Isolation of protein from Chlorella sorokiniana CY1 using liquid biphasic flotation assisted with sonication through sugaring-out effect

Microalgae, a sustainable source of multi beneficial components has been discovered and could be utilised in pharmaceutical, bioenergy and food applications. This study aims to investigate the sugaring-out effect on the recovery of protein from wet green microalga, Chlorella sorokiniana CY1 which was assisted with sonication. A comparison of monosaccharides and disaccharides as one of the phaseforming constituents shows that the monosaccharides, glucose was the most suitable sugar in forming the phases with acetonitrile to enhance the production of protein(52% of protein). The protein productivity of microalgae was found to be significantly influenced by the volume ratio of both phases, as the yield of protein increased to 77%. The interval time between the sonication as well as the sonication modes were influencing the protein productivity as well. The optimum protein productivity was obtained with 10 s of resting time in between sonication. Pulse mode of sonication was suitable to break down the cell wall of microalgae compared to continuous mode as a lower protein yield was obtained with the application of continuous mode. The optimum condition for protein extraction were found as followed: 200 g/L glucose as bottom phase with volume ratio of I:1.25, 10 s of resting time for ultrasonication, 5 s of ultrasonication in pulse mode and 0.25 g of biomass weight. The high yield of protein about 81% could be obtained from microalgae which demonstrates the potential of this source and expected to play an important role in the future.     

Response of the western North Pacific subtropical ocean to the slow-moving super typhoon Nanmadol

Based on in-situ observation, satellite and reanalysis data, responses of the western North Pacific subtropical ocean(WNPSO) to the slow-moving category 5 super typhoon Nanmadol in 2011 are analyzed. The dynamical response is dominated by near-inertial currents and Ekman currents with maximum amplitude of 0.39 m/s and 0.15 m/s, respectively. The near-inertial currents concentrated around 100 m below the sea surface and had an e-folding timescale of 4 days. The near-inertial energy propagated both upward and downward, and the vertical phase speed and wavelength were estimated to be 5 m/h and 175 m, respectively. The frequency of the near-inertial currents was blue-shifted near the surface and redshifted in ocean interior which may relate to wave propagation and/or background vorticity. The resultant surface cooling reaches -4.35℃ and happens when translation speed of Nanmadol is smaller than 3.0 m/s.When Nanmadol reaches super typhoon intensity, the cooling is less than 3.0℃ suggesting that the typhoon translation speed plays important roles as well as typhoon intensity in surface cooling. Upwelling induced by the slow-moving typhoon wind leads to typhoon track confined cooling area and the right-hand bias of cooling is slight. The mixed layer cooling and thermocline warming are induced by wind-generated upwelling and vertical entrainment. Vertical entrainment also led to mixed layer salinity increase and thermocline salinity decrease, however, mixed layer salinity decrease occurs at certain stations as well. Our results suggest that typhoon translation speed is a vital factor responsible for the oceanic thermohaline and dynamical responses, and the small Mach number(slow typhoon translation speed) facilitate development of Ekman current and upwelling.     

Isolation and characterization of 49 polymorphic microsatellite loci for Decapterus maruadsi using SLAF-seq,and cross-amplification to related species

