两株琼胶酶高产细菌的筛选和鉴定

从海洋环境中筛选得到两株琼胶酶的高产菌株，通过形态观察和生理生化反应，确定它们属于弧菌属，通过BIOLOG细菌鉴定系统鉴定，并同弧菌属标准菌株分析比较，确定上方宝剑两株菌都是塔式弧菌（Vibrio tubiashii)，这两株菌在碳源利用方面存在差别。     

A field-based population model for the sediment toxicity test organism Leptocheirus plumulosus: I. Model development

We describe the development of a stage-structured population matrix model for the estuarine amphipod Leptocheirus plumulosus, a sediment toxicity test organism, based on regular sampling of a natural population for approximately 2 years. Annual population dynamics were characterized by abundance peaks in the spring and fall, and low densities in the summer and winter. We use a sequence of seasonal projection matrices to capture these qualitative patterns in population dynamics. We compare two methods for estimating transition probabilities: multiple regression and truncated singular value decomposition (SVD). Overall, SVD gave more realistic parameter estimates than regression, although fecundity estimation was problematic. There were predictable trends in growth, survival and fecundity within years. The SVD-derived model provides a framework for assessing the population-level effects of contaminants on Leptocheirus.     

Short-term and interannual variability of redox-sensitive chemical parameters in hypoxic/anoxic bottom waters of the Chesapeake Bay

A combination of CTD casts, discrete bottle sampling and in situ voltammetric microelectrode profiling was used to examine changing redox conditions in the water column at a single station south of the Bay Bridge in the upper Chesapeake Bay in late July/early August, 2002–2005. Short-term (2–4 h) fluctuations in the oxic/suboxic/anoxic interface were documented using in situ voltammetric solid-state electrodes. Profiles of dissolved oxygen and sulfide revealed tidally-driven vertical fluctuations of several meters in the depth and thickness of the suboxic zone. Bottom water concentrations of sulfide, Mn²⁺ and Fe²⁺ also varied over the tidal cycle by approximately an order of magnitude. These data indicate that redox species concentrations at this site varied more due to physical processes than biogeochemical processes. Based on analysis of ADCP data, tidal currents at this station were strongly polarized, with the principal axis of tidal currents aligned with the mainstem channel. Together with the chemical data, the ADCP analysis suggests tidal flushing of anoxic bottom waters with suboxic water from north of the site. The present study is thus unique because while most previous studies have focused on processes across relatively stable redox interfaces, our data clearly demonstrate the influence of rapidly changing physical mixing processes on water column redox chemistry.Also noted during the study were interannual differences in maximum bottom water concentrations of sulfide, Mn²⁺ and Fe²⁺. In 2003, for example, heavy spring rains resulted in severe hypoxia/anoxia in June and early July. While reported storm-induced mixing in late July/early August 2003 partially alleviated the low-oxygen conditions, bottom water concentrations of sulfide, Mn²⁺ and Fe²⁺ were still much higher than in the previous year. The latter implies that the response time of the microbial community inhabiting the suboxic/anoxic bottom waters to changing redox conditions is slow compared to the time scale of episodic mixing events. Bottom water concentrations of the redox-sensitive chemical species should thus be useful as a tracer to infer prior hypoxic/anoxic conditions not apparent from ambient oxygen levels at the time of sampling.     

Assessment of spatial variation in carbon utilization by benthic and pelagic invertebrates in a temperate South African estuary using stable isotope signatures

Stable isotope analyses (δ¹³C and δ¹⁵N) were used to evaluate the spatial variations in carbon flow from primary producers to consumers at two sites in the temperate and permanently open Kariega Estuary on the southeastern coast of South Africa during October 2005 and February 2006. One site was located opposite a salt marsh while the second was upstream of the marsh. Except for significantly enriched δ¹³C values of Zostera capensis and surface sediments near the salt marsh, the δ¹³C and δ¹⁵N signatures of the producers were similar between sites. The invertebrates were clustered into groups roughly corresponding to the predominant feeding modes. The suspension feeders showed δ¹³C values closest to the seston, whereas the deposit feeders, detritivores and scavengers/predators had more enriched δ¹³C values reflecting primary carbon sources that were likely a combination of seston, Spartina maritima and Z. capensis at the upstream site, with an increased influence of benthic algae and Z. capensis at the salt marsh site. The δ¹⁵N signatures of the consumers showed a stepwise continuum rather than distinct levels of fractionation, indicating highly complex trophic linkages and significant dietary overlap among the species. Consumers exhibited significantly enriched δ¹³C values at the salt marsh site, an effect that was attributed to enriched Z. capensis detritus in this region in addition to increased phytoplankton biomass in their diets compared with invertebrates living upstream. The data reinforce the concept that between-site variations in the stable isotope ratios of consumers can result not only from dietary shifts, but also from alterations in the isotope ratios of primary producers.     

