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151.
Filamentous Bangia, which are distributed extensively throughout the world, have simple and similar morphological characteristics. Scientists can classify these organisms using molecular markers in combination with morphology. We successfully sequenced the complete nuclear ribosomal DNA, approximately 13 kb in length, from a marine Bangia population. We further analyzed the small subunit ribosomal DNA gene (nrSSU) and the internal transcribed spacer (ITS) sequence regions along with nine other marine, and two freshwater Bangia samples from China. Pairwise distances of the nrSSU and 5.8S ribosomal DNA gene sequences show the marine samples grouping together with low divergences (00.003; 0–0.006, respectively) from each other, but high divergences (0.123–0.126; 0.198, respectively) from freshwater samples. An exception is the marine sample collected from Weihai, which shows high divergence from both other marine samples (0.063–0.065; 0.129, respectively) and the freshwater samples (0.097; 0.120, respectively). A maximum likelihood phylogenetic tree based on a combined SSU-ITS dataset with maximum likelihood method shows the samples divided into three clades, with the two marine sample clades containing Bangia spp. from North America, Europe, Asia, and Australia; and one freshwater clade, containing Bangia atropurpurea from North America and China. 相似文献
152.
The culture of Sertoli cells has become an indispensable resource in studying spermatogenesis. A new Sertoli cell line (POSC) that consisted predominantly of fibroblast-like cells was derived from the testis of the olive flounder Paralichthys olivaceus and sub-cultured for 48 passages. Analysis of the mtDNA COI gene partial sequence confirmed that the cell line was from P. olivaceus. Cells were optimally maintained at 25°C in DMEM/F12 medium supplemented with fetal bovine serum, basic fibroblast growth factor, and epidermal growth factor. The growth curve of POSC showed a typical “S” shape. Chromosome analysis revealed that the cell line possessed the normal P. olivaceus diploid karyotype of 2n=48t. POSC expressed dmrt1 but not vasa, which was detected using RT-PCR and sequencing. Immunocytochemistry revealed that the cells exhibited the testicular Sertoli cell marker FasL. Therefore, POSC appeared to consist of testicular Sertoli cells. Bright fluorescent signals were observed after the cells were transfected with pEGFP-N3 plasmid, with the transfection efficiency reaching 10%. This research not only offers an ideal model for further gene expression and regulation studies on P. olivaceus, but also serves as valuable material in studying fish spermatogenesis, Sertoli cell-germ cell interactions, and the mechanism of growth and development of testis. 相似文献
153.
Herbaceous plants are widely distributed on islands and where they exhibit spatial heterogeneity. Accurately identifying the impact factors that drive spatial heterogeneity can reveal typical island biodiversity patterns. Five southern islands in the Miaodao Archipelago, North China were studied herein. The spatial distribution of herbaceous plant diversity on these islands was analyzed, and the impact factors and their degree of impact on spatial heterogeneity were identified using CCA ordination and ANOVA. The results reveal 114 herbaceous plant species, belonging to 94 genera from 34 families in the 50 plots sampled. The total species numbers on different islands were significantly positively correlated with island area, and the average α diversity was correlated with human activities, while the β diversity among islands was more affected by island area than mutual distances. Spatial heterogeneity within islands indicated that the diversities were generally high in areas with higher altitude, slope, total nitrogen, total carbon, and canopy density, and lower moisture content, pH, total phosphorus, total potassium, and aspect. Among the environmental factors, pH, canopy density, total K, total P, moisture content, altitude, and slope had significant gross effects, but only canopy density exhibited a significant net effect. Terrain affected diversity by restricting plantation, plantation in turn influenced soil properties and the two together affected diversity. Therefore, plantation was ultimately the fundamental driving factor for spatial heterogeneity in herbaceous plant diversity on the five islands. 相似文献
154.
ZHENG Tingting BAI Yi WANG Qiang ZHU Xufeng FANG Kaiyong YAO Yuan ZHAO Yongqiang WANG Xiaolin 《《地质学报》英文版》2018,92(3):897-903
A new ootype collected from the Upper Cretaceous Lijiacun Formation in the Shangdan Basin, Shaanxi Province is described in this paper. Based on general external shape, size, eggshell thickness and honeycomb‐like eggshell microstructure, eggs are referable to the oofamily Faveoloolithidae. Compared with other members of Faveoloolithidae, specimens described in this paper show special characteristics: adjacent pores are usually separated by two eggshell units between which often develop interspaces; columnar eggshell units are relatively closely arranged in radial view. According to these characteristics, we erect a new oogenus and a new oospecies: Duovallumoolithus shangdanensis oogen. et oosp. nov. The new discovery expands the diversity of Faveoloolithidae. 相似文献
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位于贵州铜仁松桃国家锰矿整装勘查区内的高地富锰矿床,(332)+(333)类碳酸锰富锰矿石资源量达7 166. 84万吨,锰平均品位25. 75%,矿体平均厚度4. 52 m,富锰矿体赋存于总资源量达1. 61亿吨的高地超大型锰矿床之中,由一个单一的富锰矿体构成,是我国新发现的首个特大型富锰矿床,富锰矿资源量相当于我国富锰矿资源量总和的2倍。成因类型属古天然气渗漏沉积型锰矿床,富锰矿产于古天然气渗漏沉积成矿系统中心相区。高地富锰矿床地处独特的"新月形"南华纪早期李家湾-高地-道坨(Ⅳ级)地堑盆地中心,菱锰矿体中均出现以块状构造夹被沥青充填的气泡状富菱锰矿石。地表燕山期北北东向的断裂构造十分发育,但主要为上陡下缓犁式正断层,未触及高地深部隐伏巨型锰矿体,以致富锰矿体保存亦十分完整,易于开发利用。 相似文献
160.
Qiuping Huang Jiejun Huang Yunjun Zhan Wei Cui Yanbin Yuan 《Earth Science Informatics》2018,11(4):567-578
Quantifying land use patterns and functions is critical for modeling urban ecological processes, and an emerging challenge is to apply models at multiple spatial scales. Methods of determining the optimum scale of land use patterns are commonly considered using landscape metrics. Landscape metrics are quantitative indicators for analyzing landscape heterogeneity at the landscape level. In this study, due to their widespread use in urban landscape analyses and well-documented effectiveness in quantifying landscape patterns, landscape metrics that represent dominance, shape, fragmentation and connectivity were selected. Five metrics include Patch Density, Contagion, Landscape Shape Index, Aggregation Index and Connectivity. Despite a wide application of landscape metrics for land use studies, the majority mainly focuses on the qualitative analysis of the characteristics of landscape metrics. The previous models are limited in exploring the optimum scale of land use patterns for their lack of quantitation. Therefore, taking the City of Wuhan as an example, the land use unit was treated as a patch, and the landscape pattern metrics at different spatial scales were calculated and compared so as to find the optimum one. Furthermore, a mathematical model of landscape metrics was proposed to quantify the scale effect of urban land use patterns, generating a complementary tool to select the optimum scale. In addition, Analytic Hierarchy Process (AHP) was introduced to determine the respective weights of the chosen landscape metrics in this model. Fractal dimension was ultimately applied to verify the chosen optimum scale of our study region. The results indicated that 60 m is confirmed to be the optimum scale for capturing the spatial variability of land use patterns in this study area. 相似文献