Decapterus maruadsi is a commercially important species in China, but has been heavily exploited in some areas. There is a growing need to develop microsatellites promoting its genetic research for the adequate management of this fishery resources. The recently developed specific-locus amplified fragment sequencing (SLAF-seq) is an efficient and high-resolution method for genome-wide microsatellite markers discovery. In this study, 28 905 microsatellites (mono- to hexa-nucleotide repeats) were identified using SLAF-seq technology, of which di-nucleotide was the most frequent (13 590, 47.02%), followed by mono-nucleotide (8 138, 28.15%), tri-nucleotide (5 727, 19.81%), tetra-nucleotide (1 104, 3.82%), pentanucleotide (234, 0.81%), and hexa-nucleotide (112, 0.39%). One hundred and thirty-two microsatellite loci (di- and tri-nucleotide) were randomly selected for amplification and polymorphism, of which 49 were highly polymorphic and well-resolved. The average number of alleles per locus was 13.63, ranging from 4 to 25, and allele sizes varied between 110 bp and 309 bp. The observed heterozygosity ( H_o ) and expected heterozygosity ( H_e ) ranged from 0.233 to 1.000 and from 0.374 to 0.959, with mean values of 0.738 and 0.836, respectively. The polymorphism information content (PIC) ranged from 0.341 to 0.941 (mean=0.806). However, 12 loci deviated from Hardy-Weinberg equilibrium. Furthermore, transferability tests were also successful in validating the utility of the developed markers in five phylogenetically related species of family Carangidae. A total of 48 microsatellite markers were successfully cross-amplified in Decapterus macarellus, Decapterus macrosoma, Decapterus kurroides, Trachurus japonicus, and Selaroides leptolepis. The present microsatellites provided the first known set of microsatellite DNA markers for D. maruadsi, D. macarellus, D. kurroides, and D. macrosoma, and would be useful for further population genetic and molecular phylogeny studies as well as help with the fisheries management formulation and implementation of the understudied species.

     

长白山景区旅游安全风险动态评价研究

以长白山景区旅游安全为研究对象,以鱼骨图、动态贝叶斯、GIS技术等为基本研究方法,从研究区自然环境、社会环境及责任人为3个方面出发,筛选景区致险因子,构建景区旅游安全风险危险性评价指标体系,利用动态贝叶斯方法综合构建景区旅游安全风险动态评价模型;并以实测数据及景区统计数据为依据,划分景区旅游安全风险评价的4个动态时段,综合实现景区旅游安全风险动态风险评价。研究结果表明：① 中等以上风险区域呈条带状分布;② 高风险区域与主要景点重合;③ 长白山景区安全风险发生高概率的时段发生在第三个时段（12:00~14:00）;较高概率发生分别在第二个时段（10:00~12:00）与第四个时段（14:00~16:00）;中等概率发生较高的时段在第四个时段（14:00~16:00）;较低概率发生在第一个时段（8:00~10:00）。     

中国人海经济系统环境适应性演化及预警

基于敏感性-稳定性-响应3维要素构建指标体系,运用熵值法和ARIMA-BP组合预测模型研究中国人海经济系统环境适应性的演化及预警。结果表明：① 2001~2016年中国人海经济系统环境适应性呈稳定上升态势,总体集中于中警状态,期间经历了“人海环境系统比较优势阶段→耦合协调阶段→人海经济系统比较优势阶段”的双螺旋适应过程,预计2017~2020年再次进入相互契合的轻警状态;② 16 a间中国人海经济系统环境适应性波动存在上升期短-衰退期长现象,预计未来4 a人海经济系统环境适应性在经济下行和生态约束背景下的速率不容乐观;③ 权衡人海经济系统和人海环境系统的关系,追求总体效益最大化,延长适应性周期波动中扩张期活动,差别化和灵活性的适应行为是未来主要排警对策。     

浮力装置触发深海管道水平向整体屈曲效果的数值模拟研究

海洋油气资源的运输主要通过海底管道进行,管道在工作时受到较大的温度荷载,会产生整体屈曲变形。深海管道设计中常采用人为装置触发一定程度的水平向整体屈曲变形,来释放轴向的温度应力,浮力装置是常用的触发方式之一。本文通过数值模拟研究,分析了不同浮力大小和不同浮力施加范围下,管道水平向整体屈曲的临界屈曲力,得出临界屈曲力随浮力大小和施加范围变化的情况;并研究了不同土体阻力下,浮力装置触发整体屈曲的效果。研究表明,水平向土体阻力较大时,浮力装置触发水平向整体屈曲的效果较好。浮力装置的触发效果对轴向土体阻力不敏感。     

