三株赤潮硅藻5.8S rDNA及转录间隔区(ITS)的克隆及序列分析

对引发赤潮的3株硅藻——1株尖刺拟菱形藻(Pseudo-nitzshia pungens)和2株中肋骨条藻(Skeletonema costatum)的5.8SrDNA和ITS(internal transcribed spacers)序列进行了PCR扩增、克隆和序列测定,并分析了硅藻门10株赤潮藻(7株从GenBank获得)的系统进化关系.研究结果表明,尖刺拟菱形藻的ITS和5.8SrDNA的长度为693bp,SK-1(分离自东海赤潮暴发区)测序得到715bp,除ITS和5.8SrDNA外,还包含部分18SrDNA和28SrDNA;SK-2(分离自青岛养殖场)的ITS和5.8SrDNA的长度为331bp,尖刺拟菱形藻与从GenBank中获得的2株尖刺拟菱形藻相似程度最高,为100%,与该属的多列拟菱形藻相似程度稍低,为82.9%.SK-2的ITS序列与SK-1的相似程度很低,只有51%,但与拟中肋骨条藻的ITS序列相似程度高,为95.5%.SK-1的ITS序列与拟中肋骨条藻的相似程度也低,为56.7%.系统进化树反映的结果与相似性反映的结果一致.研究的该株尖刺拟菱形藻从根据ITS序列研究的结果与形态鉴定的结果看是一致的;SK-2可能属于拟中肋骨条藻;SK-1比较特殊,有待于用其他的方法进一步研究确定其分类地位.     

应用荧光抗体技术检测牙鲆体内的河流弧菌

用灭活的河流弧菌(Vibrio fluvialis)抗原,注射免疫实验兔,制得凝集价为1∶5 120的抗血清。用试管凝集法检测了抗血清的特异性,再用免疫吸附法去除交叉反应,从而得到高效价、高特异性的抗河流弧菌血清。用所制备的抗血清在实验室中建立起河流弧菌荧光抗体检测技术(FAT),在荧光显微镜下可清楚地看到被标记的病原菌,整个检测过程只需3h。用河流弧菌感染牙鲆(Paralichthys olivaceus),24 h后,用FAT测定牙鲆的血液、肾脏和肝脏中的河流弧菌,在血液中河流弧菌的检出率最高,其次是肾脏,肝脏的检出率最低。以上结果表明:荧光抗体技术可以快速、灵敏、准确地检测出牙鲆体内的河流弧菌。     

Java环境下基于Hibernate的GIS数据库访问策略

全关系型数据存储模式将GIS数据的空间数据和属性数据统一存储于关系数据库中,分析Java应用程序通过JDBC和SQL语言访问关系数据库中存储的GIS数据的不足,提出基于。Hibernate的解决方案,并给出地理信息共享系统的开发实例。     

聂拉木地区高喜马拉雅岩石磁组构及其构造含义

利用岩石磁化率量值椭球体与岩石构造应变椭球体的共轴性,在缺乏岩性标志层的高喜马拉雅结晶基底（聂拉木地区）进行岩石磁组构研究,进而研究喜马拉雅造山带的变形期次和过程.磁组构特征表明：磁化率各向异性度P在樟木镇北1km处的片岩最高为172,在康山桥南1km处最低,为113;百分率各向异性度H在1182%～4507%之间;磁性线理L在102～109之间,磁性面理F在106～160之间,磁性面理F比磁性线理L发育;磁化率椭球体的形状因子T在019～076之间,磁化率椭球的扁率E在104～149之间,磁化率椭球体的形状为一压扁椭球;樟木镇北1km到肉切村,平均最小磁化率主轴方向D3为S－N（除聂拉木北1km的MA9外）,大多数样品最小磁化率主轴方向倾角I3＞51°,最高达726°,局部发育有拉长形磁化率量值椭球体.本文研究结果表明,聂拉木地区早期经历强烈的韧性变形,推测可能是一条巨大的右旋逆冲韧性变形带,晚期构造掀斜.     

速度差对井间走时层析成像质量的影响及其改进方法

在井间地震初至走时层析成像中,随着相邻地质体速度差的增大,使得射线分布不均匀,以及网格剖分不合适,导致层析成像结果不理想.物理和数值模型的井间走时层析成像表明：当速度差超过300%时,层析结果畸变较大;在300%～150%之间时,层析结果较好;低于150%时,层析结果好.在此基础上,提出了井间多尺度初至走时层析成像方法,即对同一模型采用多种网格剖分来同时进行层析成像,以获得研究区域的速度图像.数学和物理模型的井间多尺度走时层析结果表明：该方法很好地兼顾了层析成像的分辨率和精度,极大地改善了井间地震层析成像的质量.即使速度差超过30%,其多尺度的层析结果仍然较好.因此,这种方法具有实际应用的潜力.     

