亚洲大陆边缘海底峡谷的形态、分布及演化进程

基于SRTM15_Plus水深数据,利用地形表面流水分析与等高线几何分析相结合的方法对亚洲大陆边缘海底峡谷进行识别,分析峡谷的形态、分布及演化进程。共识别出海底峡谷531条,按照平面形态分为直线型、蛇曲型和树枝型三种,数量分别为239条、75条和217条。直线型峡谷主要分布在白令海盆地、千岛盆地、南海等海域;蛇曲型峡谷主要分布在孟加拉湾东部俯冲带海域;树枝型峡谷主要分布在日本海沟、伊豆小笠原海沟和南海海槽交界处,南海以及苏门答腊俯冲带海域。根据峡谷的形态与分布,结合研究区内构造背景,讨论了峡谷“直线型-蛇曲型”的形态演化进程,并依据树枝型峡谷主干谷的形态信息,推测存在“蛇曲型-树枝型”、“直线性-树枝型”的形态演化进程。     

漫谈防雷装置检测

回顾了防雷检测的发展历程,阐述了常规检测的必要性及其工作流程,通过防雷装置的质量特点分析了跟踪检测的必要性,并由此引出关于防雷检测标准的问题。参照其它标准及检测实验室质量管理方面的资料,文章着重讨论了新建建筑物防雷跟踪检测的抽样方案和防雷检测机构的质量管理等问题。     

基于熵和转移矩阵分析北京地铁15号线地面沉降时空特征

首先基于PS-InSAR技术获取地铁15号线地面沉降信息,采用信息论方法计算研究区2003-2019年的熵值,然后通过转移矩阵分析地面沉降的转化规律及其与熵值变化的关系。发现2003-2020年,研究区受地质构造影响差异沉降明显,年均地面沉降速率区间为[-100.17,1.19]mm/a;2016年前信息熵不断增加,地面沉降差异性较大,之后信息熵逐渐降低,沉降一致性较好;熵值大小与地面沉降的发育程度有关,沉降严重区熵值较高,沉降稳定区熵值较低;熵值增大时各沉降类型的转出率高,此时地面沉降系统不稳定,熵值减小时各沉降类型的转出率低,沉降系统较稳定。     

Construction of white spot syndrome virus (WSSV) whole genome phage display library

A rebuilt vector pCANTAB 5 EE was obtained by inserting a 34 bp double-stranded oligonucleotide which contained a EcoRV recognition sequence into pCANTAB 5 E. White spot syndrome virus (WSSV) genome DNA was fragmented by sonication to isolate fragments mainly in the range of 0.8~2.0 kb, then the fragments were blunt-ended with T4 DNA polymerase and cloned into the EcoRV site of pCANTAB 5 EE. The primary recombinant clone of the library was 3.0×105. Colony PCR of random selected recombinants showed that the size of the inserts was 0.12~1.77 kb. After the whole library recombinant phages infected Escherichia coli HB2151 cells, the extracellular and periplasmic extracts were dropped on PVDF membranes to perform dot blot, using polyclonal mouse anti-VP24 serum, anti-WSV026 serum, anti-WSV063 serum, anti-WSV069 serum, anti-WSV112 serum, anti-WSV238 serum, anti-WSV303 serum and anti-VP26 serum as the primary antibody, respectively. The results showed that the display library could express the viral proteins.     

黔东南坑头金矿床热液蚀变特征及成矿过程研究

黔东南金成矿区位于江南造山带金成矿省的西南端,成矿条件优越。坑头金矿床是黔东南金成矿区的一个中型矿床,在其深部找矿中,发现除石英脉型矿体外,还存在蚀变岩型矿体。然而,这种蚀变岩型矿体的构造形态、蚀变类型、与石英脉型矿体之间关系和金的赋存状态尚不清楚。本研究与当前的勘查工作紧密结合,围绕石英脉型矿体和新发现的蚀变岩型矿体为研究切入点,借助微区分析技术(扫描电镜和电子探针)进行系统的“流体-蚀变-成矿”研究。蚀变矿物金红石矿物化学显示为热液成因,具有典型造山型金矿床的金红石标型特征。围岩的沉积-成岩过程(包括低级变质作用过程),主要形成了草莓状黄铁矿和含铁碳酸盐岩,为后期含金硫化物(黄铁矿和毒砂)的形成提供物质基础(如Fe)。金的成矿富集过程主要经历了绢云母+毒砂+黄铁矿+石英(Ser+Apy+Py+Qtz)阶段、黄铁矿+毒砂+石英(Py+Apy+Qtz)阶段和自然金+石英(Au⁰+Qtz)阶段。在Ser+Apy+Py+Qtz阶段,主要表现为含矿流体与围岩的初级交代,形成大量浸染状黄铁矿+毒砂的硫化带;Py+Apy+Qtz阶段主要为流体沿着剪切带再交代,形成蚀变岩型矿...     

