Diagnosis of iridovirus in large yellow croaker by PCR

A rapid and sensitive PCR-based method for the detection of the large yellow croaker iridovirus(LYCIV) is described,which involves the amplification of a 295 bp fragment of the LYCIV ATPase gene from DNA isolated from naturally infected fish spleen.Sequencing of LYCIV ATPase gene fragment showed it shared 100% nucleotide sequence homology with the corresponding region of the ATPase gene of red sea bream iridovirus(RSIV) and sea bass iridovirus(SBIV),suggesting that LYCIV was homologous with RSIV and SBIV at least in part of the gemone.The specificity and sensitivity of the PCR procedure were tested on the iridovirus-infected fishes,the expected fragment was detected from spleen DNA samples of infected fishes,whereas no fragments were amplified from healthy fish spleen DNA,white spot syndrome baculoviruses(WSBV) DNA and pseudorabies virus(PRV) DNA.Detection limit of this method was 10^-7 ng positive plasmid DNA containing target sequence,equal to about 100 virions.In the infected experiment,first positive detection(1/4) appeared at Day 3 post-infection,all fish(4/4) tested positive at Day 7,however obvious symptoms were observed at Day 8,so LYCIV infection could be detected prior to the appearance of obvious symptoms.These results indicate that this PCR method could be used for early,rapid and specific detection of LYCIV infection.     

水成沉积与风成沉积及古土壤的磁组构特征

晚第四纪水成沉积、风成沉积、古土壤等的磁组构研究表明,不同成因的沉积物具有明显不同的磁组构特征。一般地,水成沉积的体积磁化率Ｋ受物源和水动力因素控制,Ｐ和Ｆ均＞１．０２,ｑ＜０．５;风成沉积的Ｋ受物源控制,内陆黄土的Ｋ明显高于沿海黄土及海岸风沙Ｋ,Ｐ和Ｆ均＜１．０２,ｑ的平均值＞０．５５;古土壤的Ｋ受母质沉积类型和成土环境的氧化还原条件控制,内陆古土壤的Ｋ偏高,沿海古土壤的Ｋ偏低,内陆古土壤的Ｋ远高于沿海古土壤的Ｋ,Ｐ和Ｆ均＜１．０２,ｑ的平均值在０．５左右。运用典型沉积的磁组构特征对比分析未知沉积物,可以得到未知沉积物的物源、沉积过程和环境信息。     

小球藻紫外线诱变及高产藻株筛选

利用单细胞分离技术和紫外诱变育种技术,筛选高产小球藻株。通过单细胞分离技术获得的藻株Chlorella vul-garisQ7,生长速度和蛋白含量均优于其它藻株。以C.vulgarisQ7为出发株,进行紫外诱变,从120株诱变单克隆中筛选出3个株系:M51,M59,M73。与出发株相比,突变株M51,M59,M73的生长速率分别提高了6.23%,3.8%,5.92%。蛋白含量分别提高了2.5%,3.1%,1.9%。研究了诱变株的最适生长条件,结果表明在140μmol.m-2.s-1,25℃时,诱变株具有最大的生长速率和生物量。探讨了诱变株的遗传稳定性,结果表明诱变株可稳定遗传。     

雌雄比对海带雌配子体发育的影响

本实验进行了不同雌雄比对海带雌配子体发育的影响。结果表明：雌雄比(鲜重)为1：1及2：1，实验组出现排卵时间最早，且发育率高，实验第20天，发育率超过70％，孢子体正常。5：1，10：1，20：1实验组随着雄性比例的减少，发育率逐渐降低，随着培养天数的增加，三个实验组均出现未受精卵及畸形孢子体，雌雄比越大，未受精卵数目越多。     

