藓羽藻(Bryopsis hypnoides)核酮糖-1，5-二磷酸羧化酶／加氧酶的分离与活性测定

采用硫酸铵分部沉淀与凝胶过滤的方法，进行藓羽藻Rubisco的分离研究。结果表明，分离的藓羽藻Rubisco经SDS-聚丙烯酰胺凝胶电泳检测呈两条清晰条带，分别为Rubisco大亚基与小亚基；与菠菜相比，藓羽藻Rubisco大亚基分子量与菠菜基本相同，而小亚基较之稍大一些。藓羽藻Rubisco活力测定结果表明，Rubisco分离过程中用硫酸铵分部沉淀后活力降低许多，分离后活力有所上升，但仍比粗提液活力弱；在Rubisco活力测定过程中，藓羽藻Rubisco的活化温度与其它物种Rubisco活化的温度不同，在低温下活化效果较好。这些结果说明Rubisco的酶活力受硫酸铵的影响而且藓羽藻Rubisco相对陆地高等植物结构不稳定。     

东海沿岸海区垂直环流及其温盐结构动力过程研究Ⅱ．温盐结构

对于本研究采用的动力学模型及其垂直环流的模拟结果已在第Ⅰ部分论述。作者将对与垂直环流对应的温、盐结构进行分析。温度和盐度模拟结果表明：冬季东海沿岸海区的温、盐分布均为近岸低、外海高;近岸温、盐呈垂直均匀分布,在外海出现分层,其温度为表层高、底层低,而盐度却为表层和底层高,中层偏低,长江口以南的近表层以下形成自近岸伸向外海的弱低盐水舌;长江冲淡水区及长江口以北和其以南外海的近表层有温、盐跃层生成,深底层温、盐呈均匀分布,且保持低温高盐特征;随着海区自北往南纬度的降低,岸坡和水深的增大及沿岸下降流的增强,温度和盐度自近岸至外海的垂直均匀分布跨度逐渐变窄;外海近表层的温、盐跃层强度自北至长江口逐渐增强,而自长江口至南逐渐减弱,其位置自北往南逐渐上移;冬季沿岸下降流使长江冲淡水区的盐跃层变厚。夏季海区的温度分布为近岸和外海高,近岸稍远出现冷水涌升,垂向上呈现显著分层,盐度分布为近岸低、外海高;长江冲淡水区及杭州湾以南外海的次表层存在温、盐跃层,其跃层以上出现混合层,且保持高温低盐特征,跃层以下温、盐大致呈均匀分布,并保持低温高盐特征;随着海区自南往北纬度的增高、岸坡和水深的减小及沿岸上升流自南至长江口和自长江口至北的增强和继而减弱,长江冲淡水区的温、盐跃层强度自南至长江口逐渐增强,而自长江口至北逐渐减弱,外海次表层的温、盐跃层强度却自南至长江口逐渐减弱,自长江口至北又逐渐增强,其温、盐跃层的位置自南往北逐渐上移;夏季沿岸上升流使长江冲淡水区的盐跃层变薄。     

Genetic relationship between Neobenedenia girellae and N. melleni inferred from 28S rRNA sequences

The fragments of 350 bp in 28S rRNA from the closely related monogenea of trematoda, Neobenedenia girellae and N. melleni are obtained by polymerase chain reaction (PCR) amplified using a couple of special primers and then sequenced. The results show that the comparison of 28S rRNA sequences, with only a base varying in 337 bp accounting for 0.3% genetic difference, from the relative species N. girellae and N. melleni parasitized on the different fishes in different farms displays that they possess a very high genetic similarity of 99.7%, higher than that of 99.41% for the single species N. melleni sampled in different areas, and the intraspecific divergence of N.melleni is 0.59%. Meanwhile, the interspecific differences between the two Neobenedenia and three Benedenia (i.e., B. lutjani, B. rohdei and B. seriolae) range from 2.08% tol 1.73%. In addition, UPGMA and MP molecular phylogenetic trees are constructed and proved to be consistent with each other. Though the morphological characteristics and the results of genetic diversity for the two Neobenedenia show a high similarity, whether they belong to a single species or not are still undefined, and the more genes of them should be further investigated, in combination with the systematical and detailed morphological study.     

