海藻硫酸多糖911对小鼠脾淋巴细胞增殖反应及对细胞产生IL—2作用的影响

本文以~3H—TdR掺入法观察911对小鼠脾淋巴细胞增殖反应的影响并用CTLL细胞检测了其对IL—2的作用。体外实验结果表明,911对小鼠脾淋巴细胞增殖反应有明显的增强作用,以0.5μg/ml的浓度效果最为明显,相对增殖指数RPI可达200％;体内实验则以5mg/kg体重ip,连续7d效果最好,相对增殖指数RPI可达176％;911用药组小鼠IL—2的产生量均高于对照组,以5mg/kg和10mg/kg效果最好。以上实验证明,这一多糖是一种有希望的新免疫调节药物。     

中国对虾体内20-羟基蜕皮酮含量与生长和性腺发育的关系

应用放射免疫分析法对完成变态后的中国对虾(Penaeus orientalis)体内20-羟基蜕皮酮(20-HE)含量进行了周年测定。5～6月,全虾抽提液中20-HE的质量分数以体长为0.8cm的仔虾为最高(47.96±3.94)×10~(-9)(m/m)。血淋巴中20-HE的质量浓度在6～8月(平均为22.66ng/mL)和11月(25.93±3.44ng/mL)较高,翌年3月最低,为8.29±1.40ng/mL。呈现出20-HE含量与蜕皮生长和促进卵母细胞早期发育及精子发生有关。     

Overtopping Discharge of Wind-Waves on Simple Slope Dikes

An empirical formula for estimating the overtopping discharge of wind-waves on a smooth-impermeable-simple slope dyke is derived through model tests in this paper, it can be adopted by related design departments in the determination of the crest elevation of the dyke.     

渤海湾海河口区耗氧有机物的分布特征

渤海湾海河口区是北京、天津二大城市工业及生活污水排放入海处，是一比较典型的海域。1972年以来曾对渤海湾进行过综合性的环境污染调查和研究，但由于受调查范围和项目的限制，对河口区特别是大沽口、北塘口区有机污染物的迁移转化研究甚少，尚不足以就有机污染对河口生态环境的潜在影响作出科学判断。目前，有机污染已成为海河口区特别是其附近大城市的主要污染问题之一。本文以化学耗氧量(COD)、生物耗氧量(BOD5)、溶解氧(DO)等项目作为耗氧有机物污染程度评价的主要指标(还有氮、磷、本集另有专文讨论)，对海河口区有机污染物的迁移、转化及生态效应进行了调查研究，为有机污染评价提供数据并作初步探讨。     

条斑紫菜(Porphyra Yezoensis Ueda)和孔石莼(Ulve Pertusa Kjellm)对酸耐受力的研究——Ⅰ.pH对条斑紫菜和孔石莼呼吸强度和致死酸度试验

不同pH海水和不同时间处理紫菜、石莼对呼吸强度和致死酸度试验结果表明,紫菜比石莼耐酸力强。在海水温度为18℃条件下,分别用pH_2、pH_3、pH_4、pH_5、pH_6、pH_7、pH_8处理,处理时间20分钟,紫菜除pH_2处理对呼吸强度有抑制作用和培养5天后有20％死亡外,pH_3、pH_4、pH_5、pH_6、pH_7、pH_8各组处理对呼吸强度都有促进作用,培养5天后没有死亡现象,而石莼经pH_2、pH_3处理,对呼吸强度都有抑制作用,培养5天后,pH_2处理组全部死亡,pH_3处理有50％死亡。pH_3和pH_4在海水温度为18℃条件下,处理时间为40分钟时,对紫菜呼吸强度有促进作用,培养5天报,pH_3处理组仅有20％死亡。而pH_3对石莼呼吸强度有抑制作用,培养5天后,全部灭亡。在生产上是否可以考虑用pH_3的海水处理紫菜网帘以达到消除石莼(杂藻)的目的,将是一项具有生产意义的待试验课题。     