In-Situ Observation of Fluid Mud in the North Passage of Yangtze Estuary, China

Observations of fluid mud were made in the lower North Passage of the Yangtze Estuary in February 2000, on 10 -11 August 2000, on 30 - 31 August 2000 (after two strong typhoons), on 21 - 24 August 2000 (neap tide) and on 3 -6 September 2000 (mean tide) respectively. In situ data show that the fluid mud in this area consists of fine cohesive sediment (median size 7.23 μm). The formation and movement of fluid mud varied during the neap-spring and flood-ebb tidal cycle. Observations suggest that fluid mud phenomena in this area may be categorised in a three-fold manner as slack water, storm and saltwedge features. The thickness of the fluid mud layer of slack water during the neap tide ranged from 0.2 to 0.96 m, whereas during the mean tide, the thickness ranged from 0.17 to 0.73 m, and the thickness of the fluid mud layer was larger during slack water than at the flood peak. Shoals cover an area of 800 km^2 with a water depth smaller than 5 m. Erosion of these extensive intertidal mudflats due to storm action provides an abundant sediment source. This is particularly significant in this estuary when the tidal level is lower than 5 m. The lower North Passage is a typical zone of saltwater wedging, so the saltwedge fluid mud has the most extensive spatial range in the estuary.     

南澎列岛及邻近海域地质地貌与灾害地质分析

南澎列岛及邻近海域是广东沿海灾害地质因素较集中和类型较齐全的地区之一。根据野外调查资料，简要地分析了区内地质地貌以及活动断裂、地震活动、地面沉降、滑坡、地裂和沙土液化等基本特征和分布规律，同时还对区内地质灾害进行了初步分析。     

南沙群岛海区珊瑚礁灾害性地质分析

珊瑚礁是一种特殊的岩土介质，有独特的灾害地质类型。珊瑚礁灾害地质研究是现代工程地质学的一个新课题。通过珊瑚礁的地质记录研究了内动力灾害地质类型（包括活动断裂、地震和火山活动等）、外动力灾害地质类型（包括海底槽谷与地形突变带、崩塌、滑坡、峰礁与埋藏礁、泥丘、埋藏负地貌等），同时还分析了人类活动对珊瑚礁发育的影响。     

Sediment transport pattern at the Barra Nova inlet, south Portugal: a conceptual model

The Barra Nova inlet, in south Portugal, is known to migrate progressively southeastwards under wave action. The morphodynamics of this system during a representative year suggests that this long-term evolution is dependent on a seasonal behavior of the tidal inlet which can be described through a three-stage model of post-storm, transition and extended fair-weather conditions. Processes involved in this evolution indicate that the historical migration of the Barra Nova is not dependent on the longshore drift forcing constricting the channel on the updrift coast, but rather on the adjustment of the system to a major erosion of the downdrift coast during short storm events.     

耳鲍(Haliotis asinina)核糖体小亚基(18S rRNA)编码基因的克隆与序列分析

采用分子生物学的方法,对南海不同海区的两个地理群体耳鲍(Haliotis asinina)18S rRNA基因全长进行了克隆和序列分析,并将耳鲍18S rRNA基因的序列与NCBI数据库中已收录鲍的18SrRNA基因进行了比较。结果发现,南海耳鲍核糖体18S rRNA基因与耳鲍H.asinina isolate H11核糖体18S rRNA基因序列的相同率高达98%;同一地理群体内耳鲍核糖体18S rRNA基因序列完全一致;不同地理群体间耳鲍核糖体18S rRNA基因在碱基组成上的相似率为99%,仅在某些位点处发生了碱基替换,即腺嘌呤(T)被鸟嘌呤(G)替换;同时,将这两个不同群体中耳鲍的18S rRNA基因与泰国耳鲍18S rRNA基因序列进行比较分析发现,它们之间也只是发生了碱基替换。     

深圳大鹏湾表层沉积物碎屑矿物空间分布特征

立足样品测试数据,利用空间分析、空间插值、地质统计以及景观生态学方法,深入分析了深圳大鹏湾表层沉积物碎屑矿物的空间分布特征,并使用球状模型对相应半变异函数进行理论建模。结果表明,碎屑矿物分布的Hurst分维数为0.28～0.97,空间几何分维数为1.03～1.72;Moran Ⅰ型空间自相关系数-0.15～0.53。在此基础上,以等值线分布所包围范围作为划分尺度,以Shannon-Wiener信息指数作为度量,得到的矿物分布所反映出的信息量或者富集程度指数为0.88～1.85。