High-resolution measurements of chromophoric dissolved organic matter in the Mississippi and Atchafalaya River plume regions

Chromophoric dissolved organic matter (CDOM) was measured in the spring and summer in the northern Gulf of Mexico with the ECOShuttle, a towed, instrumented, undulating vehicle. A submersible pump mounted on the vehicle supplied continuously flowing, uncontaminated seawater to online instruments in the shipboard laboratory and allowed discrete samples to be taken for further analysis. CDOM in the northern Gulf of Mexico was dominated by freshwater inputs from the Mississippi River through the Birdfoot region and to the west by discharge from the Atchafalaya River. CDOM was more extensively dispersed in the high-flow period in the spring but in both time periods was limited by stratification to the upper 12 m or so. Thin, subsurface CDOM maxima were observed below the plume during the highly stratified summer period but were absent in the spring. However, there was evidence of significant in situ biological production of CDOM in both seasons.The Mississippi River freshwater end member was similar in spring and summer, while the Atchafalaya end member was significantly higher in the spring. In both time periods, the Atchafalaya was significantly higher in CDOM and dissolved organic carbon (DOC) than the Mississippi presumably due to local production and exchange within the coastal wetlands along the lower Atchafalaya which are absent along the lower Mississippi. Nearshore waters may also have higher CDOM due to outwelling from coastal wetlands. High-resolution measurements allow the differentiation of various water masses and are indicative of rapidly varying (days to weeks) source waters. Highly dynamic but conservative mixing between various freshwater and marine end members apparently dominates CDOM distributions in the area with significant in situ biological inputs (bacterial degradation of phytoplankton detritus), evidence of flocculation, and minor photobleaching effects also observed. It is clear that high-resolution measurements and adaptive sampling strategies allow a more detailed examination of the processes that control CDOM distributions in river-dominated systems.     

Roles of Continental Shelves and Marginal Seas in the Biogeochemical Cycles of the North Pacific Ocean

Most marginal seas in the North Pacific are fed by nutrients supported mainly by upwelling and many are undersaturated with respect to atmospheric CO₂ in the surface water mainly as a result of the biological pump and winter cooling. These seas absorb CO₂ at an average rate of 1.1 ± 0.3 mol C m⁻²yr⁻¹ but release N₂/N₂O at an average rate of 0.07 ± 0.03 mol N m⁻²yr⁻¹. Most of primary production, however, is regenerated on the shelves, and only less than 15% is transported to the open oceans as dissolved and particulate organic carbon (POC) with a small amount of POC deposited in the sediments. It is estimated that seawater in the marginal seas in the North Pacific alone may have taken up 1.6 ± 0.3 Gt (10¹⁵ g) of excess carbon, including 0.21 ± 0.05 Gt for the Bering Sea, 0.18 ± 0.08 Gt for the Okhotsk Sea; 0.31 ± 0.05 Gt for the Japan/East Sea; 0.07 ± 0.02 Gt for the East China and Yellow Seas; 0.80 ± 0.15 Gt for the South China Sea; and 0.015 ± 0.005 Gt for the Gulf of California. More importantly, high latitude marginal seas such as the Bering and Okhotsk Seas may act as conveyer belts in exporting 0.1 ± 0.08 Gt C anthropogenic, excess CO₂ into the North Pacific Intermediate Water per year. The upward migration of calcite and aragonite saturation horizons due to the penetration of excess CO₂ may also make the shelf deposits on the Bering and Okhotsk Seas more susceptible to dissolution, which would then neutralize excess CO₂ in the near future. Further, because most nutrients come from upwelling, increased water consumption on land and damming of major rivers may reduce freshwater output and the buoyancy effect on the shelves. As a result, upwelling, nutrient input and biological productivity may all be reduced in the future. As a final note, the Japan/East Sea has started to show responses to global warming. Warmer surface layer has reduced upwelling of nutrient-rich subsurface water, resulting in a decline of spring phytoplankton biomass. Less bottom water formation because of less winter cooling may lead to the disappearance of the bottom water as early as 2040. Or else, an anoxic condition may form as early as 2200 AD. This revised version was published online in July 2006 with corrections to the Cover Date.     

