Identification of five sea cucumber species through PCR-RFLP analysis

Sea cucumbers are traditional marine food and Chinese medicine in Asia. The rapid expansion of sea cucumber market has resulted in various problems, such as commercial fraud and mislabeling. Conventionally, sea cucumber species could be distinguished by their morphological and anatomical characteristics; however, their identification becomes difficult when they are processed. The aim of this study was to develop a new convenient method of identifying and distinguishing sea cucumber species. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of mitochondrial cytochrome oxidase I gene (COI) was used to identifing five sea cucumber species (Apostichopus japonicus, Cucumaria frondosa, Thelenota ananas, Parastichopus californicus and Actinopyga lecanora). A 692 bp fragment of COI was searched for BamHI, KpnI, PstI, XbaI and Eco31I restriction sites with DNAMAN 6.0, which were then used to PCR-RFLP analysis. These five sea cucumber species can be discriminated from mixed sea cucumbers. The developed PCR-RFLP assay will facilitate the identification of sea cucumbers, making their source tracing and quality controlling feasible.     

CORROSION AND ADHERENCE AT SEA OIL-PLATFORM

Measurements of the adhesive corrosion and protective potentials of 3 Bohai Sea oilplatforms under different cathodic protection showed corrosion still occurred on these platforms undertheir designed protective potentials. Preliminary conclusions from indoor experiments on the relation betweenadhesive corrosion and cathodic protection showed adhering organisms caused the corrosion onthese platforms.     

Spatial correlation and ecological characteristics analysis of management area for biodiversity conservation and relevant regionalization

Formulation of different ecological zone plans according to the corresponding protection targets and the necessity of proper conservation policy is one of the measures to achieve the goal of ecological conservation in China.In order to clarify the interrelation among key ecological zone plans,this paper carried out the research on spatial relation of priority areas of biodiversity conservation and three key ecological areas（key ecological function areas,key regions of ecological service function,national nature reserves） and the research on ecological conditions,based on multi-scale ecological spatial theme information,which incorporates elements like ecologi-cal quality and type,and by the aid of spatial information analysis and GIS modeling.The results showed a contrastively fine spatial consistency with 68.8% of priority areas of biodiversity conservation overlapping with three key ecological areas.Although the envi-ronment in priority areas of biodiversity conservation were in good conditions,protection pressure is also increasing,powerful supervi-sion and protection should not be ignored.The environmental conditions in the overlapping areas,as a whole,were superior to those in the non-overlapping areas.Since two areas have different characteristics,targeted protection measures should be formulated based on this difference,which will be very important for biodiversity conservation in priority areas of biodiversity conservation.     

中国地壳运动观测网络中的GPS数据流

介绍了中国地壳运动观测网络中的 GPS数据结构 ,对 GPS及其他观测数据的数据转换、数据传输、数据处理、管理及数据共享等工作流程作了详细说明。     

双棘黄姑鱼血清性类固醇激素的周年变化

采用放射免疫法(RIA)测定双棘黄姑鱼(Nibea diacanthus)血清性类固醇激素的周年变化。结果表明:雌鱼血清雌二醇(Estadiol-17β)浓度一年出现两次峰值,均在成熟产卵前一个月,即4月[(338±59)pg/mL]和9月[(296±48)pg/mL];血清睾酮(T)浓度在卵黄发生期急剧上升,并在卵巢成熟期达到最高值,分别是5月(2.506±0.262 ng/mL)和10月份(2.268±0.243 ng/mL),分别在血清雌二醇浓度两次峰值出现1个月之后,因此,雌鱼的血清雌二醇浓度和血清睾酮浓度均与性腺发育相关。雄鱼血清睾酮浓度的两次峰值均出现在性腺已发育成熟的5月份(2.307±0.792 ng/mL)和10月份(2.286±0.607 ng/mL),与性腺发育有明显的相关性;而血清雌二醇浓度全年维持在一个相对稳定的水平,与雄性性腺发育相关性不明显。     

浅谈土地档案在土地管理中的作用

土地档案是在国土资源实践活动中直接形成的具有保存价值并归档保存的文字、图纸、图表、航卫片、电子文件、声像材料等。近年来,在转变国土资源利用方式和深化国土资源管理改革的新形势下,在转变政府职能,实行窗口办文,限时办结各类报件的新情况下,土地档案在国土资源业务活动     

一种新型数字水准仪室内检定装置

数字化精密水准测量仪器在水准及形变测量领域中起着极其重要的作用,而对数字水准仪的计量检定工作是使其正常运行的前提,针对其检定项目室内化的难题,介绍了一种国产新型数字水准仪的室内检定装置.     