基于GIS的车载信息服务系统的研究

随着GPS定位技术、数据通信技术、地理信息系统的不断发展以及在交通领域的扩展应用,智能交通系统的建立成为必然,基于GIS技术的车载信息服务应运而生,并发挥出了重要作用,探讨了建立车载信息服务系统的主要内容及各项关键技术。     

华东地区能见度时空变化特征及影响因子分析

为探究华东地区能见度变化情况,利用1973—2020年的能见度数据、2014—2019年的气象要素和污染物浓度数据,采用趋势分析、经验正交函数(Empirical Orthogonal Function, EOF)分解、相关分析的方法,分析了能见度时空变化特征及影响因子。结果表明：(1)1973—2020年能见度呈显著(p<0.01)下降趋势,变化倾向率为-1.315 km/10a,季节之间存在差异,夏、秋季能见度下降速率较大分别为1.681 km/10a、1.443 km/10a;冬、春季下降幅度相对较小分别为1.092 km/10a、1.091 km/10a。其中,1973—2012年能见度呈显著(p<0.01)下降趋势,变化倾向率为-1.204 km/10a, 2013—2020年能见度呈不显著(p>0.05)增加趋势,变化倾向率为2.229 km/10a,近8年(2013—2020年)来能见度存在明显改善。(2)华东地区南部、北部能见度较好,中部能见度较差。EOF分解第1模态表明华东地区能见度整体变化趋势一致,第2模态具有明显的区域差异。(3)能见度与相对湿度...     

His260残基的替代改变食鹿角菜假交替单胞菌芳香基硫酸酯酶的热稳定性

This study aimed to improve the thermostability of arylsulfatase from Pseudoalteromonas carrageenovora. A library of P. carrageenovora arylsulfatase mutants was constructed by introducing random mutagenesis using error-prone PCR. After screening, two mutants of H260 L and D84 A/H260 L showed enhanced thermal stability than the wild-type predecessor(WT). Site-directed mutagenesis demonstrated that only amino acid residue at Position 260 plays an important role in the thermostability of P. carrageenovora arylsulfatase. Thermal inactivation analysis showed that the half-life(t_(1/2)) values at 55°C for H260 L, H260 I, H260 Q, H260 F and H260 R were 40.6, 48.4, 30.9, 29.1 and 34.5 min, respectively, while that of WT was 9.1 min. Structure modeling demonstrated that the additional hydrogen bonds and/or optimization of surface charge-charge interactions could be responsible for the increased thermostability imparted by H260 L, H260 I, H260 Q, H260 F and H260 R.     

Distribution and Ecological Risk Assessment of HCHs and DDTs in Surface Seawater and Sediment of the Mariculture Area of Jincheng Bay,China

The distribution of hexachlorocyclohexanes(HCHs) and dichlorodiphenyltrichloroethanes(DDTs) in the surface seawater and sediment of Jincheng Bay mariculture area were investigated in the present study. The concentration of total HCHs and DDTs ranged from 2.98 to 14.87 ng L-1 and were 0.032 ng L-1, respectively, in surface seawater, and ranged from 5.52 to 9.43 and from 4.11 to 6.72 ng g-1, respectively, in surface sediment. It was deduced from the composition profile of HCH isomers and DDT congeners that HCH residues derived from a mixture of technical-grade HCH and lindane whereas the DDT residues derived from technical-grade DDT and dicofol. Moreover, both HCH and DDT residues may mainly originate from historical inputs. The hazard quotient of α-HCH, β-HCH, γ-HCH and δ-HCH to marine species was 0.030, 0.157, 3.008 and 0.008, respectively. It was estimated that the overall probability of adverse biological effect from HCHs was less than 5%, indicating that its risk to seawater column species was low. The threshold effect concentration exceeding frequency of γ-HCH, p,p'-DDD, p,p'-DDE and p,p'-DDT in sediment ranged from 8.3% to 100%, and the relative concentration of the HCH and DDT mixture exceeded their probable effect level in sediment. These findings indicated that the risk to marine benthos was high and potentially detrimental to the safety of aquatic products, e.g., sea cucumber and benthic shellfish.