Examination of empirical formulas for wave shoaling and breaking on steep slopes

Estimation of the wave height transformation of shoaling and breaking is essential for the nearshore hydrodynamics and the design of coastal structures. Many empirical formulas have been well recognized to the wave height transformation, but most of them were only applicable for gentle slopes. This paper reports the experimental results of wave shoaling and breaking over the steep slopes to examine the applicability of the previous empirical formulas. Two steep bottom slopes of 1/3 and 1/5, and one gentle slope of 1/10 were conducted in the present experiments. Experimental results show that the shoaling distance of steep slopes become short and the surface waves may be partially reflected from the steep bottom, thus the estimation of wave shoaling using the well-known previous formula did not conform completely to the experimental results. The previous empirical formulas for the wave breaking criteria were also examined, and the modified equations to the steep beaches were proposed in this work. A numerical model was finally adopted to calculate the wave height transformation in the surf zone by introducing the modified breaking index.     

DNA分子标记在水生动物遗传学上的研究进展

遗传变异是生物的重要特征之一，它决定着生物的存在和发展，因而成为人类一直极力要揭开其奥秘并进行能动性改造的方面之一。随着分子生物学理论和技术的发展，以及与水生生物遗传学的相互渗透，近十几年来，分子标记、基因克隆和基因转移技术取得了惊人的进展，一些新基因目前已稳定地转人多种水生生物中。目前分子标记辅助选择育种和基因转移已成为培育新品种的有效手段。与主要的农作物相比，水生生物大多数性状均表现为数量遗传、个体基因组高度杂合、进行传统的遗传育种非常耗资、费时和费力。分子生物学技术的介入，特别是DNA分子标记技术的发展和应用，对水生动物遗传学研究起到了巨大的推动作用。DNA分子标记大多是以DNA片段的电泳谱带形式表现的。依据其遗传特性，可分为显性和共显性标记两种。依据其在基因组中的出现频率，又可分为低拷贝序列标记和重复序列标记。依其多态性检出所用的分子生物学技术，可分为以Southern杂交技术为基础的分子标记，     

我国海洋软相底质底栖经济贝类的可持续利用探讨

分析了海洋软相底质底栖贝类的资源特性和我国的资源利用现状及存在问题．并从规模化苗种生产、海区增殖、高效养殖模式开发及增养殖环境保障与产品无公害生产等方面对该资源的可持续利用进行了探讨。     

山东省设施农业生产季连阴天时空演变特征及其影响

利用山东省59个国家级气象观测站1961—2018年逐日日照时数及32个设施农业小气候观测站2008—2018年逐日气温观测资料,采用线性回归法、Mann-Kendall突变检验法及反距离权重插值法等分析了设施农业生产季连阴天时空分布和变化规律及其对设施内气温条件的影响。结果表明,时间上,过去58 a山东省连阴天年总发生次数和总连阴天数分别以1.7次·a~(-1)和6.5d·a~(-1)的趋势增加,且年总发生次数在1999年发生突变;空间上,过去58 a连阴天总发生次数和总连阴天数均呈"东北—西南"向增多分布,聊城和德州地区的增加较其他区域更为明显,单站年发生次数和连阴天数增加超过0.06次·a~(-1)和0.2 d·a~(-1),且变化趋势在聊城等15个和13个站点通过显著性水平为0.05的显著性检验;随着连阴天数增加,设施内气温降温幅度会增大;相同连阴天数时,平均气温和最低气温降幅春秋季大于冬季,分析其原因可能是春秋季基础温度高于冬季,从而更容易引起温度的剧烈变化所导致。研究结果可为有关部门研究设施农业种植布局和指导设施农业生产及防灾减灾提供理论支持。     

