杂色龙虾线粒体基因组全序列的测定与分析

为了解杂色龙虾(Panulirus versicolor)线粒体基因组多态位点及遗传变异,对龙虾类的分类研究奠定基础,作者通过常规PCR步移扩增法获得杂色龙虾线的线粒体基因组全序列,分析了其线粒体基因组的基本特征以及基因排列情况,并结合中国龙虾(Panulirus stimposni)、波纹龙虾(Panulirus homarus)线粒体基因组全序列,综合分析了龙虾属线粒体编码基因多态位点及基因变异状况。结果表明:杂色龙虾线粒体基因组全序列为15 700 bp,由13个蛋白质编码基因、22个t RNA基因、2个r RNA基因和D-loop区组成,保持了泛甲壳动物线粒体基因组的原始排列。杂色龙虾线粒体DNA的碱基组成具有AT偏好性,A+T含量为67%,4种碱基的含量分别为(A=34.6%,T=32.1%,C=20.5%,G=12.8%)。预测了杂色龙虾18个t RNA以及2个r RNA的二级结构。龙虾属线粒体基因组中的nad 2、nad 5基因以及D-loop区的多态位点比例较高,适合作为分子标记用于龙虾类系统进化关系、种内多态性以及遗传分化等方面的研究。     

Numerical investigation of the control factors driving Zhe-Min Coastal Current

During the northeast monsoon season, Zhe-Min Coastal Current(ZMCC) travels along the Chinese mainland coast and carries fresh, cold, and eutrophic water. ZMCC is significantly important for the hydrodynamic processes and marine ecosystems along its path. Thus, this bottom-trapped plume deserves to be further discussed in terms of the major driving factor, for which different opinions exist. For this purpose, in this study, a high resolution Semi-implicit Cross-scale Hydroscience Integrated Syste...     

壳聚糖/纳米铜复合微球的制备及其对刚果红染料的脱色性能

以壳聚糖和CuCl₂·2H₂O为原料,分别采用直接负载法和吸附法成功制备了壳聚糖/纳米铜复合微球,运用傅里叶变换红外光谱(FT-IR)、X-射线衍射(XDR)和扫描电镜(SEM)对催化剂结构进行了表征。以刚果红(CR)染料为目标污染物,评价了催化剂的制备方法、制备条件及氢转移催化反应条件对其催化性能的影响。结果表明,由直接负载法(CS/CuNPs)和吸附法(CS/CuNPs-X)制备的壳聚糖/纳米铜复合微球在CR染料浓度为100 mg/L,催化剂投加量为0.05 g,供氢体浓度为0.05 mol/L的氢转移催化反应条件下,催化反应进行180和60 min时CR脱色率可分别达95.8%和96.8%。CS/CuNPs-X比CS/CuNPs表现了更高的对CR氢转移催化反应的催化速率。两种催化剂催化还原CR染料的反应均符合准一级动力学模型。在连续循环使用10次后,CS/CuNPs和CS/CuNPs-X对CR的脱色率分别为93.9%和89.4%,表明催化剂具有良好的重复使用性能。     

海带褐藻多酚对绿烂海带表面分离的一株细菌的抑菌效果研究

海带苗绿烂病暴发严重威胁海带养殖生产,给海带养殖业造成巨大经济损失.研究发现,海带中的多酚化合物可以抵御海带病害初期病原体侵入.为研究多酚对绿烂海带表面附生菌的抑菌效果,在病烂部位分离出一株细菌,通过16s rRNA基因测序的方法,证实该细菌为小孔芽孢杆菌(Bacillus foraminis).进一步以健康海带为原料...     

北康-曾母盆地中中新世以来层序地层样式特征探讨

开展地震资料解释,分析上超、下超、顶超等地震反射终止关系、不整合面以及沉积趋势,识别出北康-曾母盆地中中新世以来14个三级层序界面。在层序界面内部,通过层序地层内幕结构刻画和原形剖面恢复,并结合地层堆砌方式,在北康-曾母盆地中中新世以来的层序地层内,划分了海进、高位正常海退、强制海退和低位正常海退4种成因单元。通过进一步研究该4种成因单元内地层结构和相分布关系,提出北康-曾母盆地中中新世以来的3种层序地层样式,即陆架边缘富砂型三角洲进积楔、退积型生物礁和陆架边缘富砂-富泥型退积楔,其中,沉积物源供应量充足且可容空间减小时,发育富砂的三角洲进积楔,陆架边缘-斜坡-盆地区域发育含砂深水扇;而可容空间增量远大于沉积物供应量时,发育退积型生物礁和富砂-富泥退积楔,斜坡-盆地区域含砂深水扇欠发育。     

