Comparative analysis on microbial community associated with different gastrointestinal regions of wild northern snakehead Channa argus Cantor, 1842

Microbial communities in different gastrointestinal regions(stomach, foregut, midgut, and hindgut) of the northern snakehead C hanna argus(Cantor, 1842) were compared by polymerase chain reaction and partial 16 S r DNA sequencing. A total of 194, 140, 212, and 122 OTUs were detected in the stomach, foregut, midgut, and hindgut, respectively. Significant dif ferences were found in the Sobs, ACE, Shannon, and Simpson indices among samples(P 0.05). The gastrointestinal microbial community of C. argus consisted predominantly of Proteobacteria with either H alomonas, Shewanella, Plesiomonas, or Sphingomonas. Fusobacteria, Firmicutes, and Bacteroidetes also existed in the gastrointestinal tracts. However, significant diff erences were found in the compositions of microbial community among the four regions( P 0.05). Cyanobacteria and Spirochetes were significantly higher in the midgut and hindgut( P 0.05). Fusobacteria and Firmicutes were dominant in the hindgut and foregut, respectively(P 0.05). Proteobacteria was the lowest in the hindgut( P 0.05). At genus level, C etobacterium and Plesiomonas were significantly higher in the hindgut than in the other three samples( P 0.05). Clostridium and Prevotella were the highest in the midgut(P 0.05). Halomonas, Shewanella, and S phingomonas were the highest in the foregut( P 0.05). Paracoccus and Vibrio were the highest in the stomach. Several genera were only detected in certain regions, as follows: stomach, P aracoccus and Vibrio; foregut, Halomonas, Shewanella, and Sphingomonas; midgut, Clostridium and Prevotella; and hindgut, C etobacterium and Plesiomonas( P 0.05). At the species level, A cinetobacter rhizosphaerae was only detected in the stomach. P revotella copri and Clostridium perfring were not detected in the foregut and midgut, respectively, whereas Prevotella copri and Faecalibacterium pra were not detected in the hindgut. These findings provide valuable information on the microbial community in each gastrointestinal region of C. argus. Moreover, this study indicated that microbial community was not only related to rearing environment but also to the physico-chemical characteristics of each gastrointestinal region.     

农村居民点动态演变及驱动机制研究——以江苏省宜兴市为例

选取宜兴市为研究区,在GIS和RS技术的支撑下,对农村居民点用地的规模变化和空间分布进行分析,对引起农村居民点用地变化的驱动因素进行研究。结果表明:1农村居民点用地整体呈下降趋势,变化速度逐年减慢,主要新增来源是农用地,主要转移去向是城镇建设用地;2在地形平坦、距离水源和农用地较近的地区,农村居民点分布密集;3经济发展、人口减少、交通改善和城市化水平提高是影响农村居民点变化的主要驱动因素。     

毫秒时间尺度太阳微波辐射新特征

自1990年6月北京天文台太阳射电偏振仪投入实测以来,观测到一百多个“Spike”和短时标精细结构微波幅射,其中包括一些新的微波幅射特征。本文给出了主要的四点特征:(1)窄带辐射;(2)快速偏振逆转;(3)快速频率漂移;(4)不同时标的准周期振荡。     

巴丹吉林沙漠南缘的植物固沙问题

在野外考察的基础上,选择13个典型样地,研究了巴丹吉林沙漠南缘植被组成、群落特征和植被格局。结果表明：调查的样地中有荒漠植物31种,其中灌木12种,多年生草本11种,一二年生草本8种。种群密度3 000—14 000株·hm^-2,优势种在群落中地位显著,群落稳定性较差;植被斑块面积小、最大斑块指数低。沙漠南缘年降水量虽然仅100 mm左右,但有霸王（Zygophyllum xanthoxylon）、红砂（Reaumuria soongarica）和沙蒿（Artemisia desertorum）等灌木和半灌木天然植物群落分布,有白刺（Nitraria tangutorum）和膜果麻黄（Ephedra przewalskii）灌丛沙堆,沙丘上生长着沙拐枣（Calligonum mongolicum）、沙鞭（Psammochloa villosa）等种群生存,且在沙漠延伸带流动沙丘上已成功建立了梭梭（Haloxylon ammodendron）人工固沙植被,表明在巴丹吉林沙漠南缘建立以乡土植物为主的固沙植被是可行的。在未来研究中,应在山水林田湖草沙生命共同体理念下加强从区域尺度上解析水、植被和沙的关系,系统了解生态系统的原真性、完整性和连通性及生态系统稳定性维持等问题,优化生态景观格局,确定生态保护目标和建设规模,同时要加强对乡土植物适应干旱风沙机制和扩繁保育技术研究,以便为区域生态建设和生态保护提供科技支撑。     

