finger motifs. This work provides a theory for functional research of lcn61 gene. Supported by High Technology Research and Development Program of China (863 Program, No. 2006AA100309)     

Chemical signature of intermediate water masses along western Portuguese margin

Although the circulation of intermediate water masses in the eastern North Atlantic remains poorly defined, the presence of fresher intermediate waters, the Sub-Artic (SAIW) and the Antarctic Intermediate Water (AAIW), as well the saline intermediate Mediterranean Water (MW), has been tracked using biogeochemical properties. Here we assess the hydrographic and chemical structures of intermediate waters along the western Portuguese margin by examining the vertical distributions and property-property plots of chemical tracers (oxygen and nutrients). AAIW was traced by low oxygen and high nutrients, while SAIW was recognized by low nutrients. The Mediterranean Water (MW) undercurrent is shown to spread towards the eastern flank of Gorringe bank. Concurrently, the fresher waters gained salt by direct incorporation of MW, while this water was enriched in nutrients on its way northward and westward owing, to a great extent, to the entrainment of an AAIW branch. The distributions of nutrients and apparent oxygen utilization are discussed in terms of regional ocean circulation. Our analysis suggests a circulation pattern of the various intermediate waters along the western Portuguese margin: MW extends all over the area, but its presence is more pronounced around cape St. Vincent; SAIW apparently moves southward, reaching the Gorringe bank region, and AAIW flows northward along the coast and around the bank.     

中国及邻区现代构造应力场的数值模拟

我国及邻区的现代构造应力场作为一平面应力问题用有限单元法进行了计算。将本区地壳看成是一不均匀的弹性板,根据各地区杨氏模量E、泊松比v和地壳厚度T的不同,组成12种材料区。全区被分成288个三角形单元。考虑了五种应力和位移边界条件,这些边界条件分别反映了印度洋板块、太平洋板块及菲律宾海板块对我国及邻区施加应力的相对大小。将计算得到的最大剪应力值与强震分布进行对比,将最大主压应力方向与震源机制解的最大主压应力方向进行对比,选择一种和实际符合得最好的边界条件作为可取的模式。结果表明:来自印度洋板块的作用力最大,大约是来自太平洋板块和菲律宾海板块作用力的两倍。     

Models of travel demand with endogenous preference change and heterogeneous agents

In the literatures of regional science, urban economics, and urban development planning, a working assumption is that individuals respond to incentives and regulations, given their preferences. Models for planning and policy analyses are used to consider what might occur if the incentives or regulations were different. In these models, however, preferences are usually assumed to be given and stable, and agents are usually assumed to be homogeneous. This paper focuses on the implications of making preferences in models of policy implementation endogenously determined and time varying heterogeneous agents. We consider first the recent literature on intertemporal choice and preference change, which cuts across many disciplines, and more briefly the literature on norm-regarding behavior. We then elaborate a simple model of transportation demand—from a static to a dynamic orientation, from fixed and exogenously given preferences of strictly self-regarding agents to endogenously determined and policy-induced preferences of heterogeneous agents—and illustrate its characteristics with simple numerical examples.     

DEVELOPMENT OF DETENTION VOLUME MODEL FOR DETENTION PONDS WITH LONG-DURATION RAINFALL

The detention pond is one of the crucial items in detention facilities. It may effectively alleviate the occurrence of peak discharge, control the center of flood flow, and reduce the amount of soil loss. The objective of this study is analyzing the detention volume change of a detention pond with long-duration rainfall under the known isosceles trapezoidal inflow hydrograph model. The volume change of detention, which is under the influences of a givenisosceles trapezoidal inflow hydrograph and the extent of peak attenuation, is investigated by using the non-dimensional detention theory and the related mathematical analyses. The minimum detention volume of a detention pond can therefore be calculated based on the estimated of volume change of detention. The proposed detention volume estimation model can be used for the design of detention of facilities during the hillside development.     

Application of saddlepoint approximation in reliability analysis of dynamic systems

The application of the saddlepoint approximation to reliability analysis of dynamic systems is investigated. The failure event in reliability problems is formulated as the exceedance of a single performance variable over a prescribed threshold level. The saddlepoint approximation technique provides a choice to estimate the cumulative distribution function (CDF) of the performance variable. The failure probability is obtained as the value of the complement CDF at a specif ied threshold. The method requires computing the saddlepoint from a simple algebraic equation that depends on the cumulant generating function (CGF) of the performance variable. A method for calculating the saddlepoint using random samples of the performance variable is presented. The applicable region of the saddlepoint approximation is discussed in detail. A 10-story shear building model with white noise excitation illustrates the accuracy and effi ciency of the proposed methodology.     

