Identification and bioinformatics analysis of microRNAs from the sporophyte and gametophyte of <Emphasis Type="Italic">Pyropia haitanensis</Emphasis>

Pyropia haitanensis (T. J. Chang et B. F. Zheng) N. Kikuchi et M. Miyata (Porphyra haitanensis) is an economically important genus that is cultured widely in China. P. haitanensis is cultured on a larger scale than Pyropia yezoensis, making up an important part of the total production of cultivated Pyropia in China. However, the majority of molecular mechanisms underlying the physiological processes of P. haitanensis remain unknown. P. haitanensis could utilize inorganic carbon and the sporophytes of P. haitanensis might possess a PCK-type C₄-like carbon-fixation pathway. To identify microRNAs and their probable roles in sporophyte and gametophyte development, we constructed and sequenced small RNA libraries from sporophytes and gametophytes of P. haitanensis. Five microRNAs were identified that shared no sequence homology with known microRNAs. Our results indicated that P. haitanensis might posses a complex sRNA processing system in which the novel microRNAs act as important regulators of the development of different generations of P. haitanensis.     

Strategy of photo-protection in phytoplankton assemblages in the Kongsfjorden,Svalbard, Arctic

Photo-protective functions were investigated in phytoplankton assemblages at Kongsfjorden, Svalbard in spring, using their UV-absorbing compounds (mycosporine-like amino acids (MAAs)), xanthophyll pigments (diadinoxanthin (DD) and diatoxanthin (DT)) and < beta >- dimethylsulphoniopropionate (< beta >_-DMSP). The dominant phytoplankton species in the inner bay were dominated by Phaeocystis spp. and nanoflagellates, while the offshore waters were dominated by Thalassiosira spp. In the inner bay, UVabsorbing compounds and xanthophyll pigments exhibited higher ratios of MAA to chlorophyll a (MAA:chl a ratio), and both DD and DT to chlorophyll a (DD:chl a ratio and DT:chl a ratio), respectively. Thus, the photoprotective-pigments such as DD and DT appear to complement MAAs in the natural phytoplankton assemblage. However, the ratio of < beta >_-DMSP to chlorophyll a (< beta >_-DMSP:chl a ratio) did not show a distinct spatial distribution according to environmental factors or interspecies differences. In this study, we found that photoprotective compounds occurred in a manner dependent on the phytoplankton species composition in Kongsfjorden Bay, where Phaeocystis is the dominant species.     

Protective effects of C-phycocyanin on alcohol-induced acute liver injury in mice

Excessive alcohol consumption leads to liver disease. Extensive evidence suggests that C-phycocyanin (C-PC), a chromophore phycocyanobilin derived from Spirulina platensis, exerts protective effects against chemical-induced organ damage. In this study, we investigated whether C-PC could protect against ethanol-induced acute liver injury. Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), triglyceride (TG), total cholesterol (CHOL), low-density lipoprotein (LDL), liver homogenate malondialdehyde (MDA), superoxide dismutase (SOD) content were measured, and pathological examination of liver sections were examined. C-PC showed obvious inhibitory effects on serum ALT, AST, TG, CHOL, LDL and MDA, and SOD content significantly increased in the liver. The structure of hepatic lobules was clear, liver sinus returned to normal, and liver cell cords were arranged in neat rows. Cloudiness, swelling, inflammatory cell infiltration and spotty necrosis of liver cells were significantly reduced. Therefore, C-PC can significantly protect against ethanol-induced acute liver injury.     

Importance of kelp-derived organic carbon to the scallop <Emphasis Type="Italic">Chlamys farreri</Emphasis> in an integrated multi-trophic aquaculture system

Bivalves and seaweeds are important cleaners that are widely used in integrated multi-trophic aquaculture (IMTA) systems. A beneficial relationship between seaweed and bivalve in the seaweed-based IMTA system has been confirmed, but the trophic importance of seaweed-derived particulate organic materials to the co-cultured bivalve is still unclear. We evaluated the trophic importance of the kelp Saccharina japonica to the co-cultured scallop Chlamys farreri in a typical IMTA farm in Sungo Bay (Weihai, North China). The dynamics of detritus carbon in the water were monitored during the culturing period. The proportion of kelp-derived organic matter in the diet of the co-cultured scallop was assessed via the stable carbon isotope method. Results showed that the detritus carbon in the water ranged from 75.52 to 265.19 μg/L, which was 25.6% to 73.8% of total particulate organic carbon (TPOC) during the study period. The amount of detritus carbon and its proportion in the TPOC changed throughout the culture cycle of the kelp. Stable carbon isotope analysis showed that the cultured scallop obtained 14.1% to 42.8% of its tissue carbon from the kelp, and that the percentages were closely correlated with the proportion of detritus carbon in the water (F =0.993, P= 0.003). Evaluation showed that for 17 000 tons (wet weight) of annual scallop production, the kelp contributed about 139.3 tons of carbon (535.8 tons of dry mass). This confirms that cultured kelp plays a similar trophic role in IMTA systems as it does in a natural kelp bed. It is a major contributor to the detritus pool and supplies a vital food source to filter-feeding scallops in the IMTA system, especially during winter and early spring when phytoplankton are scarce.     

