Cloning and sequence analysis of a full-length cDNA of <Emphasis Type="Italic">SmPP1cb</Emphasis> encoding turbot protein phosphatase 1 beta catalytic subunit

Reversible protein phosphorylation, catalyzed by protein kinases and phosphatases, is an important and versatile mechanism by which eukaryotic cells regulate almost all the signaling processes. Protein phosphatase 1 （PP1） is the first and well-characterized member of the protein serine/threonine phosphatase family. In the present study, a full-length cDNA encoding the beta isoform of the catalytic subunit of protein phosphatase l（PPlcb）, was for the first time isolated and sequenced from the skin tissue of flatfish turbot Scophthalmus maximus, designated SmPPlcb, by the rapid amplification of cDNA ends （RACE） technique. The cDNA sequence of SmPPlcb we obtained contains a 984 bp open reading frame （ORF）, flanked by a complete 39 bp 5＇ untranslated region and 462 bp 3＇ untranslated region. The ORF encodes a putative 327 amino acid protein, and the N-terminal section of this protein is highly acidic, Met-Ala-Glu-Gly-Glu-Leu-Asp-Val-Asp, a common feature for PP1 catalytic subunit but absent in protein phosphatase 2B （PP2B）. And its calculated molecular mass is 37 193 Da and pI 5.8. Sequence analysis indicated that, SmPPlcb is extremely conserved in both amino acid and nucleotide acid levels compared with the PPlcb of other vertebrates and invertebrates, and its Kozak motif contained in the 5＇UTR around ATG start codon is GXXAXXGXXATGG, which is different from mammalian in two positions A6 and G3, indicating the possibility of different initiation of translation in turbot, and also the 3＇UTR of SmPPlcb is highly diverse in the sequence similarity and length compared with other animals, especially zebraf＇lsh. The cloning and sequencing of SmPPlcb gene lays a good foundation for the future work on the biological functions of PP1 in the flatfish turbot.     

Rossby waves with linear topography in barotropic fluids

Rossby waves are the most important waves in the atmosphere and ocean, and are parts of a large-scale system in fluid. The theory and observation show that, they satisfy quasi-geostrophic and quasi-static equilibrium approximations. In this paper, solitary Rossby waves induced by linear topography in barotropic fluids with a shear flow are studied. In order to simplify the problem, the topography is taken as a linear function of latitude variable y, then employing a weakly nonlinear method and a perturbation method, a KdV （Korteweg-de Vries） equation describing evolution of the amplitude of solitary Rossby waves induced by linear topography is derived. The results show that the variation of linear topography can induce the solitary Rossby waves in barotropic fluids with a shear flow, and extend the classical geophysical theory of fluid dynamics.     

面向汉语的自然语言路径描述方法

自然语言路径描述是运用日常生活中人们习惯的语言方式和思维方式来表达路径信息的。而可视化描述和自然语言描述相结合,可以使电子地图向用户提供图文并茂的路径查询服务。目前,电子地图在移动设备和Internet日益普及,自然语言路径描述成为电子地图大众化和人性化服务的重要标志之一。因此,路径描述中需要重点解决空间关系的自然语言描述问题。自然语言中空间关系描述的语言特征与心理学和认知语言学有着非常密切的关系。本文描述了针对汉语的路径描述词汇库的结构与内容,并根据汉语特点设计了路径描述的单元和复合句法模式,以解决词汇受限和句法模式单一的问题,提出了道路名称、沿途标志性地物和方向判断等路径描述补充信息的获取方法,以增加路径描述的可理解性。并以南京市电子地图为例,开发了电子地图查询原型系统。该系统能够在地图上可视化显示最短路径的同时,自动生成该路径的自然语言描述。     

SIG中空间信息可视化与互操作实现研究

在介绍SIG示范验证平台总体架构的基础上,提出了WMS服务实体在该架构下的有效布局、层次结构、服务模型、工作流程等;并介绍了利用开源软件MapServer构建这类WMS服务实体的关键技术.通过WMS服务实体在SIG示范验证平台中的应用实例与效率分析,表明该实现途径与技术是切实可行的.     

数字高程模型对DOM的精度影响

DOM的数字微分纠正通常要用到DEM来实现地形的纠正,DEM的精度会在多大程度上影响DOM的纠正精度,是很多技术人员所关心的问题之一.本文通过详实的论证得出了一些结论,为航空摄影各项技术指标的确定、合理有效地选取DEM数据源等问题作了科学的回答.     

机载LIDAR点云高程数据精度检核及误差来源分析

利用GPSRTK系统对点云高程数据进行质量检核,通过对检核数据的分析,得出实际测量中不同地形情况下LIDAR测高数据能达到的精度,并分析了误差的来源。     

基于MapX的阵列功能的设计与实现

首先讨论了在直角坐标系和地理坐标系下阵列功能的算法原理,详细介绍了基于Delphi和MapX的地理坐标系下阵列功能的设计和算法实现过程。此功能扩展了MapInfo的数字化功能,在一定程度上提高了数字化的效率和精度。     

基于ERMapper软件的QuickBird卫星影像处理实践

采用卫星遥感方法制作的数字正射影像是获取基础空间信息最快速、高效的手段,利用高精度卫星遥感成图,处理速度快、工艺简便、图像清晰,而且精度达到成图要求。本文介绍了以高分辨率的QuickBird卫星影像为研究对象,利用ERMapper图像处理系统进行图像的几何校正、全色与多光谱数据融合方法与过程。     

主成分分析法在油荧光光谱波段选择中的应用

355nm激光器发射激光入射到海水表面,激发海表面溢油的荧光光谱,运用高光谱图像降维中应用广泛的分段主成分分析算法对油荧光光谱进行波段选择。该算法把每个分段被映射到主成分的信息量的大小作为是否被选择的标准,保证了选择波段的信息丰富;通过分段分析消除了传统主成分分析的全局性引起的波段忽略问题,获得较为满意的降维效果。     

滁州市不可移动文物管理信息系统设计和实现

以滁州市为例,在WebGIS技术支持下,通过设计和实现不可移动文物普查与管理数据库、地图基本操作、空间查询分析、文物风险评估以及文物信息输出等功能,改进文物普查与管理工作的方法和手段,提高管理工作的效率和信息化水平,为科研及文物普查管理部门提供辅助决策支持的新思路和新方法.