Screening of extraction methods for glycoproteins from jellyfish (Rhopilema esculentum) oral-arms by high performance liquid chromatography

In order to select an optimum extraction method for the target glycoprotein (TGP) from jellyfish (Rhopilema esculentum) oral-arms, a high performance liquid chromatography (HPLC)-assay for the determination of the TGP was developed. Purified target glycoprotein was taken as a standard glycoprotein. The results showed that the calibration curves for peak area plotted against concentration for TGP were linear (r = 0.9984, y = 4.5895x+47.601) over concentrations ranging from 50 to 400 mgL-1. The mean extraction recovery was 97.84% (CV2.60%). The fractions containing TGP were isolated from jellyfish (R. esculentum) oral-arms by four extraction methods: 1) water extraction (WE), 2) phosphate buffer solution (PBS) extraction (PE), 3) ultrasound-assisted water extraction (UA-WE), 4) ultrasound-assisted PBS extraction (UA-PE). The lyophilized extract was dissolved in Milli-Q water and analyzed directly on a short TSK-GEL G4000PWXL (7.8 mm×300 mm) column. Our results indicated that the UA-PE method was the optimum extraction method selected by HPLC.     

The First Solar Seeing Profile Measurement with Two Apertures and Multiple Guide Regions

The sky brightness is a critical parameter for estimating the coronal observation conditions for a solar observatory. As part of a site-survey project in Western China, we measured the sky brightness continuously at the Lijiang Observatory in Yunnan province in 2011. A sky brightness monitor (SBM) was adopted to measure the sky brightness in a region extending from 4.5 to 7.0 apparent solar radii based on the experience of the Daniel K. Inouye Solar Telescope (DKIST) site survey. Every month, the data were collected manually for at least one week. We collected statistics of the sky brightness at four bandpasses located at 450, 530, 890, and 940 nm. The results indicate that aerosol scattering is of great importance for the diurnal variation of the sky brightness. For most of the year, the sky brightness remains under 20 millionths per airmass before local Noon. On average, the sky brightness is less than 20 millionths, which accounts for 40.41% of the total observing time on a clear day. The best observation time is from 9:00 to 13:00 (Beijing time). The Lijiang Observatory is therefore suitable for coronagraphs investigating the structures and dynamics of the corona.     

虚拟天文台的技术进展

虚拟天文台是在望远镜和探测器的研制技术、计算机网络技术取得突破性进展的条件下产生的，与最新的信息技术紧密结合成为其发展最鲜明的特点。用可扩展标记语言（简称XML）技术封装天文数据，把虚拟天文台建立在网格的体系结构之上是当今虚拟天文台技术发展的主流。按照目前虚拟天文台具代表性的层次式体系结构分层介绍各个层次的相关技术，同时提出充分利用现有资源优势实施中国虚拟天文台计算的设想。     

Annual cycle and budgets of nutrients in the Bohai sea

The environmental problems in the Bohai Sea have become more serious in the last decade. High nutrient concentration contributes much to it. A Sino-German cooperation program has been carried out to improve the understanding of the ecosystem by observations and modelling. A three-dimensional ecosystem model, coupled with a physical transport model, is adopted in this study. The simulation for the year 1982 is validated by the data collected in 1982/1983. The simulated annual mean nutrient concentrations are in good agreement with observations. The nutrient concentrations in the bohai Sea, which are crucial to the algal growth, are high in winter and low in summer. There are depletion from spring to summer and elevation from autumn to winter for nutrients. The nutrients’ depletion is a response to the consumption of the phytoplankton bloom in spring. Internal recycle and external compensation affect the nutrient cycle. Their contributions to the nutrient budgets are discussed based on the simulated results. Production and respiration are the most important sink and source of nutrients. The process of photosynthesis consumes 152 kilotons-P and 831.1 kilotons-N while respiration releases 94.5 kilotons-P and 516.6 kilotons-N in the same period. The remineralization of the detritus pool is an important source of nutrient regeneration, It can compensate 23 percent of the nutrient consumed by the production process. The inputs of phosphates and nitrogen from rivers are 0.55 and 52.7 kilotons respectively. The net nutrient budget is −3.05 kilotons-P and 31.6 kilotons-N.     

The Orbital Evolution of Two Sounding Satellites and Analysis of the Accuracy of Orbit Determination

The satellites TC-1 and TC-2 are the two Chinese satellites with great elliptical orbits which are still in orbit around the earth at present. Since the launch the orbits of the two satellites have continuously evolved, which has a certain effect on the orbit determination and prediction precision. The regularities of the orbital evolution of the two sounding satellites are qualitatively and quantitatively analyzed. Under the current tracking mode the corresponding prediction precision of orbit determination is analyzed based on the different stages of the orbital evolution, thereby providing the basis for the adjustment of planning mode by the satellite application departments and the guarantee of normal satellite payload. Finally, the orbital lifetimes of the two satellites are predicted through the trend of the orbital evolution.     

