Characterization of a thermostable manganese-containing superoxide dismutase from inshore hot spring thermophile Thermus sp. JM1

A thermostable superoxide dismutase (SOD) from the inshore thermophile Thermus sp. JM1 was purified to homogeneity by steps of fractional ammonium sulfate precipitation, DEAE-Sepharose chromatography and Phenyl-Sepharose chromatography. The specific activity of the purified native enzyme was 1 656 U/mg. A sod gene from this strain was cloned and overexpressed in Escherichia coli (E. coli). The prepared apo-enzyme of the purified recombinant SOD (rSOD) was reconstituted with either Fe or Mn by means of incubation with appropriate metal salts. As a result, only Mn 2+ - reconstituted rSOD (Mn-rSOD) exhibited the specific activity of 1 598 U/mg. SOD from Thermus sp. JM1 was Mn-SOD, judging by the specific activities analysis of Fe or Mn reconstituted rSODs and the insensitivity of the native SOD to both cyanide and H 2 O 2 . Both the native SOD and Mn- rSOD were determined to be homotetramers with monomeric molecular mass of 26 kDa and 27.5 kDa, respectively. They had high thermostability at 50 ° C and 60 ° C, and showed striking stability across a wide pH span from 4.0 to 11.0.     

Overexpression and characterization of a thermostable β-agarase producing neoagarotetraose from a marine isolate Microbulbifer sp.AG1

An agarase gene containing 1 302 bp was cloned from Microbulbifer sp. AG1. It encoded a mature protein of 413 amino acids plus a 20-residue signal peptide. The recombinant enzyme without the signal peptide was expressed and purified from Escherichia coli BL21(DE3). When agarose was used as a substrate, the optimal temperature and pH for the enzyme were 60℃ and 7.5, respectively. The recombinant agarase showed excellent thermostability with 67% and 19% of residual activities after incubation at 50℃ and 60℃ for 1 h, respectively.Except SDS, the recombinant agarase had a relatively good resistance against the detected inhibitors, detergents and urea denaturant. Thin layer chromatography analysis and enzyme assay using p-nitrophenyl-α/β-Dgalactopyranoside revealed that the recombinant agarase was a β-agarase that degraded agarose into neoagarotetraose as the main end product. The enzymatic hydrolysis products with different degree of polymerization exhibited the antioxidant activities.     

深水锚在海床中复杂动力行为的分析模型

深水锚是深水系泊系统的核心组成,随着新概念和新技术在深水系泊领域的不断涌现和发展,深水锚在海床中的动力行为变得更为复杂,这给既有的分析方法带来极大的挑战。将深水锚的复杂动力行为视为拔出、旋转、嵌入三种典型行为的组合,分别给出明确定义并建立力学模型。提出最小力准则以确定锚的拔出、嵌入方向和旋转中心,该准则同时可作为拔出、旋转、嵌入行为是否发生的判定条件,从而建立起可以描述深水锚在海床中复杂动力行为的分析模型。为检验所提出的分析模型,将其应用于预测拖曳锚在海床中的嵌入运动轨迹,并与已有分析方法和离心机试验结果进行对比,验证了该分析模型的有效性。     

民航西南地区气象雷达信息共享

气象雷达信息共享对于空中交通管制有着重要的作用,本文阐述了使用FTP协议,通过民航ATM,完成民航西南地区气象雷达信息的共享。     

Emergency alternative evaluation and selection based on ANP,DEMATEL, and TL-TOPSIS

With the increasing occurrence frequency of emergency events, emergency management (EM) has been a very important issue in management science. One of the major activities of EM is to evaluate and select the most desirable emergency alternative(s). This paper proposes a new framework combining the analytic network process (ANP) method, the decision-making trial and evaluation laboratory (DEMATEL) technique, and 2-tuple linguistic technique for order preference by similarity to an ideal solution (TL-TOPSIS) method to solve the emergency alternative evaluation and selection problem. This study has been done in three stages. In the first stage, we use DEMATEL technique to obtain the network relation map (NRM) among emergency alternative evaluation criteria or sub-criteria. In the second stage, we use ANP method to calculate the global weight of each sub-criterion based on the NRM among emergency alternative evaluation sub-criteria. In the third stage, the ratings of emergency alternative with respect to each sub-criterion are described by linguistic items, and the TL-TOPSIS method is used to rank the emergency alternative. Finally, a practical example of urban fire emergency alternative selection is given to illustrate the application of the proposed framework.     

