不同光生物反应器培养坛紫菜 丝状体的比较研究

Vegetative cells of Porphyra haitanensis sporophytes were grown in a bubble-column and a draft-tube internal-loop airlift bioreactor at 20℃ with an illuminance of 40μmol photons m-2 s-1 for 20days. The draft-tube internal-loop airlift bioreactor yielded maximum cell density of 4850mg DCW (Dried Cell Weight)/L and higher growth rate of 0.094/d, compared to the bubble-column, 3950mg DCW/L and 0.086/d, respectively. The advantages were related to the more homogeneous fluid dynamic characteristics in the draft-tube internal-loop airlift bioreactor than the bubble-column bioreactor.     

网形不一致的工程控制网稳定性分析

对于施工周期较长的大型工程,通常需要对控制网进行定期复测和稳定性分析。控制网复测期间,有时会遇到点位破坏或增设新点等情况,从而使两期控制网的网形不一致。针对控制网无固定起算点和网形改变的问题,讨论控制网的稳定性分析方法,并进行实例分析,为控制网的正确使用提供重要保证。     

地理信息服务的思考与探索

地理信息系统(geographic information system,GIS)正在从传统意义上的地理信息系统向地理信息服务偏转,地理信息服务已成为信息服务业中的一个重要组成部分.文中归纳总结地理信息服务概念、技术体系、服务模式以及我国地理信息服务发展的差距、对策与建议.     

Spatial variations of dimethylsulfide and dimethylsulfoniopropionate in the surface microlayer and in the subsurface waters of the South China Sea during springtime

Spatial variations in dimethylsulfide (DMS) and dimethylsulfoniopropionate (DMSP) were surveyed in the surface microlayer and in the subsurface waters of the low productivity South China Sea in May 2005. Overall, average subsurface water concentrations of DMS and DMSP of dissolved (DMSPd) and particulate (DMSPp) fractions were 1.74 (1.00-2.50), 3.92 (2.21-6.54) and 6.06 (3.40-8.68) nM, respectively. No enrichment in DMS and DMSPp was observed in the microlayer. In contrast, the microlayer showed a DMSPd enrichment, with an average enrichment factor (EF, defined as the ratio of the microlayer concentration to subsurface water concentration) of 1.40. In the study area, none of the sulfur components were correlated with chlorophyll a. An important finding in this study was that DMS, DMSP and chlorophyll a concentrations in the surface microlayer were respectively correlated with those in the subsurface water, suggesting a close linkage between these two water bodies. The ratios of DMS:Chl-a and DMSPp:Chl-a showed a gradually increasing trend from North to South. This might be due to changes in the proportion of DMSP producers in the phytoplankton community with the increased surface seawater temperature. A clear diurnal variation in the DMS and DMSP concentrations was observed at an anchor station with the highest concentrations appearing during the day and the lowest concentrations during the night. The higher DMS and DMSP concentrations during daytime might be attributed to the light-induced increase in both algal synthesis and exudation of DMSP and biological production of DMS. The mean flux of DMS from the investigated area to the atmosphere was estimated to be 2.06 micromo lm(-2)d(-1). This low DMS emission flux, together with the low DMS surface concentrations was attributed to the low productivity in this sea.     

The role of horizontal impulses of the faulting continental slope in generating the 26 December 2004 tsunami

For a long time, people have believed that the vertical displacement of seafloor due to undersea earthquakes is the primary cause of tsunami genesis. However, seismically-inverted seafloor deformation of the 2004 Sumatra–Andaman earthquake shows that the total vertical displacement is not enough to have generated the powerful Indian Ocean tsunami. Based on the seismically-inverted data and a three-dimensional ocean general circulation model (OGCM), we show that the momentum force, transferred by the horizontal impulses of the faulting continental slope in that earthquake, has accounted for two thirds of the satellite-observed tsunami height and generated kinetic energy 5 times larger than the potential energy due to the vertical displacement. The asymmetric tsunami pattern, recorded by tide-gauges showing leading-elevation waves toward Sri Lanka and leading-depression waves toward Thailand, is best explained by the horizontally-forced mechanism. The same mechanism has also explained the March 2005 Nias earthquake and tsunami data, suggesting that the horizontal motions of faulting have played more important roles in tsunami genesis than previously thought.     