冲绳海槽天然气水合物BSR的地震研究

根据多道地震反射资料分析,在冲绳海槽南部和中部发现了拟海底反射层(BSR)现象。通过对海底异常反射层的振幅特征、速度异常和AVO属性分析,说明该BSR可能反映了天然气水合物的存在,并发现冲绳海槽断层与天然气水合物的形成有密切关系。     

南海“耗氧”及“贫氧”水体中消耗氧与硝酸盐和磷酸盐再生比值估算

通过对南海深海区硝酸氮、活性磷酸盐垂直分布的研究,分析了南海垂向耗氧和贫氧水体中硝酸氮、活性磷酸盐和表观耗氧量之间的关系.结果表明垂向上次表层、中层和深层水团中氧的消耗与营养盐再生比值均小于理论值,由此推断水体的混合为有机物再矿化提供氧的补充,但水团混合对水柱中硝酸氮、活性磷酸盐的影响不大,在次表层和中层水团中造成营养盐浓度变化主要是由于有机物再矿化的结果;在深层水团中有机物再矿化对水柱中营养盐梯度变化已不占主导地位.     

下辽河平原—辽东湾的新构造运动

通过地质、地貌及地球物理资料分析研究,发现下辽河平原—辽东湾的新构造运动的主要特征除了断裂发育、火山活动早期剧烈而晚期衰弱、现代地震活跃以及地壳差异和反差升降运动明显之外,还显示了:(1)深大断裂的控制性,(2)沉降中心的迁移性,(3)构造运动的阶段性和旋回性.     

Effect of the Twin Jetties as A Regulating Structure at the Tao-er Estuary

The phase one project of the twin jetties at the Tao-er Estuary yields no effect on the flow, and there is a large area of deposition. A numerical model is employed to simulate the flow field, and the scouring and silting in the channel be-tween the two jetties are analyzed. The results show that the effective tidal flux between the two jetties is reduced due to the notch located at the heel of the east jetty, and the effect of contraction-flush is not obvious. As for the regulation of this kind of estuary under the tide action, the capability of tidal flux should be fully used.     

1998年春夏南海温盐结构及其变化特征

利用1998年5～8月“南海季风试验”期间“科学1”号和“实验3”号科学考察船两个航次CTD资料,分析了1998年南海夏季风暴发前后南海主要断面的温盐结构及其变化特征.观测发现,南海腹地基本被典型的南海水团所控制,但在南海东北部尤其是吕宋海峡附近,表层和次表层水明显受到西太平洋水的影响.季风暴发以后,南海北部表面温度有显著升高,升幅由西向东递减,而南海中部和南部表面温度基本没变,这使得南海北部东西向温度梯度和整个海盆南北向温度梯度均减小.北部断面表层盐度普遍由34以上降低到34以下,混合层均有所发展,是季风暴发后降水和风力加剧的结果.观测期间黑潮水跨越吕宋海峡的迹象明显但变化剧烈.4～5月,黑潮次表层水除在吕宋海峡中北部出现外,在吕宋岛以西亦有发现,表明有部分黑潮水从吕宋海峡南端沿岸向西进而向南进入南海.6～7月,次表层高盐核在吕宋海峡中北部有极大发展,但在吕宋岛以西却明显萎缩;虽然看上去黑潮水以更强的流速进、出南海,但对南海腹地动力热力结构的影响未必更大.一个超过34.55的表层高盐水体于巴拉望附近被发现,似与通过巴拉望两侧水道入侵南海的西太平洋水有关.     

南海北部海区上层水体平均声速场的变化

对所收集到的南海北部海区的温度、盐度和深度历史资料进行了声速的计算、统计与分析,结果表明该海区上层水体的平均声速场有较明显的年际变化:表层声速的平面分布显示出沿岸水与外海水强弱交替变化的特征,声速等值线的走向几乎与岸线平行,等声速线的值自近岸向外海增加,大陆架外缘海区声速的水平梯度较大;下层声速的分布以环流和水体共同作用的形式出现,50m层声速平面分布的趋势除冬季与表层稍为相似外,其余季节与表层有明显的差别,春、夏季节50m层声速自西向东增加,而秋季与其表层分布相反,自近岸向外海减小;声速的垂直分布受海水升温与降温的影响显著,春、夏季表层海水升温,海表声速最大,声速自海表随深度的增加而减小;秋、冬季表层附近水层降温,声速稍偏低,普遍出现正梯度现象,最大声速移至表层以下的水层,这个深度随季节的改变而改变,随海区的不同而不同.该海区的平均声速有年波动现象,表层波幅最大,随深度的增加波幅变小,至100m水层其波动的位相几乎与表层相反.     

Numerical Simulation of Non-Linear Wave Propagation in Waters of Mildly Varying Topography with Complicated Boundary

In this paper, the characteristics of different forms of mild slope equations for non-linear wave are analyzed, and new non-linear theoretic models for wave propagation are presented, with non-linear terms added to the mild slope equations for non-stationary linear waves and dissipative effects considered. Numerical simulation models are developed of non-linear wave propagation for waters of mildly varying topography with complicated boundary, and the effects are studied of different non-linear corrections on calculation results of extended mild slope equations. Systematical numerical simulation tests show that the present models can effectively reflect non-linear effects.