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141.
目的:研究卫矛醇对C6胶质瘤细胞株的体外增殖及凋亡的影响。方法:将C6大鼠胶质瘤细胞随机分为对照组、卫矛醇干预组、卫矛醇+雷帕霉素组、卫矛醇+3-MA组,各组予不同药物孵育,采用MTT法检测卫矛醇对C6细胞的体外增殖抑制率,筛选出抑制率相对较高且生长状态良好的细胞;应用Hoechest 33342染色透射电镜观察细胞凋亡亚显微结构的改变,采用免疫荧光和Western Blot技术检测与凋亡和自噬相关蛋白的表达水平。结果:卫矛醇对C6胶质瘤细胞增殖有明显的抑制作用,并呈现出良好的浓度-效应相关性,与对照组比较,随着浓度的增加,细胞的增殖抑制率上升(P<0.01);Hoechst染色结果显示,与对照组相比,共同孵育卫矛醇24 h后的C6细胞出现明显的核固缩现象,证明有大量的细胞出现凋亡;Western Blot结果显示,与对照组相比,促凋亡基因Bax表达上调,抗凋亡基因Bcl-2表达下调。结论:卫矛醇能够抑制C6细胞的增殖,并诱导其凋亡,其作用机制可能与上调Bax、下调Bcl-2有关。 相似文献
142.
城市绿地作为城市生态系统中不可缺少的一部分,也是城市建设中必须考虑的因素之一,如何更有效地得到城市绿地的分布状况对城市的建设有着深刻的意义.高分辨率遥感影像如今已经成为城市绿地调查的重要数据.为了探究最适用于高分影像中城市绿地自动提取的方法,以南京市某区域为例,以高分二号高分辨率影像为数据源,分别使用了监督分类、利用归一化植被指数(NDVI)分类和基于规则的面向对象分类这三种信息自动提取方法,并将得到的分类结果与精度评价进行了对比分析,结果表明,基于规则的面向对象分类方法的精度明显高于其他两种方法. 相似文献
143.
144.
145.
本文运用地理学和物理学的规律进行论证,认为楚义芳同志在《地理学的逻辑方法与基本法则》一文中提出的“地理学公理”不能成立。并在此基础上进一步阐明一个公理体系不适合于地理学,而经验假设则是绝对必要的。 相似文献
146.
Sulfur Isotope Fractionation in Magmatic Systems:Models of Rayleigh Distillation and Selective Flux 总被引:2,自引:0,他引:2
郑永飞 《中国地球化学学报》1990,9(1):27-45
The effect of Rayleigh distillation by outgassing of SO2 and H2S on the isotopic composition of sulfur remaining in silicate melts is quantitatively modelled.A threshold mole fraction of sulfur in sulfide component of the melts is reckoned to be of critical importance in shifting the δ^34S of the melts mith respect to the original magmas.The partial equilibrium fractionation in a magmatic system is evaluated by assuming that a non-equilibrium flux of sulfur occurs between magmatic volatiles and the melts,while an equilibrium fractionation is approached between sulfate and sulfide within the melts.The results show that under high fo2 conditions,the sulfate/sulfide ratio in a melt entds to increase,and the δ^34S value of sulfur in a solidified rock might then be shifted in the positive direction.This may either be due to Rayleigh outgassing in case the mole fraction of sulfide is less than the threshold,or due to a unidirectional increase in δ^34S value of the sulfate with decreaing temperature,Conversely,at low fo2,the sulfate/sulfide ratio tends to decrease and the δ^34S value of total sulfur could be driven in the negative direction,either because of the Rayleigh outgassing in case the mole fraction of sulfide is greater than the threshold,or because of a unidirectional decrease inδ^34S value of the sulfide.To establish isotopic equilibrium between sulfate and sulfide,the HM,QFM or WM buffers in the magmatic system are suggested to provide the redox couple that could simultaneously reduce the sulfate and oxidize the sulfide.CaO present in the silicatte Melts is also called upon to participate in the chemical equilibrium between sulfate and sulfide,Consequently,the δ^34S value of an igneous rock could considerably deviate from that of its original magma due to the influence of oxygen fugacity and temperature at the time of magma solidification. 相似文献
147.
