造礁石珊瑚共生藻的分子分类研究

通过nlsrDNA(nuclear large-subunit ribosomal DNA)及nssrDNA(nuclear small-subunit ribosomal DNA)的PCR-RFLP研究广东徐闻地区8科15属25种62个造礁石珊瑚样本的共生藻。结果表明,共生藻nlsrDNA的RsaI酶切基因型只存在一种,属于C系群共生藻;而nssrDNA的MobⅠ和TaqⅠ两种酶切都存在两种基因型。实验进一步通过PCR直接测序法得到62个造礁石珊瑚样品的共生藻ITS序列,与GenBank上的4种虫黄藻ITS序列构建Neighbor-Joining系统发育树,结果表明该区的造礁石珊瑚共生两种不同种类(亚系群)的共生藻,分别为C1亚系群与C15亚系群共生藻,两个亚系群间的遗传距离为0.019。广东徐闻地区造礁石珊瑚共生藻多样性偏低,暗示该地区珊瑚礁生态系统应对环境变化的能力可能较弱。     

Photocatalytic degradation of spill oils on TiO2 nanotube thin films

The nitrogen-doped TiO₂ nanotube (N-TNT) thin films were synthesized using ZnO nanorods as the template and doped with urea at 623 K. Under ultraviolet (UV) and visible light irradiation, the efficiencies for photocatalytic degradation of methylene blue is as high as 30%. About 10% of toluene (representing aromatics in the spill oils) in sea water can be photocatalytically degraded under visible light radiation for 120 min. The aliphatic model compound (1-hexadecene) has, on the contrary, a less efficiency (8%) on the N-TNT photocatalyst. On the average, under visible light radiation, the effectnesses of the N-TNT for photocatalytic degradation of model compounds in the spill oils in sea water are 0.38 mg toluene/gN-TNT h and 0.25 mg 1-hexadecene/gN-TNT h. It is expected that spill oils in the harbors or seashores can be adsorbed and photocatalytically degraded on the N-TNT thin films that are coated onto levee at the sea water surface level.     

Using ADV backscatter strength for measuring suspended cohesive sediment concentration

Laboratory experiments were conducted at two institutes to reveal the relationship between acoustic backscatter strength and suspended sediment concentration (SSC). In total, three acoustic Doppler velocimeters (ADVs) with different frequencies (5, 10 and 16 MHz) were tested. Two different commercial clays and one natural sediment from Clay Bank site in the York River were checked for acoustic responses. The SSCs of selected sediments were artificially changed between a selected low and a high value in tap or de-ion water. Each ADV showed quite different backscatter responses depending on the sediment type and SSC. Not all devices had a good linear relationship between backscatter strength and SSC. Within a limited range of SSC, however, the backscatter strength can be well correlated with the SSC. Compared with optical backscattering sensor (OBS), the fluctuation of ADV backscatter signals was too noisy to be directly converted to the instantaneous changes of SSC due to high amplification ratio and small sampling volume. For the more accurate signal conversion for finding the fluctuation of SSC, the ensemble average should be applied to increase the signal-to-noise ratio. There are unexpected responses for the averaged backscatter wave strength: (1) high signals from small particles but low signals from large particles; and (2) two linear segments in calibration slope. These phenomena would be most likely caused by the different gain setting built in ADVs. The different acoustic responses to flocculation might also contribute somewhat if flocs are tightly packed. This study suggests that an ADV could be a useful instrument to estimate suspended cohesive sediment concentration and its fluctuation if the above concerns are clarified.     

