The solitary ascidian, Ciona savignyi (Ascidiacea, Enterogona) is a notorious marine invader still expanding its habitat range worldwide. This species is considered native to the North West Pacific, but its indigeneity in Korean coastal waters has been questioned because of outdated taxonomic records and its inhabitation of oceanographically marginal areas. To clarify their cryptic invasion state, 247 individual C. savignyi samples were collected from 12 harbors and marinas on the Korean coast, and a 744 bp region of mitochondrial DNA (mtDNA) cytochrome c oxidase subunit I gene was sequenced and analyzed. Our analyses of population genetic structure and demographic history provided considerable pieces of evidence supporting their long-term establishment on the Korean coasts: differentiated population genetic structure, sequentially arrayed star-shape haplotype network, neutrality test results of past population expansions, and post-glacial colonization pattern of demography. Consequently, we concluded that C. savignyi populations on the Korean Coast are indigenous rather than exotic. These results could be used as reference data for further phylogeo graphic and demographic studies of problematic Ciona species, and to clarify and resolve similar cryptic invasion states of the other Korean coastal marine organisms. This study is the first to resolve the cryptic in vasion state of Korean marine organisms using genetic analysis.
AbstractThis study developed prestressed high-strength concrete (PHC) piles reinforced with high-strength materials (glass fiber-reinforced polymer (GFRP) bars) for flexural performance enhancement. Flexural strengths and behaviors of PHC piles reinforced with hybrid GFRP and steel bars were experimentally investigated, respectively. Large-scale specimens with total lengths of 12,000?mm and diameters of 600?mm were constructed and tested under bending, accompanied by evaluation of effects of non-prestressed reinforcement type and longitudinal reinforcement ratio. J-factors were calculated to evaluate deformability of all the specimens. PHC piles reinforced with GFRP bars were demonstrated to have much higher flexural capacity than those reinforced with steel bars. Moreover, strains at the midspans of cross sections of all the specimens basically conformed to the assumption of plane section. Failure of PHC piles reinforced with GFRP bars was attributable to gradual concrete crushing, while that of PHC piles reinforced with steel bars resulted from steel yielding. Results of this study were expected to provide theoretical basis for wide engineering applications of PHC piles reinforced with hybrid GFRP bars and steel bars in marine structures. 相似文献