Polyclonal antibodies to light-harvesting CHL-protein of PSII (LHC II) in marine green algaeBryopsis corticulans

Polyc·lonal antibodies raised against LHC II isolated from SDS-solubilizedBryopsis corticulans thylakiod membranes by SDS-PAGE, were characterised by double immunodiffusion, Rocket immunoelectrophoresis and antigen-antibody crossed immunoelectro-phoresis assays showed the antibodies had strong cross-reaction with allB. corticulans LHC II components (even with those which were incubated in boiling water) and showed immunological cross-reactivity with LHC II polypeptides of spinach and the marine green algaCodium fragile. The results suggested that LHC II of different species had similar antigenic determinants and also conservation of amino acid sequences of the polypeptides during evolution, and that the antibodies could cross react with apoproteins of D₂ proteins (which contain P₆₈₀) fromB. corticulans, spinach andC. fragile, but not with apoproteins of P₇₀₀ Chl-proteins. Our results indicated some similarities in primary structure between LHC II of different species, and between LHC II and D₂ proteins of marine green algae and spinach. Our finding that D₂ and P₇₀₀ Chl-proteins are not immunologically related suggested that P₇₀₀ Chl-proteins and D₂ proteins pass through independent evolutionary pathways. Contribution No 1717 from the Institute of Oceanology, Academia Sinica, This subject was supported by the National Science Foundation of China.     

光照强度对海洋微藻脂肪含量及脂肪酸组成影响的研究

近年来，海洋微藻脂肪酸组成的研究及其应用越来越受到国内外科学家的重视。高度不饱和脂肪酸(PUFA)，特别是长链的n-3 PUFA，如二十碳五烯酸(EPA)和二十二碳六烯酸(DHA)对海洋动物和人类都具有营养学和医学上的价值。在海洋微藻的培养过程中，光照强度是海洋微藻生长的条件之一，光线的明与暗，光度的强与弱，不仅对微藻的生长速率、产量有影响，而且对其脂肪含量和脂肪酸的组成也有影响(Teshima et al.，1983；Thompson et al.，1990； Renaud et al., 1991)。 作者在以往研究的基础上，选择了3种有代表性的海洋微藻：(1)小球藻Chlorella sp-2(李荷芳等，1999)，此藻脂肪酸中EPA含量高、且不含DHA；(2)球等鞭金藻(Isochrysis galbana)，该藻DHA含量较高，但几乎不含EPA；(3)前沟藻(Amphidinium sp.)的EPA、DHA含量均高。将以上3种微藻作为原料，在不同的光照强度下进行培养，测定并分析藻体中的脂肪含量和脂肪酸组成的变化，从而了解光强对海洋微藻脂肪含量及其脂肪酸组成的影响。     

Centrifuge modelling of ground-borne vibrations induced by railway traffic in underground tunnels

Increased attention has been given to ground-borne vibrations induced by railway vehicles and to the effects of these vibrations as they propagate through the ground into nearby buildings.Various studies,mainly based on numerical methods as well as physical modelling,have been carried out to investigate this problem.To study the dynamic response of tunnels and the surrounding soil due to train-induced vibration loads,a centrifuge te st was conducted with a small-scale model in 1 g and 50 g stres...     

四川省芦山—宝兴地区花岗石石材产出地质特征及其品质

本文扼要阐明四川省芦山－宝兴地区花岗石石材产出地质特征的基础上，重点讨论花岗石石材的品种、规模和质量。     

Neptune Trojans: a Revisit and a New Membertwo

Up to now, 17 Neptune Trojan asteroids have been detected with their orbits being well determined by continuous observations. This paper analyzes systematically their orbital dynamics. Our results show that except for two temporary members with relatively short lifespans on Trojan orbits, the vast majority of Neptune Trojans located within their orbital uncertainties may survive in the solar system age. The escaping probability of Neptune Trojans, through slow diffusion in the orbital element space in 4.5 billion years, is estimated to be ～50%. The asteroid 2012 UW177 classified as a Centaur asteroid by the IAU Minor Planet Center currently is in fact a Neptune Trojan. Numerical simulations indicate that it is librating on the tadpole-shaped orbit around the Neptune's L₄ point. It was captured into the current orbit approximately 0.23 million years ago, and will stay there for at least another 1.3 million years in the future. Its high inclination of i ≈ 54^° not only makes it the most inclined Neptune Trojan, but also makes it exhibit the complicated and interesting co-orbital transitions between the leading and trailing Trojans via the quasi-satellite orbit phase.     