Core-log integration studies in hole-A of Taiwan Chelungpu-fault Drilling Project

Taiwan Chelungpu-fault Drilling Project (TCDP) was initiated to understand the physical mechanisms involved in the large displacements of the 1999 Taiwan Chi-Chi earthquake. Continuous measurements of cores (including laboratory work) and a suite of geophysical downhole logs, including P - and S -wave sonic velocity, gamma ray, electrical resistivity, density, temperature, electrical borehole images and dipole-shear sonic imager, were acquired in Hole-A over the depth of 500–2003 m. Integrated studies of cores and logs facilitate qualitative and quantitative comparison of subsurface structures and physical properties of rocks. A total of 10 subunits were divided on the basis of geophysical characteristics. Generally, formation velocity and temperature increase with depth as a result of the overburden and thermal gradient, respectively. Gamma ray, resistivity, formation density, shear velocity anisotropy and density-derived porosity are primarily dependent on the lithology. Zones with changes of percentage of shear wave anisotropy and the fast shear polarization azimuth deduced from Dipole Shear-Imager (DSI) are associated with the appearance of fractures, steep bedding and shear zones. The fast shear wave azimuth is in good agreement with overall dip of the bedding (approximately 30° towards SE) and maximum horizontal compressional direction, particularly in the Kueichulin Formation showing strong shear wave velocity anisotropy. Bedding-parallel fractures are prevalent within cores, whereas minor sets of high-angle, NNW–SSE trending with N- and S-dipping fractures are sporadically distributed. The fault zone at depth 1111 m (FZA1111) is the Chi-Chi earthquake slip zone and could be a fluid conduit after the earthquake. The drastic change in fast shear wave polarization direction across the underlying, non-active Sanyi thrust at depth 1710 m reflects changes in stratigraphy, physical properties and structural geometry.     

Modelling of hydrodynamics and cohesive sediment transport in Tanshui River estuarine system,Taiwan

A laterally averaged two-dimensional numerical model is used to simulate hydrodynamics and cohesive sediment transport in the Tanshui River estuarine system. The model handles tributaries as well as the main stem of the estuarine system. Observed time series of salinity data and tidally averaged salinity distributions have been compared with model results to calibrate the turbulent diffusion coefficients. The overall model verification is achieved with comparisons of residual currents and salinity distribution. The model reproduces the prototype water surface elevation, currents and salinity distributions. Comparisons of the suspended cohesive sediment concentrations calculated by the numerical model and the field data at various stations show good agreement. The validated model is applied to investigate the tidally averaged salinity distributions, residual circulation and suspended sediment concentration under low flow conditions in the Tanshui River estuarine system. The model results show that the limit of salt intrusion in the mainstem estuary is located at Hsin-Hai bridge in Tahan Stream, 26 km from the River mouth under Q75 flow. The null point is located at the head of salt intrusion, using 1 ppt isohaline as an indicator. The tidally averaged sediment concentration distribution exhibits a local maximum around the null point.     

Design of a high performance variable structure position control ofROVs

An adaptive control scheme for dynamic positioning (DP) of remotely operated underwater vehicles (ROV) is proposed based on a recently developed output feedback variable structure control (VSC) algorithm named VS-MRAC. Only position measurement is required. Precise modeling of the ROV is not needed and unmodeled perturbations can be effectively rejected. A simple method for discretizing the original continuous-time VS-MRAC is proposed based on dead-beat response. Other important practical implementation issues are considered. The performance is evaluated by simulation with a realistic ROV model and by full-scale experimental pool tests with an actual ROV     

Locational Decisions and Residential Preferences of Taiwanese Immigrants in Australia

Since the 1980s, the increase of Asian migrants in large cities has contributed significantly to the cultural landscape of Australia, especially in the city suburbs. Based on the Census and in-depth interviews of 103 Taiwanese migrants, this research examines the location decisions and residential preferences of Taiwanese migrants in Sydney, Brisbane and Melbourne. The reasons for choosing the state, the particular city, and specific area of residence are probed into. The survey shows that migrants moved to Australia mainly for children’s education, lifestyle and political instability reasons. The main reasons for choosing to settle in Sydney were favourable business opportunities, and the northern part of Sydney was preferred. Those who chose Brisbane are attracted by the good climate there, and the residents preferred the Sunnybank area. Melbourne was chosen for the good education it offers, and areas on transportation lines near good schools are selected. Choice of residence was based mainly on their needs and their resources which may change over time. In general, they always choose to locate in close proximity to their family, friends and Taiwanese communities. Their locational choice depends on income and housing budget, proximity to good schools and shopping centers, and information from friends or earlier migrants. Based on such factors, this study clearly demonstrates that the Taiwanese immigrants are fundamentally different from early Chinese settlers who once aimed to settle at Chinatown locations.     