应用测量技术解决城市地铁与地下管线影响问题的研究

城市地铁和地下管线是城市设施的两个重要内容,二者同处地下空间,相互影响,测量是解决二者空间冲突和安全建设、运营的重要技术手段。通过分析城市地铁与地下管线在设计、施工及运营阶段的影响关系,提出了解决问题的措施,介绍了解决措施中的关键技术问题,为地铁建设、运营对地下管线的影响等有关问题提供了解决思路。     

壳聚糖硫酸酯金属配合物的抗氧化活性

以吩嗪硫酸甲酯-NADH为超氧阴离子自由基(O_2~(·-))产生、检测体系和EDTANa_2-Fe(Ⅱ)-H_2O_2为羟自由基(~·OH)产生、检测体系,对壳聚糖硫酸酯铜、锌配合物和不同分子质量壳聚糖进行了抗O_2~(·-)和~·OH自由基的活性研究.结果表明,壳聚糖硫酸酯金属配合物对于O_2~(·-)自由基的清除能力明显高于壳聚糖,在质量浓度为0.025 g/L时,壳聚糖硫酸酯铜配合物对O_2~(·-)自由基的清除能力达到94.18%,壳聚糖硫酸酯锌配合物达到93.19%;壳聚糖硫酸酯铜、锌配合物对~·OH自由基的清除能力(67.39%、60.46%)低于相同分子质量的壳聚糖(88.06%),而高于高分子质量壳聚糖761 ku(18.71%);壳聚糖分子质量大小对O_2~(·-)和~·OH自由基的清除能力有较大影响,质量浓度为1.6 g/L壳聚糖分子质量为20 ku时,对O_2~(·-)清除率达54.69%,而分子质量在761 ku时,对O_2~(·-)清除率仅为35.50%;各样品对O_2~(·-)和~·OH自由基的清除能力均随着质量浓度的增加而上升,壳聚糖硫酸酯铜、锌配合物在相当低的浓度下(0.025 g/L)就可以达到明显清除O_2~(·-)自由基的效果(≥90%).     

雨生红球藻八氢番茄红素脱氢酶pds基因上游序列的分离和分析

在某些蓝藻、绿藻和高等植物中,八氢番茄红素脱氢酶是β-胡萝卜素合成过程中的一个关键酶之一,应用巢式PCR方法从雨生红球藻中克隆了八氢番茄红素脱氢酶基因约1kb的5’上游序列。通过生物信息学方法进行序列分析,发现pds基因上游序列中包含了一些可能的顺式元件,如ABRE调控元件、C-repeat/DRE调控元件等。同时,构建了由pds-启动子控制报告基因的重组载体,并转化到雨生红球藻细胞中,进行了报告基因瞬间表达的检测。结果表明,克隆的pds启动子区域具有启动子活性,可以驱使报告基因进行瞬间表达。     

基于Global Mapper的地貌晕渲制图——以西安幅(Ⅰ-49)为例

地貌晕渲图自动制图是以计算机软、硬件为基础,对DEM数据实现的一种三维立体可视化表达。本文从地貌晕渲的基本理论出发,以我国1:100万标准分幅的西安幅(1-49)为例,采用Global Mapper软件实现了计算机地貌晕渲自动制作的全过程,主要内容包括:计算机地貌晕渲关键技术的实现,地貌晕渲效果与参数设置之间的关系,Global Mapper软件中各种参数设置的合理性分析,使用分层设色的办法对晕渲图进行色彩的设计,并利用PHOTOSHOP软件对结果进行整饰等。该方法可实现地貌晕渲图的自动制图。     

基于GIS的零售商业网点选址评价模型研究

传统零售商业网点选址模型主要是基于统计的静态模型.分析了影响零售商业网点选址的人口、经济、市场竞争三个关键要素,结合GIS,提出了一种基于权重设置的零售商业网点选址评价模型,并阐述了模型的应用流程.该模型在深圳市罗湖区公共信息服务平台中进行了典型应用实现,并检验了模型的应用可行性.