Antioxidant system responses in two co-occurring green-tide algae under stress conditions

Green tides have occurred every year from 2007 to 2014 in the Yellow Sea. Ulva prolifera(Müller) J. Agardh has been identified as the bloom-forming alga,co-occurring with U. intestinalis. We observed distinct strategies for both algal species during green tides. U. prolifera exhibited a high abundance initially and then decreased dramatically,while U. intestinalis persisted throughout. The antioxidant system responses of these two macroalgae were compared in the late phase of a green tide(in-situ) and after laboratory acclimation. Lipid peroxidation and antioxidant system responses differed significantly between the two. Malondialdehyde and hydrogen peroxide contents increased significantly in-situ in U. prolifera,but not in U. intestinalis. In U. prolifera,we observed a significant decrease in total antioxidant ability(T-AOC),antioxidant enzymes(SOD and Apx),and non-enzyme antioxidants(GSH and As A) in-situ. U. intestinalis showed the same pattern of T-AOC and SOD,but its Gpx,Apx,and GSH responses did not differ significantly. The results suggest that U. prolifera was more susceptible than U. intestinalis to the harsh environmental changes during the late phase of a Yellow Sea green tide. The boom and bust strategy exhibited by U. prolifera and the persistence of U. intestinalis can be explained by differences in enzyme activity and antioxidant systems.     

广西近海灾害地质因素及声学反射特征

利用高分辨率地球物理资料及相关历史资料,对北部湾北部的广西近海灾害地质因素进行了地质地球物理解译,识别出海底沙波、海底沙脊、塌陷洼地、冲刷沟槽、浅层气、残积砂体、地层间断、埋藏丘、埋藏古河道等多种灾害地质因素.根据它们出现的频率、规模及声学特征可知:浅层气和埋藏古河道是广西近海最为发育的灾害地质因素,其对海洋工程的施工与建设有较大的危害性.     

From sequential to parallel growth of cities: Theory and evidence from Canada

This paper examines city growth patterns and the corresponding city size distribution evolution over long periods of time using a simple New Economic Geography(NEG) model and urban population data from Canada. The main findings are twofold. First, there is a transition from sequential to parallel growth of cities over long periods of time: city growth shows a sequential mode in the stage of rapid urbanization, i.e., the cities with the best development conditions will take the lead in growth, after which the cities with higher ranks will become the fastest-growing cities; in the late stage of urbanization, city growth converges according to Gibrat′s law, and exhibits a parallel growth pattern. Second, city size distribution is found to have persistent structural characteristics: the city system is self-organized into multiple discrete size groups; city growth shows club convergence characteristics, and the cities with similar development conditions eventually converge to a similar size. The results will not only enhance our understanding of urbanization process, but will also provide a timely and clear policy reference for promoting the healthy urbanization of developing countries.     

A bi-modal model for Chinese cities: City size,car use and land rent

China is experiencing rapid urbanization and motorization. Urban transport congestion poses a challenge to the cities of China. Policies have been made trying to control the car use and the land use in Chinese cities without sound modelling researches. The existing literature on monocentric city modelling has shown that the parameters are critical for the outcome of the modelling. Following the Alonso-type monocentric model, this paper introduces a bi-modal model to simulate the city size, the distribution of land rent and the modal substitution in Chinese cities. We set the key parameters according to the recent available data of China′s cities, and re-explain the hypothesis of the model. Then we make a sensitivity analysis to reveal the impacts of key parameters on the Chinese cities. According to the results, we find that the wage, the price of car use and the agriculture rent have significant impacts on city size. The land supply for the private transport or the public transport has the strongest impacts on car use and the level of transport congestion. The total population of the city and the wage level have strong impacts on land rent. Some results are counter-intuitive, but explainable. We also discuss implication of these results for policy making.     

Wetland economic valuation approaches and prospects in China

Ecosystem services valuation seeks to increase the social relevance of ecosystem characteristics, the underlying biological mechanisms that support services, by making the contribution of ecosystems to human well-being explicit. Economic valuation can help management by clarifying the full range of benefits and costs of proposed management actions. In the past two decades, economic valuation of wetland ecosystem services has become one of the most significant scientific priorities for wetland protection. In this paper, we provide an overview of ecosystem services, and summarize the main interdisciplinary approaches to measure and value wetland ecosystem services. We identified four main methodological gaps preventing progress on wetland valuation of ecosystem services in China, which are: 1) confusion on terminology like intermediate and final ecosystem services, 2) lack of ecological production functions to link ecosystem characteristics to final ecosystem services, 3) static valuation making it difficult to evaluate the trade-offs and synergies among ecosystem services, and 4) lack of clear guidance on relating ecological compensation programs to conservation targets. Overcoming these gaps is important to inform wetland compensation mechanisms and conservation policies. We propose future research on wetland ecosystem services in China should be focused on: 1) defining final ecosystem services based on beneficiary preferences and underlying biophysical mechanisms, 2) establishing wetland monitoring programs at specific sites to collect data on final ecosystem service indicators and ecosystem characteristic metrics to create ecological production functions for economic valuation and rescaling techniques, and 3) incorporating wetland ecosystem service values into decision-making processes to inform wetland management.     

Rank-size distributions of Chinese cities: Macro and micro patterns

A large number of studies have been conducted to find a better fit for city rank-size distributions in different countries. Many theoretical curves have been proposed, but no consensus has been reached. This study argues for the importance of examining city rank-size distribution across different city size scales. In addition to focusing on macro patterns, this study examines the micro patterns of city rank-size distributions in China. A moving window method is developed to detect rank-size distributions of cities in different sizes incrementally. The results show that micro patterns of the actual city rank-size distributions in China are much more complex than those suggested by the three theoretical distributions examined(Pareto, quadratic, and q-exponential distributions). City size distributions present persistent discontinuities. Large cities are more evenly distributed than small cities and than that predicted by Zipf′s law. In addition, the trend is becoming more pronounced over time. Medium-sized cities became evenly distributed first and then unevenly distributed thereafter. The rank-size distributions of small cities are relatively consistent. While the three theoretical distributions examined in this study all have the ability to detect the overall dynamics of city rank-size distributions, the actual macro distribution may be composed of a combination of the three theoretical distributions.