中国东南部花岗岩的碳含量和同位素比值

本文应用ＥＡ－ＭＳ连续技术（将元素分析仪（ＥＡ）与气体质谱仪（ＭＳ）连接起来,进行硅酸盐岩石微量碳的含量和同位素比值测定）首次测定了中国东南部花岗岩及其部分磷灰石的全碳含量和同位素组成,结果发现,这些花岗岩的碳含量和同位素组成分布较宽（碳含量为０．０４％～０．７９％,δ＾１３Ｃ值为－７．５‰～－３７．０‰）。磷灰石与全岩的δ＾１３Ｃ呈正相关关系,指示自花岗岩浆上升到结晶以来未受到同化混染或热液蚀变     

Correlation analysis of persistent heavy rainfall events in the vicinity of the Yangtze River valley and global outgoing longwave radiation in the preceding month

Based on the National Oceanic and Atmospheric Administration (NOAA) daily satellite dataset of global outgoing longwave radiation (OLR) for the period of 1974--2004 and the NCEP-NCAR reanalysis for 1971--2004, the linkage between persistent heavy rainfall (PHR) events in the vicinity of the Yangtze River valley and global OLR leading up to those events (with 1- to 30-day lag) was investigated. The results reveal that there is a significant connection between the initiation of PHR events over the study area and anomalous convective activity over the tropical Indian Ocean, maritime continent, and tropical western Pacific Ocean. During the 30-day period prior to the onset of PHR events, the major significantly anomalous convective centers have an apparent dipole structure, always with enhanced convection in the west and suppressed convection in the east. This dipole structure continuously shifts eastward with time during the 30-day lead period. The influence of the anomalous convective activity over the tropical oceans on the initiation of PHR events over the study area is achieved via an interaction between tropical and extratropical latitudes. More specifically, anomalous convective activity weakens the Walker circulation cell over the tropical Indian Ocean first. This is followed by a weakening of the Indian summer monsoon background state and the excitation and dispersion of Rossby wave activity over Eurasia. Finally, a major modulation of the large scale background circulation occurs. As a result, the condition of a phase-lock among major large scale circulation features favoring PHR events is established over the study area.     

昆仑-黄河运动的时代下限:来自塔里木盆地南缘西域砾岩顶部火山岩~(40)Ar/~(39)Ar定年的约束

"昆仑-黄河运动"是发生在早、中更新世之交的一次强烈的构造运动,它使青藏高原现代构造地貌格局基本定型。以往对昆仑-黄河运动时限的研究,主要由黄河干流发育的阶地、昆仑山垭口地区羌塘组与望昆冰碛层等古地磁、ESR、TL等分析测试得出,缺少其他年代学约束。在塔里木盆地,昆仑-黄河运动表现为西域砾岩与乌苏群之间的角度不整合接触。本文通过对塔里木盆地南缘西域砾岩顶部的普鲁上层火山岩4个样品的全岩~(40)Ar/~(39)Ar定年,获得~(40)Ar/~(39)Ar坪年龄为1.03±0.05 Ma~1.20±0.05 Ma,加权平均年龄为1.1±0.1 Ma(n=4,MSWD=2.3)。这是首次应用~(40)Ar/~(39)Ar定年方法,获得昆仑-黄河运动的时代下限为1.1±0.1 Ma,与前人确定的昆仑-黄河运动时代下限在误差范围内一致,为我国西部新构造运动提供了重要的年代学约束。     

PS溶液渗透对非饱和土的增强效应研究

特定模数硅酸钾溶液(PS)已广泛的用于我国西北干旱地区土遗址加固保护工程中,并且取得了显著的效果.但是PS对潮湿地区遗址土加固效果,目前尚缺乏成熟的理论和实验验证.本文从室内试验的资料出发,研究PS对潮湿地区土遗址非饱和土的增强效应.本次研究土样取自位于潮湿环境中的浙江杭州良渚土遗址(3000 B C.),在实验室内对...     

藏北多龙斑岩铜金矿集区综合信息找矿模型研究

藏北多龙矿集区产出环境和成矿动力学背景在青藏高原斑岩铜矿中独具特色,目前已经发现了多龙、多不杂、拿若、荣那等超大型—大型斑岩铜(金)矿,其有效找矿模式亟待研究总结。典型矿床的综合信息找矿模型研究对于进一步开展矿体定位预测具有重要意义。研究认为多龙矿集区铜金矿体产于花岗闪长斑岩体内及岩体边部的黄铁绢英岩化和青磐岩化变质(长石)石英砂岩及火山岩中。自含矿斑岩体中心向外存在中心对称分布的3个蚀变带,依次为弱钾化带、绢英岩化带和黄铁矿化-角岩化带。根据地、物、化、遥异常套合特征,划分出"三带五田"。"三带"为呈北西向雁列状排列的拿若—色那—尕尔勤铜金矿带、多不杂—波龙—铁格隆南铜金钼矿带和地堡那木岗铜金矿带;"五田"为拿若—赛角—荣那矿田、尕尔勤矿田、地堡那木岗矿田、波龙—多不杂矿田和铁格隆南矿田,并分别厘定了各矿田的基本组成与矿床地质特征。通过地、物、化综合异常分析,建立了"四位一体"找矿模式。指出铁帽、孔雀石化和蚀变花岗闪长斑岩体为地表寻找斑岩铜矿的直接标志;低磁异常间接反映遭受蚀变的花岗闪长斑岩体成矿母岩;激电高极化异常直接反映斑岩型铜矿体两翼的黄铁矿化带,间接反映硫化物带包裹着铜矿体;Cu、Au元素高异常反映成矿元素的区域富集。以此为基础,对拿若、荣那矿区掩埋矿体运用了"四位一体"找矿模式,发现隐伏斑岩型铜矿床。