Formation flying in elliptic orbits with the J2 perturbation

Relative dynamics between the chief satellite and the deputy ones in formation flying is crucial to maintaining the formation. A good choice of the formation usually requires a lower control frequency or less control energy. For formation flying missions in highly elliptic orbits, the well-known C-W equation is not accurate enough. Instead, Lawden’s equation is often used. First, the solution to Lawden’s equation with a very simple form is deduced. Then the J2 perturbation is added. It is found that Lawden’s solution is not necessarily valid when the J2 perturbation is considered. Completely discarding Lawden’s solution and borrowing the idea of mean orbit elements, two rules to initialize the formation are proposed. The deviation speed is greatly reduced. Different from previous studies on the J2 perturbation, except for the relatively simple expression for the semi-major axis, the tedious formulae of the long period terms and the short period terms of other orbital elements are not used. In addition, the deviation speed is further reduced by compensation of the nonlinear effects. Finally, a loose control strategy of the formation is proposed. To test the robustness of this strategy, a third body perturbation is added in numerical simulations.     

Optimization of Low-Thrust Trajectories Using an Indirect Shooting Method without Guesses of Initial Costates

According to the optimal control theory, the optimal control problem of the low-thrust tra jectory can be converted into a solution of nonlinear two- point boundary-value problem (TPBVP). To solve the TPBVP, the repeated random guesses for the initial costate variables and iterative computations are needed. In order to enhance the convergence of the iterations, we select an appropriate performance index, and then linearize the equations of the TPBVP around a Keplerian orbit. For multi-revolution transfers, instead of the multi- revolution Lambert tra jectory, multiple segmented Keplerian arcs are used to ensure the effectiveness of the linearization. The method is totally automatic with multiple iterations. With this method, we can get the results within 3 ∼ 5 iterations, and the random guess of the initial costates is unnecessary. Finally by the iterative optimization of the performance index, a better control strategy approaching to the bang-bang control is obtained.     

JCXA——733型电子探针快速半定量分析程序

在近代科学技术发展前缘之一的微束分析中,电子探针X射线显微分析仪是目前相当活跃的微区微量物质成份分析的设备.六十年代初期,电子探针开始被应用于地质领域进行矿物成份分析,至今它已成为矿物岩石学,地球化学、矿床综合评价及综合利用等众多科研课题研究的重要手段.我们使用的JCXA-733型电子探针装配的是美国DEC公司PDF-11型电子计算机,RX02双软盘驱动装置、LA-36型宽行打字机,RT-1l操作系统     

THE DIVERGENCE-CONVERGENCE EFFECT OF SURFACE CURRENT AND ITS ACTION ON THE BOHAI SEA

The action of the wind field and the influence of topography can cause divergence or convergence of surface current. The existence of the divergence-convergence effect is proved and the dynamical significance of the divergent or convergent state and its link with many marine phenomena are pointed out. Divergence fields of surface current in the Bohai Sea in winter and summer are obtained by numerical modelling describing the divergence-convergence character of seasonally wind-driven current. The relation between the effect and seasonal marine phenomena is discussed. Study on the divergence-convergence effect of surface current (DCESC)can be an indirect method for testing the calculated results.     

松科二井早白垩世早期玄武安山岩的发现及地质意义

松科二井超深钻探获取了松辽盆地连续沉积-火山岩心,为系统研究中生代松辽盆地形成机制、揭示中国东北地区构造演化历史提供了极好的研究材料。本文对取自松科二井井深-6035~-6084m的玄武安山岩开展了系统的锆石U-Pb定年、全岩主微量元素和Sr-Nd同位素分析测试。研究结果表明,松科二井玄武安山岩隶属于火石岭组底部,其形成时代为早白垩世早期（141.6±1.4Ma）。玄武安山岩样品富集大离子亲石元素,亏损高场强元素,具有弧岩浆的地球化学特征。这些样品显示了一定程度的轻重稀土元素分异（（La/Yb）_N=8.40~11.88）,具有轻微亏损的Sr-Nd同位素特征（（⁸⁷Sr/⁸⁶Sr）_i=0.70496~0.70478,ε_Nd（t）=1.05~1.61）,表明它们很可能源自于岩石圈地幔。早白垩世时期中国东北地区主要受控于蒙古-鄂霍茨克洋的南向俯冲和东侧古太平洋俯冲过程,目前研究揭示了前者影响了同期的大兴安岭地区的火山活动,但其影响范围是否延伸至松辽盆地内部尚不清楚。古太平洋岩石圈开始俯冲后很可能诱发了松辽盆地西侧晚中生代火山岩的形成。早白垩世松辽盆地演化主要受蒙古-鄂霍茨克洋还是古太平洋板块的活动影响仍存在较多争议。松科二井玄武安山岩具有与板内玄武岩相近的Th/Hf比值（1.10~2.87）及Zr/Y比值（10.1~18.7）,表明它们具有板内岩浆的地球化学特征,且松科二井样品比同时代盆地西部的大兴安岭地区玄武岩具有更高的Nb/Ta比值,更高的Zr、Hf与Ti含量,以及更亏损的Nd同位素特征,可能指示与大兴安岭地区玄武岩形成于不同的构造背景。松辽盆地内早白垩世火山岩很可能为古太平洋板片回转诱发形成,指示早白垩世古太平洋板片回转诱发的岩石圈伸展促进了松辽盆地的形成。