Methods Comparison for Microsatellite Marker Development： Different Isolation Methods, Different Yield Efficiency

Microsatellite markers have become one kind of the most important molecular tools used in various researches. A large number of microsatellite markers are required for the whole genome survey in the fields of molecular ecology, quantitative genetics and genomics. Therefore, it is extremely necessary to select several versatile, low-cost, efficient and time- and labor-saving methods to develop a large panel of microsatellite markers. In this study, we used Zhikong scallop (Chlamys farreri) as the target species to compare the efficiency of the five methods derived from three strategies for microsatellite marker development. The results showed that the strategy of constructing small insert genomic DNA library resulted in poor efficiency, while the microsatellite-enriched strategy highly improved the isolation efficiency. Although the mining public database strategy is time- and cost-saving, it is difficult to obtain a large number of microsatellite markers, mainly due to the limited sequence data of non-model species deposited in public databases. Based on the results in this study, we recommend two methods, microsatellite-enriched library construction method and FIASCO-colony hybridization method, for large-scale microsatellite marker development. Both methods were derived from the mi-crosatellite-enriched strategy. The experimental results obtained from Zhikong scallop also provide the reference for microsatellite marker development in other species with large genomes.     

钻井柱状图可视化查询系统的设计与实现

钻井柱状图是石油行业勘探开发最常用的一种图件。为了开发出灵活通用的钻井柱状图制作软件,为可视化分析提供良好基础,提出了“两库四类三层”系统设计方案,应用COM思想和模板定制技术,采用C#．net语言从底层进行了软件实现,所开发的软件模块具备一定的独立性和较好的通用性。     

Hematological changes in white spot syndrome virus-infected shrimp, Fenneropenaeus chinensis (Osbeck)

The pathological changes of hemocytes in the haemolymph and hepatopancreas were examined in experimentally and naturally WSSV (white spot syndrome virus) infected Fenneropenaeus chinensis. The results showed that the pathological manifestations of hemocytes were similar among moribund shrimps infected via injection, feeding and by nature. Firstly, the total hemocyte counts (THCs) in WSSV-infected shrimp were significantly lower than those in healthy shrimp. Secondly, necrotic, broken and disintegrated cells were often observed, and a typical hematolysis was present in the haemolymph smear of WSSV-infected shrimp. Thirdly, necrosis and typical apoptosis of hemocytes were detected with TEM in the peripheral haemolymph of WSSV-infected shrimp. Hyalinocytes and semi-granulocytes with masses of WSSVs in their nuclei often appeared, whereas no granular hemocytes with WSSV were found in the hepatopancreas of moribund infected shrimps. All our results supported that hemocytes were the main target cells of WSSV, and hyalinocytes and semigranular hemocytes seemed to be more favorable for WSSV infection in F. chinensis.     

乳山湾邻近海域波浪特征要素规律研究

基于高分辨率MASNUM第三代海浪模式并根据Yuan等(2009)提出的波浪破碎统计物理模型,对乳山湾外海2008年各个季节波浪特征要素(有效波高和跨零周期等)与波浪破碎参数(白冠覆盖率、卷入深度和破碎能量损耗率)进行了数值模拟。利用Janson-1卫星高度计观测资料对有效波高模拟结果进行了检验,平均误差在0.17m左右。模拟结果显示,该海域波浪参数具有明显的季节变化特征,在2008-08乳山湾邻近海域受大风天气过程影响期间,有效波高在1.0~1.8m,破碎参数变化显著,白冠覆盖率最大达4.5%,卷入深度在1.5m以上,相应的破碎能量损耗率量值在6~11kg/s3。结果表明,波浪破碎过程对该区域海洋动力环境有着重要影响,是造成乳山湾口表层高溶解氧的可能机制之一。     

长江口表层沉积物矿物磁性分区特征及其沉积环境指示意义

本研究旨在揭示现代长江口不同沉积环境铁磁性矿物的分布差异,寻找有效识别河口-陆架沉积环境的磁学指标,以便更好地将环境磁学应用于河口古环境研究。在长江口及邻近陆架的6个沉积环境:汊道、拦门沙、三角洲前缘斜坡、前三角洲、前三角洲-陆架过渡区和残留砂区,采集表层沉积物样品,进行粒度和磁性测量。结果显示,χ和SIRM在汊道和拦门沙呈现显著高值,HIRM、χfd%、χARM、χARM/χ和χARM/SIRM在前三角洲和前缘斜坡呈现显著高值,反映了陆源物质输运距离和河口沉积动力对磁性矿物分布的控制作用。因此,参数组合HIRM、χARM、χARM/χ和χARM/SIRM可用于识别全新世地层前缘斜坡和前三角洲-陆架;参数组合χ、SIRM和S-20mT可尝试用于识别汊道和拦门沙环境。     

