鳗弧菌(Vibrio anguillarum)M3菌株生长条件及其对蛋白酶产量的影响

采用体外测定细菌浓度、胞外产物(ECP)蛋白含量和蛋白酶活力的方法，进行了鳗弧菌M3菌株在2216E培养基中的培养条件研究。结果表明，该菌株用固体培养基培养至24h左右，可得到较高的菌体浓度、ECP蛋白含量和蛋白酶活力。采用响应面分析方法设计实验，用SAS统计软件分析数据，得到NaCl浓度、pH值和温度对菌体生长及蛋白酶产量影响的回归模型。在2216E培养基的基础上，添加不同氮源、碳源物质以及不同浓度蛋白胨进行生长研究。结果表明，胰大豆蛋白胨能促进菌体生长及ECP蛋白分泌；NH4Cl与酪蛋白水解物可抑制蛋白酶的产生；牙鲆肌肉匀浆对菌体、ECP蛋白产量和蛋白酶产生有不同程度的促进作用；培养基中蛋白胨浓度为4％时菌体量与ECP蛋白含量达最高值，在蛋白胨浓度为2％时蛋白酶分泌量已稳定；1％的葡萄糖、蔗糖、甘油均能显著地提高菌体及ECP蛋白产量，却抑制了蛋白酶的产生。     

冬至初春黄海暖流的路径和起源

主要根据近几年来中韩黄海水循环动力学合作调查结果,结合有关观测资料,进一步分析了冬至初春黄海暖流的路径和起源.与以往类似研究不同的主要有两点:(1)初步探讨了黄海暖流路径的季节和年际变异,并指出这种变异与北向风的强弱密切相关;(2)通过分析济州岛西侧海域混合水的去向,进一步确认了部分混合水绕济州岛运行,并进入济州海峡这一事实.同时,初步揭示进入黄海的混合水,即黄海暖流水,含有更多的东海陆架水成分.     

Effect of free surface and strut on fins attached to a strut

An extensive experimental and computational investigation of the combined and separate effects of free surface and body on the lift characteristics of a pair of fins attached to a strut and fin alone is conducted. The results reveal that the free-surface effect becomes significant when the depth of submergence to chord ratio (H/c) is less than three. The effect of the strut is also realized for shallower depth of submergence of the fins through free-surface deformation leading to a significant change in the incidence angle of the flow to the fins. The numerical results based on the Higher Order Boundary Element Method with the linearized free-surface condition show good agreement with the experimental results for fin (foil) alone even at shallow submergence, but some discrepancies appear for the fin attached to the strut at higher speeds mostly due to the neglect of the nonlinear free-surface effect.     

Fully nonlinear numerical wave tank (NWT) simulations and wave run-up prediction around 3-D structures

A finite-difference scheme and a modified marker-and-cell (MAC) algorithm have been developed to investigate the interactions of fully nonlinear waves with two- or three-dimensional structures of arbitrary shape. The Navier–Stokes (NS) and continuity equations are solved in the computational domain and the boundary values are updated at each time step by the finite-difference time-marching scheme in the framework of a rectangular coordinate system. The fully nonlinear kinematic free-surface condition is implemented by the marker-density function (MDF) technique developed for two fluid layers.To demonstrate the capability and accuracy of the present method, the numerical simulation of backstep flows with free-surface, and the numerical tests of the MDF technique with limit functions are conducted. The 3D program was then applied to nonlinear wave interactions with conical gravity platforms of circular and octagonal cross-sections. The numerical prediction of maximum wave run-up on arctic structures is compared with the prediction of the Shore Protection Manual (SPM) method and those of linear and second-order diffraction analyses based on potential theory and boundary element method (BEM). Through this comparison, the effects of non-linearity and viscosity on wave loading and run-up are discussed.     

