Microalgae, a sustainable source of multi beneficial components has been discovered and could be utilised in pharmaceutical, bioenergy and food applications. This study aims to investigate the sugaring-out effect on the recovery of protein from wet green microalga, Chlorella sorokiniana CY1 which was assisted with sonication. A comparison of monosaccharides and disaccharides as one of the phaseforming constituents shows that the monosaccharides, glucose was the most suitable sugar in forming the phases with acetonitrile to enhance the production of protein(52% of protein). The protein productivity of microalgae was found to be significantly influenced by the volume ratio of both phases, as the yield of protein increased to 77%. The interval time between the sonication as well as the sonication modes were influencing the protein productivity as well. The optimum protein productivity was obtained with 10 s of resting time in between sonication. Pulse mode of sonication was suitable to break down the cell wall of microalgae compared to continuous mode as a lower protein yield was obtained with the application of continuous mode. The optimum condition for protein extraction were found as followed: 200 g/L glucose as bottom phase with volume ratio of I:1.25, 10 s of resting time for ultrasonication, 5 s of ultrasonication in pulse mode and 0.25 g of biomass weight. The high yield of protein about 81% could be obtained from microalgae which demonstrates the potential of this source and expected to play an important role in the future. 相似文献
Based on in-situ observation, satellite and reanalysis data, responses of the western North Pacific subtropical ocean(WNPSO) to the slow-moving category 5 super typhoon Nanmadol in 2011 are analyzed. The dynamical response is dominated by near-inertial currents and Ekman currents with maximum amplitude of 0.39 m/s and 0.15 m/s, respectively. The near-inertial currents concentrated around 100 m below the sea surface and had an e-folding timescale of 4 days. The near-inertial energy propagated both upward and downward, and the vertical phase speed and wavelength were estimated to be 5 m/h and 175 m, respectively. The frequency of the near-inertial currents was blue-shifted near the surface and redshifted in ocean interior which may relate to wave propagation and/or background vorticity. The resultant surface cooling reaches -4.35℃ and happens when translation speed of Nanmadol is smaller than 3.0 m/s.When Nanmadol reaches super typhoon intensity, the cooling is less than 3.0℃ suggesting that the typhoon translation speed plays important roles as well as typhoon intensity in surface cooling. Upwelling induced by the slow-moving typhoon wind leads to typhoon track confined cooling area and the right-hand bias of cooling is slight. The mixed layer cooling and thermocline warming are induced by wind-generated upwelling and vertical entrainment. Vertical entrainment also led to mixed layer salinity increase and thermocline salinity decrease, however, mixed layer salinity decrease occurs at certain stations as well. Our results suggest that typhoon translation speed is a vital factor responsible for the oceanic thermohaline and dynamical responses, and the small Mach number(slow typhoon translation speed) facilitate development of Ekman current and upwelling. 相似文献
Decapterus maruadsi is a commercially important species in China, but has been heavily exploited in some areas. There is a growing need to develop microsatellites promoting its genetic research for the adequate management of this fishery resources. The recently developed specific-locus amplified fragment sequencing (SLAF-seq) is an efficient and high-resolution method for genome-wide microsatellite markers discovery. In this study, 28 905 microsatellites (mono- to hexa-nucleotide repeats) were identified using SLAF-seq technology, of which di-nucleotide was the most frequent (13 590, 47.02%), followed by mono-nucleotide (8 138, 28.15%), tri-nucleotide (5 727, 19.81%), tetra-nucleotide (1 104, 3.82%), pentanucleotide (234, 0.81%), and hexa-nucleotide (112, 0.39%). One hundred and thirty-two microsatellite loci (di- and tri-nucleotide) were randomly selected for amplification and polymorphism, of which 49 were highly polymorphic and well-resolved. The average number of alleles per locus was 13.63, ranging from 4 to 25, and allele sizes varied between 110 bp and 309 bp. The observed heterozygosity ( Ho ) and expected heterozygosity ( He ) ranged from 0.233 to 1.000 and from 0.374 to 0.959, with mean values of 0.738 and 0.836, respectively. The polymorphism information content (PIC) ranged from 0.341 to 0.941 (mean=0.806). However, 12 loci deviated from Hardy-Weinberg equilibrium. Furthermore, transferability tests were also successful in validating the utility of the developed markers in five phylogenetically related species of family Carangidae. A total of 48 microsatellite markers were successfully cross-amplified in Decapterus macarellus, Decapterus macrosoma, Decapterus kurroides, Trachurus japonicus, and Selaroides leptolepis. The present microsatellites provided the first known set of microsatellite DNA markers for D. maruadsi, D. macarellus, D. kurroides, and D. macrosoma, and would be useful for further population genetic and molecular phylogeny studies as well as help with the fisheries management formulation and implementation of the understudied species.