应用Hydrolight模式研究含油水体辐射传输特性的关键技术

辐射传输模式Hydrolight在国际水色遥感研究领域已被广泛用于解决水体光学的各种问题,也是进行石油类污染水体辐射传输特性研究的有效模型。结合Hydrolight模式的机理、应用现状及含油水体的特点,提出了将该模式应用于光学特性复杂的石油污染水体辐射传输特性研究时需要解决的关键技术,包括测定石油污染水体吸收系数和散射系数、建立石油污染吸收和散射特性随波长和深度变化的参数化模型、确定油物质的吸附后悬浮颗粒物体散射函数模型等方面。依据2018年8月和2016年8月分别在辽宁大连港和辽宁盘锦辽河油田等区域测定的含油水体数据,讨论了这些关键技术的解决方案。     

绿潮暴发对浮游动物群落结构影响研究

本调查于2016年和2017年的5月、8月,对日照近岸海域浒苔暴发区进行了4个航次的浮游动物断面调查。4个航次中,2016年和2017年5月航次获得的种类数要明显低于同年8月航次,而平均丰度和湿重生物量均高于同年8月航次。造成这种情况的原因可能是调查海域8月浒苔暴发结束,浒苔的消亡过程会大量消耗营养盐,导致水体中营养盐含量下降,进而影响到其他浮游植物和浮游动物的生长。应用PRIMER软件中的单变量分析得到群落种数(S)、丰富度(d)、香农-威纳指数(Shannon-Weaner index)(H′)和均匀度(J),从多样性指数分析可以看出,5月航次的生物多样性均劣于同年8月航次。不同年度相同季节航次中出现的优势种极其相似,而不同季节航次出现的优势种则大不相同。     

辽宁省海洋服务新业态培育与法律保障研究

海洋服务业已成为海洋经济发展的重要引擎,"十三五"时期是辽宁省海洋经济加快转型升级的关键期,要求培育新动能、发展新模式。因此,培育海洋服务新业态对辽宁省海洋经济发展具有重要意义。文章针对辽宁海洋服务业发展滞后,规模偏小、区域发展不平衡,同质化严重、科技含量低,层次相对低下、与其他产业融合不足,资源整合较差等问题,提出培育海洋服务新业态重要路径为:积极发展四大产业,提高经济规模总量;优化产业区域布局,形成错位发展;提高科技含量,提升海洋服务业层次;促进海洋服务业与其他行业融合协调,提升资源整合度。法律保障措施为:完善法规体系、严格项目审批、提高执法水平、健全应急管理。     

赤道东印度洋和孟加拉湾障碍层厚度的季节内和准半年变化

利用2002—2015年ARGO网格化的温度、盐度数据, 结合卫星资料揭示了赤道东印度洋和孟加拉湾障碍层厚度的季节内和准半年变化特征, 探讨了其变化机制。结果表明, 障碍层厚度变化的两个高值区域出现在赤道东印度洋和孟加拉湾北部。在赤道区域, 障碍层同时受到等温层和混合层变化的影响, 5—7月和11—1月受西风驱动, Wyrtki急流携带阿拉伯海的高盐水与表层的淡水形成盐度层结, 同时西风驱动的下沉Kelvin波加深了等温层, 混合层与等温层分离, 障碍层形成。在湾内, 充沛的降雨和径流带来的大量淡水产生很强的盐度层结, 混合层全年都非常浅, 障碍层季节内变化和准半年变化主要受等温层深度变化的影响。上述两个区域障碍层变化存在关联, 季节内和准半年周期的赤道纬向风驱动的波动过程是它们存在联系的根本原因。赤道东印度洋地区的西风(东风)强迫出向东传的下沉(上升)的Kelvin波, 在苏门答腊岛西岸转变为沿岸Kelvin波向北传到孟加拉湾的东边界和北边界, 并且在缅甸的伊洛瓦底江三角洲顶部(95°E, 16°N)激发出向西的Rossby波, 造成湾内等温层深度的正(负)异常, 波动传播的速度决定了湾内的变化过程滞后于赤道区域1~2个月。