Early solar system aqueous activity: K isotope evidence from Allende

The alkali element K is moderately volatile and fluid mobile; thus, it can be influenced by both primary processes (evaporation and recondensation) in the solar nebula and secondary processes (thermal and aqueous alteration) in the parent body. Since these primary and secondary processes would induce different isotopic fractionations, K isotopes could become a potential tracer to distinguish them. Using recently developed methods with improved precision (0.05‰, 95% confidence interval), we systematically measured the K isotopic compositions and major/trace elemental compositions of chondritic components (18 chondrules, 3 CAIs, 2 matrices, and 5 bulks) in the carbonaceous chondrite fall Allende. Among all the components analyzed in this study, CAIs, which formed initially under high‐temperature conditions in the solar nebula and were dominated by nominally K‐free refractory minerals, have the highest K₂O content (average 0.53 wt%) and have K isotope compositions most enriched in heavy isotopes (δ⁴¹K: ?0.30 to ?0.25‰). Such an observation is consistent with previous petrologic studies that show CAIs in Allende have undergone alkali enrichment during metasomatism. In contrast, chondrules contain lower K₂O content (0.003–0.17 wt%) and generally lighter K isotope compositions (δ⁴¹K: ?0.87‰ to ?0.24‰). The matrix and bulks are nearly identical in K₂O content and K isotope compositions (0.02–0.05 wt%; δ⁴¹K: ?0.62 to ? 0.46‰), which are, as expected, right in the middle of CAIs and chondrules. This strongly indicates that most of the chondritic components of Allende suffered aqueous alteration and their K isotopic compositions are the ramification of Allende parent‐body processing instead of primary nebular signatures. Nevertheless, we propose the small K isotope fractionations observed (< 1‰) among Allende components are likely similar to the overall range of K isotopic fractionation that occurred in nebular environment. Furthermore, the K isotope compositions seen in the components of Allende in this study are consistent with MC‐ICP‐MS analyses of the components in ordinary chondrites, which also show an absence of large (10‰) isotope fractionations. This is not expected as evaporation experiments in nebular conditions suggest there should be large K isotopic fractionations. Nevertheless, possible nebular processes such as chondrules back exchanging with ambient gas when they formed could explain this lack of large K isotopic variation.     

基于GIS的寿宁县猕猴桃种植区提取

本文在对寿宁县当前经济发展和农业自然资源状况进行充分分析的基础上,根据猕猴桃的生长发育条件,确定相应的指标,应用GIS空间分析技术实现了寿宁县猕猴桃适宜种植区的自动提取.以此体现农业气候信息系统在种植区划上的可行性、优越性,并根据分析结果做出了相关的总结,以供有关部门参考.     

汞同位素地球化学研究及其在矿床学中的应用进展

汞作为一种重要的成矿元素,广泛分布于不同地质体中,并参与成岩成矿作用.随着质谱技术的飞跃发展,汞同位素地球化学研究取得引人瞩目的进展.汞同位素被广泛地应用于示踪地球表生生物地球化学过程及汞污染等.近年来,汞同位素又被应用于揭示行星的演化过程、识别地质历史时期大火成岩省及示踪矿床成矿物质来源等方面.本文在前人研究的基础上...     

Effect of different registration methods on precision of orientation based on RTK registration/georeferencing mode

Abstract

Compared with traditional methods, the three-dimensional laser-scanning (3D-LS) technique can efficiently acquire many high-quality geometric properties of rock discontinuities. In practice, engineers usually prefer to simplify the processing by using single-station point data and roughly orienting owing to the complexity of registration/georeferencing multi-station point data. However, prior published studies have paid little attention to the accuracy and reliability when determining discontinuity orientations using 3D-LS. We propose a reliable and accurate method with robust on-site applicability. As part of an ongoing effort, we are evaluating the precision of the commonly used coarse registration method and the fine registration method, and promoted the optimized coarse- and fine-registration methods and evaluated their precision. It is found that: (1) the common and the optimized registration method can meet our project’s engineering requirements, and the optimized registration method improved accuracy in the dip direction by approximately 1°; (2) fine registration using an iterative closest point (ICP) algorithm can correct both dip direction and dip angle; and (3) the orientation is of high precision with commonly used coarse and fine registration, whereas the optimization effect to correct the orientation is slightly limited.     

软土地区人工冻土无侧限瞬时抗压强度的试验研究

冻结法施工在上海隧道建设中(如隧道旁通道、地下泵房等的设计与施工)得到广泛应用,也曾引发过严重的地质灾害(如上海地铁4号线外滩段的地质灾害)。因此安全、经济、合理地将冻结法用于上海软土地区隧道建设中已经成为上海工程建设中的一个重要的研究课题。本文以上海复兴东路越江隧道旁通道冻结法施工中遇到的第⑥层粉质粘土及第⑦层粉细砂为研究对象,针对设计冻结壁重要强度参数无侧限瞬时抗压强度,进行了室内试验研究,揭示了两种土的冻结强度随温度的变化关系,同时研究了粉细砂的冻结强度随含水率的变化规律。