富含半胱氨酸肠蛋白的研究进展

富含半胱氨酸肠蛋白是一种含双锌指结构的小分子蛋白，其在锌离子转运、个体发育、免疫防御以及肿瘤发生等多种生理过程中发挥重要作用。作者主要对富含半胱氨酸肠蛋白的结构特点及其在发育和免疫方面的研究进展进行系统概述，并指出其相关作用机制尚待深入研究。     

A new pelagic Polyconchoecia Xiang, Chen and Du, 2018 (Ostracoda: Myodocopa: Halocyprididae) from the South China Sea

Halocyprid ostracods are appreciable part of ostracods floating through virtually everywhere in marine environment.In this study,we describe a new species of genus Polyconchoecia Xiang,Chen and Du,2018,tribe Conchoeciini Chavtur and Angel,2011,family Halocyprididae Dana,1853 from the middle of the South China Sea.Polyconchoecia chenii sp.nov.is very close to P.commixtus Xiang,Chen and Du,2018.But it differs from P.commixtus by the distinctions of locations of major glands of carapace and the characteristics of appendages:more posteriorly situated left asymmetric gland of carapace,no right asymmetric gland;segmented frontal organ;the endopod 2 of the first antenna with a very small seta;a-and c-setae of the first antenna with long end joint have long end joint,the b-and d-setae have no end joint,spinose e-seta without end joint;the e-seta of the second antenna is present;teeth side is distinctive;the setal counts of the mandible,maxilla,fifth limb,and sixth limb are individual.The locations of the major glands on carapace and the characteristics of the first antenna can be the key of the new species.This work is the second discovery of the genus Polyconchoecia from the world.     

军曹鱼(Rachycentron canadum)幼鱼对环境低氧胁迫氧化应激与能量利用指标的响应

本文探究环境低氧对军曹鱼(Rachycentron canadum)氧化应激和能量利用指标的影响,为军曹鱼的健康养殖提供参考依据。通过设置低氧胁迫–恢复实验,将军曹鱼幼鱼(平均体质量(220.67±20.73)g)在低氧((2.64±0.25)mg/L)胁迫3 h及复氧((6.34±0.15)mg/L)8 h、24 h和48 h后,测定其肝脏和肌肉组织的氧化应激与能量利用指标。结果显示,低氧胁迫后,肝脏中丙二醛(Malondialdehyde,MDA)、过氧化氢酶(Catalase,CAT)和谷胱甘肽还原酶(Glutathione Reductase,GR)活力均显著低于对照组(p<0.05),乳酸脱氢酶(Lactate Dehydrogenase,LDH)活性显著高于对照组(p <0.05);肌肉中MDA和脂质过氧化物(Lipid Peroxidase,LPO)活性均显著低于对照组(p<0.05),超氧化物歧化酶(Superoxide Dismutase,SOD)和LDH活性均显著高于对照组(p<0.05);肌糖原和肝糖原含量极显著低于对照组(p<0.0...     

近30年长江口海域生态系统健康状况及变化趋势研究

为评估长江口海域生态系统健康状况,本文以鱼类浮游生物为指示生物,选择1986年、1999年、2007年和2016年4个年份的数据,选取鱼类浮游生物总种类数、底栖鱼种类数、水层鱼种类数、低耐污鱼种类数、高耐污鱼种类数百分比、杂食性鱼种类数百分比、虫食性鱼种类数百分比、肉食性鱼种类数百分比、鱼类取样个体数、天然杂交种种类数百分比等10个评价指标,采用1、3、5赋值法计算4个不同年份的河口生物完整性指数(Estuarine Biotic Integrity Index,EBI),并以此评价近30年长江口海域生态系统健康状况变化趋势。结果显示,1986年、1999年、2007年和2016年4个不同年份的EBI值分别是52、40、36、34,对应的EBI等级分别为“好”、“一般”、“一般−差”、“差”。通过分析EBI年际变化的结果,发现近30年来长江口海域生态系统健康状况呈现先下降,而后稳定在较低水平的趋势,说明长江口海域生态系统亟需及时进行保护和修复工作。