淄博市区大气颗粒物重金属元素分布特征及其来源分析

为科学认识淄博市区大气颗粒物污染状况,探讨颗粒物的污染水平和元素在颗粒物中的分布与分配特征,对颗粒物中元素的来源进行分析。于2015年冬季和夏季期间分别布设45个和16个采样点采集悬浮颗粒物（TSP）、PM₁₀及PM_2.5样品,分析了颗粒物和18种元素（或化合物）的质量浓度。结果表明：1）大气颗粒物中冬季TSP、PM₁₀及PM_2.5的质量浓度均值高于夏季;PM₁₀和PM_2.5质量浓度均超过国家标准,其中冬季PM_2.5质量浓度是标准限值的1.96倍。2）不同粒径的颗粒物中元素质量分数差别显著,As、Cd、Cr、Cu、Ni、Pb、Zn等多数重金属元素主要富集在PM_2.5中,且PM_2.5约占PM₁₀的65%（冬季）和73%（夏季）。3）颗粒物PM_2.5中Al₂O₃、CaO、Fe₂O₃、K₂O、MgO等氧化物主要受土壤扬尘控制;As、Cd、Hg、Pb、Se、Zn等元素主要源于人为污染;Se的富集因子最高,反映了PM_2.5污染主要来源于燃煤;Co、Cu、Ni、Na₂O受土壤扬尘和人类活动的共同作用,而Cr具有混合污染的特点。     

全极化探地雷达地下管道分类识别技术

常规探地雷达大多数是单极化雷达,单极化雷达只能获得单极化数据,对复杂环境中管道准确快速地识别比较困难。为了解决此问题,本文采用了全极化探地雷达识别管道的方法,提取了单一管道目标、多个管道目标中任何一个管道目标和受其他目标影响的管道目标的极化属性。结果表明,全极化探地雷达技术对处于极化属性受到影响环境下的管道目标均能较好地识别。因此,全极化探地雷达能够获得更加全面的目标体极化信息,有效地解决了复杂环境中管道准确快速识别比较困难的问题。     

缢蛏天然抗性相关巨噬蛋白2(Sc-Nramp2)基因克隆及其在副溶血弧菌刺激下的表达分析

克隆得到缢蛏天然抗性相关巨噬蛋白2（Sc-Nramp2）基因的cDNA全长序列3 681 bp,该基因的开放阅读框有1 776 bp,编码591个氨基酸,预测分子量为65.86 kDa;其结构具有Slc11蛋白家族的典型特征,包括有10个典型的跨膜结构和2个糖基化位点。Sc-Nramp2基因3'-UTR有2个类似于脊椎动物Nramp2中铁反应控制蛋白结合位点;同源性分析表明,Sc-Nramp2和太平洋牡蛎Nramp2-like的同源性最高为71.6%。实时荧光定量PCR结果表明,Sc-Nramp2基因在闭壳肌、外套膜、肝胰腺、斧足、水管和鳃6个组织中均有表达,其中肝胰腺中的表达量最高,其次是鳃,与其他组织均有极显著性差异（P<0.01）;注射副溶血弧菌后,肝胰腺中Sc-Nramp2基因的表达量较对照组显著上调（P<0.01）,且表达量呈现先上升后下降的趋势,在12 h时达到最大表达量,推测Sc-Nramp2基因参与了缢蛏非特异性免疫应答反应。     

江苏中部淤泥质海岸岸线变化遥感监测研究

海岸线监测是了解海岸冲淤变化的基础。针对淤泥质海岸潮间带坡度平缓的特点,考虑到潮汐对遥感海岸线监测的影响,基于多潮位站插值校正的水边线离散点潮位赋值及坡度计算对水边线方法进行了改进,并结合潮间带实测坡度资料校正,推算遥感海岸线。选择江苏中部冲淤变化频繁、自然岸线保有率较高的扁担河口至川东港岸段开展海岸线变迁遥感监测研究。结果表明,研究区坡度主要在0.001~0.002之间,潮间带宽度由北向南越来越宽。北部扁担河口至射阳河口岸段处于冲刷环境中,大量以养殖塘围堤为主的人工岸线不断被侵蚀后退;射阳河口至四卯酉河口岸段以海岸线在自然状态下的动态变化为主,2010-2015年平均冲淤速率小于10 m/a,变化幅度较小;南部四卯酉河口至川东港岸段,自然岸线淤长明显,同时人工围垦导致岸线不断向海推进。根据监测结果,认为新洋港至斗龙港岸段应为研究区由北部侵蚀转向南部淤长的过渡带。     

文蛤亮氨酸氨肽酶LAP3的基因克隆及其在幼体不同发育时期、成体不同组织的表达分析

为探索贝类LAP3基因的结构和功能特征以及在生长发育过程中的作用,利用cDNA末端快速扩增（RACE）法获得文蛤LAP3（Mm-LAP3）基因的cDNA全长序列,并对其生物信息学、组织及发育时期表达特征进行了分析。结果显示,Mm-LAP3基因cDNA全长2 037 bp,ORF区1 254 bp,编码417个氨基酸;Mm-LAP3蛋白由Peptidase_M17超家族序列的N-端结构域和Peptidase_M17催化结构域组成。荧光实时定量PCR结果表明,Mm-LAP3基因在文蛤成体6个组织和幼体10个发育时期中均有表达,其中在斧足中的表达量显著高于其他组织（P<0.05）,这与斧足蛋白含量丰富、代谢旺盛相关;在幼体10个发育时期中的表达量呈现逐渐升高的趋势,在D形幼虫时期达到最高,随后又有所降低,推测Mm-LAP3基因在早期发育时期参与了某些组织器官的形成。     

The influence of increased iron concentration on survival and growth of seedlings and young plants of eelgrass Zostera marina