Recruitment patterns of Serpula vermicularis L. (Polychaeta, Serpulidae) in Loch Creran, Scotland

This study aimed to contribute to conservation management of reefs of Serpula vermicularis by increasing understanding of the factors influencing larval settlement. The study was carried out in Loch Creran, which supports the most extensive known development of S. vermicularis reefs in the world. Settlement plates were deployed to examine the influence of season, depth, reef density, substrate type and orientation. Monthly deployment of plates revealed settlement of S. vermicularis to occur predominantly from mid-June to mid-October, peaking in late August to early September. Settlement of Pomatoceros spp. peaked much earlier, in late May to early June. Deployment of plates at different depths revealed a marked reduction in S. vermicularis settlement intensity between 6 and 12 m. As this corresponds with the deeper limit of the peripheral fringe of serpulid reefs in the loch, it is suggested that this limit is imposed by a depth-correlated settlement response, rather than reduction in available substrata. Comparisons of various substrata showed a preference by S. vermicularis larvae for a slate over a scallop substrate and no evidence of enhanced recruitment to occupied or unoccupied tubes of S. vermicularis, suggesting that gregarious attraction is unlikely to be a factor causing reef formation. Settlement onto the upper side of a horizontal scallop substrate was found to be insignificant in comparison with the underside or a vertically orientated scallop. Evidence for the role of light in controlling the depth and substrate-orientation preferences of S. vermicularis larvae is discussed. Based on the results of this study, recommendations are made regarding remediation of areas suffering reef damage.     

Manifestation of the Arctic Oscillation and El Niño-Southern Oscillation in the tropical stratosphere

Characteristics of the Arctic Oscillation and El Niño-Southern Oscillation effects manifested on interannual scales in the equatorial stratosphere are determined. Wavelet analysis of local phase shifts, coherence, and correlation is used to obtain correlation portraits of the largest factors of climate variability against the background of coherent variations in the equatorial stratospheric wind speed at the 50- and 15-hPa pressure levels. It is shown that the Arctic Oscillation and El Niño-Southern Oscillation signals may reach the tropical stratosphere. The signals are easily identified in a wide range of scales, including quasi-biennial, 3-to 5-year, and 10-to 11-year periods. The results obtained reflect a coherent pattern of the manifestation of these signals at the selected stratospheric levels. It is found that the El Niño-Southern Oscillation effect at periods close to 10–11 years reaches the stratospheric level rather rapidly, in the same or next month, while the effects of the Arctic Oscillation index are delayed by nine months. The estimates obtained show that a phase shift of almost 180° in the Arctic Oscillation index relative to the equatorial stratospheric wind occurred in almost all of the range of interannual periods in 1978 and 1992. For the El Niño-Southern Oscillation, an increase in local correlations on a scale of 3-to 5-year variations was observed in 1980–1990, a 180° phase shift occurred in 1992, and the correlation with stratospheric wind increased in 1992–2004. The estimates obtained are indicative of a change in the atmospheric circulation pattern that took place in the Northern Hemisphere in 1978–1991.     

Geological structure of the Curonian Spit (of the Baltic Sea) and its evolution history (revised)

The origin and evolution history of the Curonian Spit in the Baltic Sea are discussed based on geological and geomorphologic data. Evidence of the correlation between the spit formation stages and the Holocene sea level oscillations is presented. The ledges of moraine basement and marine accumulative features formed a single barrier. Its subsequent transformation occurred under the influence of the wave-related lithodynamic and eolian processes.     

The Pechora Sea: Past,recent, and future

The main stages in the development of the Pechora Sea are discussed. It is established that, during the high sea level stand corresponding to the warmest epoch of the Mikulino Interglacial, the Pechora Sea represented a more spacious, as compared with its present-day size, basin owing to the flooded valleys of river lower reaches. No sea in its present-day configuration existed during the last (Valdai) glaciation. At that time, the sea could have occupied only a narrow area along the southern coast of Novaya Zemlya, where marine sedimentation was in progress during the Late Pleistocene and Holocene. During the glaciation and postglacial time, the dried bottom of the former Pechora Sea accumulated large volumes of sand that are now concentrated largely in the accretion structures along its southern coast. In the current century, changes will occur mainly in the coastal zone of the Russkii Zavorot Peninsula, Pesyakov Island, the Varandei Settlement area, and the Medynskii Zavorot Peninsula, where a shoreline retreat for a distance of 0.5 km is expected.     

Supply and precipitation of hydrothermal iron in the rift valley of the Mid-Atlantic Ridge at 26° and 29° N

This publication considers the probing data on aquatic anomalies (hydrothermal plumes) in the areas of 26° and 29° N of the Mid-Atlantic Ridge (MAR). The mass of the hydrothermal iron supply and the intensity of the iron sedimentation onto the bottom were estimated by means of sediment traps. It was found that the plume of the TAG hydrothermal vent 6 km³ in volume contained about 67 t of suspended Fe; the plume of the Broken Spur field (up to 8.24 km³ in volume) contained 23.5 t or less because of the lower concentration. The data on the sedimentary matter fluxes showed that 0.3–0.5% of the hydrothermal iron was precipitated immediately from a plume of neutral buoyancy onto the bottom; the bulk of the iron was dissipated into the environment. From the dimensions of the plumes, the flow dynamics, the iron concentrations in the plumes, and the amounts of iron supplied by hydrothermal vents, it was found that the resident time of the plumes considered was from 5 to 10 days.