Y. -F. Zheng 《Mineralium Deposita》1990,25(4):246-250
The effect of the outgassing of CO2 from a hydrothermal fluid on the C- and O-isotopic compositions of calcite, which is precipitated from this fluid, is quantitatively modelled in terms of batch and Rayleigh distillation equations. Both CO2 degassing and calcite precipitation are considered to be the removal mechanisms for dissolved carbon species from the fluid. Combined degassing-precipitation models are then developed by taking H2CO3 and HCO
3
–
, respectively, as the dominant dissolved carbon species. A positive correlation array between
13C and
13O values of calcite can be yielded by the precipitation of calcite from a H2CO
3
–
-dominant fluid, accompanied by a progressive decrease in temperature during CO2 degassing, whereas calcite precipitated from a HCO
3
–
-dominant fluid under the same conditions tends to display much smaller variation in
13C values than in
18O values. The combined processes of CO2 degassing and calcite precipitation result in lowering the
13C value of calcites with respect to those precipitated in a closed system simply due to temperature effect. Carbon and oxygen isotopic data for calcite from the Kushikino gold-mining area in Japan illustrate the application of quantitative modelling, and degassing of CO2 is suggested as a more likely cause for the precipitation of the calcite and quartz in this mining area. 相似文献
148.
We find that the profile changes observed for the UV emission lines L,Civ 1549, and MgIII 2798, in the Seyfert 1 galaxy Fairall 9 occur mainly near the line centre for L andCiv and in the wings for MgIII. These results indicate that the broad line region (BLR) has a complex structure, possibly with non-spherical components.Paper presented at the 11th European Regional Astronomical Meetings of the IAU on New Windows to the Universe, held 3–8 July, 1989, Tenerife, Canary Islands, Spain. 相似文献
149.
U-Pb, Hf and O isotope evidence for two episodes of fluid-assisted zircon growth in marble-hosted eclogites from the Dabie orogen 总被引:9,自引:0,他引:9
Yuan-Bao Wu Yong-Fei Zheng Zi-Fu Zhao Bing Gong Fu-Yuan Wu 《Geochimica et cosmochimica acta》2006,70(14):3743-3761
A combined study of internal structure, U-Pb age, and Hf and O isotopes was carried out for metamorphic zircons from ultrahigh-pressure eclogite boudins enclosed in marbles from the Dabie orogen in China. CL imaging identifies two types of zircon that are metamorphically new growth and recrystallized domain, respectively. The metamorphic zircons have low Th and U contents with low Th/U ratios, yielding two groups of 206Pb/238U age at 245 ± 3 to 240 ± 2 Ma and 226 ± 4 to 223 ± 2 Ma, respectively. Anomalously high δ18O values were obtained for refractory minerals, with 9.9 to 21.4‰ for garnet and 16.9‰ for zircon. This indicates that eclogite protolith is sedimentary rocks capable of liberating aqueous fluid for zircon growth during continental subduction-zone metamorphism. Most of the zircons are characterized by very low 176Lu/177Hf ratios of 0.000001-0.000028, indicating their growth in association with garnet recrystallization. A few of them falling within the older age group have comparatively high 176Lu/177Hf ratios of 0.000192-0.000383, suggesting their growth prior to the formation of garnet in the late stage of subduction. The variations in the Lu/Hf ratios for zircons can thus be used to correlate with garnet growth during eclogite-facies metamorphism. In either case, the zircons have variable εHf (t) values for individual samples, suggesting that their protolith is heterogeneous in Hf isotope composition with localized fluid availability in the bulk processes of orogenic cycle. Nevertheless, a positive correlation exists between 206Pb/238U ages and Lu-Hf isotope ratios for the metamorphically recrystallized zircons, suggesting that eclogite-facies metamorphism in the presence of fluid has the identical effect on zircon Lu-Hf and U-Th-Pb isotopic systems. We conclude that the zircons of the older group grew in the presence of fluid during the subduction prior to the onset of peak ultrahigh-pressure metamorphism, whereas the younger zircons grew in the presence of fluid released during the initial exhumation toward high-pressure eclogite-facies regime. 相似文献
150.