黄河口及邻近海域鱼卵、仔稚鱼群落结构及多样性的年间变化

为了解黄河口及邻近海域鱼卵、仔稚鱼的群落结构动态,分别于2014年、2015年、2017年和2018年的6月利用大型浮游生物网对黄河口及邻近海域进行4个航次的表层水平拖网调查。根据鱼卵、仔稚鱼的种类组成、数量分布等群落结构特征,运用生物多样性指数、聚类分析和皮尔逊相关性矩阵分析等方法对该海域鱼卵、仔稚鱼的资源分布进行了综合研究。结果表明,4年调查共采获鱼卵3 829粒,仔稚鱼234尾,隶属于7目14科15属。鱼卵的主要优势种为鳀（Engraulis japonicus）和短吻红舌鳎（Cynoglossus joyeri）,仔稚鱼的主要优势种为斑鰶（Konosirus punctatus）和虾虎鱼科未定种Gobiidae sp.。生物多样性各指数均以2015年最高,2018年最低。利用R语言对鱼卵、仔稚鱼年间种类组成相似性进行聚类,将4个调查年份划分为3组,各分组之间主要的分歧种均为鳀。生物多样性各指数与环境因子之间皮尔逊相关性分析表明,盐度与鱼卵、仔稚鱼生物多样性的关系较为密切。分析认为,黄河口及邻近海域鱼卵、仔稚鱼群落分布和多样性的年间变化特征体现了鱼类对环境变异的响应。     

三疣梭子蟹(Portunus trituberculatus)foxl2基因功能初探及相关miRNA分析

foxl2在脊椎动物卵巢分化、发育和功能维持等方面具有重要作用,然而其在三疣梭子蟹(Por-tunus trituberculatus)卵巢发育中的功能尚不明确.本研究首先克隆了三疣梭子蟹foxl2(Ptfoxl2)基因cDNA全长序列,该基因5′和3′非编码区域(UTR)长度分别为701?bp和211?bp,开放阅读...     

Application research of narrow band Internet of things buoy and surface hydrodynamics monitoring

This paper applies the narrow band Internet of things communication technology to develop a wireless network equipment and communication system, which can quickly set up a network with a radius of 100 km on water surface. A disposable micro buoy based on narrow-band Internet of things and Beidou positioning function is also developed and used to collect surface hydrodynamic data online. In addition, a web-based public service platform is designed for the analysis and visualization of the data collected by buoys. Combined with the satellite remote sensing data, the study carries a series of marine experiments and studies such as sediment deposition tracking and garbage floating tracking.     

The influence of Stokes drift on oil spills: Sanchi oil spill case

Spilled oil floats and travels across the water’s surface under the influence of wind, currents, and wave action. Wave-induced Stokes drift is an important physical process that can affect surface water particles but that is currently absent from oil spill analyses. In this study, two methods are applied to determine the velocity of Stokes drift, the first calculates velocity from the wind-related formula based upon a one-dimensional frequency spectrum, while the second determines velocity directly from the wave model that was based on a two-dimensional spectrum. The experimental results of numerous models indicated that: (1) oil simulations that include the influence of Stokes drift are more accurate than that those do not; (2) for medium and long-term simulations longer than two days or more, Stokes drift is a significant factor that should not be ignored, and its magnitude can reach about 2% of the wind speed; (3) the velocity of Stokes drift is related to the wind but is not linear. Therefore, Stokes drift cannot simply be replaced or substituted by simply increasing the wind drift factor, which can cause errors in oil spill projections; (4) the Stokes drift velocity obtained from the two-dimensional wave spectrum makes the oil spill simulation more accurate.     

大陆岛入海沉积物通量的估算研究

大陆岛入海沉积物通量（Q_s）信息对于精确解译大陆架沉积记录的研究是个重要补充。针对如何估算大陆岛Q_s的科学问题,本文以中国东南部海域的8 227个大陆岛为例,提出了一种基于邻近大陆中小型河流Q_s的经验公式计算大陆岛Q_s的解决方案。该方案在实施时需设置两个假定,即大陆岛的Q_s法则遵循邻近大陆中小河流的Q_s法则和可将1个大陆岛当作1个河流流域计算其Q_s。结果表明：（1）经验公式计算的大陆岛Q_s为其最小估计值;如考虑大陆岛流域的具体情况,实际的Q_s值会稍微增加,但其增幅不超过n^0.13（n为流域数量）;（2）经验公式能获取大陆岛Q_s的大致数量级信息;大陆岛的总面积为4 418.49 km²,对应Q_s的数量级为10⁶ t/a,与邻近大陆中型河流入海通量的数量级相当;（3）在大河河口湾充填阶段完成以前,大陆岛沉积物是内陆架泥质沉积体的主要物源之一。因此,大陆岛入海沉积物会对大陆架沉积体系的形成和演化造成一定程度的影响,需引起研究人员的高度重视。