Effects of the seasonal variation in chlorophyll concentration on sea surface temperature in the global ocean

The effects of biological heating on the upper-ocean temperature of the global ocean are investigated using two ocean-only experiments forced by prescribed atmospheric fields during 1990–2007, on with fixed constant chlorophyll concentration, and the other with seasonally varying chlorophyll concentration. Although the existence of high chlorophyll concentrations can trap solar radiation in the upper layer and warm the surface, cooling sea surface temperature (SST) can be seen in some regions and seasons. Seventeen regions are selected and classified according to their dynamic processes, and the cooling mechanisms are investigated through heat budget analysis. The chlorophyll-induced SST variation is dependent on the variation in chlorophyll concentration and net surface heat flux and on such dynamic ocean processes as mixing, upwelling and advection. The mixed layer depth is also an important factor determining the effect. The chlorophyll-induced SST warming appears in most regions during the local spring to autumn when the mixed layer is shallow, e.g., low latitudes without upwelling and the mid-latitudes. Chlorophyll-induced SST cooling appears in regions experiencing strong upwelling, e.g., the western Arabian Sea, west coast of North Africa, South Africa and South America, the eastern tropical Pacific Ocean and the Atlantic Ocean, and strong mixing (with deep mixed layer depth), e.g., the mid-latitudes in winter.     

栉孔扇贝(Chlamys farreri) 血细胞类型及抗菌力的研究

用2种方法将栉孔扇贝血细胞分为2大类透明细胞、颗粒细胞.吉姆萨染色观察,透明细胞的细胞质中颗粒很少,细胞核圆形,居中,细胞质较少,核质比大;颗粒细胞比透明细胞大且多,染色深,但核质比小,在细胞质中有颗粒,有些颗粒细胞具双核.密度梯度离心结果,透明细胞主要位于上层,颗粒细胞则大部分位于下层.吞噬力、抗菌活力、溶菌活力及活性氧检测结果表明,颗粒细胞均强于透明细胞.     

无拉伸动校正技术在近海工程地震勘探中的应用

动校正是地震资料处理的关键内容之一,直接关系到地震资料处理结果的精度.在浅层和大偏移距情况下,常规动校正使波形发生拉伸畸变,波形拉长、频带向低频方向移动,进而影响叠加效果.通常地震资料处理中大都采用切除的方法克服动校正拉伸畸变的影响,这对目的层较深时是可取的.以工程地质调查为目的的海洋地震勘探旨在了解海底之下较浅地层深度范围内的地质信息,一般勘探区水深较浅,数据叠加道数较少,如果仍然采取切除处理,势必严重影响资料的分辨率和准确度.本文阐述了一种无拉伸动校正方法,能较好地解决动校正后的波形畸变问题,理论模型和实际资料的处理结果表明该方法在近海浅层工程地震勘探中是行之有效的,有助于提高速度分析的精度和地震资料的分辨率.     

Super resolution for astronomical observations

In order to obtain detailed information from multiple telescope observations a general blind super-resolution (SR) reconstruction approach for astronomical images is proposed in this paper. A pixel-reliability-based SR reconstruction algorithm is described and implemented, where the developed process incorporates flat field correction, automatic star searching and centering, iterative star matching, and sub-pixel image registration. Images captured by the 1-m telescope at Yunnan Observatory are used to test the proposed technique. The results of these experiments indicate that, following SR reconstruction, faint stars are more distinct, bright stars have sharper profiles, and the backgrounds have higher details; thus these results benefit from the high-precision star centering and image registration provided by the developed method. Application of the proposed approach not only provides more opportunities for new discoveries from astronomical image sequences, but will also contribute to enhancing the capabilities of most spatial or ground-based telescopes.     

凡纳滨对虾C-型凝集素LvLec2 对不同刺激的免疫应答

克隆获得了与中国明对虾(Fenneropenaeus chinensis)C-type lectin 3同源的C-型凝集素LvLec2基因序列,并通过Real-time PCR研究了其在脂多糖(lipopolysaccharide,简称LPS)、灭活溶壁微球菌(Micrococcus lysodeikticus)和白斑综合症病毒(white spot syndrome virus,简称WSSV)刺激后的转录表达变化。结果表明,LvLec2编码157个氨基酸,含有1个糖识别结构域(carbohydrate recognition domain,简称CRD),其CRD结构域中具有决定糖结合特异性的基序"EPS"(Glu118-Pro119-Ser120)序列。系统进化树分析表明LvLec2与已发表的凡纳滨对虾(Litopenaeus vannamei)C-型凝集素亲缘较远。LPS、灭活溶壁微球菌和WSSV刺激后,LvLec2基因在肝胰脏中的转录表达有明显的变化但表达模式不同。LvLec2可能作为模式识别受体参与了对虾对病原的识别或防御。