Practical implementation of Hilbert-Huang Transform algorithm

Hilbert-Huang Transform (HHT) is a newly developed powerful method for nonlinear and non-stationary time series analysis. The empirical mode decomposition is the key part of HHT, while its algorithm was protected by NASA as a US patent, which limits the wide application among the scientific community. Two approaches, mirror periodic and extrema extending methods, have been developed for handling the end effects of empirical mode decomposition. The implementation of the HHT is realized in detail to widen the application. The detailed comparison of the results from two methods with that from Huang et al. (1998, 1999), and the comparison between two methods are presented. Generally, both methods reproduce faithful results as those of Huang et al. For mirror periodic method (MPM), the data are extended once forever. Ideally, it is a way for handling the end effects of the HHT, especially for the signal that has symmetric waveform. The extrema extending method (EEM) behaves as good as MPM, and it is better t     

Molecular identification of bloom-forming species Phaeocystis globosa (Pryninesiophyta) and its dispersal based on rDNA ITS sequence analysis

The colony-forming Phaeocystis species are causative agents of dense bloom occurrences in coastal waters worldwide. It is difficult to separate them because of the different morphologies associated with their colonial stages. In this study we applied molecular approaches to analyze the genetic variation of Phaeocystis globosa and Phaeocystis pouchetii from several geographic regions, and to assist in tracing the dispersal of bloom-forming Phaeocystis species in coastal waters of China. The sequences of the internal transcribed spacers (ITS1 and ITS2) of rDNA and the 5.8S ribosomal RNA gene of Phaeocystis strains were determined. Sequence comparison shows that P.globosa was the most divergent to P. pouchetii, exhibiting sequence divergence higher than 0.08. However, lower genetic divergences existed between strains of P.globosa. The sequence comparison of the Phaeocystis rDNA ITS clearly shows that the species isolated from the southeast coast of China is identified as P.globosa rather than P. cf. pouchetii or other species. Furthermore, the significance of rDNA variation in distinct global populations of P.globosa suggested it might have had sufficient time to accumulate detectable mutations at the rDNA locus, supporting the hypothesis of ancient dispersal of P.globosa to many areas, meaning that P.globosa blooms in the coastal waters of China are endemic rather than a newly introduced species or a foreign source. Finally, based on the high divergent region of rDNA ITS, a pair of species-specific primers for P.globosa were designed, they could be useful to detect the presence of this species in mixed plankton assemblages or flagellate stages that are recognized with diffculties by means of conventional microscopy.     

Effects of chemical ecological adjustment and control experiment on phytoplankton community in the Aoshan Bay

There is a low nutrient level in the Aoshan Bay. In June 1999, the chemical adjustmentand control experiment was made in the Aoshan Bay. Following tracts investigation was carried out before the experiment and on the 1st, 2nd, 4th, 5th, 6th and 45th day/after the experiment. While the variance of amount of phytoplankton, the replacement of superior species and the species composition of phytoplankton were researched. The results show that the amount of phytoplankton in the Aoshan Bay rises gradually after the experiment. Ceratium macroceros Cleve of pyrophyta was the dominant species before the experiment, its dominant index was 37.7％. Six days after the experiment, its dominant index dropped to 17.6％ . Meanwhile the dominant index of Asterionella japanics Cleve rose from 7.1％ to 39.2％ , it became the first dominant species. Forty-five days after the experiment, the amount of phytoplankton in the Aoshan Bay was 5.15 to 137.32 times more than that in 1997.     

几丁糖酯原料药稳定性研究

该文建立高效凝胶渗透色谱法 (HPGPC) ,明胶比浊法 ,氧瓶燃烧法三种分析方法考察海洋硫酸多糖药物几丁糖酯的稳定性 ,采用这三种方法分别考察几丁糖酯原料药在高温、高湿、强光照射及加速条件下分子量及分布宽度、样品中硫酸根含量及样品含量的变化情况。结果表明 :三种方法均适用于几丁糖酯的稳定性考察 ,几丁糖酯在上述影响因素条件下稳定性良好     

利用卫星遥感技术监测赤潮的研究

赤潮灾害的频繁发生，给海洋生态环境和海洋渔业经济造成了严重危害。卫星遥感技术的不断完善，为快速、有效地动态监测赤潮灾害提供了可能，通过卫星图片的校正、合成、分析、解释、判断分析，可以迅速连续地掌握赤潮的发生状况，对于研究防控和治理赤潮灾害有着十分重要的意义，本文就如何利用卫星遥感技术监测赤潮进行了探讨。