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An open reading frame (lcn61) of lymphocystis disease virus China (LCDV-cn), probably responsible for encoding putative zinc-finger proteins was amplified and inserted into pET24a (+) vector. Then it expressed in E. coli BL21 (DE3), and His-tag fusion protein of high yield was obtained. It was found that the fusion protein existed in E. coli mainly as inclusion bodies. The bioinformatics analysis indicates that LCN61 is C2H2 type zinc-finger protein containing four C2H2 zinc-finger motifs. This work provides a theory for functional research of lcn61 gene. Supported by High Technology Research and Development Program of China (863 Program, No. 2006AA100309)  相似文献   
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Based on a ship survey during January 1998, the characteristics of the flow, the thermohaline properties and the volume transport of the Arabian Sea are discussed. A strong westward flow exists between 10.5?N and 11?N, part of which turns to the south as the Somali current near the coast at about 10?N and the rest turns north. At the passage between the African continent and the So- cotra Island, the northern branch separates into two flows: the left one enters the passage and the right one flows eastward along the southern slope of the island. Off the island the flow separates once more, most of it meandering northeast and a small fraction flow- ing southeast. Volume transport calculation suggests that the tidal transport is one or two orders of magnitude smaller than the total transport in this region and it becomes more important near the coast. The average velocity of the flow in the upper layer (0-150 m) is about 20 cm s-1, with a maximum of 53 cm s-1 appearing east of the Socotra Island, and the subsurface layer (200-800 m) has an aver- age velocity of 8.6 cm s-1; the velocity becomes smaller at greater depths. The depth of the seasonal thermocline is about 100 m, above which there is a layer with well mixed temperature and dissolved oxygen. High-salinity and oxygen-rich water appears near the surface of the northern Arabian Sea; a salinity maximum and oxygen minimum at 100 m depth along 8?N testifies the subduction of surface water from the northern Arabian Sea. Waters from the Red Sea and the Persian Gulf also influence the salinity of the area.  相似文献   
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1IN T R O D U C T IO NInteractionsbetween the ocean and atmosphere con-tributeto climatefluctuationosver a broad spectrum oftime scales.Studiesof those interactionhsave farfo-cused on El Ni o-SouthernOscillation(ENSO)phe-nomenon thathas a period of3to4yea…  相似文献   
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We report our findings on a new quasi-periodic oscillation (QPO) and a long period from the ultraluminous X-ray source (ULX) X-2 in nearby galaxy NGC 4736 based on the Chandra and XMM-Newton archival data. To examine the timing properties, power density spectra of the source have been obtained using Fast Fourier Transform. Also the spectral parameters of the source have been calculated by obtaining and fitting the energy spectra. Power density spectrum of this source reveals a QPO peak at \(0.73_{-0.14}^{+0.16}~\mathrm{mHz}\) with an fractional rms variability of 16 % using the Chandra data (in the year 2000-lower state of the source). The XMM-Newton data analysis indicates a peak at \(0.53_{-0.35}^{+0.09}~\mathrm{mHz}\) with a fractional rms variation of 5 % (in the year 2006-higher state of the source). These recovered QPOs overlap within errors and may be the same oscillation. In addition, we detect a long periodicity or a QPO in the Chandra data of about (5.2±2.0)×10?5 Hz (~5.4 h) over 3 σ confidence level. If this is a QPO, it is the lowest QPO detected from a ULX. The mass of the compact object in ULX X-2 is estimated using the Eddington luminosity and a disk blackbody model in the range (10–80) M .  相似文献   
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Mega-urban agglomerations are strategic core areas for national economic development and the main regions of new urbanization. They also have important roles in shifting the global economic center of gravity to China. However, the development of mega-urban agglomerations has triggered the interactive coercion between resources and the eco-environment. The interactive coupled effects between urbanization and the eco-environment in mega-urban agglomerations represent frontier and high-priority research topics in the field of Earth system science over the next decade. In this paper, we carried out systematic theoretical analysis of the interactive coupling mechanisms and coercing effects between urbanization and the eco-environment in mega-urban agglomerations. In detail, we analyzed the nonlinear-coupled relationships and the coupling characteristics between natural and human elements in mega-urban agglomerations. We also investigated the interactive coercion intensities between internal and external elements, and the mechanisms and patterns of local couplings and telecouplings in mega-urban agglomeration systems, which are affected by key internal and external control elements. In addition, we proposed the interactive coupling theory on urbanization and the eco-environment in mega-urban agglomerations. Furthermore, we established a spatiotemporal dynamic coupling model with multi-element, multi-scale, multi-scenario, multi-module and multi-agent integrations, which can be used to develop an intelligent decision support system for sustainable development of mega-urban agglomerations. In general, our research may provide theoretical guidance and method support to solve problems related to mega-urban agglomerations and maintain their sustainable development.  相似文献   
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本文介绍新滩滑坡后两岸边坡监测的工作,对边坡变形提出了趋势意见。认为新滩斜坡目前正处于整体稳定下的局部调整阶段,变形缓慢平稳,但要注视广家崖的危岩动态;链子崖仍有趋势性的倾江形变。5—6号缝围成的7万方危岩及江段的5万方危岩体尚有一触即崩之势,须加强监视。  相似文献   
19.
中国“四纵四横”高铁网络可达性综合评估与对比   总被引:7,自引:1,他引:7  
传统测算可达性的方法较为单一,缺乏多层面、多角度的综合研究和对比分析。基于时间、经济与重心视角利用可达性模型并结合ArcGIS 空间分析手段探究高铁通车前后沿线城市可达性的动态变化;利用综合变异系数、层级分析法和改进的哈夫模型分析高铁可达性空间演变特征及规律,构建高铁可达性评价体系并结合熵权法定量评估各高铁可达综合实力。研究表明:京广、京沪可达综合实力最强,沪昆、杭福深次之,沪汉蓉、哈大、青太再次之,郑西、兰新可达实力最弱;全国高铁可达性强弱变化呈明显地带性规律,东、中部高铁可达性强于东北,东北强于西部,纵向高铁强于横向;经济潜力的增加率明显高于加权平均旅行时间的减少率,可达性重心偏移驱使不同等时圈蔓延交叠;高铁以“核心—核心”逐步向“核心—网络”空间链接模式过渡,产生上海、北京、广州、深圳4 个高铁“国家服务中心”,天津、武汉、重庆等6 个“大区域服务中心”,形成“多中心”高铁服务格局以及日益庞大而复杂的高铁特质空间集群;高铁网络影响下的中国区域空间格局的渐变与重塑日趋复杂,“T”型轴带呈现出由空间极化向空间均衡转变,跨城流动性特征突显,加速空间对接与同城化进程。  相似文献   
20.
IntroductionIn color reproduction and communication, met-amer exists inherently when the conventionalcondition matching used. Therefore , the colordifference introduced makes the accuracy of colorrepresentation be lowered.In order to eli minatemetamer CIE developed the scheme so-calledspectra matching in Publication 15 .2 and speci-fied that using band-pass filters with 5 nminter-val to sample and then reconstruct the originalcolor signal ,thus the color difference will disap-pear[1].Spectra…  相似文献   
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