Iron (Fe) is an essential nutrient for plants but toxic at high concentrations. We subjected seedlings and young plants of eelgrass Zostera marina to different seawater iron concentrations (500, 600, 700, 800, 1,000 and 1,500 μg/L) for 30 days under controlled laboratory conditions. Natural seawater without added iron (500 μg/L) was used as reference seawater. No sediments were provided to avoid iron scavenging by particle surfaces in the sediment. We measured plant response in terms of survivorship, morphology, growth rate and productivity. Survival analysis combined with morphological, dynamic and productive assessment suggested that the optimum seawater iron concentration for the establishment of Z. marina seedlings and young plants is 700 μg/L. The no observed effect concentration, lowest observed effect concentration, lethal concentration that caused an increase in mortality to 10% of that of the control, and the effect concentration that caused a decrease in growth to 10% of that of the control values of young plants were significantly lower than those of seedlings, implying an increased sensitivity to high Fe concentrations (>1,000 μg/L). This study further develops our understanding of the physiological ecology of the early life stages of Z. marina and provides data that could prove helpful in the development of successful eelgrass restoration and conservation.     

琼东南盆地北礁地区梅山组丘形反射特征

目前琼东南盆地北礁凹陷中中新统梅山组顶部丘形反射引起广泛关注,但对其成因有不同认识。本文通过高精度二维、三维地震、钻井资料,研究丘形反射的特征。研究表明北礁地区梅山组顶部发育近东西向展布的长条形丘体,丘间为水道,丘内为中-弱振幅的地震反射,与西南部强振幅水道砂岩形成鲜明的对比,波阻抗反演揭示丘内为低波阻抗,属泥岩范畴。梅山组塑性丘内地层发生重力扩展,在其上覆的脆性地层（强振幅砂岩和弱振幅泥岩）发育多边形断层,反推出梅山组形成于深水环境,丘为泥丘,沉积环境分析也认为北礁凹陷中中新世为半深海沉积,梅山组的丘-谷分别对应上覆地层的谷-丘,认为是底流剥蚀/沉积成因。本文的研究对南海北部丘形反射的认识有重要意义,并可降低油气探勘风险。     

Expected Sliding Distance of Vertical Slit Caisson Breakwater

Evaluating the expected sliding distance of a vertical slit caisson breakwater is proposed. Time history for the wave load to a vertical slit caisson is made. It consists of two impulsive wave pressures followed by a smooth sinusoidal pressure. In the numerical analysis, the sliding distance for an attack of single wave was shown and the expected sliding distance during 50 years was also presented. Those results were compared with a vertical front caisson breakwater without slit. It was concluded that the sliding distance of a vertical slit caisson may be over-estimated if the wave pressure on the caisson is evaluated without considering vertical slit.     

Succession and seasonal variation in epilithic biofilms on artificial reefs in culture waters of the sea cucumber <Emphasis Type="Italic">Apostichopus japonicus</Emphasis>

Periphytic biofilms in aquaculture waters are thought to improve water quality, provide an additional food source, and improve the survival and growth of some reared animals. In the Asia- Pacific region, particularly in China, artificial reefs are commonly used in the commercial farming of sea cucumbers. However, few studies have examined the epilithic biofilms on the artificial reefs. To gain a better understanding of the succession of epilithic biofilms and their ecological processes in sea cucumber culture waters, two experiments were conducted in culture waters of the sea cucumber Apostichopus japonicus in Rongcheng, China, using artificial test panels. On the test panels of succession experiment, more than 67 species were identified in the biofilms. On the test panels of seasonal variation experiment, more than 46 species were recorded in the biofilms. In both experiments, communities of epilithic biofilms were dominated by diatoms, green algae and the annelid Spirorbis sp. In the initial colonization, the dominant diatoms were Cocconeis sp., Amphora spp. and Nitzschia closterium in June, which were succeeded by species of Navicula, Cocconeis and Nitzschia (July to September), and then by Licmophora abbreviata, Nitzschia closterium and Synedra spp. in the following months. A diatom bloom in the autumn and filamentous green algae burst in the summer were also observed. Ecological indices well annotated the succession and seasonal changes in epilithic communities. Multidimensional scaling (MDS) analysis found significant differences in diatom community composition among months and seasons. Fast growth of biofilms was observed in the summer and autumn, whereas the biomass of summer biofilms was largely made up of filamentous green algae. Present results show that the components of epilithic biofilms are mostly optimal foods of A. japonicus, suggesting that biofilms on artificial reefs may contribute important nutritional sources for sea cucumbers during their growth seasons. Future works should include quantitative determination of the contribution of epilithic biofilms to the diet of A. japonicus, potential roles of epilithic biofilms in regulating the water quality of sea cucumber ponds, and the regulation of epilithic biofilms in sea cucumber culture ponds.     

农牧交错带农田土壤风蚀PM10释放特征

粉尘释放是风蚀造成危害的一个重要过程。以农牧交错带沙区和非沙区农田土壤为研究对象,利用室内风洞模拟实验,实时监测了风蚀过程中释放的PM₁₀,分析了PM₁₀的动态变化特征,以深入认识土壤风蚀粉尘释放机理。结果表明：非沙区农田土壤风蚀强度远低于沙区农田,与风速呈指数函数关系;非沙区农田的土壤粉尘释放在不同风速下均以气流直接抬升模式为主,平均PM₁₀通量与风速呈线性函数关系,最大PM₁₀通量与风速呈幂函数关系;沙区农田的土壤粉尘释放在风速增大到一定程度后呈气流直接抬升和砂粒跃移冲击复合模式,最大PM₁₀通量增加不明显,但平均PM₁₀通量明显高于非沙区农田;对于沙区和非沙区农田而言,平均PM₁₀通量与风蚀速率呈对数函数关系。