Present state and historical changes of trace metal pollution in Kaoping coastal sediments, southwestern Taiwan

Surface and gravity-cored sediments were collected from the Kaoping coastal area off southwestern Taiwan to determine particle size, organic carbon, trace metal concentration and enrichment factor (EF), 210Pb dating, and 206Pb/207Pb ratio for determining present and historical metal pollution. Surface distributions of trace metals ranged from 0.02 to 0.13 mg/kg for Cd (EF: 0.16-1.65), from 35 to 189 mg/kg for Cr (EF: 0.85-2.92), from 25 to 64 mg/kg for Ni (EF: 0.53-2.38), from 10 to 32 mg/kg for Pb (EF: 1.20-4.94), and from 29 to 129 mg/kg for Zn (EF: 1.18-3.50). Trace metal concentrations correlate closely with distributions of mud (<63 microm) and organic carbon which accumulate largely around river mouths and within the Kaoping Canyon. With the exception of Cd, Cr and Ni in certain areas with rather coarse sediments, metals were generally elevated above the baseline levels over the studied area. Metals were also relatively enriched in areas with high contents of mud and organic carbon. Sedimentation rates derived from the excess 210Pb data in core sediments sampled from the canyon illustrate metal pollution beginning around 1970 that is corresponding to the booming time of economic growth in Taiwan. Meanwhile, the status of Pb pollution in core sediments is verified by an inversed correlation between 206Pb/207Pb ratios and Pb concentrations. Anthropogenic Pb and other metals in the studied area were apparently derived primarily from the Kaoping River and accumulated around the river mouth and within the Kaoping Canyon. The Kaoping Canyon appears to act as a major sink for river borne trace metals.     

New evidence for covalent coupling of peptides to humic acids based on 2D NMR spectroscopy: A means for preservation

Nitrogen immobilization in soils and sediments involving the preservation of peptides is an important yet not well-understood process. Several hypotheses have been proposed for the preservation of peptides in these systems; however, to date, there is no direct molecular-level evidence for any one of several hypotheses. In this study, a synthesized peptide with ¹⁵N-labeled amino acid residues was utilized to examine the mechanism by which humic substances from a peat might chemically interact with peptides to induce preservation. Two-dimensional ¹⁵N-¹H HSQC NMR experiments were performed to evaluate the bonding and nonbonding interactions between ¹⁵N-labeled peptide and an Everglades peat humic acid. The observed changes in cross peaks provide the first direct spectroscopic evidence for what we believe to be covalent binding between these substances. Noncovalent interactions between the ¹⁵N-labeled peptides and humic acid molecules are also observed in the spectra. Quinone structures in humic acids are suggested to be the important reactive groups, based on reaction of the labeled peptide with a model quinone which shows similar nitrogen-proton correlated peak patterns as is observed in the HSQC NMR spectra of humic acids reacted with the peptide. The Michael reaction with quinone structures is proposed, and this type of reaction provides a mechanism that is consistent with previous observation for the chemistry of sedimentary nitrogen species.     

Establishing the Database of Inundation Potential in Taiwan

The structural measure was the major solution for flood defense in Taiwan. However, the measure is always limited to the design standard and cannot prevent the damages when floods exceed certain scale. Therefore, non-structural measures for flood mitigation are the indispensable complements to structural solutions. The study introduces the establishment of inundation potential database that provides required information for the non-structural measures in Taiwan. The database was built by numerical simulations, based on different rainfall scenarios, and has been applied by the local governments of Taiwan for land use managements, flood warning systems, emergency responses, and flood insurance programs to reduce the flood damages and impacts.