中国对虾(Penaeus chinensis)四倍体诱导研究

利用光控和行为学原理,控制中国对虾在适当时候产卵,在一定的时刻以温度休克和细胞松弛素的方法诱导受精卵发育成为四倍体。作者发展了对虾染色体制备技术,以中肠和触角腺、精巢为材料,从后期幼体直到8～9cm左右次成虾均获得较好的分裂中相。染色体倍性检测结果表明,最好的四倍体诱导成功率达66.7％。共获得10cm左右的实验对虾几千尾。初步观察表明,四倍体中国对虾具有一定的生长优势。     

鄂尔多斯盆地直罗油田长8储层成岩作用研究

通过岩芯及薄片的观察,结合扫描电镜、X衍射和阴极发光等资料分析,对直罗油田长8储层成岩作用及其对储层物性的影响做了研究。结果表明该研究区长8储层砂岩类型以中-细粒长石砂岩为主,碎屑颗粒以次棱角状为主,颗粒分选性好,胶结类型以孔隙-薄膜式胶结为主。研究区主要经历的成岩作用类型有压实作用、胶结作用、溶蚀作用等。整体处于晚成岩A期一晚成岩B期。压实作用、胶结作用使储集空间变小,对储层的破坏性较大,而溶蚀作用使储层物性得到一定的改善。     

A Study of the Peak Frequency of the Geosynchronous Spectrum in a Nonuniform Source

This paper investigates in detail the peak frequency of gyrosynchrotron radiation spectrum with self and gyroresonance absorption for a model of nonuniform magnetic field. It is found that the peak frequency shifts from lower frequency to higher frequency with increases in the low-energy cutoff, number density, input depth of energetic electrons, magnetic field strength and viewing angle. When the number density and temperature of thermal electrons increase, the peak frequency also shifts to a slightly higher frequency. However, the peak frequency is independent of the energy spectral index, high-energy cutoff of energetic electrons and the height of the radio source’s upper boundary. It is also found for the first time that there is a good linear correlation between the logarithms of the peak frequency and the low-energy cutoff, number density, input depth of energetic electrons, magnetic field strength, and viewing angle, respectively. Their correlation coefficients are higher than 0.95 and the standard errors are less than 0.06.     

污染分布下的Lp估计抗差测度

观测数据中含有粗差或异常值扰动的误差分布可视为污染分布。根据M估计理论,导出了Lp估计的影响函数,分析了Lp估计的抗差能力,并用效率来测度估计的优劣、综合抗差能力和效率,指出观测数据中含有粗差的扰动,应用p为[1．2．1．5]的Lp估计来进行处理。     

Assessment of the Excavation-Damaged Zone in a Tall Rock Slope Using Acoustic Testing Method

During slope excavation, high stresses can become concentrated in the rock mass because of stress redistribution. Failure of the rock mass creates an excavation-damaged zone (EDZ) in the slope. The damage reduces the acoustic wave velocity in the rock mass. Results of field tests measuring acoustic wave velocity at the Jinping I Hydropower Station are used here to study the EDZ in a tall rock slope. Two acoustic testing methods were adopted in the field tests: single-hole acoustic testing (SAT) and cross-hole acoustic testing (CAT). The acoustic wave velocity was lower in the EDZ, and the depth of the EDZ increased with decreasing slope elevation. Statistical analysis shows that the acoustic wave velocity obtained by the SAT method is larger than that obtained by the CAT method, and the relative difference between the SAT- and CAT-derived velocities is lower for a high quality rock mass than for a low quality rock mass. The integrity ratio and severity of damage can also be determined by acoustic wave velocity test results, revealing that the integrity ratio and elastic modulus of a rock mass are reduced in the EDZ.