Study on changes of plasmalemma permeability and some primary inorganic ions of Antarctic ice microalgae （Chlamydomonas sp. ICE-L） in the low-temperature stress

The changes of plasmalemma permeability and some primary inorganic ions of Antarctic ice microalgae （ Chlamydomonas sp. ICE-L） in the low-temperature stress were examined. The plasmalemma of 1CE-L could maintain the stability at the freezing condition of -6 ℃. That signifies that it could maintain the proper function of plasmalemma and stability of the intracellular environment during sea ice formation. The function of inorganic ions on low-temperature adaptation of ICE-L was investigated by using the X-ray microanalysis method. Low temperature （0 - -6 ℃ ） induces Ca^2 ＋ concentration increment of cytoplasm, but after 24 h the content decrease quickly to normal value. As a matter of fact, Ca^2 ＋ plays an important role as the second messenger in the low temperature adaptation of ICE-L. In addition, low temperature also influences on the other primary inorganic ions transfer and the cell maintains activity by keeping ratio balance among different ions. Above all, it is necessary for Antarctic ice microalgae to survive and breed by maintaining the stability of K^ ＋ content and the balance of Na^ ＋/Cl^ -.     

西沙群岛的现代有孔虫Ⅱ

本文为我国西沙群岛现代有孔虫研究报告的第二部分。 笔者在《西沙群岛的现代有孔虫Ⅰ》一文中已报道了175种。本文共记述了底栖有孔虫240种(包括6个未定种)，其中的235种为首次记录，分隶于3亚目，10总科，38科，110属;有8个新属和86个新种。砂质胶结型种类有31种，占总种数的13.2%；钙质无孔型种类有48种，占总种数的20.4%；钙质有孔型种类有156种，占总种数的66.4%；浮游有孔虫未见有新增加的种类。凡是在前文己报道过的属以及本文重复报道的5个种，均不再加以描述。新种的模式标本均保存于中国科学院海洋研究所。 通过本文对现有的56份砂样所含有孔虫的分析鉴定，可以看出西沙群岛的有孔虫区系是极其丰富的。随着今后更全面的调查，特别是水下样品的进一步补充采集，待发现其他种类时，将另文报道。     

中亚造山带东南部晚古生代-早中生代地壳增生和古亚洲洋演化:来自内蒙古东南部林西-东乌旗地区岩浆岩的证据SCIEI北大核心CSCD

本文在系统收集内蒙古林西-东乌旗地区晚古生代-早中生代岩浆岩的年代学、岩石地球化学以及锆石Hf同位素资料基础上,通过分析岩浆岩岩石组合随时空的变化规律,并结合区域地质资料,探讨了中亚造山带东南部洋盆演化和地壳增生等重要地质问题。研究结果表明,二连浩特-贺根山蛇绿岩带南、北两侧晚古生代-早中生代岩浆岩在年代学上显示不同的活动期次,具有不同岩石组合和地球化学特征,指示它们分属于不同的构造岩浆岩带。蛇绿岩带以北晚泥盆世-中二叠世岩浆活动在时间上呈连续分布的特征,并在晚石炭-早二叠世时期达到活动峰值。火成岩构造组合分析表明,晚泥盆世-石炭纪和早-中二叠世岩浆活动分别与二连浩特-贺根山洋盆向乌里雅斯太大陆边缘之下的俯冲和洋盆闭合后俯冲板片断离引起的软流圈上涌造成的区域伸展背景有关。蛇绿岩带以南岩浆活动时间上呈现石炭纪、早-中二叠世、晚二叠世-三叠纪幕式分布特征,各期岩浆活动前锋有随时间向南迁移的趋势。这三期岩浆活动分别与古亚洲洋板片向宝力道岛弧之下的俯冲、板片后撤以及洋盆消失之后古板块的碰撞造山作用有关。锆石Hf同位素分析表明,中亚造山带东南部晚古生代至早中生代时期存在显著的地壳增生;其中二连浩特-贺根山蛇绿岩带以北表现为地壳的垂向增生,以南表现为地壳的侧向增生。