Lateral continuity of basement seismic reflections in 15 Ma ultrafast-spreading crust at ODP Site 1256

The Ocean Drilling Program (ODP) initiated drilling at Site 1256D in the Guatemala Basin, about 1,000 km off the East Pacific Rise to penetrate plutonic rocks, anticipated to be relatively shallow in this region, formed at an ultra-fast spreading rate. IODP Expedition E312 successfully drilled into gabbros at ~1,150 m in basement. Multi-channel seismic traces show weak laterally coherent sub-basement reflections at borehole depths. Synthetic reflectivity seismograms were computed using a Ricker wavelet and impedance profiles from borehole sonic logs. These seismograms show significant sub-basement amplitude peaks. A zero-offset vertical seismic profile, shot on E312, was processed to investigate the authenticity of these reflections and their relationship to borehole geology. A dual scheme of the median filtering and F–K dip filtering was used. Tests with synthetic seismograms indicate the approach is effective at reasonable SNR levels. Downgoing energy is clearly identified but negligible upgoing energy is visible over random noise. These results indicate that lava flows and igneous contacts in upper ocean crust have significant topography on lateral scales less than the Fresnel Zone (~300 m) due to igneous and tectonic processes.     

Comparison of ecophysiological characteristics between introduced and indigenous mangrove species in China

Due to its rapid growth, the introduced mangrove species Sonneratia apetala from Bangladesh has been widely used in mangrove restoration in southeastern China since 1985. As an indigenous mangrove species in Hainan, China, Sonneratia caseolaris was also planted in Guangdong Province for afforestation purposes. Both species have developed well in their new habitats, but their ecophysiological differences with the native mangrove species have not been studied. In this study, leaf gas exchange, water and nitrogen use efficiencies of two Sonneratia species were compared with those of selected native mangrove species (Avicennia marina, Aegiceras corniculatum, Kandelia candel, and Excoecaria agallocha) in Hainan and Shenzhen. The introduced S. apetala maintained lower carbon assimilation rate (A) and photosynthetic nitrogen use efficiency (PNUE) than the indigenous S. caseolaris. In Shenzhen, the two introduced Sonneratia had comparable photosynthetic rates and water use efficiency (WUE) with the native mangrove species, except that PNUE in S. caseolaris was significantly higher than in the native mangrove species. The two Sonneratia species showed significant overlap in PNUE and long-term WUE. Photosynthetic parameters derived from leaf photosynthetic light–response curves and A–C_i curves also suggested lower carbon assimilation capacities for the introduced Sonneratia than for the native mangrove species in both study sites. The lower light compensation point (LCP) of two introduced Sonneratia in both study sites also indicated a better adaptation to a low light regime than the native mangrove species. The results of photosynthetic capacities indicated that the introduced mangrove species have little competitive advantage over local native mangrove species in their respective new habitats.     

An Analytical Model For Threshold Crossing Rates Of Concentration Fluctuations In Dispersing Plumes

A simple analytical model is developed for the meanupcrossing rate of plume concentration fluctuations assuming that thisprocess can be well approximated by a lognormal process. The resultingexpression requires only the specification of the in-plume fluctuationintensity and in-plume Taylor micro-time scale and, hence, does notexplicitly involve the joint probability density function of theconcentration and its derivative. The analytical model provides agood fit to some field measurements of the mean upcrossing rate ina dispersing plume.     

Study on uncertainty of inversion and resolution limit in the presence of noise

This study analyzed the uncertainty of inversion and the resolution limit in the presence of noise by means of statistical experiments. The exhaustive method is adopted to obtain the global optimal solution in each experiment. We found that even with small level of noise, solutions fluctuate in a large range for the thin bed. The distribution of solutions in the presence of noise is closely related to the spread of the cost function in the absence of noise. As a result, the area of a certain neighborhood around the true solution on the spread of the cost function in the absence of noise is used to evaluate the uncertainty of inversion and the resolution limit in the presence of noise. In the case that the SNR (signal-to-noise ratio) is 5 in this study, solutions focus around the true solution with a very small uncertainty only when the bed thickness is greater than the reciprocal of the double predominant frequency of the convoluting wavelet.