陕西黄土高原人工林土壤干层及形成原因

1 Introduction Abroad, a lot of research on soil water removal, seeping and evaporation has been done (Ole, 1998; Rapp, 2000). A seeping model of soil water was suggested (Yuin, 1998) and it was known that water removal is very slow in the unsaturated zon…     

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南京市城市居民对乡村旅游发展的感知及旅游行为研究

运用问卷调查法和基于SPSS软件的统计分析法,以南京市为例,从城市居民感知视角考察乡村旅游的发展情况。从旅游偏好、旅游频率和空间分布等方面探讨城市居民的乡村旅游行为特征,并进一步分析了乡村旅游的知晓度、居民对各乡村旅游点开发水平的感知与其空间分布之间的相关关系,以及居民对乡村旅游发展总体印象与旅游频率之间的相关关系。在此基础上,提出南京市乡村旅游发展的有关对策与建议。     

西藏措勤地区典中组火山岩同位素特征及地质意义

西藏冈底斯岩浆岩带西段北侧的林子宗群火山岩含有从板块俯冲机制向大陆碰撞机制转变的丰富信息。将措勤地区典中组火山岩与冈底斯岩浆岩带东段林周盆地的典中组火山岩在同位素地球化学、演化特征方面进行对比,探讨冈底斯岩浆岩带西段与东段在演化过程中的异同性。结果表明,措勤地区典中组火山岩的87Sr/86Sr初始比值变化范围为0.710352~0.713423,具较高的87Sr/86Sr比值;143Nd/144Nd初始比值为0.512293~0.512407,具较低的143Nd/144Nd比值;林周盆地典中组火山岩的87Sr/86Sr初始比值为0.7057~0.7063之间,143Nd/144Nd初始比值范围为0.5125~0.5126。同位素特征显示措勤地区典中组源区物质可能来自于富集Ⅱ型地幔,即源区为俯冲和再循环的大陆地壳物质与地幔岩发生了混合作用的产物,源区受控于冈底斯地壳基底。林周盆地典中组火山岩源区物质可能与富集Ⅰ型地幔有关。     

BAC end sequencing of Pacific white shrimp <Emphasis Type="Italic">Litopenaeus vannamei</Emphasis>: a glimpse into the genome of Penaeid shrimp

Little is known about the genome of Pacific white shrimp (Litopenaeus vannamei). To address this, we conducted BAC (bacterial artificial chromosome) end sequencing of L. vannamei. We selected and sequenced 7 812 BAC clones from the BAC library LvHE from the two ends of the inserts by Sanger sequencing. After trimming and quality filtering, 11 279 BAC end sequences (BESs) including 4 609 paired- ends BESs were obtained. The total length of the BESs was 4 340 753 bp, representing 0.18% of the L. vannamei haploid genome. The lengths of the BESs ranged from 100 bp to 660 bp with an average length of 385 bp. Analysis of the BESs indicated that the L. vannamei genome is AT-rich and that the primary repeats patterns were simple sequence repeats (SSRs) and low complexity sequences. Dinucleotide and hexanucleotide repeats were the most common SSR types in the BESs. The most abundant transposable element was gypsy, which may contribute to the generation of the large genome size of L. vannamei. We successfully annotated 4 519 BESs by BLAST searching, including genes involved in immunity and sex determination. Our results provide an important resource for functional gene studies, map construction and integration, and complete genome assembly for this species.