Experimental Investigation into the performance of the Axial-Flow-Type Waterjet according to the Variation of Impeller Tip Clearance

The tip clearance inside the duct from the tip of the impeller is very important to the performance of waterjet systems, which fact has been proven in the pump field. The tip clearance is especially important on the model scale because it is very difficult in manufacture to keep the tip clearance constant and minimally small along the inside of the duct. In the present study, a flush-type waterjet propulsion unit (duct, impeller, stator, and nozzle) was designed for an amphibious tracked vehicle. Two impellers of different inner diameter were designed and manufactured in order to investigate the gap effect. Resistance and self-propulsion tests with a 1/5-scale model were conducted in PNU towing tank. The flow rate at the nozzle exit, the static pressure at the various sections along the duct and also the nozzle, the revolution of the impeller, and the torque, thrust, and towing forces at various advanced speeds were measured. Based on these measurements, the performance was analyzed according to the ITTC 96 standard analysis method. Based on this analysis method, the full-scale effective and delivered power of the tracked vehicle was estimated according to the variation of tip clearance.     

A NUMERICAL MODEL OF PHYTOPLANKTON BIOMASS IN THE CENTRAL WATERS OF SOUTH CHINA SEA

The present study is based on the materials collected from the middle waters of the South China Sea between 12°-15°N and 111°-118°E during May to July in 1978.The biomass of the phytoplankton (Y, dependent variable) is established to give the biomass of each Species and the environmental factors (i. e. water temperature, salinity, oxygen, silicate and phosphate contents), X1,X2...,X139tt are independent variables that are used to stepwisc regression analysis. Accordicg to the mathematical tests and the practical experience by which a lot of species are deleted and added to the equation, the authors consider that the 4th equation as shown below is optimal one for the model of the biomass on the phytoplankton from this waters.The optimal equation refers to text.     

U37K,δ18O沉积地层记录:南海北部末次盛冰期以来SST和海面变化

应用U37^K和δ^18O重建末次盛冰期以来海水古温度和海平面变化，U37^K-SST沉积记录表现了冰期／间冰期模式．U37^K-SST在冰后期23．3-26．9℃；末次盛冰期22．2-23．8℃．末次盛冰期时古温度比目前平均低4．7℃，南海北部季节性温差冰后期在4．5-7．0℃；末次盛冰期在7．0-9．0℃，远远超过同一纬度太平洋季节温差．末次冰期以来在气候降温的同时，伴有海平面下降，气候的冷暖交替对海平面变化具有决定性的控制意义．     

An initial demonstration of underwater acoustic communication using time reversal

In July 1999, an at-sea experiment to measure the focus of a 3.5-kHz centered time-reversal mirror (TRM) was conducted in three different environments: an absorptive bottom, a reflective bottom, and a sloping bottom. The experiment included a preliminary exploration of using a TRM to generate binary-phase shift keying communication sequences in each of these environments. Broadside communication transmissions were also made, and single-source communications were simulated using the measured-channel response. A comparison of the results is made and time reversal is shown to be an effective approach for mitigating inter-symbol interference caused by channel multipath.     

虾蟹类亲体生殖营养需求研究的进展

本文综述了虾蟹类生殖营养的最新研究成果,并讨论了各营养素对虾蟹类亲体成熟、生殖和幼体质量的影响．此外,就今后在虾蟹类亲体生殖营养方面开展深入研究提出了一些建议。     

An Electronic Chart Display and Information System

This article introduces the most advanced form of electronic chart display and information systems (ECDIS) which complies with the performance standards for ECDIS established by the International Maritime Organization (IMO) and International Hydrographic Organization (IHO). It also describes the key features and the important functions of ECDIS, which we developed. This system is different from the productions developed by C-map, Seven-cs, offshore, and others. It is more suitable for China Marine Safety Administration Bureau to manage the navigation marks real-time. Using this system charts were located and accessed rapidly, and displayed smoothly. This article also discusses in ECDIS application system how to dynamically access and desert charts data in a limited memory (limited computer memory and resource). We constructed a tridimensional structure to manage a large amount of charts data. Vertically, according to the scale range, all charts were divided into eight levels, and a data structure called as “Multiple Scale Quad-tree” was constructed. And horizontally, a grid (every unit has a fixed size) was given to a certain scale level. We call this data structure a “Regular Page.” We explore the implementation of such a structure on the worldwide charts management utilizing quad-trees. Then we discuss the future development of ECDIS and its application system in marine agencies in China.